Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,270,143 C→T 5.4% intergenic (+541/+73) yhaC → / ← rnpB pentapetide repeats‑related protein/RNase P, M1 RNA component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,270,1430CT 5.4% 536.6 / 16.0 187intergenic (+541/+73)yhaC/rnpBpentapetide repeats‑related protein/RNase P, M1 RNA component
Reads supporting (aligned to +/- strand):  ref base C (111/61);  new base T (3/7);  total (115/72)
Fisher's exact test for biased strand distribution p-value = 4.15e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.63e-01

Only 100 of 192 total aligned reads displayed.

ACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAA  >  NC_000913/3269997‑3270291
                                                                                                                                                  |                                                                                                                                                    
  tGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGcgtcgt                                                                                                                                                 <  2:1918425/150‑1 (MQ=37)
  tGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTGTGGGGTAGAGcgtcgt                                                                                                                                                 >  2:19093/1‑150 (MQ=255)
  tGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCAATCATATTTCTTCCCCGCCAAAAGCAAAAAACCCCCCAACCGGGTTTTTACGTAAAACAGGTGAAACTGACCCATAAGCCCCGTTCTTGTGGGTATAGcgtcgt                                                                                                                                                 >  1:1613399/1‑150 (MQ=11)
      cGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTAAGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTAAAGCGTCGTGGaa                                                                                                                                             >  2:3620371/1‑149 (MQ=12)
                            tagaGTGTCGTGGACACGCATTCATCTTTCTGCCCCTCCAACAGCACAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCt                                                                                                                       <  1:1023991/147‑1 (MQ=11)
                              taGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTcc                                                                                                                     <  1:2782008/150‑1 (MQ=37)
                                  gtCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCaaaa                                                                                                                 <  1:3011302/150‑1 (MQ=37)
                                     gTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATAGATCTATATGGGCGTCCAAAAGCa                                                                                                              >  2:3537121/1‑150 (MQ=11)
                                      tGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCaa                                                                                                             >  1:718434/1‑150 (MQ=37)
                                        gACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCaaaa                                                                                                           <  2:4131426/150‑1 (MQ=37)
                                         aCAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGTCCCTCCAAAAGCaaaaa                                                                                                          <  2:1638981/150‑1 (MQ=37)
                                          cAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGGGGCCAGTCAATCAAAATTCTGCCCCGACAAAATCCAAAAc                                                                                                         >  2:3491737/1‑150 (MQ=11)
                                           aGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCaa                                                                                                                   <  2:51625/139‑1 (MQ=17)
                                           aGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGTCATTCATCTGTCTGCCCCTCCAAAAGCAAAAAcc                                                                                                        >  1:3768570/1‑150 (MQ=255)
                                           aGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAAcc                                                                                                        >  1:4102916/1‑150 (MQ=37)
                                           aGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCATTCTGCCCCTCCAAAAGCAAAAAcc                                                                                                        >  1:2147616/1‑150 (MQ=37)
                                             tcattcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACccg                                                                                                      >  1:71222/1‑150 (MQ=37)
                                             tcattcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCGCCAAAAGCAAAAACccg                                                                                                      >  1:2157288/1‑150 (MQ=37)
                                              cattcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACccgc                                                                                                     <  1:4114820/150‑1 (MQ=37)
                                                ttcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTTCCCCGCCAAAAGCAAAAACccgccg                                                                                                   >  1:3642001/1‑150 (MQ=255)
                                                 tcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGa                                                                                                  >  1:2491911/1‑150 (MQ=37)
                                                 tcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATATTTCTGCCCCTCCAAAAGCAAAAACCCGCCGa                                                                                                  >  2:2246813/1‑150 (MQ=37)
                                                   atCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAg                                                                                                >  2:46370/1‑150 (MQ=37)
                                                   atCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCAACTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAg                                                                                                >  2:1948748/1‑150 (MQ=37)
                                                   atCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCGTGCTTTTGGGTAGAGCGTCGTGGACAGTCAATCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAg                                                                                                >  2:3800717/1‑150 (MQ=255)
                                                    tCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGc                                                                                               >  1:3146678/1‑150 (MQ=37)
                                                    tCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGGCATTCATCTTTCTGCCCCGCCAAAAGCAAAAACCCGCCGCAGc                                                                                               >  2:3587048/1‑150 (MQ=255)
                                                     cTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCg                                                                                              >  2:3703843/1‑150 (MQ=37)
                                                     cTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTAGGCCCCACCAAAACCAAAAACCCGCCGAAGCg                                                                                              >  2:1529411/1‑150 (MQ=255)
                                                     cTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATATTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCg                                                                                              >  2:1554897/1‑150 (MQ=37)
                                                     cTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTGAGGGTAAAGCGTCGTGGACACTCATTCATATTTATGCACCTCCAAAAGCAAAAACCCGCCGAAGCg                                                                                              >  2:3284615/1‑150 (MQ=11)
                                                      tttCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCgg                                                                                             >  1:2055129/1‑150 (MQ=37)
                                                         cgcgcccTCCACAAGCACAAACCCGCCGCATCGGGTTATTACGTAAACCAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGtt                                                                                          <  2:1647728/146‑1 (MQ=11)
                                                          ggCCCCTCCACAAGCAAAAACCCGCCGAAGCGGTTTTTTACGTAAAACAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGttt                                                                                         <  1:324745/149‑1 (MQ=255)
                                                            ccccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGttttt                                                                                       >  1:1339217/1‑150 (MQ=37)
                                                             cccTCCACCCGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCATCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTa                                                                                      <  2:4076740/150‑1 (MQ=255)
                                                             cccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTa                                                                                      <  2:2373992/150‑1 (MQ=37)
                                                             cccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATATTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTa                                                                                      >  2:2707308/1‑150 (MQ=37)
                                                              ccTCCACAATCATAAACCCGCCGCACCGGGTTATTACGTACACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTAc                                                                                     <  2:2394687/150‑1 (MQ=11)
                                                              ccTCCACAATCAAAAACCCGCCGAATCGGTTTATTACGTAAACCAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTAc                                                                                     <  1:925769/150‑1 (MQ=11)
                                                              ccTCCAAAATCAAAAACCCGCCGTAGGGGTTTTTTACGTAAACCTGGTTAAACTGACCTATAAGCCGCTTTCTTTTGGGTATAGGGTCGTGGACAGTCATTCTTCTTTCTGCCCCTCCAAAAGAAAAAACCCGCTTCGGCGGGtttttgc                                                                                     <  1:1395649/150‑3 (MQ=11)
                                                              ccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTAc                                                                                     >  1:1712803/1‑150 (MQ=37)
                                                              ccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTGTTTAc                                                                                     >  2:2144106/1‑150 (MQ=37)
                                                               ccccAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCTATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCTTCGGCGGGtttttgct                                                                                    <  1:435077/148‑4 (MQ=11)
                                                                tCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTACCAAAGCAAAAACCCGCCGAAGCGGGTTTTTACgg                                                                                   >  2:3302339/1‑149 (MQ=255)
                                                                tCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCGCCAAAAGCAAAATCCCGCCGAAGCGGGTTTTTACgg                                                                                   >  2:115014/1‑149 (MQ=255)
                                                                  cacaaGCAAAAACCCGCCGTAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTaa                                                                                 <  2:1265198/147‑1 (MQ=255)
                                                                  cAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGTCATTCATCGTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTTCGTaa                                                                                 >  2:939379/1‑150 (MQ=255)
                                                                  cAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCACAAACCCGCCGCAGCGGGTTGTTACGTaa                                                                                 >  2:2774413/1‑150 (MQ=255)
                                                                  cAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCGGCCCCTCCAAAAGCAAAAACCCGCCGCAGCGGGTTTTTACGTaa                                                                                 >  2:2922594/1‑150 (MQ=255)
                                                                  cAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTGTTGGGTATCGCGTCGTGGACCGGCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGCAGCGGGTGTGGACGTaa                                                                                 >  2:3045572/1‑150 (MQ=11)
                                                                    caaGCAAAAACCCGCCGCAGCGGGTTTTTACGTAACCCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAt                                                                               <  2:386508/149‑1 (MQ=255)
                                                                    aaaGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCAGCCCCTCCAAAAGCAAAAAACCGCCGAAGCGGGTTTTTAAGTAAAt                                                                               >  2:493103/1‑150 (MQ=255)
                                                                        cAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGGTTTTACGGAAATCAgg                                                                           >  2:2512771/1‑150 (MQ=255)
                                                                          aaatCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGGTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTGTGCCCCTGCAAAAGCAATATCCCGCCGAAGCGGGTTTTTAGGTTAATCAGGTg                                                                         >  2:3108589/1‑150 (MQ=11)
                                                                             aCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGaaa                                                                      >  1:336991/1‑150 (MQ=37)
                                                                              cccGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAAc                                                                     >  2:2588774/1‑150 (MQ=37)
                                                                                cgccgAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATGTTTGTGCCCCTACAAAATCAAAAACCCGCCGAAGCGGGTTTTTTAGTAAATAAGGTGtaaatt                                                                   >  2:3967550/1‑147 (MQ=11)
                                                                                  ccgCAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGAc                                                                 <  2:2822298/150‑1 (MQ=37)
                                                                                        cGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATaa                                                           <  1:450930/150‑1 (MQ=21)
                                                                                        cGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATaa                                                           >  2:4031142/1‑150 (MQ=37)
                                                                                        cGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATaa                                                           >  2:2965284/1‑150 (MQ=37)
                                                                                        cGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTAAGTAAATAAGTTGAAACTGTCCGATaa                                                           >  2:3165312/1‑150 (MQ=255)
                                                                                               ttACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCGCCAAAAGCAAAAACCCGCCGCAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGt                                                    >  1:1563576/1‑150 (MQ=255)
                                                                                                  cGTACACCAGGTGAAACGGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCCTCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGCAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCt                                                 <  2:3602961/150‑1 (MQ=255)
                                                                                                       aCCAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCAGTCATCTTTCTGCCCCCCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGt                                            <  2:2887713/150‑1 (MQ=255)
                                                                                                          tgttGAAACTGACAGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGa                                         <  1:3157142/147‑1 (MQ=255)
                                                                                                          aGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGa                                         <  2:4102916/150‑1 (MQ=255)
                                                                                                            gtaaaaCTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACa                                       <  2:276745/147‑1 (MQ=255)
                                                                                                               cctcTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGtc                                    <  1:3928060/147‑1 (MQ=255)
                                                                                                               aaaCTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCgg                                                      <  1:610937/132‑1 (MQ=11)
                                                                                                               aaaCTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCgg                                                      >  2:610937/1‑132 (MQ=11)
                                                                                                                 aCTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCGCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGtcat                                  >  2:815979/1‑150 (MQ=255)
                                                                                                                 aCTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCGCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGtcat                                  >  1:2148502/1‑150 (MQ=255)
                                                                                                                   tGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGGCAGTCATTCATCGTTATGCCCCACCAAAAGCAAAAAACCGCCGAAGCGGGTTTTTAAGTAAATCAGGTGAAACAGACCGATAAGCCGGGTTATGTCGTGGTCAGtgattc                                >  1:3464119/1‑150 (MQ=14)
                                                                                                                   tGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCGCCAAAAGCAAAAACCCCCCCAAGCGGGTTTTTACGTAAATCAGGTGAAACTTACCGATAAGCCGGGGTCTGTCGTGGACAGtcattc                                >  2:1115505/1‑150 (MQ=17)
                                                                                                                       cGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAAc                                                                     <  1:2596530/109‑1 (MQ=35)
                                                                                                                          tAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAATCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATAAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAgg                         >  2:1788848/1‑150 (MQ=255)
                                                                                                                           aaGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGa                                         >  2:3157481/1‑133 (MQ=255)
                                                                                                                             gCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCa                      <  1:2306728/150‑1 (MQ=255)
                                                                                                                             gCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCa                      <  1:2577327/150‑1 (MQ=255)
                                                                                                                             gCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCa                      >  2:3852078/1‑150 (MQ=255)
                                                                                                                             gCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCa                      <  1:1826771/150‑1 (MQ=255)
                                                                                                                              ccGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTACACCAGCAAAATCCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAATCTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAg                     <  1:1357553/150‑1 (MQ=17)
                                                                                                                              ccGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAg                     <  1:4031142/150‑1 (MQ=255)
                                                                                                                              ccGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAg                     <  1:2965284/150‑1 (MQ=255)
                                                                                                                              ccGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAg                     <  1:3165210/150‑1 (MQ=255)
                                                                                                                              ccGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCACCGAAGCgg                                                                                             <  1:3513548/78‑1 (MQ=21)
                                                                                                                              ccGCTTTCTTTTGGGTATAGCGTCGTGGACAGGCATTCATCATTATGCCCCACCAAAATCAAAAACCCGCCCCAGCCGGTTTTTAAGTAAAACAGGTGAAAAAGAACCAAAATCCCGGTTCAGGCGTGGAAAGTCAATAATCAAGGCCac                     >  1:240925/1‑149 (MQ=9)
                                                                                                                               cGCTTTCTTTTGGGTATAGAGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGATTCTGTCGTGGACAGTCATTCATCTAGGCCAGc                    <  2:2658111/150‑1 (MQ=255)
                                                                                                                                gCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCa                   >  2:3770754/1‑150 (MQ=255)
                                                                                                                                gCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCa                   >  2:1400882/1‑150 (MQ=255)
                                                                                                                                 ctttctttTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCaa                  <  1:2237492/150‑1 (MQ=255)
                                                                                                                                  tttctttTGGGTATAGCGTCGTGGGCAGTCATTCATCTTTATGCCCCGCCAAAAGCAAAAAACCCCCGAAGCGGGTTTTTACGTAAATAAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGGCCTTAATCTAGGCCAGCaaa                 >  1:1784970/1‑149 (MQ=17)
                                                                                                                                  tttctttTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCCAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGTCAGTCATTCATCTAGGCCAGCAAt                 >  1:3998052/1‑150 (MQ=255)
                                                                                                                                      ttttGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCCGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACCGTCATTCATCTAGGCCAGCAATCGCt             >  2:3213195/1‑150 (MQ=255)
                                                                                                                                      ttttGGGTATAGCGTCGTGGACAGTCATTAATATTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAATCCGGGTTCTGTCGTGGACAGTCATTCATCTATTCCAGCAATCGCt             >  1:2894525/1‑150 (MQ=18)
                                                                                                                                        ttGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGTAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCa           <  1:3091901/150‑1 (MQ=255)
                                                                                                                                        ttGGGTATAGCGTCGTGGACAGTCAATCATATTTATGCCCCACCAAAAGCAAAAAACCCCCGAAGCGGGTTTTTACGTAAAAAAGGTGAAACTGACCGATAAACCGGGTTCTTTCGTGGACAGTCATTCATCTAGGACAGCAATAGCTCa           >  1:18811/1‑150 (MQ=18)
                                                                                                                                                 gCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACAGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCaa  >  2:1491252/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
ACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAA  >  NC_000913/3269997‑3270291

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: