Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,781,022 T→G 8.2% intergenic (+5/‑193) lldD → / → trmL L‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,781,0220TG 8.2% 530.1 / 8.3 182intergenic (+5/‑193)lldD/trmLL‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base T (44/119);  new base G (17/2);  total (61/121)
Fisher's exact test for biased strand distribution p-value = 1.50e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 185 total aligned reads displayed.

CTGATCGAAAAAGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCG  >  NC_000913/3780874‑3781165
                                                                                                                                                    |                                                                                                                                               
cTGATCGAAACAGAGATGAAAGGGGCGCTGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGttt                                                                                                                                                <  2:151665/150‑1 (MQ=255)
 tGATCGAAAAAGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTg                                                                                                                                               >  2:687302/1‑150 (MQ=255)
  gATCGAAAAAGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTgc                                                                                                                                              >  1:3869515/1‑150 (MQ=255)
   aTCGAAAAAGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGACTGCGGCATAGTCGTGTgcg                                                                                                                                             >  1:1953838/1‑150 (MQ=255)
   aTCGAAAAAGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTgcg                                                                                                                                             >  1:1969125/1‑150 (MQ=255)
    tCGAAAAAGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTgcgc                                                                                                                                            >  1:1051995/1‑150 (MQ=255)
    tCGAAAAAGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGACTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTgcgc                                                                                                                                            >  1:2106061/1‑150 (MQ=255)
    tCGAAAAAGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATAAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTtcgc                                                                                                                                            >  1:2504869/1‑150 (MQ=255)
       aaaaaGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGcccc                                                                                                                                         <  1:1665384/150‑1 (MQ=255)
        aaaaGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGccccc                                                                                                                                        <  1:351644/150‑1 (MQ=255)
        aaaaGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGccccc                                                                                                                                        <  1:2214213/150‑1 (MQ=255)
           agagATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCa                                                                                                                                     <  2:2292961/150‑1 (MQ=255)
            gagaTGAAAGTGGCGATGACGCTGACTGGCGCGACATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCAc                                                                                                                                    <  1:3278119/150‑1 (MQ=255)
              gaTGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTTGGCCCCCGCAccc                                                                                                                                  >  2:3106885/1‑150 (MQ=255)
               aTGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCCCAccca                                                                                                                                 >  1:1151370/1‑149 (MQ=255)
                 gAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCACACCCaaa                                                                                                                               >  1:2463500/1‑150 (MQ=255)
                   aaGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAGTGCGGCATAGTCGTTTGCGCCCCCTCACCCTAAcc                                                                                                                             <  2:4131809/150‑1 (MQ=255)
                   aaGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAAcc                                                                                                                             <  2:2734771/150‑1 (MQ=255)
                   aaGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAAcc                                                                                                                             <  1:3490217/150‑1 (MQ=255)
                       ggCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCGCCCCCACAcccacg                                                                                                                         >  1:2178846/1‑147 (MQ=255)
                         cGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTcc                                                                                                                       <  2:4133438/150‑1 (MQ=255)
                          gATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCGCACCCGAACCCACTccc                                                                                                                      >  1:238907/1‑150 (MQ=255)
                             gACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCa                                                                                                                   <  1:332554/150‑1 (MQ=255)
                             gACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCa                                                                                                                   <  1:670261/150‑1 (MQ=255)
                              aCGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAg                                                                                                                  <  2:2734560/150‑1 (MQ=255)
                              aCGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAg                                                                                                                  <  2:619741/150‑1 (MQ=255)
                               cGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAgg                                                                                                                 <  1:2621190/150‑1 (MQ=255)
                                 gtGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGga                                                                                                               <  2:3969198/149‑1 (MQ=255)
                                    aCTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGagag                                                                                                            <  1:3822093/150‑1 (MQ=255)
                                    aCTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGagag                                                                                                            <  1:3039990/150‑1 (MQ=255)
                                    aCTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGagag                                                                                                            <  2:3927625/150‑1 (MQ=255)
                                     cTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCTCTCACCCTAACCCTCTCCCTGAGGGAGAgg                                                                                                           <  1:824404/150‑1 (MQ=255)
                                     cTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTGAGGGAGAgg                                                                                                           <  2:718820/150‑1 (MQ=255)
                                     cTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAgg                                                                                                           <  2:2634611/150‑1 (MQ=255)
                                     cTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAgg                                                                                                           <  1:719494/150‑1 (MQ=255)
                                     cTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAgg                                                                                                           <  1:744963/150‑1 (MQ=255)
                                     cTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAgg                                                                                                           <  2:1482086/150‑1 (MQ=255)
                                     cTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAgg                                                                                                           <  2:2889425/150‑1 (MQ=255)
                                      tGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAggg                                                                                                          <  2:2033824/150‑1 (MQ=255)
                                        gcgcGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGa                                                                                                        <  1:420548/150‑1 (MQ=255)
                                        gcgcGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGa                                                                                                        <  1:3441726/150‑1 (MQ=255)
                                        gcgcGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCACACCCAAACCCACACCCTCAGGGAGAGGGGa                                                                                                        >  1:4155238/1‑150 (MQ=255)
                                           cgAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTTCGCCCCCGCACCCAAAAACACTACCACACGGAGAGGGGGCCg                                                                                                     >  2:446406/1‑150 (MQ=255)
                                               aTCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCCCACCCAAAACCACACCCACAGGGAGAGGGGCCCCTTCg                                                                                                 >  1:1741975/1‑150 (MQ=255)
                                                  gATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCCCCCCCAAAACCACACCCGCAGGGCGAGGGGGCCGGTCGgcg                                                                                              >  1:3591756/1‑150 (MQ=255)
                                                  gATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCCCCCCCCAACCCACACCCACACGGGGGGGGGGCCCGTCGgcg                                                                                              >  2:3957439/1‑150 (MQ=255)
                                                  gATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCCCACCCCAACCCACGCCCGCCGGGCGCGGGGCCCGGTCGgcg                                                                                              >  2:1300824/1‑150 (MQ=255)
                                                    tCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCt                                                                                            <  1:503789/150‑1 (MQ=255)
                                                     cAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCtg                                                                                           <  2:950949/150‑1 (MQ=255)
                                                      aGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCCCACCCAAAACCGCACCCGCCGGGGGAGGGGGCCCGTCGgcggggg                                                                                          >  1:156937/1‑146 (MQ=255)
                                                       gCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCTTCAGGGAGAGGGGACCGTTCGGCGCtgta                                                                                         <  1:556311/150‑1 (MQ=255)
                                                        cGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCtgtat                                                                                        <  2:1214835/150‑1 (MQ=255)
                                                         gAAATTCCGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCtgtatg                                                                                       <  1:495851/150‑1 (MQ=255)
                                                         gAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCtgtatg                                                                                       <  1:1348172/150‑1 (MQ=255)
                                                           aaTTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCtgtatgta                                                                                     <  2:2145005/150‑1 (MQ=255)
                                                            aTTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTAc                                                                                    <  2:1242463/150‑1 (MQ=255)
                                                            aTTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTAc                                                                                    <  2:484617/150‑1 (MQ=255)
                                                             ttACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCCCCCCCAAAACCACAACCACACGGGGAGGGGACCCGTCGGCGCggtattttcg                                                                                   >  1:2502550/1‑145 (MQ=255)
                                                                cGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTccc                                                                                <  1:12300/150‑1 (MQ=255)
                                                                cGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTccc                                                                                <  1:2793725/150‑1 (MQ=255)
                                                                 gCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCCCACCCGAACCCACAACCGCAGGGCGGGGGGACCCTTCCGCGCGGGATTTACTccca                                                                               >  2:3429146/1‑149 (MQ=255)
                                                                 gCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCACACCCAAAACCACAACCACAGGGAGAGGGGACCCGTCGGCGCagtatgtaaaacca                                                                               >  1:2440811/1‑144 (MQ=255)
                                                                   aaGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCACACCCGAAACCACACCCCCAGGGGGAGGGGACCGTTAGGCGCTGGATGTACTACcaca                                                                             >  2:3505747/1‑150 (MQ=255)
                                                                        tCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCCCACCCCAAAACACAACCACACGGGGCGGGGGCCCTTCGGCGCGGTATTTACACCCacacacag                                                                        >  1:3238431/1‑146 (MQ=255)
                                                                          gCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGaa                                                                      <  2:3283735/150‑1 (MQ=255)
                                                                           cTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCCGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGaac                                                                     <  1:968701/150‑2 (MQ=255)
                                                                           cTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGaaa                                                                     <  2:2643622/150‑1 (MQ=255)
                                                                           cTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCGCCCCCAAACCCACACCCAAACGGCGAGGGGGCCCTTCGGCGCTGTATGGAAACCCACACACAGaaa                                                                     >  1:2282778/1‑150 (MQ=255)
                                                                                   ggggCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCg                                                             <  2:2208137/150‑1 (MQ=255)
                                                                                    gggCTGGGTAAAGTGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTACCCCTCTCCCTCAGGGAGTGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGc                                                            <  1:3098669/150‑1 (MQ=255)
                                                                                     ggCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTGGTTTGCGCCCCCTAAGCCTTAGCCTATGCCTGATGGTGTTGGGTCCGTTTGGGGCTGTTTTTAATTCCTAACTCTGTATAGACAACGCa                                                           >  2:2366658/1‑150 (MQ=255)
                                                                                      gCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCACACCCAAAACCCAAACCACACGGCGGGGGGGCCGGTCGGGGCGGTGTGTTCTCCCGCACACGGACAAGACAcccccc                                                          >  1:1289717/1‑146 (MQ=255)
                                                                                        tGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCGCACCCGAAACCACAACCGCAGGGAGAGGGGACCCGTAGGCGCGGTATTTAAACCCACAAACAGAAACCACACCCCAcac                                                        >  2:3759806/1‑148 (MQ=255)
                                                                                         gggTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCActct                                                       <  1:2684031/150‑1 (MQ=255)
                                                                                          ggTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCACACCCAAAACCACACCCTCAGGGAGAGGGGACCGTTCGGCGCGGTATGTACTCCCTCACTCAGCAACCCCACCGCACTCtt                                                      >  1:3811269/1‑150 (MQ=255)
                                                                                            tAAAGAGTTGCCTGCGGCACTGACTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCtttt                                                    <  2:2106061/150‑1 (MQ=255)
                                                                                             aaaGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCttttt                                                   <  1:2045363/150‑1 (MQ=255)
                                                                                                gagTTGCCTGCGGCACTGGCGCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTtc                                                <  2:2977012/150‑1 (MQ=255)
                                                                                                  gTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTtctc                                              <  1:2933147/150‑1 (MQ=255)
                                                                                                        tGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGc                                        <  2:2471116/150‑1 (MQ=255)
                                                                                                            gCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCTccccg                                    <  2:2130846/150‑2 (MQ=255)
                                                                                                                tGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCgg                                <  1:1413001/150‑1 (MQ=255)
                                                                                                                  gCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTTCGCCCCCACACCCCAAACCACACCCACAGGGGGAGGGGCCCCGTCGGCGCGGTTTTGACGCCCACACTCAGTAACGACACCCCACACGTTTTTTGTACCGCGCCCCACCgggg                              >  2:1978162/1‑150 (MQ=255)
                                                                                                                   cTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGga                             <  2:3403906/150‑1 (MQ=255)
                                                                                                                    tCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCGCACCCGAACCCACGCCCGCAGGGCGGGGGGACCCTTCGGCGCTGTGTGTACTCCCGCACACTGAAACGACACCCCACTCTTTTTTTCTCCCACGCCCCTCCggggcg                            >  2:2787153/1‑148 (MQ=255)
                                                                                                                    tCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCCCACCCAAAACCCCTCCCGCCGGGCGGGGGGGCCCGTGGGGGCGGGGTGTACTCCCGCCCCCGGAAACGACCACCCACACGTTTTTTTTCCCGCCCCCCACCcggggg                            >  2:3969319/1‑148 (MQ=255)
                                                                                                                    tCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCGCCCCCAAAACCACTCCCACAGGGCGCGGGGGCCGGTGGGCGCGGTGAGTAAGCCCGCACGCTGGAACCACACCCCCCACTTTTTTTAGACCACGCCCCCACggggcg                            >  2:3733434/1‑148 (MQ=255)
                                                                                                                     cccATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGCCACCGCACTCGTTTTTTCTCCCTCGCCCCTCCGGGgaga                           <  2:2559791/150‑1 (MQ=255)
                                                                                                                     cccATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGgaga                           <  1:1607172/150‑1 (MQ=255)
                                                                                                                      ccATGGCGAATGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGagag                          <  2:1119190/150‑1 (MQ=255)
                                                                                                                      ccATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGagag                          <  1:4112860/150‑1 (MQ=255)
                                                                                                                      ccATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGagag                          <  1:4112846/150‑1 (MQ=255)
                                                                                                                      ccATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCCCGGagag                          <  1:2533325/150‑1 (MQ=255)
                                                                                                                      ccATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCGCCCCCAAAACCACTCCCACACGGAGGGGGGACCCGTCGGCGCGGGATGTACACCCACCCGCGGGAACGACCCCCCACACGTTTTTTCTCCCGCGCCCCACCCGGGcgag                          >  2:3834435/1‑150 (MQ=255)
                                                                                                                         tGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCTCCCCGGAGGGGCGAggga                       <  2:1933144/150‑2 (MQ=255)
                                                                                                                             gAAAGGGAATGCGGCATAGTCGTTTGCGCCACCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGTGAGAGGGCCggg                   <  2:483978/150‑1 (MQ=255)
                                                                                                                                 gggAATGCGGCATAGTCGTTTGCGCCCCCGCCCCCGAACCCACACCCACCCGGCGAGGGGACCCGTCGGCGCCGGCTGGACGCCCCCACACGGAAACGACCCCCCCCGCGTTTGTTCTCCCGCCCCCCCCCCGGGCGAGGGCCggggggg               >  2:3778568/1‑147 (MQ=255)
                                                                                                                                  ggAATGCGGCATCGGCGTTGGCGCCCCCTCACCCTACCCCCCTCCCGCCGGGAGCGGGGACCGTTCGGCGCTGTATGGACTCCCTCACCCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAg              <  1:34332/150‑1 (MQ=255)
                                                                                                                                  ggAATGCGACATAGTCGTTTGCGCCCCCTCACCCTACCCCTCTCCCTCCGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAg              <  2:1443220/150‑1 (MQ=255)
                                                                                                                                   gAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAgg             <  2:157572/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
CTGATCGAAAAAGAGATGAAAGTGGCGATGACGCTGACTGGCGCGAAATCGATCAGCGAAATTACGCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCG  >  NC_000913/3780874‑3781165

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: