Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,178,247 A→C 5.1% T169P (ACC→CCC)  nusG → transcription termination factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,178,2470AC 5.1% 1076.5 / 36.9 296T169P (ACC→CCC) nusGtranscription termination factor
Reads supporting (aligned to +/- strand):  ref base A (162/119);  new base C (11/4);  total (173/123)
Fisher's exact test for biased strand distribution p-value = 2.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.54e-01

Only 100 of 297 total aligned reads displayed.

GTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTA  >  NC_000913/4178098‑4178395
                                                                                                                                                     |                                                                                                                                                    
 tGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGAc                                                                                                                                                     >  2:3399175/1‑150 (MQ=255)
        ccGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTa                                                                                                                                              <  2:960559/150‑1 (MQ=255)
         cgcgTCCGAAACCGCTGTTTGAACCGGGTGAACTGGTCCGTGTTACTGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTag                                                                                                                                             <  2:2397157/150‑1 (MQ=255)
         cgcgTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGTCTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTag                                                                                                                                             <  2:3151426/150‑1 (MQ=255)
             tCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCt                                                                                                                                         >  1:4162900/1‑150 (MQ=255)
              ccGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTg                                                                                                                                        <  1:1443672/150‑1 (MQ=255)
               cGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTgg                                                                                                                                       >  2:2564437/1‑150 (MQ=255)
               cGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTgg                                                                                                                                       >  2:11892/1‑150 (MQ=255)
               cGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTgg                                                                                                                                       >  2:3893521/1‑150 (MQ=255)
                   aaCGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACtt                                                                                                                                   <  2:3383529/150‑1 (MQ=255)
                      gCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAg                                                                                                                                >  2:3348421/1‑150 (MQ=255)
                       cTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGc                                                                                                                               >  2:1935226/1‑150 (MQ=255)
                       cTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGc                                                                                                                               <  2:3747701/150‑1 (MQ=255)
                        tGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGcc                                                                                                                              >  1:2651796/1‑150 (MQ=255)
                          tttGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAg                                                                                                                            >  2:3227621/1‑150 (MQ=255)
                           ttGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAgg                                                                                                                           >  2:2442238/1‑150 (MQ=255)
                           ttGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAgg                                                                                                                           >  1:665331/1‑150 (MQ=255)
                             gAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGtt                                                                                                                         >  1:1541587/1‑150 (MQ=255)
                             gAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGtt                                                                                                                         >  1:3809600/1‑150 (MQ=255)
                             gAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGtg                                                                                                                         >  1:2237456/1‑149 (MQ=255)
                             gAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTAGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGtt                                                                                                                         >  2:3955569/1‑150 (MQ=255)
                               aCCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGa                                                                                                                       >  1:4018386/1‑150 (MQ=255)
                                 cGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGaaa                                                                                                                     >  1:3280898/1‑150 (MQ=255)
                                  gggTGAAATGGTCCGTGTTAATGCTGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGaaaa                                                                                                                    <  1:112127/150‑1 (MQ=255)
                                  gggTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGaaaa                                                                                                                    <  2:2583520/150‑1 (MQ=255)
                                   ggTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGaaaaa                                                                                                                   >  2:1278890/1‑150 (MQ=255)
                                             tCCGTGTTAATGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCa                                                                                                         >  1:1019375/1‑150 (MQ=255)
                                             tCCGTGTTAATGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCa                                                                                                         >  1:3177758/1‑150 (MQ=255)
                                             tCCGTGTTAATGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCa                                                                                                         >  1:2361287/1‑150 (MQ=255)
                                               cGTGTTAATGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGc                                                                                                       <  1:2952693/150‑1 (MQ=255)
                                               cGTGATAATGATGGTCCCTTCGCTGACTACAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAACTGTCTGTTACTAACTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGc                                                                                                       <  2:3466002/150‑1 (MQ=255)
                                                  gtTAATGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGAt                                                                                                    >  1:2332811/1‑150 (MQ=255)
                                                  gtTAATGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGAt                                                                                                    >  2:3997301/1‑150 (MQ=255)
                                                   ttAATGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATc                                                                                                   >  1:752079/1‑150 (MQ=255)
                                                     aaTGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCaa                                                                                                 <  1:2773007/150‑1 (MQ=255)
                                                       tgatgGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCaaaa                                                                                               >  1:3655988/1‑150 (MQ=255)
                                                        gatgGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCaaaaa                                                                                              >  1:485550/1‑150 (MQ=255)
                                                        gatgGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCaaaaa                                                                                              >  1:3000822/1‑150 (MQ=255)
                                                        gatgGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCaaaaa                                                                                              >  2:3800212/1‑150 (MQ=255)
                                                          tgGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAg                                                                                            >  2:3668102/1‑150 (MQ=255)
                                                             tCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAgcgg                                                                                         <  2:2851349/150‑1 (MQ=255)
                                                              ccGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATTTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAgcggc                                                                                        <  2:383876/150‑1 (MQ=255)
                                                              ccGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAgcggc                                                                                        >  1:1662942/1‑150 (MQ=255)
                                                                gTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGa                                                                                      <  2:624867/150‑1 (MQ=255)
                                                                      tGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAAt                                                                                >  2:53724/1‑150 (MQ=255)
                                                                          ttCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGtt                                                                            >  1:1577724/1‑150 (MQ=255)
                                                                           taaccGGTTTTTTTGAAGAAGTGGTTTCCGATAATTCTCGTCTTAATGTTTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTTAAAAACCATAACCCCGCGATAAAAAAAGCGGCGATTTAATCGTTg                                                                           <  1:3967802/146‑1 (MQ=255)
                                                                           tCAACGGTGTTGTTGAAGATGTGTATTACTTTAAATCTCGTCTGATTTTGTCTTTTTCTATCTTGGGTCGTGCGACCCGGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCAAGCGATCAAAAAAGCGGCGATTTAATCGTTg                                                                           <  1:3567873/150‑1 (MQ=255)
                                                                             aaCGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGCCCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCAGCGATTTAATCGTTGca                                                                         >  1:3765176/1‑150 (MQ=255)
                                                                              agggTGTTGTTTCAGACGTGGATTACGAGAAATCTCGTCTGAATGTTTCTTTTTCTATCTGCGGTCGTGCGACCCCGGTCGAGCTGGACTACAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGcac                                                                        <  1:2020630/148‑1 (MQ=255)
                                                                              agggTGTTGTTTAAGATGTGTATTACGATAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGTGCTGGACTTCAGCCAGTTTTAAAAAGCCTAACCCACCGATCAAAAAAGCGGCGATTTAATCGTTGcac                                                                        <  1:3540833/148‑1 (MQ=255)
                                                                                ggTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACaa                                                                      >  2:1066028/1‑150 (MQ=255)
                                                                                  tgttgtTGAAGAAGTGTATTACGAGAAATCTCGTCTGAATGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGTACTTCAGCCTGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAgg                                                                    <  1:119992/150‑1 (MQ=255)
                                                                                  tgttgtTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAgg                                                                    >  2:2159473/1‑150 (MQ=255)
                                                                                    ttgttgAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGCCCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCg                                                                  >  2:2988627/1‑150 (MQ=255)
                                                                                     tgttgAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGt                                                                 <  2:463321/150‑1 (MQ=255)
                                                                                      gttgAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTg                                                                >  2:118114/1‑150 (MQ=255)
                                                                                      gttgAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTg                                                                >  1:3432871/1‑150 (MQ=255)
                                                                                          aagaagTGTATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCCGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGAt                                                            <  1:2574247/150‑1 (MQ=255)
                                                                                          aagaagTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGAt                                                            <  1:2178858/150‑1 (MQ=255)
                                                                                          aagaagTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGAt                                                            >  1:1512592/1‑150 (MQ=255)
                                                                                            gaagTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCCGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTg                                                          <  1:1868160/150‑1 (MQ=255)
                                                                                            gaagTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTg                                                          <  2:4049535/150‑1 (MQ=255)
                                                                                            gaagTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTg                                                          >  1:550089/1‑150 (MQ=255)
                                                                                            gaagTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTg                                                          <  2:2733105/150‑1 (MQ=255)
                                                                                                   aTTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACa                                                   >  1:2528397/1‑150 (MQ=255)
                                                                                                    ttACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACaa                                                  <  1:3254592/150‑1 (MQ=255)
                                                                                                    ttACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACaa                                                  <  2:3260942/150‑1 (MQ=255)
                                                                                                        gagaAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTc                                              <  1:318875/150‑1 (MQ=255)
                                                                                                         agaAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTcg                                             <  1:1292829/150‑1 (MQ=255)
                                                                                                                tcGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCttt                                      >  1:3752606/1‑150 (MQ=255)
                                                                                                                  gTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCCGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTg                                    >  1:891425/1‑150 (MQ=255)
                                                                                                                  gTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCCGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTg                                    >  2:2008968/1‑150 (MQ=255)
                                                                                                                   tCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGt                                   >  1:2303781/1‑150 (MQ=255)
                                                                                                                     tGAATGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGttt                                 <  1:2827548/150‑1 (MQ=255)
                                                                                                                      gAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGtttt                                >  1:383003/1‑150 (MQ=255)
                                                                                                                      gAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGtttt                                >  2:1595222/1‑150 (MQ=255)
                                                                                                                       aaaGTGTCTGTTTCTATCTTCGGTCGTGCGCCCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGttttt                               >  2:2064051/1‑150 (MQ=255)
                                                                                                                        aaGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTa                              <  1:461780/150‑1 (MQ=255)
                                                                                                                         aGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAt                             <  2:3538007/150‑1 (MQ=255)
                                                                                                                         aGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTAt                             <  2:1551192/150‑1 (MQ=255)
                                                                                                                             tCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGc                         <  2:3013173/150‑1 (MQ=255)
                                                                                                                               tGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCt                       >  1:2605131/1‑150 (MQ=255)
                                                                                                                                 tttCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTg                     >  1:1001857/1‑150 (MQ=255)
                                                                                                                                 tttCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTg                     >  2:2286496/1‑150 (MQ=255)
                                                                                                                                 tttCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTg                     >  2:2659821/1‑150 (MQ=255)
                                                                                                                                 tttCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTg                     >  2:2327826/1‑150 (MQ=255)
                                                                                                                                     tATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGTTTGGAATACAATTTCGCGCGTTTTGTTTTTATGGGGCTTGCCCg                 >  2:4152611/1‑150 (MQ=255)
                                                                                                                                         ttCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTaaa             >  1:1781701/1‑150 (MQ=255)
                                                                                                                                         ttCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTaaa             <  1:3893521/150‑1 (MQ=255)
                                                                                                                                           cGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAAc           <  1:1824985/150‑1 (MQ=255)
                                                                                                                                             gTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGa         >  2:3080326/1‑150 (MQ=255)
                                                                                                                                             gTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGa         >  2:2624319/1‑150 (MQ=255)
                                                                                                                                             gTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGa         <  2:4181960/150‑1 (MQ=255)
                                                                                                                                             gTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGa         <  1:3066428/150‑1 (MQ=255)
                                                                                                                                                 tGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGAtttt     >  1:4046584/1‑150 (MQ=255)
                                                                                                                                                 tGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGAtttt     >  1:3131279/1‑150 (MQ=255)
                                                                                                                                                  gCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGAttttt    <  1:127170/150‑1 (MQ=255)
                                                                                                                                                    gACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTta  <  1:2442238/150‑1 (MQ=255)
                                                                                                                                                    gACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTta  <  2:769839/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GTGATAAGCCGCGTCCGAAAACGCTGTTTGAACCGGGTGAAATGGTCCGTGTTAATGATGGTCCGTTCGCTGACTTCAACGGTGTTGTTGAAGAAGTGGATTACGAGAAATCTCGTCTGAAAGTGTCTGTTTCTATCTTCGGTCGTGCGACCCCGGTAGAGCTGGACTTCAGCCAGGTTGAAAAAGCCTAACCCAGCGATCAAAAAAGCGGCGATTTAATCGTTGCACAAGGCGTGAGATTGGAATACAATTTCGCGCCTTTTGTTTTTATGGGCCTTGCCCGTAAAACGATTTTTTA  >  NC_000913/4178098‑4178395

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: