Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,326,047 C→T 6.2% intergenic (‑306/+352) yjdN ← / ← yjdM metalloprotein superfamily protein/zinc‑ribbon family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,326,0470CT 6.2% 496.9 / 16.4 178intergenic (‑306/+352)yjdN/yjdMmetalloprotein superfamily protein/zinc‑ribbon family protein
Reads supporting (aligned to +/- strand):  ref base C (72/93);  new base T (4/7);  total (76/102)
Fisher's exact test for biased strand distribution p-value = 7.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 179 total aligned reads displayed.

AGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGG  >  NC_000913/4325904‑4326195
                                                                                                                                               |                                                                                                                                                    
 ggCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCg                                                                                                                                               <  1:1342772/150‑1 (MQ=37)
   ccTGAGAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAACCGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGa                                                                                                                                             <  2:2040520/150‑1 (MQ=255)
     tGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATg                                                                                                                                           >  1:3049913/1‑150 (MQ=37)
       attaGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATCGCCGGATGCGGCGTAAACGCCTTTTCCGGCCTCCGGAGGGTGCGGGATTTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATgcg                                                                                                                                         <  1:3829276/147‑1 (MQ=11)
       agaaGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATgcg                                                                                                                                         <  2:563441/148‑1 (MQ=37)
       aTAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATgcg                                                                                                                                         <  2:1306561/150‑1 (MQ=37)
       aTAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATgcg                                                                                                                                         <  2:3921928/150‑1 (MQ=37)
       aTAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAAGTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGGTGCCGGATgcg                                                                                                                                         >  2:2140432/1‑150 (MQ=255)
         aaGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATgcggc                                                                                                                                       >  1:2295338/1‑150 (MQ=37)
          aGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATgcggcg                                                                                                                                      <  2:3351863/150‑1 (MQ=37)
           gACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATgcggcgg                                                                                                                                     >  2:4187995/1‑149 (MQ=37)
           gACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGt                                                                                                                                     >  2:970087/1‑150 (MQ=37)
           gACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGt                                                                                                                                     >  1:3511749/1‑150 (MQ=37)
           gACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGGAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGt                                                                                                                                     >  1:1385441/1‑150 (MQ=37)
              gcgcAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTaaa                                                                                                                                  >  2:3531166/1‑150 (MQ=37)
                 cAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGc                                                                                                                               <  2:1972226/150‑1 (MQ=37)
                 cAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGc                                                                                                                               >  2:3675042/1‑150 (MQ=37)
                  aaGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGcc                                                                                                                              <  2:3383292/150‑1 (MQ=37)
                         gCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCg                                                                                                                       <  1:733713/150‑1 (MQ=37)
                           aTCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGc                                                                                                                     >  2:4200501/1‑150 (MQ=37)
                              aGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTa                                                                                                                  >  2:3123391/1‑150 (MQ=37)
                               ggCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTAc                                                                                                                 >  2:2939669/1‑150 (MQ=37)
                                  aGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGa                                                                                                              >  2:4060923/1‑150 (MQ=37)
                                   gTCGGCACCCGGGCCGGATGCGGCGTACCCGCCGTATCCGGCCTCCGGAGGGTGCGGGAATGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAt                                                                                                             <  2:1279336/150‑1 (MQ=11)
                                       gCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGGATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCATATCCGGc                                                                                                                     >  1:1859124/1‑138 (MQ=21)
                                       gCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGc                                                                                                                     >  1:3929101/1‑138 (MQ=34)
                                       gCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGAAGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTg                                                                                                         >  1:10411/1‑150 (MQ=37)
                                       gCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATGTGTAGGCCTGATAAGACGCGCACGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTg                                                                                                         >  2:3983708/1‑150 (MQ=12)
                                           cATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTAGGCACCGTTGCCGGATGCGGCGAAAACGCCTTATCCGGCGTACCGATGGTGCggg                                                                                                     >  2:3532628/1‑150 (MQ=255)
                                                       cggcgTTAACGCCTTCTGCGGCCTTCGGAGGGTGCGGGTCTTTGGAGGCCTGATAAGACGCGCTAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGcc                                                                                         <  2:1433719/150‑1 (MQ=11)
                                                       cggcgTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGcc                                                                                         <  1:3158312/150‑1 (MQ=32)
                                                        ggcgTACACGCCTTATCCGGCCTACGGAGGGTGCGGGACTGTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCTGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCt                                                                                        <  1:1449908/150‑1 (MQ=255)
                                                          cgTAAACGCCTTATCCTGCCTACGGAGGGTGCGGTAATTTGTAGGCCTGATAAGACGCGCTAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGa                                                                                      <  2:1741931/150‑1 (MQ=12)
                                                           gTAAACGCCTTATGCGGCCTTCGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGAt                                                                                     <  2:2448992/150‑1 (MQ=12)
                                                           gTAAACGCCTTATCCTGCCCTCGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGAt                                                                                     <  2:2449210/150‑1 (MQ=12)
                                                              aaCGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCTTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGagcag                                                                                  >  1:3880771/1‑146 (MQ=17)
                                                                      aTCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCaa                                                                          <  2:3070854/150‑1 (MQ=32)
                                                                       tCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAg                                                                         >  2:4035337/1‑150 (MQ=32)
                                                                        ccGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGc                                                                        <  2:1087905/150‑1 (MQ=32)
                                                                             ccgcGGAGGGTGCGGGTCTGTGGAGGCCTGATAAGCCGCGCCAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGc                                                                   <  2:159878/147‑1 (MQ=11)
                                                                             cTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGc                                                                   >  2:2697354/1‑150 (MQ=32)
                                                                             cTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGc                                                                   <  1:4200501/150‑1 (MQ=32)
                                                                                 ggAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCa                                                               <  1:93487/150‑1 (MQ=32)
                                                                                 ggAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCa                                                               <  2:2295338/150‑1 (MQ=32)
                                                                                 ggAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCa                                                               <  2:1398141/150‑1 (MQ=32)
                                                                                     ggTGCGGGTCTTTGGAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGGTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCa                                                           <  2:3342120/150‑1 (MQ=255)
                                                                                     ggTGCGGGAATTTGTCGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCa                                                           <  2:3301711/150‑1 (MQ=17)
                                                                                     gctGCGGGACCTCGTCGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAGTCGGCACAGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCACGCGTCGCATCAGGCa                                                           <  2:3342085/148‑1 (MQ=11)
                                                                                      gTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAg                                                          >  1:2446914/1‑150 (MQ=32)
                                                                                         cGGGATTTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTg                                                       <  2:1796279/150‑1 (MQ=25)
                                                                                         cGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCatatg                                                       >  2:742615/1‑146 (MQ=14)
                                                                                         cGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTg                                                       >  1:2200120/1‑150 (MQ=35)
                                                                                         cGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTg                                                       <  1:2522858/150‑1 (MQ=35)
                                                                                          gggAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCgg                                                                                                      >  1:1071861/1‑102 (MQ=32)
                                                                                           ggAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGc                                                     >  2:4161599/1‑150 (MQ=17)
                                                                                            gAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTGCGGAGGGGGGGGGAAGTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCa                                                    >  1:2816938/1‑150 (MQ=11)
                                                                                            gAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGAATCAGGCAGAAGGCa                                                    >  1:2671137/1‑150 (MQ=12)
                                                                                            gAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGGAAACGCCTTATCCGGCCTACCGATGCAGCGGGAAGGACTAGTCCTTAAAAGACGCGCCAGCGTCGCATCACGCacaaggca                                                    >  1:1238138/1‑143 (MQ=11)
                                                                                                 tGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAGCGCCTTATCCGGCCTCCGGATGGTGCGGGAAATTGTAGGCCTGATAAGACGCGCACGCGTCGCATCAGGCAGATGGCACAGtt                                               >  2:3746168/1‑150 (MQ=11)
                                                                                                 tGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGtt                                               <  1:3675042/150‑1 (MQ=39)
                                                                                                 tGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGtt                                               >  2:2869779/1‑150 (MQ=39)
                                                                                                 tGTAGGCCAGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGtt                                               <  1:2057502/150‑1 (MQ=255)
                                                                                                   tAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGAAAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGc                                             >  2:736003/1‑150 (MQ=255)
                                                                                                    aGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGcc                                            <  1:3882794/150‑1 (MQ=39)
                                                                                                       ccTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGa                                         <  1:3903769/150‑1 (MQ=39)
                                                                                                       ccTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGATGGCACAGTTGCCGGa                                         >  2:3750582/1‑150 (MQ=21)
                                                                                                        cTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTTGGCCTGATAAGACGCGCAAGCGGCGCATCAGGCAGTTGGCACAGTTGCCGGAt                                        >  1:1713929/1‑150 (MQ=255)
                                                                                                         tGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGACTTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATg                                       <  1:571682/150‑1 (MQ=255)
                                                                                                         tGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATg                                       <  1:970205/150‑1 (MQ=39)
                                                                                                         tGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATg                                       <  1:4187995/150‑1 (MQ=39)
                                                                                                           aTAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGACTGAAAAGACGCGCAAGCGTCGCATCAGGCAGATGGCACTGTTGCCGGATgcg                                     >  2:459218/1‑150 (MQ=255)
                                                                                                           aTAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACCGATGGTGCGGCAATGTGTTGGACTGATAAGACGCGCAAGCGTCGCATCAGGCAGATGGCACAGGTGCCGGATgcg                                     >  2:692684/1‑150 (MQ=11)
                                                                                                            tAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATgcgg                                    <  1:1048152/150‑1 (MQ=39)
                                                                                                             ccgACGCGCCCGCGTCGCCTCAGGCAGTCGGCCCCGTGGCCGGATGCGGCGTCCACGCCTGATCCGGCCTACGGATGGTGCGGGAATTTGGAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATgcggc                                   <  2:3971147/148‑1 (MQ=14)
                                                                                                             aaGACGCTCAAGCGTCGTATCAGGCAGTCTGCTCCGTTGCCTGATGCGGCGTTAACGCCTTATCCTGCCTACTGATGGTGCTGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATAAGGCAGTTGGCACTGTTGCCGGATgcggc                                   <  2:3875043/150‑1 (MQ=14)
                                                                                                                aCGCGCAAGCGTCGCATCAGGCATAAGGCACCGTTGCCGGATGCGGCGGGAACGCCTTATCCGGCCTACCGATGGAGCGGGAATTAGTAGTCCTGATCAGACGCGCAAGAGACGCAACAGGCAGAAGGCACCGATGCCGTATgagccgag                                >  2:2873474/1‑143 (MQ=2)
                                                                                                                aCGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGATGGCACTGTTGCCGGATGCGGCGTa                                >  2:1893799/1‑150 (MQ=38)
                                                                                                                  gcgcGAGCGTCGCATCAGGCAGTCGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTaaa                              >  1:3723051/1‑150 (MQ=21)
                                                                                                                  gcgcAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGGTGCGGCGTAAACGCCGTATCCGGCCAACGGATGGTGCGGGACTTTTTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACGGTTGCCGGATGCGGCGTaaa                              >  1:3274048/1‑150 (MQ=11)
                                                                                                                      aaGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATTCGGCGTAAACGCCTTACCCGGCCTACGGATGGTTCGGGAATTTTTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGcc                          <  2:177728/150‑1 (MQ=255)
                                                                                                                       aGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCt                         <  2:10411/150‑1 (MQ=39)
                                                                                                                           tCGCATCAGGCAGTCGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATc                     >  2:1726262/1‑150 (MQ=17)
                                                                                                                            cGCATCAGGCAGTCGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATcc                    <  1:1726262/150‑1 (MQ=37)
                                                                                                                            cGCATCAGGCAGTCGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATcc                    <  1:743568/150‑1 (MQ=17)
                                                                                                                             gcttCAGGCAGTCGGCACCGGTGCCGGTATCGGCGTAAAAGCCTTATCCCGCCTACGGATGGTGCGGGAATTTGTTGGCCTGTTAAGACGCGCAAGCGTCGCATCAGGCAGTTGTCACTGTTGCCGTATGCGGCGTAAACGCCTTATCCg                   <  1:2782938/147‑1 (MQ=14)
                                                                                                                              cATCAGGCAGTCGGCACTGTTGCCGGATGCGGCGTAAACGCGTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCgg                  <  2:1160775/150‑1 (MQ=255)
                                                                                                                              cATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCgg                  <  2:3419289/150‑1 (MQ=39)
                                                                                                                                      aGTCGGCACTGTTGCCGGATGCGGCGTAACCGCCTTCTCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGa          <  2:2988319/150‑1 (MQ=255)
                                                                                                                                      aGTCGGCACCGTTGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGa          <  2:768486/150‑1 (MQ=255)
                                                                                                                                       gTCGGCTCTGTTGACGGTTGCGGCGTTAAAGTATTTTCCTGCCCTCGTATGGTGCTGGTTTTTGTAGGCCTGATAAGACGCGCTAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAg         <  2:1844373/150‑1 (MQ=11)
                                                                                                                                       gTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTTTCCGGCCTTCGGATGGTGCGGGATTTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAg         <  2:3572549/150‑1 (MQ=255)
                                                                                                                                       gTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATTAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAg         <  1:4179798/150‑1 (MQ=255)
                                                                                                                                       gTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAg         <  1:4179794/150‑1 (MQ=40)
                                                                                                                                       gTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAg         <  1:1828715/150‑1 (MQ=40)
                                                                                                                                        tCGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGTCGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAgg        <  1:3120844/150‑1 (MQ=9)
                                                                                                                                        tCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCATATGGCACAGTTGCCGGATGCGGCGGAATCGCCTTATCCGGCCTACCGAgg        >  2:1773832/1‑150 (MQ=11)
                                                                                                                                        tCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAgg        <  1:4035261/150‑1 (MQ=40)
                                                                                                                                          ggCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACAGTTGCCGGATGCGGCGAAATCGCCTTATCCGGCCTACGGAGGGt      >  2:3961446/1‑150 (MQ=255)
                                                                                                                                            cACCGTTGCCGGATGCGGCGTAAACGCCATATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGc    >  2:836072/1‑150 (MQ=21)
                                                                                                                                              ccGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCgg  <  1:3415371/150‑1 (MQ=38)
                                                                                                                                               |                                                                                                                                                    
AGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGG  >  NC_000913/4325904‑4326195

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: