Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,326,088 T→G 5.5% intergenic (‑347/+311) yjdN ← / ← yjdM metalloprotein superfamily protein/zinc‑ribbon family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,326,0880TG 5.5% 457.0 / 10.5 173intergenic (‑347/+311)yjdN/yjdMmetalloprotein superfamily protein/zinc‑ribbon family protein
Reads supporting (aligned to +/- strand):  ref base T (69/92);  new base G (8/3);  total (78/95)
Fisher's exact test for biased strand distribution p-value = 6.53e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 176 total aligned reads displayed.

GTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCA  >  NC_000913/4325939‑4326234
                                                                                                                                                     |                                                                                                                                                  
gTCGGCACCCGGGCCGGATGCGGCGTACCCGCCGTATCCGGCCTCCGGAGGGTGCGGGAATGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAt                                                                                                                                                    <  2:1279336/150‑1 (MQ=11)
gTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAt                                                                                                                                                    <  2:3687668/150‑1 (MQ=38)
gTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAt                                                                                                                                                    <  2:1783239/150‑1 (MQ=38)
 tCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATg                                                                                                                                                   <  1:2702213/150‑1 (MQ=38)
    gCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGGCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTg                                                                                                                                                >  2:3006320/1‑150 (MQ=17)
    gCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGAAGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTg                                                                                                                                                >  1:10411/1‑150 (MQ=37)
    gCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATGTGTAGGCCTGATAAGACGCGCACGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTg                                                                                                                                                >  2:3983708/1‑150 (MQ=12)
        cATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTAGGCACCGTTGCCGGATGCGGCGAAAACGCCTTATCCGGCGTACCGATGGTGCggg                                                                                                                                            >  2:3532628/1‑150 (MQ=255)
                gATGAGGCGTTACCGCCTTATCCTGCCATAGGAGGGTTCTGGATTTTGGAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTa                                                                                                                                    <  2:734516/150‑1 (MQ=11)
                   gcggcgTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGGAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTAGCCGGCCTACGGATGGTGCGGGAATTTGTAGGc                                                                                                                                 <  2:2675661/150‑1 (MQ=17)
                    cggcgTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGcc                                                                                                                                <  2:1700515/150‑1 (MQ=32)
                     ggcgTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCt                                                                                                                               <  1:1449798/150‑1 (MQ=32)
                       cgTAAACGCCTTATCCTGCCTACGGAGGGTGCGGTAATTTGTAGGCCTGATAAGACGCGCTAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGa                                                                                                                             <  2:1741931/150‑1 (MQ=12)
                        gTAAACGCCTTATGCGGCCTTCGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGAt                                                                                                                            <  2:2448992/150‑1 (MQ=12)
                           aaCGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCTTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGagcag                                                                                                                         >  1:3880771/1‑146 (MQ=17)
                              gCCTTATCCGGCCTTCTGAGGGTGCTGGAATTTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGAcg                                                                                                                      <  2:2113147/150‑1 (MQ=255)
                                   aTCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCaa                                                                                                                 <  2:3070854/150‑1 (MQ=32)
                                    tCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAg                                                                                                                >  2:4035337/1‑150 (MQ=32)
                                     ccGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGc                                                                                                               <  2:1087905/150‑1 (MQ=32)
                                      cGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGAAAACGCCTTATCCGGCCTACGGATGGAGCGGGAATATGTAGTCCTGATAAGACTCGCAAGCg                                                                                                              >  1:3240568/1‑150 (MQ=11)
                                         ccTCCGGAGGGAGCGGGACTTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACAGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCg                                                                                                           <  2:473809/150‑1 (MQ=255)
                                          cttcGGAGGGTGCGGGTCTGTGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGc                                                                                                          <  2:3273282/147‑1 (MQ=11)
                                          ccgcGGAGGGTGCGGGTCTGTGGAGGCCTGATAAGCCGCGCCAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGc                                                                                                          <  2:159878/147‑1 (MQ=11)
                                          cTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCTGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGc                                                                                                          <  1:419297/150‑1 (MQ=17)
                                          cTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGc                                                                                                          <  1:4200501/150‑1 (MQ=32)
                                          cTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGc                                                                                                          >  2:2697354/1‑150 (MQ=32)
                                              ggAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCa                                                                                                      <  2:1398141/150‑1 (MQ=32)
                                              ggAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCa                                                                                                      <  1:1650715/150‑1 (MQ=32)
                                              ggAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCa                                                                                                      <  2:2295338/150‑1 (MQ=32)
                                              ggAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCa                                                                                                      <  1:93487/150‑1 (MQ=32)
                                               gAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTCAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAg                                                                                                     <  2:1738771/150‑1 (MQ=17)
                                                  ggTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCa                                                                                                  <  2:3342184/150‑1 (MQ=32)
                                                  ggTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCa                                                                                                  >  2:3566356/1‑150 (MQ=32)
                                                  gctGCGGGACCTCGTCGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAGTCGGCACAGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCACGCGTCGCATCAGGCa                                                                                                  <  2:3342085/148‑1 (MQ=11)
                                                   gTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAg                                                                                                 >  1:2446914/1‑150 (MQ=32)
                                                      cGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCatatg                                                                                              >  2:742615/1‑146 (MQ=14)
                                                      cGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTg                                                                                              >  1:2200120/1‑150 (MQ=35)
                                                       gggAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCgg                                                                                                                                             >  1:1071861/1‑102 (MQ=32)
                                                         gAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTGCGGAGGGGGGGGGAAGTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCa                                                                                           >  1:2816938/1‑150 (MQ=11)
                                                         gAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATa                                                                                                                           >  2:2315856/1‑118 (MQ=32)
                                                              tGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAGCGCCTTATCCGGCCTCCGGATGGTGCGGGAAATTGTAGGCCTGATAAGACGCGCACGCGTCGCATCAGGCAGATGGCACAGtt                                                                                      >  2:3746168/1‑150 (MQ=11)
                                                              tGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGtt                                                                                      >  2:2869779/1‑150 (MQ=39)
                                                              tGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGtt                                                                                      <  1:3675042/150‑1 (MQ=39)
                                                                tAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGc                                                                                    <  1:1984488/150‑1 (MQ=255)
                                                                 aGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGcc                                                                                   <  1:3882831/150‑1 (MQ=39)
                                                                    ccTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGa                                                                                <  1:3903774/150‑1 (MQ=39)
                                                                     cTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGTCCTACGGATGGTGCGGGTATTTGTAGGCCTGATAAGACGCGCACGCGTCGCATCAGGCAGGTGGCACTGTTGCCggct                                                                               >  1:3403646/1‑148 (MQ=255)
                                                                     cTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCGTATCCGGCCTACGGATGGTGCGGGACTTTGTAGGCCTGCTAAGACGCGCAAGCGTCGCAGCAGGCCGTTGGCACTGGTGCCGGag                                                                               >  1:2631197/1‑149 (MQ=14)
                                                                      tGATAAGTCGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGTATTTGTGGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATg                                                                              <  1:4187884/150‑1 (MQ=255)
                                                                      tGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGACTTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATg                                                                              <  1:571682/150‑1 (MQ=255)
                                                                      tGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATg                                                                              <  1:970205/150‑1 (MQ=39)
                                                                      tGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATg                                                                              <  2:1385441/150‑1 (MQ=39)
                                                                        aTAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACCGATGGTGCGGCAATGTGTTGGACTGATAAGACGCGCAAGCGTCGCATCAGGCAGATGGCACAGGTGCCGGATgcg                                                                            >  2:692684/1‑150 (MQ=11)
                                                                          aaGACGCGCCAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATgcggc                                                                          <  2:3971131/150‑1 (MQ=255)
                                                                             aCGCGCAAGCGTCGCATCAGGCATAAGGCACCGTTGCCGGATGCGGCGGGAACGCCTTATCCGGCCTACCGATGGAGCGGGAATTAGTAGTCCTGATCAGACGCGCAAGAGACGCAACAGGCAGAAGGCACCGATGCCGTATgagccgag                                                                       >  2:2873474/1‑143 (MQ=2)
                                                                             aCGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGATGGCACTGTTGCCGGATGCGGCGTa                                                                       >  2:1893799/1‑150 (MQ=38)
                                                                               gcgcAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGGTGCGGCGTAAACGCCGTATCCGGCCAACGGATGGTGCGGGACTTTTTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACGGTTGCCGGATGCGGCGTaaa                                                                     >  1:3274048/1‑150 (MQ=11)
                                                                                  cAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGc                                                                  >  2:455424/1‑150 (MQ=39)
                                                                                    aGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCt                                                                <  2:10411/150‑1 (MQ=39)
                                                                                         cGCATCAGGCAGTCGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATcc                                                           <  1:1726262/150‑1 (MQ=37)
                                                                                         cGCATCAGGCAGTCGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATcc                                                           <  1:743568/150‑1 (MQ=17)
                                                                                          gcttCAGGCAGTCGGCACCGGTGCCGGTATCGGCGTAAAAGCCTTATCCCGCCTACGGATGGTGCGGGAATTTGTTGGCCTGTTAAGACGCGCAAGCGTCGCATCAGGCAGTTGTCACTGTTGCCGTATGCGGCGTAAACGCCTTATCCg                                                          <  1:2782938/147‑1 (MQ=14)
                                                                                          gCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCg                                                          >  2:3332127/1‑150 (MQ=39)
                                                                                           cATCAGGCAGTCGGCACTGTTGCCGGATGCGGCGTAAACGCGTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCgg                                                         <  2:1160775/150‑1 (MQ=255)
                                                                                           cATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCgg                                                         <  2:3419289/150‑1 (MQ=39)
                                                                                                   aGTCGGCACCGTTGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGa                                                 <  2:768486/150‑1 (MQ=255)
                                                                                                    gTCGGCTCTGTTGACGGTTGCGGCGTTAAAGTATTTTCCTGCCCTCGTATGGTGCTGGTTTTTGTAGGCCTGATAAGACGCGCTAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAg                                                <  2:1844373/150‑1 (MQ=11)
                                                                                                    gTCGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAg                                                <  1:3844627/150‑1 (MQ=37)
                                                                                                    gTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAg                                                <  1:4179794/150‑1 (MQ=40)
                                                                                                     tCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCATATGGCACAGTTGCCGGATGCGGCGGAATCGCCTTATCCGGCCTACCGAgg                                               >  2:1773832/1‑150 (MQ=11)
                                                                                                     tCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAgg                                               <  1:4035261/150‑1 (MQ=40)
                                                                                                       ggCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACAGTTGCCGGATGCGGCGAAATCGCCTTATCCGGCCTACGGAGGGt                                             >  2:3961446/1‑150 (MQ=255)
                                                                                                         cACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGc                                           <  1:3154554/150‑1 (MQ=39)
                                                                                                         cACCGTTGCCGGATGCGGCGTAAACGCCATATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGc                                           >  2:836072/1‑150 (MQ=21)
                                                                                                          actgTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCg                                          <  2:3230012/147‑1 (MQ=1)
                                                                                                              ttGCCGGATGCGGCGTAAACGCCATATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGaa                                      >  1:1438141/1‑150 (MQ=37)
                                                                                                                  cGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGACTTTg                                  >  2:3826248/1‑150 (MQ=37)
                                                                                                                  cGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTg                                  <  1:3531166/150‑1 (MQ=17)
                                                                                                                    gATGCGGCGTATACGCCTTACCGGGCCTTCGGATGGTGCGGGACTTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTa                                <  2:3924713/150‑1 (MQ=11)
                                                                                                                     aTGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAg                               <  1:2571992/150‑1 (MQ=37)
                                                                                                                       gcggcgTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGGCATCGCCTTATCCGGCCGACGGAGGGGGCGGGAATGTGTAGGc                             >  2:4079647/1‑150 (MQ=11)
                                                                                                                          gcgTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGttgtcatg                          >  1:2269931/1‑143 (MQ=255)
                                                                                                                          gcgTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTCAACGCCTTATCCGGCCTACGGAGGGTGCGGGACTTTGTAGGCCTg                          >  1:897596/1‑150 (MQ=255)
                                                                                                                          gcgTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTGTCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTg                          >  1:1089763/1‑150 (MQ=37)
                                                                                                                          gcgTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATc                                                            >  2:3679270/1‑116 (MQ=39)
                                                                                                                           cgTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGa                         <  2:281623/150‑1 (MQ=37)
                                                                                                                            gTAAACGCCTTATCCGGCCTACGGAAGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGAt                        >  2:2649479/1‑150 (MQ=21)
                                                                                                                             tAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATa                       >  2:2237507/1‑150 (MQ=37)
                                                                                                                               aaCGCCTTATCCGGCCTACGGATGGTGCGGGAATGTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGGTGGCACGGTTGCCGGATGCGGCGGAAACGCCTTATCCGGCCGACGGAGGGGGCGGGACGGTGAAGGCCGGATAAg                     >  2:2779411/1‑150 (MQ=11)
                                                                                                                                   ccTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGAcgc                 <  2:3723051/150‑1 (MQ=37)
                                                                                                                                     ttATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACAGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGAGCGGGAATGAGTAGGCCTGATCAGAcgctc               >  2:2717719/1‑148 (MQ=11)
                                                                                                                                      tATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCa              >  1:3982921/1‑150 (MQ=37)
                                                                                                                                            gCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCTGGCAGTGGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTc        <  1:1836366/150‑1 (MQ=21)
                                                                                                                                            gCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTc        <  1:1836422/150‑1 (MQ=37)
                                                                                                                                            gCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTc        <  2:3049874/150‑1 (MQ=17)
                                                                                                                                               tACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACAGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGGGCGGGAATGTGTAGGCCTGATAAGACGCGCAAGCGTCGCa     >  2:4137131/1‑150 (MQ=255)
                                                                                                                                                 cGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCACGCGTCGCATc   >  2:2974712/1‑150 (MQ=37)
                                                                                                                                                 cGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATc   <  2:1438141/150‑1 (MQ=37)
                                                                                                                                                 cGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATc   >  2:1005803/1‑150 (MQ=37)
                                                                                                                                                  ggATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGAGCGGGAATATGTAGTCCTGATAAGACGCGCAATCGTCGCATCa  >  1:1002049/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
GTCGGCACCATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTCGGCACCGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAGTTGGCACTGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAGGGTGCGGGAATTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCA  >  NC_000913/4325939‑4326234

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: