BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	423,539	T→G	6.3%	L341R (CTG→CGG)	malZ →	maltodextrin glucosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	423,539	0	T	G	6.3%	698.8 / 9.9	254	L341R (CTG→CGG)	malZ	maltodextrin glucosidase
Reads supporting (aligned to +/- strand): ref base T (115/118); new base G (2/19); total (117/137)
Fisher's exact test for biased strand distribution p-value = 3.67e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 257 total aligned reads displayed.

CTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCGATGTGGAAGATGCCGCC  >  NC_000913/423390‑423687

cTGGCTTGGCTATGCCAGCTTGCCGAATCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGCGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCt                                                                                                                                                      <  1:2686262/150‑1 (MQ=255)

cTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCt                                                                                                                                                      <  1:2936390/150‑1 (MQ=255)

cTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTG‑AAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTg                                                                                                                                                     <  1:1394713/150‑1 (MQ=255)

 tggcttggctATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTg                                                                                                                                                     <  1:2068154/150‑1 (MQ=255)

   gcttggctCTGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTggg                                                                                                                                                   <  2:3192707/150‑1 (MQ=255)

     ttggctATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTggggg                                                                                                                                                 >  1:2219557/1‑150 (MQ=255)

     ttggctATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTggggg                                                                                                                                                 >  2:3864301/1‑150 (MQ=255)

      tggctATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAACTTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGa                                                                                                                                                <  2:1634333/150‑1 (MQ=255)

          tATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGACTATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCg                                                                                                                                            >  1:1346205/1‑150 (MQ=255)

          tATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCg                                                                                                                                            >  1:1276333/1‑150 (MQ=255)

          tATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCg                                                                                                                                            >  2:3222679/1‑150 (MQ=255)

          tATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCg                                                                                                                                            <  2:3118555/150‑1 (MQ=255)

          tATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCg                                                                                                                                            >  2:3222919/1‑150 (MQ=255)

             gCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGt                                                                                                                                         >  1:910774/1‑150 (MQ=255)

             gCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGt                                                                                                                                         >  2:2998814/1‑150 (MQ=255)

             gCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGt                                                                                                                                         >  2:893034/1‑150 (MQ=255)

              ccAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTg                                                                                                                                        >  1:1038789/1‑150 (MQ=255)

              ccAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTg                                                                                                                                        >  1:3887239/1‑150 (MQ=255)

                tgCTTGCCGAAGCTGGATTATCTGTGGGAAAGTCTGGTTAATGAAATTTATCGCGGGGAAGACAGTTTTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGGTGGATGTGGTGCTTATGCTGGGGGTGGCGGGTggg                                                                                                                                      <  2:30257/149‑1 (MQ=255)

                 gCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTgggg                                                                                                                                     <  2:1139888/150‑1 (MQ=255)

                 gCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTgggg                                                                                                                                     >  2:3257822/1‑150 (MQ=255)

                      ccGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGgcgcgc                                                                                                                                >  2:594655/1‑150 (MQ=255)

                      ccGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGACTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGgcgcgc                                                                                                                                >  2:2484795/1‑150 (MQ=255)

                         aaGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCaat                                                                                                                             >  2:1687441/1‑150 (MQ=255)

                          aGCTGTATTATCAGTCGTAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCaata                                                                                                                            <  2:4121039/150‑1 (MQ=255)

                             tGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAataata                                                                                                                         >  2:3444602/1‑150 (MQ=255)

                             tGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAataata                                                                                                                         >  2:3152847/1‑150 (MQ=255)

                              ggATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAatat                                                                                                                        >  1:1002753/1‑150 (MQ=255)

                               gATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATg                                                                                                                       <  2:1979120/150‑1 (MQ=255)

                                 ttATCTTTCGTAACGTCTGGTTAATTAAATTTTTGGGGGGTATTAAATTTTTGCCCGCCATTGGCTTAACGCGCCTTGTAATATGGACGGCTGGCGGTTGTATTTGGTGCTTTTGCT‑GGGGAGGCGGGTGGGGCGCGCAATAATATgcag                                                                                                                    <  2:3926559/150‑1 (MQ=255)

                                  tATCAGTCGGAAAGTCTGTTGATTTAATTTTATCGCGGGTAAGACAGTTTTGTCCGCCACTGGCTGAAAGCGCCGTGTAATATGGACGGCTGGCGGCTGGTTGTGGTGCATATGCTGGGGGAGGGGGGTGGGGCGCGCAATAATATgcag                                                                                                                    <  1:1167812/150‑1 (MQ=255)

                                   aTCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATgcagc                                                                                                                   >  2:874525/1‑150 (MQ=255)

                                        tCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGtt                                                                                                              >  2:3999405/1‑150 (MQ=255)

                                           gAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGGTGGGGGTGGCGGGTGGGGCGCGCTATAATATGCAGCTCGTTGcc                                                                                                           >  2:2497511/1‑150 (MQ=255)

                                            aaaGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCg                                                                                                          <  2:2826133/150‑1 (MQ=255)

                                              aGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGCGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCggg                                                                                                        <  2:1090399/150‑1 (MQ=255)

                                                tCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGAt                                                                                                      >  2:1158730/1‑150 (MQ=255)

                                                tCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGAt                                                                                                      >  1:687298/1‑150 (MQ=255)

                                                tCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGAt                                                                                                      <  1:3032994/150‑1 (MQ=255)

                                                  tGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCa                                                                                                    >  2:268464/1‑150 (MQ=255)

                                                  tGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCa                                                                                                    >  1:724541/1‑150 (MQ=255)

                                                    gTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCTTTTGCTGGGGGAGGCGGGTGGGGCGCGCAATATTATGCAGCACGTTGCCGGGATCAcc                                                                                                  >  2:767127/1‑150 (MQ=255)

                                                       aattaaATTTATGGCGGGGAAGACTGTTTGGTCCGCCACGGGCTGAATGCGCCGTGGAATATGGCGGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGGGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGaa                                                                                               <  1:517315/150‑1 (MQ=255)

                                                        atgaaATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAg                                                                                              >  1:1317730/1‑150 (MQ=255)

                                                         tgaaATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAgc                                                                                             >  1:2966578/1‑150 (MQ=255)

                                                          gaaATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAACGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAgcg                                                                                            <  2:1112130/150‑1 (MQ=255)

                                                          gaaATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCTTGGAATATGTACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAgcg                                                                                            <  2:1086038/150‑1 (MQ=255)

                                                          gaaATTTATCGCGGGGAAGACAGTATTGTCCGCCACGGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGGGGTGCATATGCGGGGGGCGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAgcg                                                                                            <  2:1314364/150‑1 (MQ=255)

                                                             aTTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAgcggcg                                                                                         >  2:3762981/1‑150 (MQ=255)

                                                              tttATCGCGGGGACGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCga                                                                                        <  1:3095855/150‑1 (MQ=255)

                                                                   cgcgGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCgaaagaa                                                                                   <  1:3546171/150‑1 (MQ=255)

                                                                    gcgGGGAAGACTGTATTGTCCGCCACTGGCTGAATGCGCCGTGTAATATGGACGGCTGGGGGGTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCgaaagaaa                                                                                  <  2:723512/150‑1 (MQ=255)

                                                                    gcgGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCgaaagaaa                                                                                  <  2:810659/150‑1 (MQ=255)

                                                                    gcgGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCgaaagaaa                                                                                  <  2:723374/150‑1 (MQ=255)

                                                                     cgGGTAAGACAGTATTGTCCGCCACTGTCTGAAAGCGCGGTGTAATTTGGACGGCTGGGGGCTGGATGGGGTCCATTGGCTGGGGGCGGGGGGTGGGGCGCGAAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAAc                                                                                 <  1:915397/150‑1 (MQ=255)

                                                                              cAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGa                                                                        >  1:3746657/1‑150 (MQ=255)

                                                                               aGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGaa                                                                       >  2:4133296/1‑150 (MQ=255)

                                                                               aGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCGGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGaa                                                                       >  2:1816572/1‑150 (MQ=255)

                                                                                gTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAg                                                                      <  2:1359011/150‑1 (MQ=255)

                                                                                 tATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGc                                                                     >  1:3346982/1‑150 (MQ=255)

                                                                                                gCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGa                                                      >  2:68502/1‑150 (MQ=255)

                                                                                                 cTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGaa                                                     <  2:3887239/150‑1 (MQ=255)

                                                                                                  tGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAAc                                                    >  1:536208/1‑150 (MQ=255)

                                                                                                   gAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACa                                                   >  1:1093335/1‑150 (MQ=255)

                                                                                                   gAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACa                                                   >  1:265947/1‑150 (MQ=255)

                                                                                                   gAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACa                                                   >  1:1686979/1‑150 (MQ=255)

                                                                                                      aGCGCCGTGGAATCTGGACGGCTGGCGGCTGGATGTGGTGCATATGCGGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACAttt                                                <  2:2451717/150‑1 (MQ=255)

                                                                                                       gcgcCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACAtttt                                               <  2:1898165/150‑1 (MQ=255)

                                                                                                       gcgcCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACAtttt                                               <  2:524073/150‑1 (MQ=255)

                                                                                                         gcCGTGGAATTTGGCCGGGTGGGGGCTGGATGTGGTGCATATGCGGGGGGCGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTgg                                             <  1:3257822/150‑1 (MQ=255)

                                                                                                          ccGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGCGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGc                                            <  1:1588518/150‑1 (MQ=255)

                                                                                                          ccGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGc                                            <  2:2582291/150‑1 (MQ=255)

                                                                                                          ccGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCGGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGc                                            <  2:1073477/150‑1 (MQ=255)

                                                                                                               gAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGc                                       <  2:3707461/150‑1 (MQ=255)

                                                                                                                aaTATGGCCGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCa                                      <  1:3480901/150‑1 (MQ=255)

                                                                                                                aaTATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCa                                      <  1:124383/150‑1 (MQ=255)

                                                                                                                aaTATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCa                                      <  1:1643906/150‑1 (MQ=255)

                                                                                                                aaTATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCa                                      <  1:2203230/150‑1 (MQ=255)

                                                                                                                aaTATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCa                                      <  1:2617502/150‑1 (MQ=255)

                                                                                                                aaTATGGACGGCTGGCGGCTGGATGTGGTGCATATGCGGGGGGCGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCa                                      <  1:4028182/150‑1 (MQ=255)

                                                                                                                 atatGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCAc                                     >  2:2329632/1‑150 (MQ=255)

                                                                                                                 atatGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCAc                                     >  2:1764258/1‑150 (MQ=255)

                                                                                                                        cggctggcggctggATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATg                              <  2:221456/150‑1 (MQ=255)

                                                                                                                           ctggcggctggATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTTGCGATGCACGGCAATGGtt                           >  2:2038129/1‑150 (MQ=255)

                                                                                                                              gcggctggATGTGGTGCATATGCGGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACa                        <  1:287324/150‑1 (MQ=255)

                                                                                                                                 gctggATGTGGTGCATATGCTGGGGGCGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGc                     <  2:2051100/150‑1 (MQ=255)

                                                                                                                                   tggATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCg                   >  1:1825278/1‑150 (MQ=255)

                                                                                                                                    ggATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCGa                  <  1:1842380/150‑1 (MQ=255)

                                                                                                                                        gtggtgCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCGAtgtg              <  2:1557156/150‑1 (MQ=255)

                                                                                                                                         tggtgCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCGATGTgg             >  1:4201649/1‑150 (MQ=255)

                                                                                                                                         tggtgCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCGATGTgg             <  2:1419957/150‑1 (MQ=255)

                                                                                                                                         tggtgCATATGCGGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCGATGTgg             <  2:1217648/150‑1 (MQ=255)

                                                                                                                                          ggtgCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCGATGTGGa            <  1:2494434/150‑1 (MQ=255)

                                                                                                                                          ggtgCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCGATGTGGa            >  2:2967045/1‑150 (MQ=255)

                                                                                                                                          ggtgCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCGATGTGGa            <  1:3554076/150‑1 (MQ=255)

                                                                                                                                          ggtgCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCGATGTGGa            >  2:961683/1‑150 (MQ=255)

                                                                                                                                           gtgCATTGGCTGGGGGAGGCGGGTGGGGCGCGAAATAATATGCAGCACGTTGCCGGGATAACCGAAGCGGCGAAAGAAACCCAGCCGTAAGCTTATATTGTCGGAGATCATTTTGGCGATGCACGTCAATGGTTACAGGCCTATGTGTaa           <  2:3721288/150‑1 (MQ=255)

                                                                                                                                                tatGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCGATGTGGAAGATgc      <  1:1514847/150‑1 (MQ=255)

                                                                                                                                                   gCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCGATGTGGAAGATgccgc   <  2:3540492/150‑1 (MQ=255)

                                                                                                                                                    cTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCGATGTGGAAGATgccgcc  >  1:4132681/1‑150 (MQ=255)

CTGGCTTGGCTATGCCAGCTTGCCGAAGCTGGATTATCAGTCGGAAAGTCTGGTGAATGAAATTTATCGCGGGGAAGACAGTATTGTCCGCCACTGGCTGAAAGCGCCGTGGAATATGGACGGCTGGCGGCTGGATGTGGTGCATATGCTGGGGGAGGCGGGTGGGGCGCGCAATAATATGCAGCACGTTGCCGGGATCACCGAAGCGGCGAAAGAAACCCAGCCGGAAGCGTATATTGTCGGCGAACATTTTGGCGATGCACGGCAATGGTTACAGGCCGATGTGGAAGATGCCGCC  >  NC_000913/423390‑423687

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`