Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 756,886 T→G 86.7% L327R (CTG→CGG)  sdhA → succinate dehydrogenase, flavoprotein subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913756,8860TG86.7% 873.3 / 92.9 267L327R (CTG→CGG) sdhAsuccinate dehydrogenase, flavoprotein subunit
Reads supporting (aligned to +/- strand):  ref base T (17/19);  new base G (124/107);  total (141/126)
Fisher's exact test for biased strand distribution p-value = 4.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 267 total aligned reads displayed.

GAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGA  >  NC_000913/756740‑757033
                                                                                                                                                  |                                                                                                                                                   
gAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGcc                                                                                                                                                  >  2:1247366/1‑150 (MQ=255)
  aCGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCgg                                                                                                                                                <  2:1151741/150‑1 (MQ=255)
      cAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGACACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGtct                                                                                                                                            >  1:3082753/1‑148 (MQ=255)
      cAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTAt                                                                                                                                            >  2:1982195/1‑150 (MQ=255)
      cAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTAt                                                                                                                                            >  2:1130934/1‑150 (MQ=255)
      cAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTAt                                                                                                                                            >  2:3656071/1‑150 (MQ=255)
      cAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTAt                                                                                                                                            >  1:2307123/1‑150 (MQ=255)
       aaaGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATc                                                                                                                                           >  1:1387843/1‑150 (MQ=255)
       aaaGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATc                                                                                                                                           >  1:890080/1‑150 (MQ=255)
       aaaGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATc                                                                                                                                           >  2:3567034/1‑150 (MQ=255)
         aGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCt                                                                                                                                         <  1:2497656/150‑1 (MQ=255)
          gACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTg                                                                                                                                        <  1:324940/150‑1 (MQ=255)
          gACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTg                                                                                                                                        <  1:2448918/150‑1 (MQ=255)
           aCCTGGCGGGCCGTGACGTGGTTGCGCGTTCCAACATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTgg                                                                                                                                       <  2:1959343/150‑1 (MQ=255)
            ccTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGa                                                                                                                                      <  1:1167753/150‑1 (MQ=255)
             cTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAg                                                                                                                                     >  1:1825502/1‑150 (MQ=255)
              tGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGc                                                                                                                                    >  2:448846/1‑150 (MQ=255)
               ggCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCt                                                                                                                                   >  1:787299/1‑150 (MQ=255)
                gCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCtt                                                                                                                                  >  1:3980277/1‑150 (MQ=255)
                  gggCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTc                                                                                                                                <  1:1274154/150‑1 (MQ=255)
                   ggCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTcc                                                                                                                               <  1:3363919/150‑1 (MQ=255)
                        tGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTAc                                                                                                                          <  2:862569/150‑1 (MQ=255)
                          aCGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCt                                                                                                                        >  2:3964072/1‑150 (MQ=255)
                           cGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCtt                                                                                                                       <  1:3102519/150‑1 (MQ=255)
                               gTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCt                                                                                                                   <  1:3225679/150‑1 (MQ=255)
                               gTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCt                                                                                                                   >  2:1502303/1‑150 (MQ=255)
                                  gcgcGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCAc                                                                                                                <  2:74630/150‑1 (MQ=255)
                                   cgcgTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTc                                                                                                                                <  1:2975623/133‑1 (MQ=255)
                                   cgcgTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTc                                                                                                                                >  2:2975623/1‑133 (MQ=255)
                                    gcgTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGt                                                                                                              >  1:3609317/1‑150 (MQ=255)
                                       ttCCATCATGATCGTAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGa                                                                                                           <  1:61389/150‑1 (MQ=255)
                                       ttCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGa                                                                                                           <  1:3487590/150‑1 (MQ=255)
                                           atcatGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCg                                                                                                       >  1:498523/1‑150 (MQ=255)
                                            tcatGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCgg                                                                                                      >  2:1820084/1‑150 (MQ=255)
                                            tcatGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCgg                                                                                                      <  1:541656/150‑1 (MQ=255)
                                              atGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTg                                                                                                    >  2:549183/1‑150 (MQ=255)
                                              atGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTg                                                                                                    >  2:495283/1‑150 (MQ=255)
                                                gATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGaa                                                                                                  <  1:2907438/150‑1 (MQ=255)
                                                gATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGaa                                                                                                  <  1:3787526/150‑1 (MQ=255)
                                                  tCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAag                                                                                                >  1:2189276/1‑150 (MQ=255)
                                                   cGAAATCCGTGCAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAaga                                                                                               <  1:2592711/150‑1 (MQ=255)
                                                                 gTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTa                                                                                 >  2:2338242/1‑150 (MQ=255)
                                                                  tCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTAt                                                                                >  2:431883/1‑150 (MQ=255)
                                                                  gcgcgGCTGGGCTGGTCCGTGGGGGCCACACGCGCCCCTGAACCTCGATCACCTGGGTAAAGCAGTTCTCGACTCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCCCGTCGATCCGGTGAAAGAGCCGATTCCGGTTAt                                                                                <  2:3098782/149‑1 (MQ=255)
                                                                       gCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCaa                                                                           <  2:2310254/150‑1 (MQ=255)
                                                                       gCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCaa                                                                           <  2:1910940/150‑1 (MQ=255)
                                                                             aTGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTc                                                                     >  2:725491/1‑150 (MQ=255)
                                                                                 tCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTa                                                                 <  1:3194516/150‑1 (MQ=255)
                                                                                  ccGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTAc                                                                <  2:3080924/150‑1 (MQ=255)
                                                                                   cGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGGAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACa                                                               >  2:2391606/1‑150 (MQ=255)
                                                                                    gTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACat                                                              <  1:3691562/150‑1 (MQ=255)
                                                                                    gTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACat                                                              <  1:1429667/150‑1 (MQ=255)
                                                                                        gggCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACatgatg                                                          >  1:3958915/1‑150 (MQ=255)
                                                                                         ggCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATgg                                                         >  2:1221787/1‑150 (MQ=255)
                                                                                         ggCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATgg                                                         >  1:834810/1‑150 (MQ=255)
                                                                                         ggCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATgg                                                         >  2:4117607/1‑150 (MQ=255)
                                                                                            cacaCGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCg                                                      >  2:1182838/1‑150 (MQ=255)
                                                                                             acacGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCgg                                                     >  2:3861524/1‑150 (MQ=255)
                                                                                                 gcgAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTAtt                                                 >  2:1366671/1‑150 (MQ=255)
                                                                                                 gcgAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTAtt                                                 >  1:1989502/1‑150 (MQ=255)
                                                                                                  cgAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTc                                                <  1:3831421/150‑1 (MQ=255)
                                                                                                   gaaactgaaactCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTcc                                               <  2:1078907/150‑1 (MQ=255)
                                                                                                   gaaactgaaactCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTcc                                               >  1:4046627/1‑150 (MQ=255)
                                                                                                   gaaactgaaactCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTcc                                               <  1:3304499/150‑1 (MQ=255)
                                                                                                   gaaactgaaactCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTcc                                               <  2:2235478/150‑1 (MQ=255)
                                                                                                         gaaactCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCaa                                         >  2:1673758/1‑150 (MQ=255)
                                                                                                          cacctCGATCACCTGGGTAAAGAAGTTCTCGCATCCCGTCGGCCGGGTATCCTGGAGCGTTCCCGGACCTTCGCTCACGTCGATCCGGGGAAAGAGCCGATTCCGGTGATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCaaa                                        <  2:948410/147‑1 (MQ=255)
                                                                                                          aaactCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCaaa                                        >  1:1411308/1‑150 (MQ=255)
                                                                                                          aaactCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCaaa                                        <  2:3643073/150‑1 (MQ=255)
                                                                                                              tCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTa                                    <  2:608865/150‑1 (MQ=255)
                                                                                                              tCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTa                                    >  1:2394528/1‑150 (MQ=255)
                                                                                                               cGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTAc                                   <  1:2500460/150‑1 (MQ=255)
                                                                                                               cGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTAc                                   <  2:3348419/150‑1 (MQ=255)
                                                                                                                gATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTAcc                                  >  1:4148668/1‑150 (MQ=255)
                                                                                                                gATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTAcc                                  >  2:3404234/1‑150 (MQ=255)
                                                                                                                 aTCACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCg                                 <  2:3943284/150‑1 (MQ=255)
                                                                                                                   cACCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGt                               <  2:2938698/150‑1 (MQ=255)
                                                                                                                    aCCTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAAACTGTCACTACATGATGGGCGGTATTCAGACCAAAGTTAACGGTc                              >  1:270002/1‑150 (MQ=255)
                                                                                                                     ccTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCa                             <  1:929692/150‑1 (MQ=255)
                                                                                                                     ccTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCa                             <  2:3899586/150‑1 (MQ=255)
                                                                                                                     ccTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCa                             >  1:1142748/1‑150 (MQ=255)
                                                                                                                     ccTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTGTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCa                             <  2:269638/150‑1 (MQ=255)
                                                                                                                      cTGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAg                            >  2:4020540/1‑150 (MQ=255)
                                                                                                                       tGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAgg                           <  2:2636004/150‑1 (MQ=255)
                                                                                                                       tGGGTAAAGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAgg                           >  2:2480481/1‑150 (MQ=255)
                                                                                                                          gTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCAGTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCac                        >  1:281597/1‑150 (MQ=255)
                                                                                                                           tAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCact                       <  2:55473/150‑1 (MQ=255)
                                                                                                                            aaaGAAGTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCactg                      >  1:4097173/1‑150 (MQ=255)
                                                                                                                               gaagTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCactgact                   <  2:1023361/150‑1 (MQ=255)
                                                                                                                               gaagTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCactgact                   <  2:206637/150‑1 (MQ=255)
                                                                                                                                aagTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCactgactg                  <  2:2257767/150‑1 (MQ=255)
                                                                                                                                 agTTCTCGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTGTCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACtgt                 <  1:1568844/150‑1 (MQ=255)
                                                                                                                                       cGAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTGCATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATga           <  1:1356755/150‑1 (MQ=255)
                                                                                                                                        gAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATgag          >  2:1875253/1‑150 (MQ=255)
                                                                                                                                        gAATCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATgag          >  1:1010520/1‑150 (MQ=255)
                                                                                                                                         aaTCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATgaga         <  2:1711287/150‑1 (MQ=255)
                                                                                                                                         aaTCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATgaga         >  2:2620727/1‑150 (MQ=255)
                                                                                                                                           tCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGgaa       >  1:1661115/1‑150 (MQ=255)
                                                                                                                                           tCCCGTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGaaa       <  2:1994083/150‑1 (MQ=255)
                                                                                                                                               gTCGGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCg   >  1:1524997/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
GAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGA  >  NC_000913/756740‑757033

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: