Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 781,668 C→T 5.1% intergenic (+16/‑417) lysQ → / → nadA tRNA‑Lys/quinolinate synthase, subunit A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913781,6680CT 5.1% 737.1 / 19.2 234intergenic (+16/‑417)lysQ/nadAtRNA‑Lys/quinolinate synthase, subunit A
Reads supporting (aligned to +/- strand):  ref base C (120/101);  new base T (10/2);  total (130/104)
Fisher's exact test for biased strand distribution p-value = 7.11e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 244 total aligned reads displayed.

AGTCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAA  >  NC_000913/781522‑781817
                                                                                                                                                  |                                                                                                                                                     
acctcccttacaaggagggggtcggcggttcgatcccgtcatcacccaccacttcGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTaa                                                                                                                                                    <  2:1566107‑M1/95‑1 (MQ=255)
 cctcccttacatggagggggtcggcggttcgatcccgtcatcacccaccacttgGGGTCTTTAGCTCAGTTGGTAGAGCTGTTGACTTTTAATCATTTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTaaa                                                                                                                                                   >  1:1402431‑M1/55‑150 (MQ=255)
  tCATCCTGCACGACCCACCATCCTTAATTATTAAGGCAGCATAATCCCGCTAGGGGTCGTTAGCTCAGTTGGTAGAGCTGTTTACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAAAAGCGCCCTAAAG                                                                                                                                                  <  1:869226‑M1/150‑1 (MQ=255)
  tCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAG                                                                                                                                                  <  2:3468492‑M1/150‑1 (MQ=255)
  ctcccttacaaggagggggtcggcggttcgatcccgtcatcacccaccacttcGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCTGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAg                                                                                                                                                  >  1:2255645‑M1/54‑150 (MQ=255)
     tCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAATCGCCCTAAAGGCG                                                                                                                                               >  1:133093‑M1/1‑150 (MQ=255)
     ccttacaaggaggggggcggcggttcgatcccgtcatcacccaccacttcGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGgcg                                                                                                                                               <  2:3387684‑M1/100‑1 (MQ=255)
      cttacaaggagggggtcggcggttcgatcccgtcatcacccaccacttcGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGgcgc                                                                                                                                              >  2:116049‑M1/50‑150 (MQ=255)
      cttacaaggagggggtcggcggttcgatcccgtcatcacccaccacttcGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGgcgc                                                                                                                                              >  2:2459683‑M1/50‑150 (MQ=255)
           aCGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTT                                                                                                                                         <  2:2618338‑M1/150‑1 (MQ=255)
           aCGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTT                                                                                                                                         >  1:2265761‑M1/1‑150 (MQ=255)
             gACCCACCATCCTTAATGATTACGGCAGCATAATCCCGCTTGGGGTGGTTTGCTCTGTTGTTAGTGCTGTTGACTTTTAATCTTTTGGTCGCTGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCTTTAGGGCGCtttttta                                                                                                                                       <  1:711946/150‑2 (MQ=17)
             gACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTG                                                                                                                                       >  1:3976726‑M1/1‑150 (MQ=255)
             gACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTG                                                                                                                                       <  2:1892196‑M1/150‑1 (MQ=255)
              aCCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGC                                                                                                                                      >  1:2210344‑M1/1‑150 (MQ=255)
                 caccaTCCTTAATGATTAAGGAAGCTTAAGCCCCGAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTAT                                                                                                                                   <  1:3073243‑M1/150‑1 (MQ=255)
                 caccaTCCTGAATGATTAAGGCAGCATAATCCTGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTAT                                                                                                                                   <  1:928927‑M1/150‑1 (MQ=255)
                   ccaTCCTGAATGATTAAGGCATCATAAGGCCGCTTGGGGTCTTTAGCTCAGTGGGTAGTGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCt                                                                                                                                 <  1:2090834/150‑1 (MQ=17)
                      tCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTGCTATCTGCGa                                                                                                                             <  1:122045/150‑1 (MQ=255)
                       ccTGAATGATTAAGGCAGCCTAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTACAGGCGCTTTTTTGCTATCTGCGa                                                                                                                             <  2:2852943/150‑1 (MQ=255)
                       ccTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGa                                                                                                                             <  1:1332306/150‑1 (MQ=255)
                         tGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATa                                                                                                                           <  1:3410550/150‑1 (MQ=255)
                               aTTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCaaa                                                                                                                     >  2:2812544/1‑150 (MQ=255)
                                    ggCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTc                                                                                                                <  2:144751/150‑1 (MQ=255)
                                       agcaTAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGaa                                                                                                             <  2:1623154/150‑1 (MQ=255)
                                        gcaTAATCCCGCCAGGGGTCGTGAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAAc                                                                                                            <  1:813411/150‑1 (MQ=255)
                                             aTCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATtcaggcatcctcaatttcactttgtaaa                                                                                                       <  2:2265761‑M1/150‑29 (MQ=255)
                                                ccGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCGATCTGCGATACTCAAAGATTCGAACCTgcagcc                                                                                                    >  2:1314419/1‑149 (MQ=38)
                                                  gCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAgg                                                                                                  >  2:752396/1‑150 (MQ=35)
                                                  gCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAgg                                                                                                  >  2:3269927/1‑150 (MQ=35)
                                                     aGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGttt                                                                                               <  1:2812544/150‑1 (MQ=33)
                                                           cGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTttgagttg                                                                                         <  1:3823218/150‑1 (MQ=32)
                                                            gTTAGCTCAGTTGGTAGAGCAGTTGACTTTTACTCACTTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTttgagttga                                                                                        <  2:3914020/150‑1 (MQ=17)
                                                            gTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTttgagttga                                                                                        >  1:190506/1‑150 (MQ=32)
                                                                 cTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATtcaggcatcctcaatttcactttgtcaacctgatgacatcgtcagagc                                                                                   >  2:3342215‑M1/1‑102 (MQ=255)
                                                                 cTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCa                                                                                   <  1:2939113/150‑1 (MQ=32)
                                                                  tCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAg                                                                                  >  1:3495449/1‑150 (MQ=32)
                                                                  tCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAg                                                                                  <  1:1130399/150‑1 (MQ=32)
                                                                     gTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGAAGCTGGTTTGAGATGAGCTCAgaga                                                                               >  2:2339446/1‑150 (MQ=11)
                                                                     gTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATAATCGATACACAAATATAAGTAACTGAACCTGGTTTGAGGTGAtcgccgaga                                                                               >  2:1638709/1‑145 (MQ=11)
                                                                       tGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGaaa                                                                             <  1:100831/150‑1 (MQ=32)
                                                                        ggTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAaac                                                                            >  2:1935175/1‑150 (MQ=32)
                                                                        ggTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAaac                                                                            >  2:1936220/1‑150 (MQ=32)
                                                                         gTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAaaca                                                                           >  2:2397446/1‑150 (MQ=32)
                                                                              tcAGTTGACTTTTAATCTATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGa                                                                      <  2:649537/149‑1 (MQ=17)
                                                                              gCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAAACTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGa                                                                      >  1:4011027/1‑150 (MQ=17)
                                                                                 gTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGcc                                                                   >  2:1419961/1‑150 (MQ=32)
                                                                                    gACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCt                                                                >  1:797510/1‑150 (MQ=32)
                                                                                    gACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCt                                                                >  1:250784/1‑150 (MQ=32)
                                                                                     aCTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGCTACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTc                                                               >  1:3116736/1‑150 (MQ=17)
                                                                                       ttttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCCAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAACCAACGGAGCCGCTcgc                                                             >  2:457177/1‑150 (MQ=17)
                                                                                       ttttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAAACTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTcgc                                                             >  1:335600/1‑150 (MQ=17)
                                                                                       ttttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATATGCGATACACAAAGATACGAACCTGAACCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTcgc                                                             >  1:4148115/1‑150 (MQ=11)
                                                                                             tCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGAc                                                       >  1:1068126/1‑150 (MQ=32)
                                                                                             tCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATATGCGAAACACAAATATTCGAACCTGAAGCAGGTTTGAGTTGAGCGCAGCGAGACAACGGAGCCGCTAGCGTCGAc                                                       >  2:4116163/1‑150 (MQ=11)
                                                                                               aaTTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACGACGGAGCCGCTCGCGGCGACgg                                                     >  2:2539358/1‑150 (MQ=17)
                                                                                               aaTTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGATATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACgg                                                     <  2:3699353/150‑1 (MQ=17)
                                                                                                     tCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCAGCGATACTAAAATATACGATACTGCAGCAGGTTTGATGTGATCGCAGCGAAGCTACGGCGCCGCTCGCGGCGACGGCCAGaa                                               >  1:16934/1‑150 (MQ=11)
                                                                                                          ggTTCGAATCCTGCACGACCCACCAATGTAAAAATGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGgcg                                          <  2:2359324/150‑1 (MQ=17)
                                                                                                            ttCGACTCCGGCACCCCCCACCAATTAAAAAAAGCGCCCTAACGGCGCTTTTTTGCTATCTGCGATACACAAAGATTCGACCCTGCCGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGgcgag                                        <  2:1917591/150‑1 (MQ=11)
                                                                                                                aaTCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTGTTTTGCTATCTGCGATACTCAAAGATGCGAACCTGCAGCAGGTGGGAGGTGAGCGCAGCGAACCAACGGAGCCGCTCGCGGCGACGGCCCGCCGGgcgcgcgcc                                    >  2:326169/1‑148 (MQ=11)
                                                                                                                  tCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATtcaggcatcctcaatttcactttgtaaacctgatgacatcgtcagagcttactgtgcaagcaactctatgtcggtggaattaggcgtaaaatgacgc                                  >  2:2197783‑M1/1‑53 (MQ=255)
                                                                                                                  tCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGagcgaagc                                  >  1:1433461/1‑150 (MQ=32)
                                                                                                                  tCCTGCACGACCCACCAATGTAAAAAAGCGCCATAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCTGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGagcgaagc                                  <  2:1237932/150‑1 (MQ=17)
                                                                                                                    cTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATtcaggcatcctcaatttcactttgtaaacctgatgacatcgtcagagcttactgtgcaagcaactctatgtcggtggaattaggcgtaaaatgacgcat                                >  1:1151334‑M1/1‑51 (MQ=255)
                                                                                                                      gCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATtcaggcatcctcaatttcactttgtaaacctgatgacatcgtcagagcttactgtgcaagcacctctatgtcggtggaattaggcgtaaaatgacgcatcc                              >  1:547393‑M1/1‑49 (MQ=255)
                                                                                                                        aCGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATtcaggcatcctcaatttcactttttaaacctgatgacatcgtcagagcttactgtgcaagcaactctatgtcggtggaattaggcgtaaaatgacgcatcctg                            <  2:133093‑M1/150‑104 (MQ=255)
                                                                                                                          gACCCACCCATGTAAAAAAGCGCCCTACAGGCGCTTTTTTGCTATtcaggcatcctcaatttcactttgtaaacctgatgacatcgtcagagcttactgtgcaagcacctctctggcggtggacttaggcgtaaaatgacgcatcctgca                          >  2:8948‑M1/1‑45 (MQ=255)
                                                                                                                          gACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATtcaggcatcctcaatttcactttgtaaacctgatgacatcgtcagagcttactgtgcaagcaactctatgtcggtggaattaggcgtaaaatgacgcatcctgca                          >  2:2067872‑M1/1‑45 (MQ=255)
                                                                                                                          gACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGCGTCATc                          >  1:2896384/1‑150 (MQ=17)
                                                                                                                             ccaccaATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAATGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGACGGGCGAGCGAAGCGAGTCATCCTg                       >  2:1213749/1‑150 (MQ=17)
                                                                                                                             ccaccaATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGATCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGGCATCCTg                       >  1:1263686/1‑150 (MQ=17)
                                                                                                                               accaATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATtcaggcatcctcaatttcactttgtaaacctgatgacatcgtcagagcttactgtgcaagcaactctatgtcggtggaattaggcgtaaaatgacgcatcctgcacatta                     >  2:876263‑M1/1‑40 (MQ=255)
                                                                                                                               accaATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATtcaggcatcctcaatttcactttgtaaacctgatgacatcgtcagagcttactgtgcaagcaactctatgtcggtggaattaggcgtaaaatgacgcatcctgcacatta                     <  2:465347‑M1/150‑111 (MQ=255)
                                                                                                                                 caATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATtcaggcatcctcaatttcactttgtaaacctgatgacatcgtcagagcttactgtgcaagcaactctatgtcggtggaattaggcgtaaaatgacgcatcctgcacattagg                   <  1:1180044‑M1/150‑113 (MQ=255)
                                                                                                                                 caATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATtcaggcatcctcaatttcactttgtaaacctgatgacatcgtcagagcttactgtgcaagcaactctatgtcggtggaattaggcgtaaaatgacgcatcctgcacattagg                   <  1:1912375‑M1/150‑113 (MQ=255)
                                                                                                                                 caATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATtcaggcatcctcaatttcactttgtaaacctgatgacatcgtcagagcttactgtgcaagcaactctatgtcggtggaattaggcgtaaaatgacgcatcctgcacattagg                   >  2:1803038‑M1/1‑38 (MQ=255)
                                                                                                                                    tGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATtcaggcatcctcaatttcactttgtaaacctgatgacatcgtcagagcttactgtgcaagcaactctatgtcggtggaattaggcgtaaaatgacgcatcctgcacattaggcgt                >  2:2699197‑M1/1‑35 (MQ=255)
                                                                                                                                     gTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATtcaggcatcctcaatttcactttgtaaacctgatgacatcgtcagagcttactgtgcaagcaactctatgtcggtggaattaggcgtaaaatgacgcatcctgcacattaggcgta               >  2:2677642‑M1/1‑34 (MQ=255)
                                                                                                                                      tAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATtcaggcatcctcaatttcactttgtaaacctgatgacatagtcagagcttactgtgcaagcaactctatgtcggtggaattaggcgtaaaatgatgcatcctgcacattaggcgtaa              >  1:3931232‑M1/1‑33 (MQ=255)
                                                                                                                                       aaaaaaGCGCCTTTAGGGCGCTTTTTTGCTATtcaggcatcctcaatttcactttgtaaacctgatgacatcgtcagagcttactgtgcaagcaactctatgtcggtggaattaggcgtaaaatgacgcatcctgcacattaggcgtaat             >  2:3527394‑M1/1‑32 (MQ=255)
                                                                                                                                       aaaaaaGCGCCCTAAAGGCGCTTTTTTGCTATtcaggcatcctcaatttcactttgtaaacctgatgacatcgtcagagcttactgtgcaagcaactctatgtcggtggaattaggcgtaaaatgacgcatcctgcacattaggcgtaat             <  2:2127333‑M1/150‑119 (MQ=255)
                                                                                                                                       aaaaaaGCGCCCTAAAGGCGCTTTTTTGCTATtcaggcatcctcaatttcactttgtaaacctgatgacatcgtcagagcttactgtgcaagcaactctatgtcggtggaattaggcgtaaaatgacgcatcctgcacattaggcgtaat             <  2:1390104‑M1/150‑119 (MQ=255)
                                                                                                                                       aaaaaaGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACccac             >  2:3348217/1‑150 (MQ=32)
                                                                                                                                        aaaaaGCGCCTTTAGGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTAGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACccacc            >  1:959622/1‑150 (MQ=255)
                                                                                                                                         aaaaGCGCCTTTAGGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACccacca           >  1:2988487/1‑150 (MQ=12)
                                                                                                                                         aaaaGCGCCCTAAAGGCGCTTTTTTTCTATtaaggcatcctcaatttcactttgtaaaactgattacatcgtcagagcttacagtgcaagcaactctatgtcggtggaattaggcgtaaaatgacgcatcctgcacattaggcgtaattc           >  1:2018167‑M1/1‑30 (MQ=255)
                                                                                                                                         aaaaGCGCCCTAAAGGCGCTTTTTTGCTATtcaggcatcctcaatttcactttgtaaacctgatgacatcgtcagagcttactgtgcaagcaactctatgtcggtggaattaggcgtaaaatgacgcatcctgcacattaggcgtaattc           >  1:2568710‑M1/1‑30 (MQ=255)
                                                                                                                                         aaaaGCGCCCTAAAGGCGCTTTTTTGCTATtcaggcatcctcaatttcactttgtaaacctgatgacatcgtcagagcttactgtgcaagcaactctatgtcggtggaattaggcgtaaaatgacgcatcctgcacattaggcgtaattc           >  2:4003504‑M1/1‑30 (MQ=255)
                                                                                                                                          aaaGCGCCTTTAGGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCaa          >  2:3064301/1‑150 (MQ=12)
                                                                                                                                           aaGCGCCCTCAAGGCGCTTTTTTGCTATtcaggcatcctcaatttcactttgtaaacctgatgacatcgtcagagcttactgtgcaagcaacactatgtcggtggaattaggcgtaaaatgacgcatcctgcacattaggcgtaattcga         <  2:3116736‑M1/150‑123 (MQ=255)
                                                                                                                                           aaGCGCCCTAAAGGCGCTTTTTTGCTATtcaggcatcctcaatttcactttgtaaacctgatgacatcgtcagagcttactgtgcaagcaactctatgtcggtggaattaggcgtaaaatgacgcatcctgcacattaggcgtaattcga         <  2:4011027‑M1/150‑123 (MQ=255)
                                                                                                                                           aaGCGCCCTAAAGGCGCTTTTTTGCTATtcaggcatcctcaatttcactttgtaaacctgatgacatcgtcagagcttactgtgcaagcaactctatgtcggtggaattaggcgtaaaatgacgcatcctgcacattaggcgtaattcga         <  2:571005‑M1/150‑123 (MQ=255)
                                                                                                                                           aaGCGCCCTAAAGGCGCTTTTTTGCTATtcaggcatcctcaatttcactttgtaaacctgatgacatcgtcagagcttactgtgcaagcaactctatgtcggtggaattaggcgtaaaatgacgcatcctgcacattaggcgtaattcga         <  2:805274‑M1/150‑123 (MQ=255)
                                                                                                                                           aaGCGCCCTAAAGGCGCTTTTTTGCTATtcaggcatcctcaatttcactttgtaaacctgatgacatcgtcagagcttactgtgcaagcaactctatgtcggtggaattaggcgtaaaatgacgcatcctgcacattaggcgtaattcga         <  2:2022156‑M1/150‑123 (MQ=255)
                                                                                                                                            aGCGCCCTAAAGGCGCTTTTTTGCTATtcaggcatcctcaatttcactttgtaaacctgatgacatcgtcagagcttactgtgcaagcaactctatgtcggtggaattaggcgtaaaatgacgcatcctgcacattaggcgtaattcgag        <  2:3197824‑M1/150‑124 (MQ=255)
                                                                                                                                                 ccTAAAGGCGCTTTTTTGCTATtcaggcatcctcaatttcactttgtaaacctgatgacatcgtcagagcttactgtgcaagcaactctatgtcggtggaattaggcgtaaaatgacgcatcctgcacattaggcgtaattcgagtgact   >  2:140512‑M1/1‑22 (MQ=255)
                                                                                                                                                  cTAAAGGCGCTTTTTTGCTATtcaggcatcctcaatttcactttgtaaacctgatgacatcgtcagagcttactgtgcaagcaactctatgtcggtggaattatgcgtaaaatgacgcatcctgcacattaggcgtaattcgagtgactt  >  1:3716493‑M1/1‑21 (MQ=255)
                                                                                                                                                  cTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTAGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTaaaaa  >  1:429789/1‑150 (MQ=17)
                                                                                                                                                  cTAAAGGCGCTATTTTGCTATCTGCGATACTCAAAGATACGAACCTGCAGCAGCTTTGAGTTGAGCGCAGCGAAACAAAGCAGCCGCTCGCGGCGACGGCACGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTaaaaa  >  2:1065951/1‑150 (MQ=11)
                                                                                                                                                  |                                                                                                                                                     
AGTCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTGCTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAA  >  NC_000913/781522‑781817

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: