Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 993,255 A→C 5.8% intergenic (+21/+22) pepN → / ← ssuB aminopeptidase N/aliphatic sulfonate ABC transporter ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913993,2550AC 5.8% 853.9 / 27.7 243intergenic (+21/+22)pepN/ssuBaminopeptidase N/aliphatic sulfonate ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base A (106/122);  new base C (4/11);  total (110/133)
Fisher's exact test for biased strand distribution p-value = 1.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 246 total aligned reads displayed.

CCGCTGATTCGCCTGAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACGGGGGATATCCACTGTC  >  NC_000913/993106‑993400
                                                                                                                                                     |                                                                                                                                                 
ccGCTGATTCGCCTGAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCaa                                                                                                                                                   <  1:2695732/150‑1 (MQ=255)
 cGCTGATTCGCCTGAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAAt                                                                                                                                                  >  1:2161536/1‑150 (MQ=255)
 cGCTGATTCGCCTGAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAAt                                                                                                                                                  >  2:2147801/1‑150 (MQ=255)
 cGCTGATTCGCCTGAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAAt                                                                                                                                                  >  2:4007332/1‑150 (MQ=255)
  gCTGATTCGCCTGAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATa                                                                                                                                                 >  1:540199/1‑150 (MQ=255)
  gCTGATTCGCCTGAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATa                                                                                                                                                 >  1:1463653/1‑150 (MQ=255)
  gCTGATTCGCCTGAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATa                                                                                                                                                 >  1:180140/1‑150 (MQ=255)
  gCTGATTCGCCTGAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATa                                                                                                                                                 >  2:3078204/1‑150 (MQ=255)
      aTTCGCCTGAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAgcgc                                                                                                                                             >  2:3168124/1‑150 (MQ=255)
      aTTCGCCTGAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAgcgc                                                                                                                                             >  2:656518/1‑150 (MQ=255)
          gCCTGAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCGCTATTgccgcc                                                                                                                                         <  2:1608824/150‑1 (MQ=255)
          gCCTGAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCgccgcc                                                                                                                                         >  1:1003035/1‑150 (MQ=255)
           ccTGAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCa                                                                                                                                        <  1:3197198/150‑1 (MQ=255)
            cTGAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCGCTATTGCCGCCAt                                                                                                                                       <  1:1800337/150‑1 (MQ=255)
             tGAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATACATAACCGAATGGCGGCAATAGCGCCGCCAtt                                                                                                                                      >  1:2019416/1‑150 (MQ=255)
              taaaCGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCGCTATTGCCGCCATTc                                                                                                                                     <  1:1318181/149‑1 (MQ=255)
              gAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCGCTATTGCCGCCATTc                                                                                                                                     <  2:3909949/150‑1 (MQ=255)
              gAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTc                                                                                                                                     <  2:4057487/150‑1 (MQ=255)
              gAAACGTTACGAGGCCAACCGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTc                                                                                                                                     <  2:2388041/150‑1 (MQ=255)
                aaCGTTACGATGCCAAACGTCTGGATAAAATGCGCGCGGCGCTGGAACTTTTGAATGGGCTGTAAAATCTCTCTGGCGATCTGTACGAGAATATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCgg                                                                                                                                   <  1:2439876/150‑1 (MQ=255)
                aaCGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCgg                                                                                                                                   <  2:1055038/150‑1 (MQ=255)
                aaCGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCgg                                                                                                                                   <  2:3287430/150‑1 (MQ=255)
                      aCGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCGCTATTGCCGCCATTCggttattt                                                                                                                             <  1:1256529/150‑7 (MQ=39)
                             cAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGTAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCt                                                                                                                      <  1:3739776/150‑1 (MQ=255)
                             cAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCt                                                                                                                      <  2:341647/150‑1 (MQ=255)
                               aaCGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGt                                                                                                                    <  1:2670293/150‑1 (MQ=255)
                               aaCGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGt                                                                                                                    >  2:2184432/1‑150 (MQ=255)
                                    cAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCGGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTtct                                                                                                               <  1:1092476/150‑1 (MQ=255)
                                      ggAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGACTTTACCCCTGTTTTCTCa                                                                                                             >  2:564795/1‑150 (MQ=255)
                                      ggAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCa                                                                                                             <  1:836858/150‑1 (MQ=255)
                                       gagaAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAg                                                                                                            <  2:1884599/150‑1 (MQ=255)
                                       gagaAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAg                                                                                                            <  2:1144832/150‑1 (MQ=255)
                                        agaAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAgg                                                                                                           <  1:3145572/150‑1 (MQ=255)
                                         gaAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGc                                                                                                          >  1:719950/1‑150 (MQ=255)
                                         gaAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGc                                                                                                          >  2:3340596/1‑150 (MQ=255)
                                           aaaTGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGa                                                                                                        <  1:2746157/150‑1 (MQ=255)
                                           aaaTGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGa                                                                                                        <  2:3356567/150‑1 (MQ=255)
                                                cgcgcgGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTc                                                                                                   <  1:1902036/150‑1 (MQ=255)
                                                cgcgcgGCGCTGGAACAGTTGAAAGGGCTGGAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCa                                                                                                  <  1:1916811/150‑1 (MQ=255)
                                                   gcggcgCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGa                                                                                                <  2:344965/150‑1 (MQ=255)
                                                     ggcgCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGAtt                                                                                              >  2:1072313/1‑150 (MQ=255)
                                                      gcgcTGGAAGAGTTGACAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTc                                                                                             <  2:2886875/150‑1 (MQ=255)
                                                       cgcTGGAACAGTTGTAAGGGCTGGAAAATCTCTCGGGCGATCTGTACGATAAGATAACAAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTca                                                                                            <  1:410402/150‑1 (MQ=255)
                                                       cgcTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTca                                                                                            >  2:1313575/1‑150 (MQ=255)
                                                       cgcTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTca                                                                                            >  1:2200323/1‑150 (MQ=255)
                                                        gcTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGACTGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTcac                                                                                           <  1:3679190/150‑1 (MQ=255)
                                                        gcTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTcac                                                                                           <  1:3895037/150‑1 (MQ=255)
                                                          tGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTcacca                                                                                         <  2:2591858/150‑1 (MQ=255)
                                                          tGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTcacca                                                                                         <  2:471367/150‑1 (MQ=255)
                                                               cAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTg                                                                                    <  1:646137/150‑1 (MQ=255)
                                                                          gCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGtt                                                                         <  2:1234372/150‑1 (MQ=255)
                                                                          gCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGtt                                                                         <  1:622118/150‑1 (MQ=255)
                                                                           cTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTg                                                                        <  1:108263/150‑1 (MQ=255)
                                                                           cTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTg                                                                        >  2:1457898/1‑150 (MQ=255)
                                                                            tGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATACAGAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGACTTTACCCCTGTTTTCTCAGGCGAGTGTCAGATTCACCACGTTGCCTCACCCGTTGc                                                                       >  1:2034407/1‑150 (MQ=255)
                                                                            tGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGc                                                                       >  1:3996495/1‑150 (MQ=255)
                                                                            tGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGc                                                                       >  1:1417413/1‑150 (MQ=255)
                                                                            tGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGc                                                                       >  2:1705582/1‑150 (MQ=255)
                                                                              gAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCaa                                                                     <  2:148044/150‑1 (MQ=255)
                                                                              gAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCaa                                                                     <  1:2323347/150‑1 (MQ=255)
                                                                                  aTCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACAcc                                                                 <  1:337524/150‑1 (MQ=255)
                                                                                    ctctctGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTc                                                               <  1:2808916/150‑1 (MQ=255)
                                                                                     tctctGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTcc                                                              >  2:3762332/1‑150 (MQ=255)
                                                                                          ggCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTc                                                         <  1:2908535/150‑1 (MQ=255)
                                                                                          ggCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTc                                                         <  1:3600177/150‑1 (MQ=255)
                                                                                            cGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCa                                                       >  2:562180/1‑150 (MQ=255)
                                                                                                cTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTc                                                   >  2:4122200/1‑150 (MQ=255)
                                                                                                 tGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTcc                                                  >  1:3074677/1‑150 (MQ=255)
                                                                                                 tGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTcc                                                  >  2:2468156/1‑150 (MQ=255)
                                                                                                  gTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCg                                                 >  2:1308560/1‑150 (MQ=255)
                                                                                                      gagaAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTaa                                             >  1:2561248/1‑150 (MQ=255)
                                                                                                      gagaAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTaa                                             >  1:545179/1‑150 (MQ=255)
                                                                                                         aagaTAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCg                                          >  1:1032471/1‑150 (MQ=255)
                                                                                                          agaTAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGc                                         <  2:719950/150‑1 (MQ=255)
                                                                                                          agaTAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGc                                         <  1:3340596/150‑1 (MQ=255)
                                                                                                            aTAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCAc                                       >  1:1395441/1‑150 (MQ=255)
                                                                                                             tAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCAcc                                      <  1:2612388/150‑1 (MQ=255)
                                                                                                                cTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTGCCAGCTCCGCTAATCGCACCGAc                                   >  1:2275999/1‑150 (MQ=255)
                                                                                                                 tAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGAcc                                  <  2:2486918/150‑1 (MQ=255)
                                                                                                                 tAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGAcc                                  >  2:1670617/1‑150 (MQ=255)
                                                                                                                   aaGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCt                                >  2:3473722/1‑150 (MQ=255)
                                                                                                                     gCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTaa                              <  2:3538758/150‑1 (MQ=255)
                                                                                                                       aCTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATc                            <  2:2038601/150‑1 (MQ=255)
                                                                                                                         tGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGa                          >  1:294360/1‑150 (MQ=255)
                                                                                                                           gCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGAcg                        >  1:1773949/1‑150 (MQ=255)
                                                                                                                              ttataaataaCCGAATGGCGGCAATAGCGCCGCATTTCGGGTAATTTACCCCTGTTTTCTCAGGCTTGTTTAAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCgg                     <  1:1657576/148‑1 (MQ=255)
                                                                                                                                   aataaCCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTCATCGCACCGACCCTAATCGACGCGGACggg                >  1:2508211/1‑150 (MQ=255)
                                                                                                                                   aataaCCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACggg                >  1:442852/1‑150 (MQ=255)
                                                                                                                                   aataaCCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACggg                >  2:236684/1‑150 (MQ=255)
                                                                                                                                   aataaCCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACggg                <  2:3300654/150‑1 (MQ=255)
                                                                                                                                   aataaCCGAATGGCGGCAATAGCGCCGCATTTCGGGTAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGATAATCGCACCGACCCTAATCGACGCGGACggg                <  1:400839/150‑1 (MQ=255)
                                                                                                                                    ataaCCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACgggg               <  2:80507/150‑1 (MQ=255)
                                                                                                                                      aaCCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACGGGGGa             >  1:916974/1‑150 (MQ=255)
                                                                                                                                      aaCCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACGGGGGa             <  2:835572/150‑1 (MQ=255)
                                                                                                                                        ccGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGGTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCGCCGACCCTCTTCGACGCGGCCGGGGGata           >  2:3912765/1‑150 (MQ=255)
                                                                                                                                          gAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACGGGGGATATc         >  1:3946030/1‑150 (MQ=255)
                                                                                                                                          gAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACGGGGGATATc         >  2:2628351/1‑150 (MQ=255)
                                                                                                                                            aTGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTCTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACGGGGGATATCCa       <  1:1708863/150‑1 (MQ=255)
                                                                                                                                               gcggcAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACGGGGGATATCCACTg    >  2:404367/1‑150 (MQ=255)
                                                                                                                                                 ggcAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACGGGGGATATCCACTGTc  <  2:3692788/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
CCGCTGATTCGCCTGAAACGTTACGATGCCAAACGTCAGGAGAAAATGCGCGCGGCGCTGGAACAGTTGAAAGGGCTGGAAAATCTCTCTGGCGATCTGTACGAGAAGATAACTAAAGCACTGGCTTGATAAATAACCGAATGGCGGCAATAGCGCCGCCATTCGGGGAATTTACCCCTGTTTTCTCAGGCGAGTTTCAGATTCACCACGTTGCATCACCCGTTGCAACACCTCCGCTTCCAGCTCCGCTAATCGCACCGACCCTAATCGACGCGGACGGGGGATATCCACTGTC  >  NC_000913/993106‑993400

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: