Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,164,546 A→T 9.3% D151V (GAT→GTT)  nagZ → beta N‑acetyl‑glucosaminidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,164,5460AT 9.3% 718.8 / 9.0 236D151V (GAT→GTT) nagZbeta N‑acetyl‑glucosaminidase
Reads supporting (aligned to +/- strand):  ref base A (152/56);  new base T (3/25);  total (155/81)
Fisher's exact test for biased strand distribution p-value = 1.95e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 239 total aligned reads displayed.

TTGGTTGATGGCCAGCGAAATGATCGCTATGGATATTGATATCAGCTTTGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTAAAGATATGTCGGTCTTCAGTTCCTTA  >  NC_000913/1164397‑1164694
                                                                                                                                                     |                                                                                                                                                     
ttggttgATGGCCAGCGAAATGATCGCTATGGATATTGATATCAGCTTTGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGa                                                                                                                                                       >  1:20118/1‑150 (MQ=255)
ttggttgATGGCCAGCGAAATGATCGCTATGGATATTGATATCAGCTTTGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGa                                                                                                                                                       >  1:1856233/1‑150 (MQ=255)
 tggttgATGGCCAGCGAAATGATCGCTATGGATATTGATATCAGCTTTGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGAt                                                                                                                                                      <  1:2769890/150‑1 (MQ=255)
 tggttgATGGCCAGCGAAATGATCGCTATGGATATTGATATCAGCTTTGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGAt                                                                                                                                                      <  1:1228447/150‑1 (MQ=255)
              gCGAAATGATCGCTATGGATATTGATATCAGCTTTGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAg                                                                                                                                        >  2:1594802/1‑150 (MQ=255)
                   aTGATCGCTATGGATATTGATATCAGCTTTGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGa                                                                                                                                   <  2:4103866/150‑1 (MQ=255)
                    tGATCGCTATGGATATTGATATCAGCTTTGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAAGTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGaa                                                                                                                                  <  1:361923/150‑1 (MQ=255)
                     gATCGCTATGGATATTGATATCAGCTTTGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAAt                                                                                                                                 >  1:653716/1‑150 (MQ=255)
                       tCGCTATGGATATTGATATCAGCTTTGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTCTGCATGAAGCCGGAATGa                                                                                                                               >  2:3089782/1‑150 (MQ=255)
                       tCGCTATGGATATTGATATCAGCTTTGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGa                                                                                                                               >  2:1365349/1‑150 (MQ=255)
                        cGCTATGGATATTGATATCAGCTTTGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGaa                                                                                                                              <  1:4036773/150‑1 (MQ=255)
                         gCTATGGATATTGATATCAGCTTTGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGaaa                                                                                                                             <  2:2577503/150‑1 (MQ=255)
                         gCTATGGATATTGATATCAGCTTTGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGaaa                                                                                                                             >  1:3497579/1‑150 (MQ=255)
                         gCTATGGATATTGATATCAGCTTTGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGaaa                                                                                                                             <  2:4019638/150‑1 (MQ=255)
                         gCTATGGATATTGATATCAGCTTTGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGaaa                                                                                                                             >  1:76415/1‑150 (MQ=255)
                          cTATGGATATTGATATCAGCTTTGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGaaaa                                                                                                                            <  2:3371906/150‑1 (MQ=255)
                          cTATGGATATTGATATCAGCTTTGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGaaaa                                                                                                                            <  2:4039818/150‑1 (MQ=255)
                                     gATATCTGGTTTGCGCGTGTGCTGTTGGTGGGGCATAGCCGGGGGGCGATTGGCGAGCGTTCTTATCAGGCCGATCAACAAAAAGCCCGGGCAATGGCCAGCCGGTTTATGGTTGGTAGGCATGAAGCCGGATTGAAAACGACCGGGaaa                                                                                                                 <  2:3056985/150‑1 (MQ=255)
                                     gATATCAGCTTTGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGaaa                                                                                                                 >  2:2761289/1‑150 (MQ=255)
                                       tatCAGCTTTGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAATCCATGGCAATTGCCAGCCGGTTTCTTGATGGTATTCATGAAGCCGGAATGAAAACGACCGGGAAaca                                                                                                               >  1:1326337/1‑150 (MQ=255)
                                          cAGCTTTGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACtt                                                                                                            >  2:1617831/1‑150 (MQ=255)
                                           aGCTTTGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTc                                                                                                           >  2:1698692/1‑150 (MQ=255)
                                           aGCTTTGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGACCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTc                                                                                                           >  2:591046/1‑150 (MQ=255)
                                                      tgtgCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGAGGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACgg                                                                                                >  2:3326344/1‑150 (MQ=255)
                                                          gtGGTTGTCGGGCATATCCGCGCGGCGTTTGGCGAGCGTTCTTATCATGCCGATCCACAAAACGCCCTGGCAATTGCCAGCGGGTTTATTGATGGTATGAATGACGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCa                                                                                            <  2:3022694/149‑1 (MQ=255)
                                                          cTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCa                                                                                            >  1:2068800/1‑150 (MQ=255)
                                                          cTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCa                                                                                            >  1:1955522/1‑150 (MQ=255)
                                                          cTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCa                                                                                            >  1:2615630/1‑150 (MQ=255)
                                                           tGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAg                                                                                           >  1:3070968/1‑150 (MQ=255)
                                                           tGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATAGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACCCGGTGCAg                                                                                           >  2:3406124/1‑150 (MQ=255)
                                                                 tggggattatCCGCGCGGCTTTTGGCTGGCTTTCTTATCATGCCTATCAAAAAAACGCCCTGTAATTTGCCAGCGGTTTTTTTTTTGGTATGCTTGACGCGGGAATCAACACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACgg                                                                                     <  2:719990/143‑1 (MQ=255)
                                                                 tCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACgg                                                                                     >  2:2595051/1‑150 (MQ=255)
                                                                 tCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACgg                                                                                     >  2:3594183/1‑150 (MQ=255)
                                                                 tCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACgg                                                                                     >  1:2372274/1‑150 (MQ=255)
                                                                 tCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGAAATTGCCAGCCGGTTTATTGTTGGTATGCATGACGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACgg                                                                                     <  2:667237/150‑1 (MQ=255)
                                                                     gCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGa                                                                                 >  1:3360948/1‑150 (MQ=255)
                                                                       atatCCGCGGGGCGCGGGGGGAGCGTTCTTATCATGCCGACCCACAAAACGCCCTGGCATTGGCCCGCGGGTTTATTTATGGTATGCATGACGCCGGAATGAACACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACt                                                                               <  2:2050749/150‑1 (MQ=255)
                                                                          tCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGGCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTcac                                                                            >  1:1245707/1‑150 (MQ=255)
                                                                               gggggttttGGCGAGGTTTCTTATCTTGCCTATCCAAAAAATGCCCTGGATTTTGCCAGCGGGTTTTTTGTTGTTTTTCATTATGCGGTATTCAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACaaa                                                                       <  2:495229/143‑1 (MQ=255)
                                                                               gcggctTTTGGCGCGCGTTATTACCAGGCCGACCCAAAAAAACCCCTGGAATTTGCCAGCCGGTTTATTGATGTTATGCATTAAGCGGTAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACaaa                                                                       <  2:527454/150‑1 (MQ=255)
                                                                               gcggctTTTGGCGAGCGTTCTTATCATGCCTATCCACAAAAAGCCCTGGAAATTGCCAGCGGGTTTATTGTTGGTATGCATGAAGCCGTAATTAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACaaa                                                                       <  2:2035827/150‑1 (MQ=255)
                                                                               gcggcgATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACaaa                                                                       >  2:388629/1‑150 (MQ=255)
                                                                                cggcgATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAg                                                                      >  2:2849486/1‑150 (MQ=255)
                                                                                    gATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAac                                                                  >  1:3520372/1‑150 (MQ=255)
                                                                                       tGGCGTGCTTTCTTATCATGCCGATCCACAAAAAGCCGTGGCATTTGCCAGCCGGTTTTTTGTTGTTTTGCATGATGCCGGATTGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACAcc                                                               <  2:4133145/150‑1 (MQ=255)
                                                                                         gcgtgggTTCTTATCATGCCTATCCACAAAAAGCCCTGGCATTTGCCTGCCGTTTTTTTGTTGTTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGt                                                             <  2:2232849/144‑1 (MQ=255)
                                                                                         ccgcgCGTTCTCACCATCCCCAACCCCAAAACCCCCTGCCATTCCCCCGCCGTTTTATGTTTTTATTCAATGATGCCGCATTCAAAACGACCGGGAAACACTTCCCAGGACTCGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGt                                                             <  1:4181953/146‑1 (MQ=255)
                                                                                           gAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGc                                                           >  2:3122831/1‑150 (MQ=255)
                                                                                           gAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGc                                                           >  2:265572/1‑150 (MQ=255)
                                                                                            aGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCg                                                          >  2:3757590/1‑150 (MQ=255)
                                                                                            aGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCg                                                          >  2:1440734/1‑150 (MQ=255)
                                                                                               gTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATc                                                       >  1:3106224/1‑150 (MQ=255)
                                                                                                ttCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATcc                                                      >  2:3644607/1‑150 (MQ=255)
                                                                                                 tCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCa                                                     >  1:1674248/1‑150 (MQ=255)
                                                                                                   ttATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACg                                                   >  2:96341/1‑150 (MQ=255)
                                                                                                     aTCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTc                                                 <  2:3497557/150‑1 (MQ=255)
                                                                                                      tCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTcc                                                >  1:2987426/1‑150 (MQ=255)
                                                                                                       cATGCCTATCCACAAAACGCCCTGGAATTTGCCAGCCGTTTTTTTGTTGGTTTGATTTAAGCCGTAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCca                                               <  1:2447511/150‑1 (MQ=255)
                                                                                                        aTGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCcac                                              >  1:3075872/1‑150 (MQ=255)
                                                                                                        aTGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCcac                                              >  1:2131950/1‑150 (MQ=255)
                                                                                                         tGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCcaca                                             >  2:1161492/1‑150 (MQ=255)
                                                                                                         tGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCcaca                                             >  2:102349/1‑150 (MQ=255)
                                                                                                          gCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACaa                                            >  2:778707/1‑150 (MQ=255)
                                                                                                          gCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACaa                                            >  2:778964/1‑150 (MQ=255)
                                                                                                            cGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGc                                          >  1:3749391/1‑150 (MQ=255)
                                                                                                              aTCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCgg                                        >  1:446507/1‑150 (MQ=255)
                                                                                                               tCCACAAAAAGCCCTGGAATTGGCCAGCCGTTTTTTTTTTGTTTTGATTGACGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGga                                       <  1:3019931/150‑1 (MQ=255)
                                                                                                                  acaAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACACGCGGAGAt                                    >  1:575601/1‑150 (MQ=255)
                                                                                                                   caAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGACGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGAtt                                   <  2:3508868/150‑1 (MQ=255)
                                                                                                                    aaaaaGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTc                                  >  1:3237384/1‑150 (MQ=255)
                                                                                                                      aaaGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTcgc                                >  1:1090518/1‑150 (MQ=255)
                                                                                                                         gCCCTGGCAATGGCCAGCCGTTTTATTGATGGTATGCATGAAGCGGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCt                             <  2:2342388/150‑1 (MQ=255)
                                                                                                                         ccccTGCCAATTGCCCGCCGGTTTATTGATGTTAGGCCTGACGCCGCAATCAACACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCt                             <  2:365153/149‑1 (MQ=255)
                                                                                                                            ctgtaatttGCCCGCGGTTTTTTTGATTGATTGCATGACGCGGTAATCAACACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTaaa                          <  1:847398/143‑1 (MQ=255)
                                                                                                                            cTGGCATTTGCCTGCGTTTTTTTTGTTGTTTTGCATTACGCGGGATTTAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTaaa                          <  2:63006/150‑1 (MQ=255)
                                                                                                                            cTGGCATTTGCCAGCCGTTTTTTTGTTGGTAGGCATGAAGCCGTAATCAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTaaa                          <  1:846933/150‑1 (MQ=255)
                                                                                                                            cTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTaaa                          >  2:3496085/1‑150 (MQ=255)
                                                                                                                            cTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTaaa                          >  2:1327916/1‑150 (MQ=255)
                                                                                                                            cTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTaaa                          >  1:955761/1‑150 (MQ=255)
                                                                                                                            cTGGAATTTGCATGCCGTTTTATTTATGTTTTGAATTAAGCCGCAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTaaa                          <  2:2539851/150‑1 (MQ=255)
                                                                                                                             tGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTAAAg                         >  1:3753230/1‑150 (MQ=255)
                                                                                                                             tGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTAAAg                         >  2:3946648/1‑150 (MQ=255)
                                                                                                                             tGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTAAAg                         >  1:1179346/1‑150 (MQ=255)
                                                                                                                              ggCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTAAAGa                        >  2:2716314/1‑150 (MQ=255)
                                                                                                                               gCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTAAAGat                       >  1:2438238/1‑150 (MQ=255)
                                                                                                                                 aaTTGCCCGCGGGTTTTTTGTTGTTATGAATTATGCCGTAATTAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTAAAGatat                     <  2:1211515/150‑1 (MQ=255)
                                                                                                                                     gCCTGCGGGTTTATTGTTGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTAAAGATATGTCg                 <  2:1043722/150‑1 (MQ=255)
                                                                                                                                       cAGCGGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTAAAGATATGTCGGt               <  2:3113886/150‑1 (MQ=255)
                                                                                                                                       cAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTAAAGATATGTCGGt               >  2:2361298/1‑150 (MQ=255)
                                                                                                                                        aGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTAAAGATATGTCGGTc              >  2:1827745/1‑150 (MQ=255)
                                                                                                                                        aGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTAAAGATATGTCGGTc              >  2:82392/1‑150 (MQ=255)
                                                                                                                                        aGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTAAAGATATGTCGGTc              >  2:1148618/1‑150 (MQ=255)
                                                                                                                                        aGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTAAAGATATGTCGGTc              >  1:3863256/1‑150 (MQ=255)
                                                                                                                                         gCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTAAAGATATGTCGGTCt             >  1:1473961/1‑150 (MQ=255)
                                                                                                                                             gTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTAAAGATATGTCGGTCTTCAg         >  2:1947811/1‑150 (MQ=255)
                                                                                                                                               ttATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTAAAGATATGTCGGTCTTCAGtt       >  1:292045/1‑150 (MQ=255)
                                                                                                                                                  ttGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTAAAGATATGTCGGTCTTCAGTTCCt    >  1:1713762/1‑150 (MQ=255)
                                                                                                                                                  ttGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTAAAGATATGTCGGTCTTCAGTTCCt    >  2:2355514/1‑150 (MQ=255)
                                                                                                                                                    gATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTAAAGATATGTCGGTCTTCAGTTCCTTa  >  1:3662048/1‑150 (MQ=255)
                                                                                                                                                    gATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTAAAGATATGTCGGTCTTCAGTTCCTTa  >  2:2178746/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTGGTTGATGGCCAGCGAAATGATCGCTATGGATATTGATATCAGCTTTGCGCCTGTGCTGGATGTCGGGCATATCAGCGCGGCGATTGGCGAGCGTTCTTATCATGCCGATCCACAAAAAGCCCTGGCAATTGCCAGCCGGTTTATTGATGGTATGCATGAAGCCGGAATGAAAACGACCGGGAAACACTTCCCAGGACACGGTGCAGTAACGGCAGACTCACACAAAGAAACACCGTGCGATCCACGTCCACAAGCGGAGATTCGCGCTAAAGATATGTCGGTCTTCAGTTCCTTA  >  NC_000913/1164397‑1164694

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: