Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,314,682 A→T 5.6% intergenic (+24/+36) ompW → / ← yciE outer membrane protein W/putative rubrerythrin/ferritin‑like metal‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,314,6820AT 5.6% 742.0 / 25.6 212intergenic (+24/+36)ompW/yciEouter membrane protein W/putative rubrerythrin/ferritin‑like metal‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (115/84);  new base T (10/2);  total (126/86)
Fisher's exact test for biased strand distribution p-value = 1.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 217 total aligned reads displayed.

AACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTAAAATCGC  >  NC_000913/1314533‑1314831
                                                                                                                                                     |                                                                                                                                                     
aaCATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTaa                                                                                                                                                       >  2:1329858/1‑150 (MQ=255)
 aCATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAat                                                                                                                                                      >  1:196777/1‑150 (MQ=255)
  cATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTActa                                                                                                                                                     >  1:3939727/1‑150 (MQ=255)
    tGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAAtata                                                                                                                                                   >  1:2184511/1‑150 (MQ=255)
      tCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACg                                                                                                                                                 >  1:2578923/1‑150 (MQ=255)
        aGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATCTACggg                                                                                                                                               >  1:1428541/1‑150 (MQ=255)
                aCATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGTTCGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATaa                                                                                                                                       <  2:3070662/150‑1 (MQ=255)
                aCATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATac                                                                                                                                       >  1:3860136/1‑149 (MQ=255)
                aCATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATaa                                                                                                                                       >  1:4152118/1‑150 (MQ=255)
                aCATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATaa                                                                                                                                       >  1:1701094/1‑150 (MQ=255)
                aCATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATaa                                                                                                                                       >  1:161439/1‑150 (MQ=255)
                aCATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATaa                                                                                                                                       >  1:848156/1‑150 (MQ=255)
                 cATGGATAGCGATACCACCGCCAATTTTAAGCTGGGCGGTGCACTGCAACACGATTGCGTACGCCTCTTTGCGTGGGTGTTTATGTTCTCAGCCGGTTATCGTTTTTAATTCCGCACAAACACGACCCCGTAATATACGGGGTCAATAAg                                                                                                                                      <  2:3911799/150‑1 (MQ=255)
                        atCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATgg                                                                                                                               >  2:111236/1‑150 (MQ=255)
                         tCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGt                                                                                                                              >  2:2327874/1‑150 (MQ=255)
                         tCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGt                                                                                                                              >  1:3292198/1‑150 (MQ=255)
                          cGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCGGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGta                                                                                                                             <  2:4100559/150‑1 (MQ=255)
                          cGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGACAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGta                                                                                                                             <  2:461275/150‑1 (MQ=255)
                            aTACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACAGGGtata                                                                                                                           >  1:551314/1‑150 (MQ=255)
                                   cGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGta                                                                                                                    >  1:1908492/1‑150 (MQ=255)
                                    gCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAATCGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGtat                                                                                                                   >  1:1680829/1‑150 (MQ=255)
                                    gCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGAGCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGtat                                                                                                                   <  2:1464901/150‑1 (MQ=255)
                                    gCCAATTATAAGCTGGGCGGAGCACAGCAACACGATAGCGAACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGATTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCACTAAGGACATGGTATAAAGGGtat                                                                                                                   >  1:4094900/1‑150 (MQ=255)
                                     ccAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGtatt                                                                                                                  <  2:286952/150‑1 (MQ=255)
                                              aGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTCTGTTCTCAGCAGGATATCGTTTTAAATTCCGCACAAAAACGACCCCGTACTATACGGGGTCAATAAGGACATGGCATAAAGGGTATTATTTCTTCg                                                                                                         >  2:3665138/1‑150 (MQ=255)
                                               gCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGc                                                                                                        >  2:200576/1‑150 (MQ=255)
                                               gCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGc                                                                                                        >  1:240033/1‑150 (MQ=255)
                                                   ggCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCt                                                                                                    >  1:1363891/1‑150 (MQ=255)
                                                   ggCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCt                                                                                                    >  1:1852196/1‑150 (MQ=255)
                                                    ggggTGCCCAGCAACACTATAGCGTACGCATCGATCCGTGGGTTTTTTTGTTCTCAGCTGTATATCTTTTTTATTTCCGCAAAAACACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTa                                                                                                   <  1:1201299/148‑1 (MQ=255)
                                                    gCGGTGCACAGCAACCCGATAGCGTACGCCTCGAGCCGTGGGTGTTTATGTTCTCAGCAGTATATCTTTTTTATTTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTa                                                                                                   <  1:2646213/150‑1 (MQ=255)
                                                    gCGGTGCACAGAAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTa                                                                                                   <  2:3231063/150‑1 (MQ=255)
                                                      ggTGCACAGCAACTCGATAGCGTACGCCTCGATCCGTGGGTGTGTTTGTTCTCAGCAGGATATCGTTTTTAATTCCGCAAAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACg                                                                                                 <  2:3718978/150‑1 (MQ=255)
                                                      ggTGCACAGCAACCGGATAGCGTACGCCTGGATCCGTGGGTGTTTTTGTTCTGTGCTGGATATCGTTTTTAAGTCCGCACAAAAACGACCCCGTAATCTACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACg                                                                                                 <  2:1357651/150‑1 (MQ=255)
                                                         gCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATTTCGTTTTTAATTCCGCACAAACACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCa                                                                                              <  2:1626112/150‑1 (MQ=255)
                                                          cacaGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCAt                                                                                             >  1:3029581/1‑150 (MQ=255)
                                                          cacaGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCAt                                                                                             <  2:3764234/150‑1 (MQ=255)
                                                            caGCAACACGATAGCGTACGCCGCGATCCGGGGGTGTTTATGTTCTCAGCCGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCa                                                                                           <  2:1891214/150‑1 (MQ=255)
                                                                aaCACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGttt                                                                                       >  1:382044/1‑150 (MQ=255)
                                                                 acacGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTc                                                                                      >  1:3755406/1‑150 (MQ=255)
                                                                  cacGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCa                                                                                     <  2:2686623/150‑1 (MQ=255)
                                                                    cGATAGCGTACGCCTCGATCGGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAACAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCaga                                                                                   <  2:3188476/150‑1 (MQ=255)
                                                                    cGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCaga                                                                                   <  2:2101784/150‑1 (MQ=255)
                                                                     gATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCagag                                                                                  >  1:3872746/1‑150 (MQ=255)
                                                                     gATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCagag                                                                                  >  1:2909297/1‑150 (MQ=255)
                                                                              cGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTaaa                                                                         <  2:1852196/150‑1 (MQ=255)
                                                                               gCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAACACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTaaaa                                                                        <  1:2746602/150‑1 (MQ=255)
                                                                                 cTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAAt                                                                      >  2:1688465/1‑150 (MQ=255)
                                                                                    gATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTtc                                                                   >  1:3580364/1‑150 (MQ=255)
                                                                                     aTCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTtct                                                                  >  1:1791278/1‑150 (MQ=255)
                                                                                            ggTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATATCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAgttgt                                                           >  2:3927925/1‑150 (MQ=255)
                                                                                             gtgtTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAgtggtt                                                          >  1:2454746/1‑150 (MQ=255)
                                                                                              tgtTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGttt                                                         >  2:1020275/1‑150 (MQ=255)
                                                                                              tgtTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGttt                                                         >  1:2225570/1‑150 (MQ=255)
                                                                                              tgtTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGttt                                                         >  1:3571764/1‑150 (MQ=255)
                                                                                                tttATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGc                                                       >  1:1100157/1‑150 (MQ=255)
                                                                                                tttATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGc                                                       >  1:4063627/1‑150 (MQ=255)
                                                                                                  taagTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTACGCTTCTACGCCATCAGTTTCAGAGCGAATAAAAAATTTCTCAGTTGTTTGCgg                                                     >  2:1227103/4‑150 (MQ=255)
                                                                                                  tATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCgg                                                     >  2:1813327/1‑150 (MQ=255)
                                                                                                        ctcAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATAtt                                               >  2:1046429/1‑150 (MQ=255)
                                                                                                        ctcAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATAtt                                               >  2:2659932/1‑150 (MQ=255)
                                                                                                         tcAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTc                                              >  2:1740500/1‑150 (MQ=255)
                                                                                                           agcagGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTg                                            >  2:1318862/1‑150 (MQ=255)
                                                                                                                gATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCTGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCa                                       >  2:1643069/1‑150 (MQ=255)
                                                                                                                  tatCTTTTTTAAGACCGCACAAACACGACCCCGTAAGATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGc                                     <  2:2593266/150‑1 (MQ=255)
                                                                                                                   atCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGcc                                    >  2:311641/1‑150 (MQ=255)
                                                                                                                   atCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTGGCGGAATATTCTGAATCAGcc                                    <  2:2411256/150‑1 (MQ=255)
                                                                                                                    tCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCa                                   <  2:3292198/150‑1 (MQ=255)
                                                                                                                      gTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTTTTTCTTCGCTTCTACGCCTTGAGTTTCAGAGCGAATTTATAATTTGTGTGTTGTTTGCGGAATATTCTGTTTCTGCCAGt                                 >  2:2229504/1‑150 (MQ=255)
                                                                                                                      gTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGt                                 >  2:3451685/1‑150 (MQ=255)
                                                                                                                       tttttAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTc                                >  2:1746180/1‑150 (MQ=255)
                                                                                                                       tttttAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTc                                >  2:592993/1‑150 (MQ=255)
                                                                                                                       tttttAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTc                                >  2:250701/1‑150 (MQ=255)
                                                                                                                       tttttAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACACCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTc                                >  1:2026099/1‑150 (MQ=255)
                                                                                                                       ttttaAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATCTGGACATGGGCGAAAGGGTATTCTTTCTTCGCTTCGACGCCATCAGTTTCAGAGCGAAATAAAAATTGCCCAGTGGTTTGCGGAATATTCTGAATCAGCCAggc                                >  2:178749/1‑148 (MQ=255)
                                                                                                                               tCCGCACAAACACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCAttt                        <  1:1278172/150‑1 (MQ=255)
                                                                                                                                  gCACAAAAACGACCCCGTATATTACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCt                     <  2:1298343/150‑1 (MQ=255)
                                                                                                                                  gCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCt                     >  2:418287/1‑150 (MQ=255)
                                                                                                                                   cacaAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCtt                    >  2:2128491/1‑150 (MQ=255)
                                                                                                                                   cacaAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCtt                    >  2:3254446/1‑150 (MQ=255)
                                                                                                                                    acaAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTGAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCttt                   >  1:2300128/1‑150 (MQ=255)
                                                                                                                                    acaAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCttt                   >  1:495779/1‑150 (MQ=255)
                                                                                                                                    acaAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCttt                   <  1:28287/150‑1 (MQ=255)
                                                                                                                                    acaAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCttt                   >  2:1838627/1‑150 (MQ=255)
                                                                                                                                    acaAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCttt                   <  1:3392674/150‑1 (MQ=255)
                                                                                                                                     caAAAACGACCCCGTAATATACGGGGTCAATACGGACATGGGATAATGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAGATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCtttt                  >  2:2718747/1‑150 (MQ=255)
                                                                                                                                      aaccaCGACCCCGTCATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTc                 <  1:3516699/146‑1 (MQ=255)
                                                                                                                                      aaaaaCGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTc                 <  1:2944127/150‑1 (MQ=255)
                                                                                                                                       ttattgACCCCGTATATTACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTcc                >  1:2019373/6‑150 (MQ=255)
                                                                                                                                       ttattgACCCCGTATATTACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTAGCTTCTACTCCATAAGTTTCATAGCGAATTAAAAATTTATCAGTTGTTTGAGGAAAATACTGAATCAGCCAGTCGGCCATTTGCTTTTcc                >  1:1567107/6‑150 (MQ=255)
                                                                                                                                       aaaaCGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTcc                <  2:770063/150‑1 (MQ=255)
                                                                                                                                       aaaaCGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTcc                <  1:3694428/150‑1 (MQ=255)
                                                                                                                                        aaaCGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCt               >  1:1003642/1‑150 (MQ=255)
                                                                                                                                          ttgACCCCGTATATTACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTGCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCa             >  1:1675457/3‑150 (MQ=255)
                                                                                                                                                ccGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTaaa       <  2:1027391/150‑1 (MQ=255)
                                                                                                                                                ccGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTaaa       >  1:1637254/1‑150 (MQ=255)
                                                                                                                                                 cGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTaaaa      >  2:1939698/1‑150 (MQ=255)
                                                                                                                                                  gTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTAAAAt     <  1:4184032/150‑1 (MQ=255)
                                                                                                                                                   tAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTAAAATc    >  2:1085837/1‑150 (MQ=255)
                                                                                                                                                     atatACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTAAAATCGc  <  1:2307139/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTAAAATCGC  >  NC_000913/1314533‑1314831

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: