Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,314,683 T→A 5.5% intergenic (+25/+35) ompW → / ← yciE outer membrane protein W/putative rubrerythrin/ferritin‑like metal‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,314,6830TA 5.5% 751.6 / 20.7 210intergenic (+25/+35)ompW/yciEouter membrane protein W/putative rubrerythrin/ferritin‑like metal‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (114/82);  new base A (10/2);  total (124/86)
Fisher's exact test for biased strand distribution p-value = 1.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 217 total aligned reads displayed.

ACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTAAAATCGCT  >  NC_000913/1314534‑1314832
                                                                                                                                                     |                                                                                                                                                     
aCATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAat                                                                                                                                                       >  1:196777/1‑150 (MQ=255)
   tGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAAtata                                                                                                                                                    >  1:2184511/1‑150 (MQ=255)
     tCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACg                                                                                                                                                  >  1:4130421/1‑150 (MQ=255)
     tCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACg                                                                                                                                                  >  1:2578923/1‑150 (MQ=255)
       aGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATCTACggg                                                                                                                                                >  1:1428541/1‑150 (MQ=255)
        gtgtGGTACATGGATATCGATACCACCGCCAATTCTAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACgggg                                                                                                                                               <  2:126512/150‑1 (MQ=255)
               aCATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGTTCGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATaa                                                                                                                                        <  2:3070662/150‑1 (MQ=255)
               aCATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATaa                                                                                                                                        >  1:307754/1‑150 (MQ=255)
               aCATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATaa                                                                                                                                        >  1:1701094/1‑150 (MQ=255)
               aCATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATaa                                                                                                                                        >  1:161439/1‑150 (MQ=255)
                cATGGATAGCGATACCACCGCCAATTTTAAGCTGGGCGGTGCACTGCAACACGATTGCGTACGCCTCTTTGCGTGGGTGTTTATGTTCTCAGCCGGTTATCGTTTTTAATTCCGCACAAACACGACCCCGTAATATACGGGGTCAATAAg                                                                                                                                       <  2:3911799/150‑1 (MQ=255)
                        tCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGt                                                                                                                               >  2:2327874/1‑150 (MQ=255)
                        tCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGt                                                                                                                               >  1:443160/1‑150 (MQ=255)
                         cGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCGGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGta                                                                                                                              <  2:4100559/150‑1 (MQ=255)
                         cGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGACAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGta                                                                                                                              <  2:461275/150‑1 (MQ=255)
                           aTACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGtata                                                                                                                            >  1:718207/1‑150 (MQ=255)
                                  cGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGta                                                                                                                     >  1:1908492/1‑150 (MQ=255)
                                   gCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAATCGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGtat                                                                                                                    >  1:1680829/1‑150 (MQ=255)
                                   gCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGtat                                                                                                                    >  1:3693132/1‑150 (MQ=255)
                                   gCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGtat                                                                                                                    >  1:2922306/1‑150 (MQ=255)
                                   gCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGAGCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGtat                                                                                                                    <  2:1464901/150‑1 (MQ=255)
                                    ccAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGtatt                                                                                                                   <  2:286952/150‑1 (MQ=255)
                                             aGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTCTGTTCTCAGCAGGATATCGTTTTAAATTCCGCACAAAAACGACCCCGTACTATACGGGGTCAATAAGGACATGGCATAAAGGGTATTATTTCTTCg                                                                                                          >  2:3665138/1‑150 (MQ=255)
                                              gCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGc                                                                                                         >  2:200576/1‑150 (MQ=255)
                                              gCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGc                                                                                                         >  1:240033/1‑150 (MQ=255)
                                                  ggCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCt                                                                                                     >  1:1363891/1‑150 (MQ=255)
                                                  ggCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCt                                                                                                     >  1:1852196/1‑150 (MQ=255)
                                                   ggggTGCCCAGCAACACTATAGCGTACGCATCGATCCGTGGGTTTTTTTGTTCTCAGCTGTATATCTTTTTTATTTCCGCAAAAACACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTa                                                                                                    <  1:1201299/148‑1 (MQ=255)
                                                   gCGGTGCACAGCAACCCGATAGCGTACGCCTCGAGCCGTGGGTGTTTATGTTCTCAGCAGTATATCTTTTTTATTTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTa                                                                                                    <  1:2646213/150‑1 (MQ=255)
                                                   gCGGTGCACAGAAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTa                                                                                                    <  2:3231063/150‑1 (MQ=255)
                                                     ggTGCACAGCAACTCGATAGCGTACGCCTCGATCCGTGGGTGTGTTTGTTCTCAGCAGGATATCGTTTTTAATTCCGCAAAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACg                                                                                                  <  2:3718978/150‑1 (MQ=255)
                                                     ggTGCACAGCAACCGGATAGCGTACGCCTGGATCCGTGGGTGTTTTTGTTCTGTGCTGGATATCGTTTTTAAGTCCGCACAAAAACGACCCCGTAATCTACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACg                                                                                                  <  2:1357651/150‑1 (MQ=255)
                                                        gCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATTTCGTTTTTAATTCCGCACAAACACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCa                                                                                               <  2:1626112/150‑1 (MQ=255)
                                                         cacaGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCAt                                                                                              <  2:3764234/150‑1 (MQ=255)
                                                         cacaGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCAt                                                                                              >  1:4037444/1‑150 (MQ=255)
                                                           caGCAACACGATAGCGTACGCCGCGATCCGGGGGTGTTTATGTTCTCAGCCGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCa                                                                                            <  2:1891214/150‑1 (MQ=255)
                                                               aaCACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGttt                                                                                        >  1:3539143/1‑150 (MQ=255)
                                                                acacGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTc                                                                                       >  1:521987/1‑150 (MQ=255)
                                                                 cacGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCa                                                                                      <  2:2686623/150‑1 (MQ=255)
                                                                   cGATAGCGTACGCCTCGATCGGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAACAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCaga                                                                                    <  2:3188476/150‑1 (MQ=255)
                                                                   cGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCaga                                                                                    <  2:2101784/150‑1 (MQ=255)
                                                                    gATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCAAAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCagag                                                                                   <  1:2817407/150‑1 (MQ=255)
                                                                         cGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAAt                                                                              <  1:3853811/150‑1 (MQ=255)
                                                                             cGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTaaa                                                                          <  2:1852196/150‑1 (MQ=255)
                                                                              gCCTCGATCGGTGGGTGTTTCTGTTCTCCGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTaaaa                                                                         <  1:3902628/150‑1 (MQ=255)
                                                                              gCCTCGATCCGTGTGTGTTTATGTTCTCCGCAGTAAATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTaaaa                                                                         <  1:2885875/150‑1 (MQ=255)
                                                                              gCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCTTTTTTAATTCCGCACAAACACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTaaaa                                                                         <  1:376438/150‑1 (MQ=255)
                                                                              gCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAACACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTaaaa                                                                         <  1:2746602/150‑1 (MQ=255)
                                                                                cTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAAt                                                                       >  2:1688465/1‑150 (MQ=255)
                                                                                cTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAAt                                                                       >  1:3023445/1‑150 (MQ=255)
                                                                                    aTCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTtct                                                                   >  1:1791278/1‑150 (MQ=255)
                                                                                           ggTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAgttgt                                                            >  2:1185475/1‑150 (MQ=255)
                                                                                           ggTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATATCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAgttgt                                                            >  2:3927925/1‑150 (MQ=255)
                                                                                            gtgtTTATGTTCTCAGCCGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAgttgtt                                                           <  2:1101224/150‑1 (MQ=255)
                                                                                            gtgtTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAgtggtt                                                           >  1:2454746/1‑150 (MQ=255)
                                                                                            gtgtTTATGTTCTCAGCAGGATATCGTTTTTAAGTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAgttgtt                                                           <  1:4083926/150‑1 (MQ=255)
                                                                                             tgtTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGttt                                                          >  1:2225570/1‑150 (MQ=255)
                                                                                               tttATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGc                                                        >  1:1100157/1‑150 (MQ=255)
                                                                                               tttATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGc                                                        >  1:372600/1‑150 (MQ=255)
                                                                                                 tATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCgg                                                      >  2:1813327/1‑150 (MQ=255)
                                                                                                 tATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCgg                                                      >  1:3867886/1‑150 (MQ=255)
                                                                                                       ctcAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATAtt                                                >  2:2659932/1‑150 (MQ=255)
                                                                                                        tcAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTc                                               >  2:1740500/1‑150 (MQ=255)
                                                                                                        tcAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTc                                               <  2:1326934/150‑1 (MQ=255)
                                                                                                          agcagGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTg                                             >  2:1318725/1‑150 (MQ=255)
                                                                                                             agGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAAt                                          <  2:1067553/150‑1 (MQ=255)
                                                                                                               gATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCTGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCa                                        >  2:1643069/1‑150 (MQ=255)
                                                                                                                 tatCTTTTTTAAGACCGCACAAACACGACCCCGTAAGATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGc                                      <  2:2593266/150‑1 (MQ=255)
                                                                                                                  atCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGcc                                     >  2:311641/1‑150 (MQ=255)
                                                                                                                  atCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTGGCGGAATATTCTGAATCAGcc                                     <  2:2411256/150‑1 (MQ=255)
                                                                                                                   tCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCa                                    <  2:3292198/150‑1 (MQ=255)
                                                                                                                     gTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTTTTTCTTCGCTTCTACGCCTTGAGTTTCAGAGCGAATTTATAATTTGTGTGTTGTTTGCGGAATATTCTGTTTCTGCCAGt                                  >  2:2229504/1‑150 (MQ=255)
                                                                                                                     gTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGt                                  >  2:3451685/1‑150 (MQ=255)
                                                                                                                      tttttAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTc                                 >  2:1746180/1‑150 (MQ=255)
                                                                                                                      tttttAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTc                                 >  2:592993/1‑150 (MQ=255)
                                                                                                                      tttttAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTc                                 >  2:250701/1‑150 (MQ=255)
                                                                                                                      tttttAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTc                                 >  2:1028617/1‑150 (MQ=255)
                                                                                                                      tttttAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACACCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTc                                 >  1:2026099/1‑150 (MQ=255)
                                                                                                                      ttttaAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATCTGGACATGGGCGAAAGGGTATTCTTTCTTCGCTTCGACGCCATCAGTTTCAGAGCGAAATAAAAATTGCCCAGTGGTTTGCGGAATATTCTGAATCAGCCAggc                                 >  2:178749/1‑148 (MQ=255)
                                                                                                                              tCCGCACAAACACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCAttt                         <  1:1278172/150‑1 (MQ=255)
                                                                                                                                 gCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCt                      >  2:418287/1‑150 (MQ=255)
                                                                                                                                  cacaAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCtt                     >  2:2128491/1‑150 (MQ=255)
                                                                                                                                  cacaAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCtt                     >  2:3254446/1‑150 (MQ=255)
                                                                                                                                   acaAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTGAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCttt                    >  1:2300128/1‑150 (MQ=255)
                                                                                                                                   acaAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAATATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCttt                    >  2:1003680/1‑150 (MQ=255)
                                                                                                                                   acaAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCttt                    <  1:3366758/150‑1 (MQ=255)
                                                                                                                                   acaAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCttt                    >  2:1838627/1‑150 (MQ=255)
                                                                                                                                   acaAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCttt                    <  1:3392696/150‑1 (MQ=255)
                                                                                                                                    caAAAACGACCCCGTAATATACGGGGTCAATACGGACATGGGATAATGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAGATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCtttt                   >  2:2718747/1‑150 (MQ=255)
                                                                                                                                      ttattgACCCCGTATATTACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTcc                 >  1:2019373/6‑150 (MQ=255)
                                                                                                                                      ttattgACCCCGTATATTACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTAGCTTCTACTCCATAAGTTTCATAGCGAATTAAAAATTTATCAGTTGTTTGAGGAAAATACTGAATCAGCCAGTCGGCCATTTGCTTTTcc                 >  1:1567107/6‑150 (MQ=255)
                                                                                                                                      aaaaCGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTcc                 <  2:770063/150‑1 (MQ=255)
                                                                                                                                       aaaCGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCt                >  1:1003642/1‑150 (MQ=255)
                                                                                                                                         ttgACCCCGTATATTACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCa              >  1:4153903/3‑150 (MQ=255)
                                                                                                                                         ttgACCCCGTATATTACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCa              >  1:3577801/3‑150 (MQ=255)
                                                                                                                                         ttgACCCCGTATATTACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTGCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCa              >  1:1675457/3‑150 (MQ=255)
                                                                                                                                               ccGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTaaa        <  2:1027332/150‑1 (MQ=255)
                                                                                                                                               ccGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTaaa        >  1:1637254/1‑150 (MQ=255)
                                                                                                                                                cGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTaaaa       >  2:1939698/1‑150 (MQ=255)
                                                                                                                                                    atatACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTAAAATCGc   <  1:2307139/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ACATGTCAGTGTGGTACATGGATATCGATACCACCGCCAATTATAAGCTGGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAATATTCTGAATCAGCCAGTCGGCCATTTGCTTTTCCTCATTTAAAATCGCT  >  NC_000913/1314534‑1314832

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: