Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,932,096 C→G 6.3% intergenic (+37/+19) purT → / ← eda phosphoribosylglycinamide formyltransferase 2/KHG/KDPG aldolase; 2‑dehydro‑3‑deoxy‑phosphogluconate/4‑hydroxy‑2‑ oxoglutarate aldolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,932,0960CG 6.3% 921.0 / 43.1 254intergenic (+37/+19)purT/edaphosphoribosylglycinamide formyltransferase 2/KHG/KDPG aldolase; 2‑dehydro‑3‑deoxy‑phosphogluconate/4‑hydroxy‑2‑ oxoglutarate aldolase
Reads supporting (aligned to +/- strand):  ref base C (129/108);  new base G (5/11);  total (134/120)
Fisher's exact test for biased strand distribution p-value = 1.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.25e-01

Only 100 of 257 total aligned reads displayed.

CGGAAATTGATGGCAGCCGTCGTCTGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAACCACCGATGCACAGCACGCTTTTCAGCGCCAGGTAG  >  NC_000913/1931947‑1932244
                                                                                                                                                     |                                                                                                                                                    
   aaaTTGATGGCAGCCGTCGTCTGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGAt                                                                                                                                                   <  1:1863318/150‑1 (MQ=255)
      ttGATGGCAGCCGTCGTCTGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCgg                                                                                                                                                <  1:782843/150‑1 (MQ=255)
       tGATGGCAGCCGTCGTCTGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCGAGGATCggg                                                                                                                                               <  2:2400511/150‑1 (MQ=255)
       tGATGGCAGCCGTCGTCTGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCGAGGATCggg                                                                                                                                               <  2:2449113/150‑1 (MQ=255)
       tGATGGCAGCCGTCGTCTGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCGAGGATCggg                                                                                                                                               <  1:3990835/150‑1 (MQ=255)
        gATGGCAGCCGTCGTCTGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCGAGGATCGGGc                                                                                                                                              <  2:672124/150‑1 (MQ=255)
           ggCAGCCGTCGTCTGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCGAGGATCGGGCAtt                                                                                                                                           <  2:1354908/150‑1 (MQ=255)
             cAGCCGTCGTCTGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCAtttt                                                                                                                                         <  1:1279569/150‑1 (MQ=255)
             cAGCCGTCGTCTGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCAtttt                                                                                                                                         <  1:2315565/150‑1 (MQ=255)
              aGCCGTCGTCTGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGTCGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCGAGGATCGGGCAttttt                                                                                                                                        <  2:3900814/150‑2 (MQ=255)
               gCCGTCGTCTGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGa                                                                                                                                       <  1:3363488/150‑1 (MQ=255)
                ccGTCGTCTGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCCAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCCTTTTGAc                                                                                                                                      >  1:2207209/1‑150 (MQ=255)
                    cgtcTGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACtttt                                                                                                                                  <  2:2944614/150‑1 (MQ=255)
                     gtcTGGGGGTGGCACTGGCTACTGCAGAGATTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACCGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTa                                                                                                                                 <  1:3847653/150‑1 (MQ=255)
                        tGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAg                                                                                                                              >  1:3761386/1‑150 (MQ=255)
                               ggCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAgcg                                                                                                                       >  1:528430/1‑150 (MQ=255)
                                gCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACTGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAgcgc                                                                                                                      <  1:1988368/150‑1 (MQ=255)
                                gCACGGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAgcgc                                                                                                                      <  1:3180098/150‑1 (MQ=255)
                                 cACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGcc                                                                                                                     >  1:1405139/1‑150 (MQ=255)
                                  aCTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCt                                                                                                                    <  1:1442746/150‑1 (MQ=255)
                                   cTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCtt                                                                                                                   <  2:813786/150‑1 (MQ=255)
                                      gCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTa                                                                                                                >  1:464367/1‑150 (MQ=255)
                                        tACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACa                                                                                                              <  1:3170092/150‑1 (MQ=255)
                                         aCTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCTAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAg                                                                                                             <  1:2465963/150‑1 (MQ=255)
                                         aCTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAg                                                                                                             <  2:863053/150‑1 (MQ=255)
                                              agagagTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCa                                                                                                        >  1:3845985/1‑150 (MQ=255)
                                                      ttgttgACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAg                                                                                                >  2:2129770/1‑150 (MQ=255)
                                                         ttgACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCtt                                                                                             >  2:3182416/1‑150 (MQ=255)
                                                         ttgACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCtt                                                                                             <  1:1481641/150‑1 (MQ=255)
                                                            aCGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGt                                                                                          >  1:1555818/1‑150 (MQ=255)
                                                             cGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTa                                                                                         <  2:1583175/150‑1 (MQ=255)
                                                              gCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACTAATATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTat                                                                                        >  2:3230712/1‑149 (MQ=255)
                                                              gCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTaa                                                                                        >  2:1970903/1‑150 (MQ=255)
                                                              gCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTaa                                                                                        <  2:419763/150‑1 (MQ=255)
                                                                 aTTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGc                                                                                     >  1:1175825/1‑150 (MQ=255)
                                                                 aTTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGc                                                                                     >  1:4026413/1‑150 (MQ=255)
                                                                    gAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGt                                                                                  <  2:711005/150‑1 (MQ=255)
                                                                     aaCGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTc                                                                                 <  1:2590485/150‑1 (MQ=255)
                                                                      aCGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCg                                                                                >  2:1789645/1‑150 (MQ=255)
                                                                      aCGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCg                                                                                >  2:2683779/1‑150 (MQ=255)
                                                                       cgcgcgAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGt                                                                               >  1:439843/1‑150 (MQ=255)
                                                                        gcgcgAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTa                                                                              >  2:3299172/1‑150 (MQ=255)
                                                                         cgcgAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTaa                                                                             <  2:2537550/150‑1 (MQ=255)
                                                                          gcgAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCTCGCGCCAGCTTAGTAATGCGGTCGTAAt                                                                            >  1:1836900/1‑150 (MQ=255)
                                                                          gcgAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAAt                                                                            >  2:1078635/1‑150 (MQ=255)
                                                                           cgAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGTCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATc                                                                           <  2:3789236/150‑1 (MQ=255)
                                                                             caGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGc                                                                         <  2:1522786/149‑1 (MQ=255)
                                                                             aaGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGc                                                                         <  2:2580925/150‑1 (MQ=255)
                                                                             aaGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGc                                                                         <  1:30503/150‑1 (MQ=255)
                                                                              aGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGcc                                                                        <  1:2961465/150‑1 (MQ=255)
                                                                              aGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTACTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGcc                                                                        >  1:1655366/1‑150 (MQ=255)
                                                                                cACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCg                                                                      >  1:862390/1‑150 (MQ=255)
                                                                                cACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCg                                                                      >  1:992957/1‑150 (MQ=255)
                                                                                    ccgccgGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTc                                                                  >  1:1809356/1‑150 (MQ=255)
                                                                                      gccgGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTTGATCGGGCTTTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCa                                                                <  2:3879511/150‑1 (MQ=255)
                                                                                        cgGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAgc                                                              <  2:1107701/150‑1 (MQ=255)
                                                                                        cgGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAgc                                                              <  2:226701/150‑1 (MQ=255)
                                                                                           aCAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCa                                                           >  2:3721889/1‑150 (MQ=255)
                                                                                           aCAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCa                                                           >  2:3482510/1‑150 (MQ=255)
                                                                                               gTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTg                                                       >  1:3571295/1‑150 (MQ=255)
                                                                                               gTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTg                                                       >  2:2453364/1‑150 (MQ=255)
                                                                                               gTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTg                                                       >  1:269474/1‑150 (MQ=255)
                                                                                               gTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTg                                                       >  2:1812738/1‑150 (MQ=255)
                                                                                               gTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTg                                                       >  1:3450562/1‑150 (MQ=255)
                                                                                               gTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTg                                                       >  1:3149334/1‑150 (MQ=255)
                                                                                               gTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTg                                                       >  1:3197384/1‑150 (MQ=255)
                                                                                               gTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTGTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCGGCGTAATGCGGGCGTACTCGCCCGCTTCCAGCGCATCTg                                                       >  2:2725784/1‑150 (MQ=255)
                                                                                                 aaaaGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGcc                                                     <  1:410676/150‑1 (MQ=255)
                                                                                                     gTACAGGGATAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTACTGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGaa                                                 <  2:3638087/150‑1 (MQ=255)
                                                                                                      tACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAAc                                                <  1:2658173/150‑1 (MQ=255)
                                                                                                      tACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAAc                                                <  1:485299/150‑1 (MQ=255)
                                                                                                      tACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAAc                                                >  1:2112931/1‑150 (MQ=255)
                                                                                                          gggTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAAccagc                                            <  1:3296328/150‑1 (MQ=255)
                                                                                                           ggTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAAccagcc                                           >  1:3038594/1‑150 (MQ=255)
                                                                                                           ggTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAAccagcc                                           >  2:2548397/1‑150 (MQ=255)
                                                                                                           ggTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGAGCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCGCGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCAGCTGCCGGAAccagcc                                           >  2:830637/1‑150 (MQ=255)
                                                                                                            gTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAAccagcca                                          <  2:3585572/150‑1 (MQ=255)
                                                                                                            gTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAAccagcca                                          >  2:3684398/1‑150 (MQ=255)
                                                                                                            gTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAAccagcca                                          <  2:409674/150‑1 (MQ=255)
                                                                                                             ttAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAAccagccag                                         >  2:3949396/1‑150 (MQ=255)
                                                                                                               aaaCCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGa                                       <  2:3761386/150‑1 (MQ=255)
                                                                                                                aaCCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGaa                                      <  1:3085010/150‑1 (MQ=255)
                                                                                                                 aCCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAac                                     <  1:1048116/150‑1 (MQ=255)
                                                                                                                 aCCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAac                                     <  2:2395061/150‑1 (MQ=255)
                                                                                                                    cGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAaccac                                  <  2:3390139/150‑1 (MQ=255)
                                                                                                                    cGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAaccac                                  >  1:3260326/1‑150 (MQ=255)
                                                                                                                    cGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGGAATCGCCCGCTTCCAGCGCATCTGCCGGCCCCAGCCAGGAaccac                                  >  2:621223/1‑150 (MQ=255)
                                                                                                                     ggCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATGTTGCCTTTTACAGCTTAGCGCCGTCTACAGCTGCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCGGCCAGCGCATCTGCCGGACCCAGCCAGGAcccacc                                 >  2:1256946/1‑150 (MQ=255)
                                                                                                                           aaaaCGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAACCACCGATGca                           <  2:1979774/150‑1 (MQ=255)
                                                                                                                             aaCGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAACCACCGATGcaca                         <  2:2065223/150‑1 (MQ=255)
                                                                                                                                       acaTGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAACCACCGATGCACAGCACGCtttt               <  1:3321282/148‑1 (MQ=255)
                                                                                                                                          tGCCCGATCGAGGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAACCACCGATGCACAGCACGCTTTTCAg            >  2:2832105/1‑150 (MQ=255)
                                                                                                                                             ccGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTACACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAACCACCGATGCACAGCACGCTTTTCAgcgc         <  2:1698845/150‑1 (MQ=255)
                                                                                                                                               gATCCTCGTTCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAACCACCGATGCACAGCACGCTTTTCAGCGCCa       <  2:749324/150‑1 (MQ=255)
                                                                                                                                               gATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAACCACCGATGCACAGCACGCTTTTCAGCGCCa       <  2:3048756/150‑1 (MQ=255)
                                                                                                                                               gATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAACCACCGATGCACAGCACGCTTTTCAGCGCCa       >  1:2754394/1‑150 (MQ=255)
                                                                                                                                                aTCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAACCACCGATGCACAGCACGCTTTTCAGCGCCAg      <  1:2093461/150‑1 (MQ=255)
                                                                                                                                                   cTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGACCCACCGATGCACAGCGCGCTTGTCAGCGCCAGGt  >  1:3653185/1‑149 (MQ=255)
                                                                                                                                                   cTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAACCACCGATGCACAGCACGCTTTTCAGCGCCAGGTa   >  1:1563044/1‑150 (MQ=255)
                                                                                                                                                   cTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAACCACCGATGCACAGCACGCTTTTCAGCGCCAGGTa   <  1:238268/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CGGAAATTGATGGCAGCCGTCGTCTGGGGGTGGCACTGGCTACTGCAGAGAGTGTTGTTGACGCCATTGAACGCGCGAAGCACGCCGCCGGACAGGTAAAAGTACAGGGTTAAACCCGGCAAAAAAACGCTACAAAAATGCCCGATCCTCGATCGGGCATTTTGACTTTTACAGCTTAGCGCCTTCTACAGCTTCACGCGCCAGCTTAGTAATGCGGTCGTAATCGCCCGCTTCCAGCGCATCTGCCGGAACCAGCCAGGAACCACCGATGCACAGCACGCTTTTCAGCGCCAGGTAG  >  NC_000913/1931947‑1932244

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: