BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,639,309	G→C	7.2%	L423V (CTG→GTG)	hisS ←	histidyl tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,639,309	0	G	C	7.2%	788.7 / 3.4	236	L423V (CTG→GTG)	hisS	histidyl tRNA synthetase
Reads supporting (aligned to +/- strand): ref base G (75/138); new base C (19/2); total (96/140)
Fisher's exact test for biased strand distribution p-value = 8.41e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 236 total aligned reads displayed.

GCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACTCACCCAGCACCACAGCAACGCGGGCACCCCATTTATCAGCACGGGC  >  NC_000913/2639166‑2639456

  cagccaATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCATAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTgc                                                                                                                                             <  1:3782236/150‑1 (MQ=255)

      caATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTGGGTCTCGTAAAATTACACCCAGTCCATCTGCTTCCGTAACCCACTAACGTGCGCaa                                                                                                                                         >  1:1249872/1‑150 (MQ=255)

           aGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTGGTGCTCGTAAATTTGCACGCTGTGCATCTCCGTCCGTAACCCAGTAACGTGCGCAAATtcg                                                                                                                                    >  1:2087629/1‑150 (MQ=255)

            gTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATgcgc                                                                                                                                   <  1:374173/150‑1 (MQ=255)

            gTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATgcgc                                                                                                                                   <  2:1523922/150‑1 (MQ=255)

            gTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGGTATCGTAAATTTCCACGCTGTCCTTCTCCGTCCTTAACCCAGTAACGTGCGCAAATgcgc                                                                                                                                   >  1:2456895/1‑150 (MQ=255)

              gcgcCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCgg                                                                                                                                 <  1:3955317/150‑1 (MQ=255)

                 ccAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTGGGTGGGGTAAAATTAAACGCTGGGCGTCTCCATCCAAAAACCAAGAAAGTGCGCAAAAGcgccgcaa                                                                                                                              >  1:3504499/1‑148 (MQ=255)

                 ccAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTa                                                                                                                              <  1:3629014/150‑1 (MQ=255)

                  cAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTAc                                                                                                                             <  1:288550/150‑1 (MQ=255)

                       cccAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTGGGTGTCGGTAATTTACACCCGGTCCGTATCCATCCGGAACCCACAAAAGTGCGCCAAAGCGCGGCGACGCGAt                                                                                                                        >  2:1079776/1‑150 (MQ=255)

                         cAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTAGTTCACGTAAATTTACAAGCTGTACTTATCCTTCCATAACCCAGTAACCTGCGCAAAATCGCGGCTACGCAATcc                                                                                                                      >  2:4115145/1‑150 (MQ=255)

                          aaaaTCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTGGTTCTCGTAAAATTCCACGCGGTCCGTATCCGTACTTAAACCACGAACGTTCGCAAAATCGCGGCTACGCTATACt                                                                                                                     >  2:2866639/1‑150 (MQ=255)

                              tCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTGGTTCGCGTAAAATTACACCCAGGCCGTATCCATCCATAAACCACGAACCTGCGCAAAAGCGCGGCGCACCTATACTgcgc                                                                                                                 >  1:1383372/1‑150 (MQ=255)

                                  cccAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCTCAACt                                                                                                             <  2:1214297/150‑1 (MQ=255)

                                  cccAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTATCGTAAATTTAAAAGCTGTACTTCTCCTTAAATAAAAAATTAAAGTGCGCAAATTCGCGGCTAACCTATACTGCGCaaaa                                                                                                             >  2:659526/1‑148 (MQ=255)

                                  cccAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTGGTTGTCGTAAAATTACACGCTGTGCTTATCCGTCCATAAAACACAAACATGCGCAAAATCGCGGCGACGCGATACAGCGCacaa                                                                                                             >  1:2438081/1‑147 (MQ=255)

                                   ccAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTg                                                                                                            <  1:1441791/150‑1 (MQ=255)

                                   ccAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTGGTTGTCGTAAAATTCAACGCGGTCCGTCACCATCCGTAAACCCGTAACGTGCGCAAATGCGCGGCGACGCGATACGGCGCAAcag                                                                                                            >  1:4103029/1‑148 (MQ=255)

                                    cAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGc                                                                                                           <  1:2597485/150‑1 (MQ=255)

                                    cAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGc                                                                                                           <  1:3251019/150‑1 (MQ=255)

                                     aaCAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTCGTTCTCGTAAATTTACACGCTGTCCTTCTCCTTCCTTAAACCAGTAACGTGCGCAAATGCGCGGCTACGCTAACCGGCGCAACTGcc                                                                                                          >  2:1290298/1‑150 (MQ=255)

                                      aCAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCg                                                                                                         <  1:2513246/150‑1 (MQ=255)

                                         gCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGttt                                                                                                      <  1:1775603/150‑1 (MQ=255)

                                         gCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGttt                                                                                                      <  2:4002120/150‑1 (MQ=255)

                                          ccAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTg                                                                                                     <  2:3963949/150‑1 (MQ=255)

                                          ccAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTg                                                                                                     <  1:917358/150‑1 (MQ=255)

                                           cAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGGTCTCGGAAATTTCCACCCTGGCCGTCTCCGTCCGTAACCCACGAACGGGCCCAAAAGCGCGGCGACCCGATCCAGCGCCACTGCCCGTTGc                                                                                                    >  2:3138731/1‑150 (MQ=255)

                                            aGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGGTCTCGTAAATTTCCACGCTGTCCTTCTCCGTCCTTAACCCAGTAACGTGCGCAAATTCGCGGCTACGCTATCCAGCGCAACTGCCGTGTGCt                                                                                                   >  2:2120932/1‑150 (MQ=255)

                                                 tttGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCa                                                                                              <  1:4127386/150‑1 (MQ=255)

                                                 tttGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCa                                                                                              <  1:15383/150‑1 (MQ=255)

                                                 tttGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCa                                                                                              <  1:342401/150‑1 (MQ=255)

                                                 tttGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGGTGTCGGTAATTTCCACGCGGTCCTTCTCCATCCGTAACCCACTAACGTGCGCAAATTCGCGGCTACGCTATCCTGCGCAACTGCCGTTTTCTAACCa                                                                                              >  2:2290694/1‑150 (MQ=255)

                                                 tttGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTAGTTCTCGTAAATTTACACGCTGTCCTTCTCCATCCATAACCCACTAACCTGCGCAAATGCGCGGCAACGCTATCCAGCGCAACAGCCGTTTGCTCACCa                                                                                              >  2:3820041/1‑150 (MQ=255)

                                                 tttGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTGTTAGTTATCGTAAAATTACACCCGGTCCATCTCCATCCATAACCCACTAAACTTCGCAAATTCCCGCCAACGCTATCCAGCGCAACAGCCCGTAGATCACCa                                                                                              >  2:3684258/1‑150 (MQ=255)

                                                   tGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCaga                                                                                            <  2:1770874/150‑1 (MQ=255)

                                                   tGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCaga                                                                                            <  1:3196016/150‑1 (MQ=255)

                                                   tGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCaga                                                                                            <  1:2745931/150‑1 (MQ=255)

                                                      cATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTGGTTGTCGTAAATTTACACCCAGTCCATATACTTACTTAAAACACTAACGGTCGCAAAATCGCGGCTACGCTAAACAGCGCAACAGCCCTTTGCTCACCAGAgcg                                                                                         >  2:3556468/1‑150 (MQ=255)

                                                           tCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAAt                                                                                    <  1:3070342/150‑1 (MQ=255)

                                                            cAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATc                                                                                   <  1:3673785/150‑1 (MQ=255)

                                                            cAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATc                                                                                   <  2:1327935/150‑1 (MQ=255)

                                                            cAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATc                                                                                   <  1:184817/150‑1 (MQ=255)

                                                            cAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATc                                                                                   <  1:3983662/150‑1 (MQ=255)

                                                               cAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCtt                                                                                <  1:2290694/150‑1 (MQ=255)

                                                               cAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCtt                                                                                <  1:657246/150‑1 (MQ=255)

                                                                aaaaaaGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTc                                                                               <  2:648287/150‑1 (MQ=255)

                                                                aaaaaaGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTc                                                                               <  2:51841/150‑1 (MQ=255)

                                                                        gTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTAGTAAATTTACAAGCTGTCCTTCTCCTTCCATAACCCAGTAACGTGCCCAAAATCGCCGCTACGCTATACAGCGCAACAGCCCGTTTCACAACAGAGCCCAAATCCAACacaacttc                                                                       >  2:3269646/1‑148 (MQ=255)

                                                                         tttAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTActgct                                                                      <  1:3612105/150‑1 (MQ=255)

                                                                         tttAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTACACGCTGTCCGTCTCCTTCCGTAACCCACTAACGTGCGCAAATGCGCCGCTACGCTATCCTGCGCAACTGCCGTTTGCGCACCAGAGCGCAAATCCTTAACAAcagct                                                                      >  2:3429642/1‑150 (MQ=255)

                                                                           tAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCtgt                                                                    <  2:2605887/150‑1 (MQ=255)

                                                                           tAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCtgt                                                                    <  2:2605654/150‑1 (MQ=255)

                                                                           aaaCCGCATCTACCTGCTCGATATCGTTCTCGTAAAATACCACGCTGTCCTTCTCCTTCCTTAACCCACTAAAGTGCGCAAATGCGAGGATAAGCTATCCTGCTCAACTGCCGTTTCATCACCAGAGCTCAAATACTTCACTACTTCtgt                                                                    <  2:2191478/149‑1 (MQ=255)

                                                                               cGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCg                                                                <  2:2371824/150‑1 (MQ=255)

                                                                                  ttCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTa                                                             <  1:3135563/150‑1 (MQ=255)

                                                                                     tACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGcc                                                          <  2:479061/150‑1 (MQ=255)

                                                                                       ccTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCAc                                                        <  2:4058274/150‑1 (MQ=255)

                                                                                         tGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACtt                                                      <  2:752629/150‑1 (MQ=255)

                                                                                         tGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACtt                                                      <  2:2675928/150‑1 (MQ=255)

                                                                                             cGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGa                                                  <  1:607383/150‑1 (MQ=255)

                                                                                             cGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGa                                                  <  1:607253/150‑1 (MQ=255)

                                                                                                  tCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACTCAc                                             <  1:1080323/150‑1 (MQ=255)

                                                                                                  tCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACTCAc                                             >  1:992590/1‑150 (MQ=255)

                                                                                                   cGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACTCAcc                                            <  2:1912009/150‑1 (MQ=255)

                                                                                                        tcGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACTCACCCAGca                                       <  2:877994/150‑1 (MQ=255)

                                                                                                          gTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACTCACCCAGcacc                                     >  1:2879767/1‑150 (MQ=255)

                                                                                                           tAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACTCACCCAGcacca                                    <  1:2555437/150‑1 (MQ=255)

                                                                                                            aaaTTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACTCACCCAGcaccac                                   >  1:3324202/1‑150 (MQ=255)

                                                                                                            aaaTTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACTCACCCAGcaccac                                   >  1:3828204/1‑150 (MQ=255)

                                                                                                             aaTTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACTCACCCAGCACcaca                                  <  2:1731045/150‑1 (MQ=255)

                                                                                                             aaTTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACTCACCCAGCACcaca                                  <  2:4187612/150‑1 (MQ=255)

                                                                                                             aaTTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACTCACCCAGCACcaca                                  <  2:858356/150‑1 (MQ=255)

                                                                                                             aaTTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACTCACCCAGCACcaca                                  <  2:2018103/150‑1 (MQ=255)

                                                                                                                       cTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACTCACCCAGCACCACAGCAACGCggg                        <  2:603603/150‑1 (MQ=255)

                                                                                                                          tccttctccttcCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACTCACCCAGCACCACAGCAACGCGGGCAc                     <  1:3002077/150‑1 (MQ=255)

                                                                                                                           ccttctccttcCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACTCACCCAGCACCACAGCAACGCGGGCAcc                    <  1:2120932/150‑1 (MQ=255)

                                                                                                                            cttctccttcCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACTCACCCAGCACCACAGCAACGCGGGCAccc                   <  1:1098326/150‑1 (MQ=255)

                                                                                                                            cttctccttcCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACTCACCCAGCACCACAGCAACGCGGGCAccc                   >  1:77227/1‑150 (MQ=255)

                                                                                                                            cttctccttcCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACTCACCCAGCACCACAGCAACGCGGGCAccc                   >  1:963201/1‑150 (MQ=255)

                                                                                                                             ttctccttcCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACTCACCCAGCACCACAGCAACGCGGGCAcccc                  >  1:1909229/1‑150 (MQ=255)

                                                                                                                               ctccttcCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACTCACCCAGCACCACAGCAACGCGGGCACCCCAt                >  2:2995771/1‑150 (MQ=255)

                                                                                                                                tccttcctTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACTCACCCAGCACCACAGCAACGCGGGCACCCCAtt               <  1:1338824/150‑1 (MQ=255)

                                                                                                                                tccttcctTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACTCACCCAGCACCACAGCAACGCGGGCACCCCAtt               >  2:3867742/1‑150 (MQ=255)

                                                                                                                                tccttcctTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACTCACCCAGCACCACAGCAACGCGGGCACCCCAtt               <  2:2425745/150‑1 (MQ=255)

                                                                                                                                  cttccttAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACTCACCCAGCACCACAGCAACGCGGGCACCCCATTTa             >  2:543884/1‑150 (MQ=255)

                                                                                                                                  cttccttAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACTCACCCAGCACCACAGCAACGCGGGCACCCCATTTa             >  1:1215333/1‑150 (MQ=255)

                                                                                                                                    tccttAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACTCACCCAGCACCACAGCAACGCGGGCACCCCATTTATc           >  1:3750236/1‑150 (MQ=255)

                                                                                                                                     ccttAACCCAGTAACGTGCGCAACTGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACTCACCCAGCACCACAGCAACGCGGGCACCCCATTTATCa          <  1:2180346/150‑1 (MQ=255)

                                                                                                                                     ccttAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACTCACCCAGCACCACAGCAACGCGGGCACCCCATTTATCa          >  2:1659474/1‑150 (MQ=255)

                                                                                                                                      cttAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACTCACCCAGCACCACAGCAACGCGGGCACCCCATTTATCAg         <  2:3196029/150‑1 (MQ=255)

                                                                                                                                       ttAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACTCACCCAGCACCACAGCAACGCGGGCACCCCATTTATCAGc        >  1:2683220/1‑150 (MQ=255)

                                                                                                                                       ttAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACTCACCCAGCACCACAGCAACGCGGGCACCCCATTTATCAGc        >  1:105680/1‑150 (MQ=255)

                                                                                                                                       ttAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACTCACCCAGCACCACAGCAACGCGGGCACCCCATTTATCAGc        >  2:1519255/1‑150 (MQ=255)

                                                                                                                                           cccAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACTCACCCAGCACCACAGCAACGCGGGCACCCCATTTATCAGCACgg    <  1:2607802/150‑1 (MQ=255)

                                                                                                                                           cccAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACTCACCCAGCACCACAGCAACGCGGGCACCCCATTTATCAGCACgg    <  2:445966/150‑1 (MQ=255)

                                                                                                                                           cccAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACTCACCCAGCACCACAGCAACGCGGGCACCCCATTTATCAGCACgg    <  1:3695604/150‑1 (MQ=255)

                                                                                                                                           cccAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACTCACCCAGCACCACAGCAACGCGGGCACCCCATTTATCAGCACgg    <  1:557962/150‑1 (MQ=255)

                                                                                                                                           cccAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACTCACCCAGCACCACAGCAACGCGGGCACCCCATTTATCAGCACgg    <  2:675960/150‑1 (MQ=255)

                                                                                                                                             cAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACTCACCCAGCACCACAGCAACGCGGGCACCCCATTTATCAGCACGGGc  >  1:2097067/1‑150 (MQ=255)

GCCAGCCAATCAGTGCGCCAACGCCCAAAATCACCCCAACAGCCAGTGCTTTGCCATTTTCAGCAAAAAAGCGTTTAACCGCTTCTACCTGGTCGTTTTCGTTCTCGTAAATTTCCACGCTGTCCTTCTCCTTCCTTAACCCAGTAACGTGCGCAAATGCGCGGCTACGCTATCCTGCGCAACTGCCGTTTGCTCACCAGAGCGCAAATCCTTCACTACTGCTGTGCCGTTAGCCACTTCAGACTCACCCAGCACCACAGCAACGCGGGCACCCCATTTATCAGCACGGGC  >  NC_000913/2639166‑2639456

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`