Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,807,091 T→C 5.1% intergenic (+17/‑41) proW → / → proX glycine betaine/proline ABC transporter permease/glycine betaine/proline ABC transporter periplasmic binding protein; cold shock protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,807,0910TC 5.1% 945.2 / 26.1 276intergenic (+17/‑41)proW/proXglycine betaine/proline ABC transporter permease/glycine betaine/proline ABC transporter periplasmic binding protein; cold shock protein
Reads supporting (aligned to +/- strand):  ref base T (140/121);  new base C (5/9);  total (146/130)
Fisher's exact test for biased strand distribution p-value = 2.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01

Only 100 of 279 total aligned reads displayed.

CGGGATTGTGATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCA  >  NC_000913/2806942‑2807240
                                                                                                                                                     |                                                                                                                                                     
  ggATTGTGATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGa                                                                                                                                                     <  2:296709/150‑1 (MQ=255)
   gATTGTGATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCAGTCATTAAGTAACTCTGCACTTGCCCGGTGAc                                                                                                                                                    >  1:1626852/1‑150 (MQ=255)
     ttGTGATCCTCGCCATTATACTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTTGTCTGCTGACCCGCCCATTCATTAAGTAACTCTTCACTTGCCCGGTGACGc                                                                                                                                                  <  2:2833106/150‑1 (MQ=255)
      tgtgATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGcc                                                                                                                                                 >  1:573580/1‑150 (MQ=255)
       gtgATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCg                                                                                                                                                <  2:2458562/150‑1 (MQ=255)
       gtgATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCAATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCg                                                                                                                                                <  1:275762/150‑1 (MQ=255)
        tgATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCgg                                                                                                                                               >  2:1926162/1‑150 (MQ=255)
        tgATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCgg                                                                                                                                               <  2:1739706/150‑1 (MQ=255)
         gATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGCGTCACCggg                                                                                                                                              <  1:1157475/150‑1 (MQ=255)
         gATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGCGTCACCggg                                                                                                                                              <  1:1829663/150‑1 (MQ=255)
          aTCCTCGCAATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGc                                                                                                                                             <  1:3121100/150‑1 (MQ=255)
            ccTCGCCATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCCTTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCag                                                                                                                                           >  1:3989900/1‑149 (MQ=255)
            ccTCGCCATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGCGTCACCGGGCaa                                                                                                                                           <  1:2198013/150‑2 (MQ=255)
               cGCCATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCAGTAt                                                                                                                                        >  2:4016483/1‑150 (MQ=255)
                 ccATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCa                                                                                                                                      >  1:3883783/1‑150 (MQ=255)
                  cATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCAc                                                                                                                                     <  2:4182130/150‑1 (MQ=255)
                     tATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCt                                                                                                                                  <  1:1066787/150‑1 (MQ=255)
                      aTCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTg                                                                                                                                 <  2:1933819/150‑1 (MQ=255)
                       tCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGc                                                                                                                                <  2:1538520/150‑1 (MQ=255)
                         cTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCa                                                                                                                              >  1:3760153/1‑150 (MQ=255)
                           cGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCaaa                                                                                                                            <  2:3163662/150‑1 (MQ=255)
                            gATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCaaaa                                                                                                                           >  1:3019421/1‑150 (MQ=255)
                               cGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCaaaaaaa                                                                                                                        <  2:630931/150‑1 (MQ=255)
                               cGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCaaaaaaa                                                                                                                        <  2:2033329/150‑1 (MQ=255)
                                gTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAg                                                                                                                       <  2:3441165/150‑1 (MQ=255)
                                    gACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAAt                                                                                                                   >  1:706636/1‑150 (MQ=255)
                                       gCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCCCTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATGAGCACCCTGCCAAAAAAAGGCATAAc                                                                                                                >  2:1838764/1‑150 (MQ=255)
                                       gCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAAc                                                                                                                >  1:582370/1‑150 (MQ=255)
                                         aGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACaa                                                                                                              <  1:2241450/150‑1 (MQ=255)
                                             cGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCg                                                                                                          >  2:3117255/1‑150 (MQ=255)
                                                   gcgcGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGGCGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAg                                                                                                    >  2:3463022/1‑150 (MQ=255)
                                                          tCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACtt                                                                                             >  2:3609768/1‑150 (MQ=255)
                                                            aCGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACtttt                                                                                           >  2:2232829/1‑150 (MQ=255)
                                                            aCGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACtttt                                                                                           >  1:1067004/1‑150 (MQ=255)
                                                            aCGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACtttt                                                                                           >  1:46174/1‑150 (MQ=255)
                                                            aCGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACtttt                                                                                           >  1:4064860/1‑150 (MQ=255)
                                                             cGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGTGACATAGCGTACttttt                                                                                          >  2:1967038/1‑150 (MQ=255)
                                                                 gTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGa                                                                                      <  2:1784848/150‑1 (MQ=255)
                                                                    gcgGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAg                                                                                   >  2:2756782/1‑150 (MQ=255)
                                                                      ggCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCg                                                                                 <  2:3948149/150‑1 (MQ=255)
                                                                      ggCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCg                                                                                 >  1:3237612/1‑150 (MQ=255)
                                                                       gCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGt                                                                                >  2:3674171/1‑150 (MQ=255)
                                                                            cGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGcc                                                                           >  2:1177346/1‑150 (MQ=255)
                                                                             gTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCa                                                                          >  2:1306259/1‑150 (MQ=255)
                                                                             gTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCa                                                                          >  2:1387017/1‑150 (MQ=255)
                                                                             gTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCa                                                                          >  1:1738872/1‑150 (MQ=255)
                                                                                gCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGc                                                                       <  1:3320546/150‑1 (MQ=255)
                                                                                gCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGc                                                                       <  1:1177346/150‑1 (MQ=255)
                                                                                     tACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTAtc                                                                  >  2:1217363/1‑150 (MQ=255)
                                                                                     tACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTAtc                                                                  >  2:3316851/1‑150 (MQ=255)
                                                                                      acacCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATCGCGTACTTTTTGCGACAGCGTTTGCCACGCTTAtct                                                                 >  2:2476887/1‑150 (MQ=255)
                                                                                         ccacTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTa                                                              <  1:2503326/150‑1 (MQ=255)
                                                                                         ccacTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTa                                                              <  2:51756/150‑1 (MQ=255)
                                                                                          cacTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTac                                                             >  2:3355057/1‑150 (MQ=255)
                                                                                          cacTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTac                                                             <  2:3710300/150‑1 (MQ=255)
                                                                                           acTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTaca                                                            >  1:1758903/1‑150 (MQ=255)
                                                                                                   tGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACttt                                                    >  1:2123386/1‑150 (MQ=255)
                                                                                                    gTTGGTGTGCTGACGCGCGCTTTGTTTTAGTAACTCTGGTCTTGCGCGGTGAGGCCGGGCATTTTCTCGCTGCCAATAAAAGGAATAACTATGCGACATAGCGTACTTTTTGCGACTGGGTTTGCCACGCTTATCTCTACTCAAACtttt                                                   <  2:587889/150‑1 (MQ=255)
                                                                                                    gTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACtttt                                                   >  1:3514783/1‑150 (MQ=255)
                                                                                                    gTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACtttt                                                   <  2:761279/150‑1 (MQ=255)
                                                                                                    gTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACtttt                                                   <  2:761505/150‑1 (MQ=255)
                                                                                                       ggTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTtgct                                                >  1:3829542/1‑150 (MQ=255)
                                                                                                          ctgctgACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGcc                                             >  1:403914/1‑150 (MQ=255)
                                                                                                           tgctgACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACCAACTTTGGCTGCCg                                            >  2:2327266/1‑150 (MQ=255)
                                                                                                           tgctgACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCg                                            >  1:1788069/1‑150 (MQ=255)
                                                                                                           tgctgACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCg                                            >  2:148354/1‑150 (MQ=255)
                                                                                                              tgACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATc                                         >  1:3189041/1‑150 (MQ=255)
                                                                                                              tgACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATc                                         <  1:2995649/150‑1 (MQ=255)
                                                                                                              tgACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATc                                         <  1:966671/150‑1 (MQ=255)
                                                                                                              tgACCCGCCCATTCATTAAGTAACTCGGCACGTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATc                                         <  1:928553/150‑1 (MQ=255)
                                                                                                                    gCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCgg                                   >  2:2591082/1‑150 (MQ=255)
                                                                                                                    gCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGCGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCgg                                   >  2:106737/1‑150 (MQ=255)
                                                                                                                     cccATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCggg                                  <  1:1326273/150‑1 (MQ=255)
                                                                                                                     cccATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCggg                                  <  2:307328/150‑1 (MQ=255)
                                                                                                                        attcattAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCaa                               >  2:2125263/1‑150 (MQ=255)
                                                                                                                        attcattAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCaa                               >  1:1449527/1‑150 (MQ=255)
                                                                                                                        attcattAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCaa                               >  2:1776872/1‑150 (MQ=255)
                                                                                                                           cattAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAgg                            <  2:2543296/150‑1 (MQ=255)
                                                                                                                              tAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAATGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCAt                         <  1:529121/150‑1 (MQ=255)
                                                                                                                              tAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCAt                         >  1:2599000/1‑150 (MQ=255)
                                                                                                                               aaGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCAtt                        >  2:4104808/1‑150 (MQ=255)
                                                                                                                                aGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGTGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTa                       <  1:1967038/150‑1 (MQ=255)
                                                                                                                                aGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTa                       <  1:1583185/150‑1 (MQ=255)
                                                                                                                                aGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTa                       <  2:1678381/150‑1 (MQ=255)
                                                                                                                                aGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTa                       <  1:1525515/150‑1 (MQ=255)
                                                                                                                                aGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTa                       <  2:1917084/150‑1 (MQ=255)
                                                                                                                                aGTAACTCTGCACGTGCCCGGTGACGCCGGGCATTATCACCCCGCCAAAAACAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCGACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTa                       <  2:3026375/150‑1 (MQ=255)
                                                                                                                                      tctGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTa                 >  1:3481745/1‑150 (MQ=255)
                                                                                                                                      tctGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTa                 <  1:1901834/150‑1 (MQ=255)
                                                                                                                                      tctGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTa                 <  1:2010507/150‑1 (MQ=255)
                                                                                                                                      tctGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTa                 <  2:2177471/150‑1 (MQ=255)
                                                                                                                                         gataatGCCCGGCGTCACCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATc              >  1:3627687/6‑150 (MQ=255)
                                                                                                                                         gCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATc              <  1:2357044/150‑1 (MQ=255)
                                                                                                                                            aatGCCCGGCGTCACCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAg           >  2:1577614/3‑150 (MQ=255)
                                                                                                                                              tGCCCGGCGTCACCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGtt         >  2:729917/1‑150 (MQ=255)
                                                                                                                                                  cGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCaga     <  2:3992281/150‑1 (MQ=255)
                                                                                                                                                  cGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCaga     <  2:928107/150‑1 (MQ=255)
                                                                                                                                                    gTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGc   >  1:3344571/1‑150 (MQ=255)
                                                                                                                                                    gTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGc   >  1:3736675/1‑150 (MQ=255)
                                                                                                                                                     tGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCa  >  2:3835481/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CGGGATTGTGATCCTCGCCATTATCCTCGATCGTCTGACGCAGGCCGTTGGGCGCGACTCACGCAGTCGCGGCAACCGTCGCTGGTACACCACTGGCCCTGTTGGTCTGCTGACCCGCCCATTCATTAAGTAACTCTGCACTTGCCCGGTGACGCCGGGCATTATCACCCTGCCAAAAAAAGGAATAACAATGCGACATAGCGTACTTTTTGCGACAGCGTTTGCCACGCTTATCTCTACACAAACTTTTGCTGCCGATCTGCCGGGCAAAGGCATTACTGTTAATCCAGTTCAGAGCA  >  NC_000913/2806942‑2807240

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: