Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,239,609 T→G 14.3% intergenic (+64/‑335) alx → / → sstT putative membrane‑bound redox modulator/sodium:serine/threonine symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,239,6090TG14.3% 418.5 / 48.4 175intergenic (+64/‑335)alx/sstTputative membrane‑bound redox modulator/sodium:serine/threonine symporter
Reads supporting (aligned to +/- strand):  ref base T (99/42);  new base G (11/13);  total (110/65)
Fisher's exact test for biased strand distribution p-value = 3.29e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 178 total aligned reads displayed.

TGGGCGTGGTGTTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTAT  >  NC_000913/3239460‑3239757
                                                                                                                                                      |                                                                                                                                                    
tGGGCGTGGTGTTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGACTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCtt                                                                                                                                                      >  1:3202318/1‑150 (MQ=255)
tGGGCGTGGTGTTTGGCATTCTGGTGATGACGTTTATTATAAACGCCTGGGTGAATTATCGGCAAGATAATCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCACTCTCCCtt                                                                                                                                                      >  1:3202324/1‑150 (MQ=255)
   gCGTGGTGTTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCa                                                                                                                                                   <  1:643968/150‑1 (MQ=255)
    cGTGGTGTTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAg                                                                                                                                                  <  1:578647/150‑1 (MQ=255)
      tggtgTTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAggg                                                                                                                                                >  2:1969550/1‑150 (MQ=255)
      tggtgTTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAggg                                                                                                                                                >  1:4113364/1‑150 (MQ=255)
       ggtgTTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGga                                                                                                                                               <  1:1511569/150‑1 (MQ=255)
       ggtgTTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGga                                                                                                                                               <  1:742485/150‑1 (MQ=255)
       ggtgTTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGga                                                                                                                                               <  1:3602144/150‑1 (MQ=255)
        gtgtTTGGCATTCTGGTGATGACGTTTATTATCATCGCCTGGGTGAATTTTCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTTCCCTGTCATTAACATGAGCAGGGTTTTCTCCCTCTCCCTTCCAGGgag                                                                                                                                              >  2:371624/1‑150 (MQ=255)
          gtTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGagag                                                                                                                                            <  2:1895712/150‑1 (MQ=255)
           tttGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAgg                                                                                                                                           >  2:2113177/1‑150 (MQ=255)
            cgggCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAggg                                                                                                                                          <  2:3436450/148‑1 (MQ=255)
             tGGCTTTCTGGTGTTGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATATGCAGCGGGGTTGATAATTTTTAATCTGCCTAAGCCGTTTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTGGAAGGGAGAggga                                                                                                                                         <  1:830800/150‑2 (MQ=255)
             tGGCATTCTGGTGATGACGTTTATTATCACCGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAGTCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCACCCTCTCCCTGGAAGGGAGAggga                                                                                                                                         <  1:3774242/150‑2 (MQ=255)
             tGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGt                                                                                                                                         >  2:3264862/1‑150 (MQ=255)
               gCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCCGGGAGAGGGTCg                                                                                                                                       <  2:3444837/150‑1 (MQ=255)
                     tGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGa                                                                                                                                 >  2:1476160/1‑150 (MQ=255)
                     tGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGa                                                                                                                                 >  2:73235/1‑150 (MQ=255)
                     tGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGa                                                                                                                                 <  2:1424172/150‑1 (MQ=255)
                       gTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAgg                                                                                                                               <  2:3446552/150‑1 (MQ=255)
                             aCGTTTTTTATAAACGCCTGGTTTAATTATCGTAATTATAAGCAGCGGGTTTTATTATTTTTAATCTGCATAAGCGGTTTACCCTGTAATTAAATTGAGCACGTTTTTCTCCCTCTCCCTTCCTGGTTGTGGGTCGGGGTGAGGGTAAtt                                                                                                                         <  1:1958815/150‑1 (MQ=255)
                             aCGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAAtt                                                                                                                         <  2:2592805/150‑1 (MQ=255)
                               gTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCGTCCAGGGAGAGGGTCGGGGTGAGGGTCAtttt                                                                                                                       >  2:2155944/1‑150 (MQ=255)
                               gTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAACTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAAtttt                                                                                                                       >  2:873051/1‑150 (MQ=255)
                                tttATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAAttttt                                                                                                                      >  2:1266682/1‑150 (MQ=255)
                                 ttattaTCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTc                                                                                                                     >  2:2497640/1‑150 (MQ=255)
                                    ttatCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCa                                                                                                                  >  1:3097954/1‑150 (MQ=255)
                                         aaCGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCgct                                                                                                             >  2:4019331/1‑148 (MQ=255)
                                                 gttGAATTATCGGCATGATACGCTGGGGGGTGTATATTTTTTAATCTGCCTAAGCGTGTTACCCTGTCATTAACATGAGCACCGTTTGCGCCCCCCCCCTTCCCGGGAGTGGGTCGGGGTGTGGTTAATTTTTCGCACCGATGCTGGCCt                                                                                                     <  2:3748242/148‑1 (MQ=255)
                                                    tatttATCGGCATGATAAGCAGGGGGGTGGATAATTTTTATTCTGCCTACGCCGTTTACCCTGACATTAACATGAGCACCGTTTACTCCCTCTCCCTTCCTGGGAGTGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGtt                                                                                                  <  2:2805931/147‑1 (MQ=255)
                                                      aTTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTcc                                                                                                >  1:864250/1‑150 (MQ=255)
                                                            ggCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCAc                                                                                          >  2:909879/1‑150 (MQ=255)
                                                             gCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCAcc                                                                                         >  1:2059250/1‑150 (MQ=255)
                                                             gCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCAcc                                                                                         >  1:446169/1‑150 (MQ=255)
                                                             gCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCAcc                                                                                         >  2:1424267/1‑150 (MQ=255)
                                                              cATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCAccc                                                                                        >  1:3451278/1‑150 (MQ=255)
                                                                 gATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTaa                                                                                     >  2:3102057/1‑150 (MQ=255)
                                                                   tAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAAcc                                                                                   >  1:15138/1‑150 (MQ=255)
                                                                    aaGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAAccc                                                                                  >  1:4168954/1‑150 (MQ=255)
                                                                        tgggggtTGTATATTTTTTAATCTGCATAAGCCGTGTACCCTGTCATTAAAATGAGCACGGTTTTCGCCCTCTCCCTCCCTGGGTGTGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCtctc                                                                              <  1:3836806/147‑1 (MQ=25)
                                                                        agcGGGGTGTATAATTTTTAATCTGCCAAACCCGTGTACCCTGTCATTAACATGAGCACCGTTTACTCCCTCACCCTACCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCtctc                                                                              <  2:1389285/150‑1 (MQ=37)
                                                                        agcGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCtctc                                                                              >  1:2435688/1‑150 (MQ=255)
                                                                         gggggtTGTATAATTTTTAATCTGCCTACGCCGTGTACCCTGTCTTTAACATGCGCACGGTTTTCTCCCACTCCCTCCCCGGTTGTGGGTCGGGTTGTGGTTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTcc                                                                             <  2:697736/148‑1 (MQ=25)
                                                                          cGGGGGGGATAATTTTTAATCGGCCTAAGCCGTGTACCCTCTCATTAACAGGCGCACCGTGTGCGCCCGCTCCCTGCCCGGGAGAGGGTGGGGGTGTGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTccc                                                                            <  2:4116353/150‑1 (MQ=25)
                                                                               tGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAc                                                                       >  2:1380874/1‑150 (MQ=255)
                                                                                  tttttttttATTGTGCCTATGCGTTGTACCCTTTTTTTAATTTGAGCACGGTTTTCTCCCTCCCCGTTCGTGGTTTTGGGTGGGGGTTGGGTTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACggg                                                                    <  2:2868672/146‑1 (MQ=16)
                                                                                  aTAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACggg                                                                    >  1:4197632/1‑150 (MQ=255)
                                                                                   tattttttAATCTGCATACGCGTTTAACCGTGTAATTAACTTTAGACGCTTTTTCCCCCTCTCCCTTCCTGGTTGTGGGTGGGGTTTTGTGAAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACgggg                                                                   <  1:2744394/147‑1 (MQ=12)
                                                                                   tAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACgggg                                                                   >  1:2762269/1‑150 (MQ=255)
                                                                                    aaTTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGc                                                                  >  1:2236416/1‑150 (MQ=255)
                                                                                      tttttAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGa                                                                >  2:2358779/1‑150 (MQ=255)
                                                                                      tttttAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGa                                                                >  2:1583494/1‑150 (MQ=255)
                                                                                       ttttAATCTGCCTAAGCCGTGTCCCCTGTCATTCACCTGAGCACCGTTTTCTCCCTCTCCCTTCCCGGGAGTGGGTCGGGGTGTGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAg                                                               <  2:1699315/150‑1 (MQ=37)
                                                                                       ttttAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTCATTTTTCGCACCGATGCTGGCCTGTTCCCCTCCCCCTAACCCTCACCCCAAACGGGGCgcg                                                               >  1:784098/1‑148 (MQ=255)
                                                                                       ttttAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGGTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCgtg                                                               >  1:153886/1‑148 (MQ=255)
                                                                                       ttttAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAg                                                               >  1:3252694/1‑150 (MQ=255)
                                                                                       ttttAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAg                                                               >  1:2286425/1‑150 (MQ=255)
                                                                                       ttttAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAg                                                               >  1:1178824/1‑150 (MQ=255)
                                                                                       ttttAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCCCCCCAAACGGGGCGAg                                                               >  2:1866251/1‑150 (MQ=255)
                                                                                             tCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACt                                                         >  1:105532/1‑150 (MQ=255)
                                                                                               tGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGATCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGa                                                       >  1:342935/1‑150 (MQ=255)
                                                                                                   tAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGa                                                   >  1:1691463/1‑150 (MQ=255)
                                                                                                         gtgtACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCtt                                             >  1:3906461/1‑150 (MQ=255)
                                                                                                              aCCCTGTCATTAACTTGAGCACCGTTTTCGCCCTCTCCCTTCCTGGGTGAGGGTCGGGGTGTGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCtttttt                                         <  2:269950/150‑1 (MQ=25)
                                                                                                              aCCCTGTCATTAACCTGAGCCCCGTGTCCTCCCGCGCCGGGCCCGGGAGCGGGTGGGGGTGCGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCtttttt                                         <  2:977728/150‑1 (MQ=21)
                                                                                                              aCCCTGTCATTAACATGAGCCCGGTGTTCTCCCCCGCCCTTCCGGGGAGCGGGGGGGGGTGTGGGAAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCtttttt                                         <  2:2627069/150‑1 (MQ=25)
                                                                                                              aCCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCCGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCtttttt                                         <  2:3473044/150‑1 (MQ=255)
                                                                                                                ccTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGCCTGACCGAGTCCTGTTTTGa                                       >  2:248358/1‑150 (MQ=255)
                                                                                                                ccTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCACTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGa                                       >  1:1589035/1‑150 (MQ=255)
                                                                                                                ccTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCGAACCCTCTCCCCAAACGGGGCGCGGGGCCTGACCGAGTCCTGTTTTGa                                       >  2:1066202/1‑150 (MQ=255)
                                                                                                                 cTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGAt                                      >  2:3765327/1‑150 (MQ=255)
                                                                                                                       ttAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGtc                                >  2:1180522/1‑150 (MQ=255)
                                                                                                                       ttAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGtc                                >  2:3031684/1‑150 (MQ=255)
                                                                                                                        tAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGtca                               <  2:3166272/150‑1 (MQ=255)
                                                                                                                          aCATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACCGGGCCAGGGGACTGACCCAGTCCTTTTTTGATGTTGtcata                             >  1:3687516/1‑149 (MQ=255)
                                                                                                                          aCATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTATTCGCACCGAGGCGGGCCGGGTCCCCTCAAACTAACACACTACCCAAACGGGGCGAGGGGACAGACCGAGTACTTGTTTGAttttgggata                             >  1:1845689/1‑145 (MQ=255)
                                                                                                                             tGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGt                          >  2:3361310/1‑150 (MQ=255)
                                                                                                                                 cACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCACACCCAAACCCACTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCAACAGTCTgg                      >  1:601732/1‑150 (MQ=255)
                                                                                                                                 cACCGTTTTCGCCCTCCCCCGTCCCGGGAGTGGGTGGGGGTGCGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTgg                      <  1:1319064/150‑1 (MQ=25)
                                                                                                                                  aCCGTTTTCTCCCTCTCCCTTCCCGGGAGAGGGTCGGGGTGGGGGTAATTTTTCGCCCCGGTGCTGGCCGGGTCCCCTCACCCGGACCCTCTCCCCAAACGGGGCGAGGGGACGGTCCGAGGCGTTGTTTGGGGTGGTCATCAGTCTggt                     >  2:2987973/1‑149 (MQ=255)
                                                                                                                                  aCCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGa                     >  2:3183104/1‑150 (MQ=255)
                                                                                                                                    cGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCGTTTTTTGATGTTGTCATCAGTGTGGAAg                   >  1:2548121/1‑150 (MQ=255)
                                                                                                                                    cGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAg                   >  1:4053801/1‑150 (MQ=255)
                                                                                                                                    cGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCACACCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAg                   >  1:2179695/1‑150 (MQ=255)
                                                                                                                                    cGTTTCCCCCCGCCCCCTGCCCGGGAGTGGGTGGGGGTGGGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAg                   <  1:1266682/150‑1 (MQ=25)
                                                                                                                                     gTTTCCCCCCCCCCCCTACCTGGGTGTGGGTGGGGGTGTGGTAAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGc                  <  1:688046/150‑1 (MQ=25)
                                                                                                                                           tccctctccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGCCGGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACg            >  1:2963998/1‑150 (MQ=255)
                                                                                                                                             cctctccctGGAAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGAGGTTGTCATCAGTCTGGAAGCCGCACGtt          >  2:2087339/1‑150 (MQ=255)
                                                                                                                                             cctctccctGGAAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTGTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGtt          >  2:2087320/1‑150 (MQ=255)
                                                                                                                                             cctctccctGGAAGGGAGAGGGTAGGGGTGAGGGTAATTTTTCGCACCGATGCGGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGtg          >  1:3378907/1‑149 (MQ=37)
                                                                                                                                              ctctccctCCGGGGGTGTGGGGGGGGGTGTGGTTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTg         <  1:2001549/150‑1 (MQ=37)
                                                                                                                                              ctctccctACCCGGGAGAGGGCCGGGGTGAGGGAAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCATCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTg         <  1:4132376/150‑1 (MQ=37)
                                                                                                                                              ccccccctGCCGGGGTGTGGGGGGGGGGGTGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTg         <  2:1238647/146‑1 (MQ=37)
                                                                                                                                               tctccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTgg        >  2:2027666/1‑150 (MQ=255)
                                                                                                                                               tctccctGGAAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTgg        >  2:1628095/1‑150 (MQ=255)
                                                                                                                                               tctccctGGAAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTgg        >  2:1628266/1‑150 (MQ=255)
                                                                                                                                               tctccctGGAAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTgg        >  2:541711/1‑150 (MQ=255)
                                                                                                                                               tctccctGGAAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTgg        >  2:2181792/1‑150 (MQ=255)
                                                                                                                                               tctcccgGCCCGGGAGAGGGTGGGGGTTTGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAACCGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTgg        <  1:979731/150‑1 (MQ=37)
                                                                                                                                                      |                                                                                                                                                    
TGGGCGTGGTGTTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTAT  >  NC_000913/3239460‑3239757

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: