Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 134,173 C→T 5.4% T853T (ACC→ACT acnB → aconitate hydratase 2; aconitase B; 2‑methyl‑cis‑aconitate hydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913134,1730CT 5.4% 396.0 / 7.7 129T853T (ACC→ACTacnBaconitate hydratase 2; aconitase B; 2‑methyl‑cis‑aconitate hydratase
Reads supporting (aligned to +/- strand):  ref base C (57/65);  new base T (4/3);  total (61/68)
Fisher's exact test for biased strand distribution p-value = 7.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.93e-01

Only 100 of 132 total aligned reads displayed.

TCCTGGCTTCTGCGGAACTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTAAGG  >  NC_000913/134025‑134322
                                                                                                                                                    |                                                                                                                                                     
tCCTGGCTTCTGCGGAACTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCg                                                                                                                                                      <  2:1399508/150‑1 (MQ=255)
tCCAGGCTTCTGCGGAACTGGCGGCGGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGGTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCg                                                                                                                                                      <  2:348731/150‑1 (MQ=255)
gccTGGCTTCTGCGGAACTGGCGGCTGTTGCGGCGCGGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCg                                                                                                                                                      <  2:1546884/149‑1 (MQ=255)
  cTGGCTTCTGCGGAACTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCgag                                                                                                                                                    <  2:1797744/150‑1 (MQ=255)
  cTGGCTTCTGCGGAACTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCgag                                                                                                                                                    <  2:1286331/150‑1 (MQ=255)
   tGGCTTCTGCGGAACTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCAGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCgaga                                                                                                                                                   <  1:1383584/150‑1 (MQ=255)
      cTTCTGCGGAACTGGCGGCTTTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAg                                                                                                                                                <  2:1496704/150‑1 (MQ=255)
       tgcTGCGGAACTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCGGTGTACTGAGAAAGc                                                                                                                                               <  2:953664/148‑1 (MQ=255)
         cTGCGGAACTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCg                                                                                                                                             <  2:614894/150‑1 (MQ=255)
         cTGCGGAACTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCg                                                                                                                                             >  1:610983/1‑150 (MQ=255)
          tGCGGAACTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCGGTGTACTGAGAAAGctgg                                                                                                                                            <  1:1644393/150‑4 (MQ=255)
           gCGGAACTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGAt                                                                                                                                           >  1:720152/1‑150 (MQ=255)
           gCGGAACTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCAACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGACGAt                                                                                                                                           >  2:1019971/1‑150 (MQ=255)
            cGGAACTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATg                                                                                                                                          >  1:869718/1‑150 (MQ=255)
            cGGAACTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATg                                                                                                                                          >  2:616035/1‑150 (MQ=255)
            cGGAACTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGAAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTACTCACTACACCGAGAAAGCCGATg                                                                                                                                          >  1:795115/1‑150 (MQ=255)
            cGGAACTGGCGGCTGGTGCGGCGCTGATGGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCGGGTAGATAAAACAGCCGGTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATg                                                                                                                                          <  1:1244628/150‑1 (MQ=255)
             ggAACTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCATGTAGATAAAACAGCCGTTGATACTAACCGTTATCTGAACATCAACCAGCTTTCTCAGTACACCGAGAAAGACGagtg                                                                                                                                         >  2:781481/1‑147 (MQ=255)
               aaCTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACTGAGAAAGCCGATgggg                                                                                                                                       <  1:1512590/150‑1 (MQ=255)
               aaCTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATgggg                                                                                                                                       <  2:1139586/150‑1 (MQ=255)
                aCTGGCGGCTGTTGCGGCGCTGATTGGCATACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGTACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGt                                                                                                                                      <  1:261849/150‑1 (MQ=255)
                  tGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGa                                                                                                                                    <  1:657157/150‑1 (MQ=255)
                     cggcggTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGAttt                                                                                                                                 <  1:1736575/150‑1 (MQ=255)
                        cTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTcc                                                                                                                              <  2:830569/150‑1 (MQ=255)
                               ggcgCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGTCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGc                                                                                                                       <  1:117624/150‑1 (MQ=255)
                                   cTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGtt                                                                                                                   >  2:862676/1‑150 (MQ=255)
                                     gATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTa                                                                                                                 >  1:355687/1‑150 (MQ=255)
                                     gATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACAGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTa                                                                                                                 >  2:1153233/1‑150 (MQ=255)
                                      aTTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTaa                                                                                                                <  1:1549432/150‑1 (MQ=255)
                                      aTTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTaa                                                                                                                <  1:731566/150‑1 (MQ=255)
                                       ttGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTaaa                                                                                                               >  2:655519/1‑150 (MQ=255)
                                         ggCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTaaaaa                                                                                                             >  2:1433117/1‑150 (MQ=255)
                                          gCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAg                                                                                                            <  2:774551/150‑1 (MQ=255)
                                          gCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAg                                                                                                            <  1:838488/150‑1 (MQ=255)
                                          gCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAg                                                                                                            <  1:1656371/150‑1 (MQ=255)
                                            aaaCTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTc                                                                                                          >  1:172171/1‑150 (MQ=255)
                                                 gCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAgcgc                                                                                                     >  1:1526575/1‑150 (MQ=255)
                                                  ccGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCa                                                                                                    <  1:540481/150‑1 (MQ=255)
                                                   cGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCAc                                                                                                   >  2:1505296/1‑150 (MQ=255)
                                                    gACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCAcg                                                                                                  <  2:649123/150‑1 (MQ=255)
                                                      cGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCAcgcg                                                                                                <  1:1011513/150‑1 (MQ=255)
                                                           gtagagTACCAGACATACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTTTCTGATCTTCAACCTGCTTTCTCAGTACACCGAGAATGCCGATGGGGTGATTTTCCAGTCTGCGGTTTAAAATGTCAGCGCACGCGCTgcg                                                                                           <  1:1770460/148‑1 (MQ=255)
                                                           gAAGAGTACCAGACCTACGAGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCGTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTgcg                                                                                           <  1:875543/150‑1 (MQ=255)
                                                             agagTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCAGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCa                                                                                         <  1:1801453/150‑1 (MQ=255)
                                                              tagTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGATAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCGTGCGCTgacttt                                                                                        <  2:1231119/149‑6 (MQ=255)
                                                                 tACCAGACGTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGACGATTGGGTGATTTTCCAGACTGCTGTTTAAAAAGTCAGCGCACGCGCTGCGCATaaa                                                                                     <  2:1767007/150‑1 (MQ=255)
                                                                  aCCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTCCCGTGATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAACGTCAGCGCACGCGCTGCGCATaacc                                                                                    >  1:931647/1‑148 (MQ=255)
                                                                   ccAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTGGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACg                                                                                   <  2:918443/150‑1 (MQ=255)
                                                                          tACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAAt                                                                            >  2:485487/1‑150 (MQ=255)
                                                                           aCGTGGCGCAGGTAGATAAAACAGCCGGTGATACTTACCGTTCTCTGAACTTCTACCAGCTATCTCAGTACCCCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATg                                                                           <  1:1200897/150‑1 (MQ=255)
                                                                               ggCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCg                                                                       <  1:1751893/150‑1 (MQ=255)
                                                                                  gcAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTg                                                                    <  2:132465/150‑1 (MQ=255)
                                                                                  gcAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTGTCTCAGTACACCGAGAAAGCCGATGGGGTGAGTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTg                                                                    <  1:183831/150‑1 (MQ=255)
                                                                                     ggTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAAt                                                                 >  1:723795/1‑150 (MQ=255)
                                                                                     ggTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAAt                                                                 >  1:1309162/1‑150 (MQ=255)
                                                                                       tAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATga                                                               >  1:389123/1‑150 (MQ=255)
                                                                                        aGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGATAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATgag                                                              >  1:1705498/1‑150 (MQ=255)
                                                                                        aGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATgag                                                              >  2:613118/1‑150 (MQ=255)
                                                                                        aGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATgag                                                              <  2:905869/150‑1 (MQ=255)
                                                                                         taTAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATgaga                                                             <  1:1238472/149‑1 (MQ=255)
                                                                                         taTAAAACAGACGTTGATACATACCGATATCTAAACTTCAACCAGCTTTCTAAGTACACCGAGAAAGCCTATGGGGTGAGTTTCCAGACTGCCGTTTAAAAAGTCAGCGCACTCGCTGCGCATAAACGACACAATGCCCTCAGAATgaga                                                             >  2:915112/2‑150 (MQ=255)
                                                                                         gATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAGCGACACAATGCCCGGTGAATgagt                                                             >  1:459079/1‑149 (MQ=255)
                                                                                         gATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATgaga                                                             <  1:616035/150‑1 (MQ=255)
                                                                                          aTAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGAt                                                            <  1:386374/150‑1 (MQ=255)
                                                                                           tAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGAtt                                                           <  2:978804/150‑1 (MQ=255)
                                                                                             aaaCAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTcc                                                         >  2:904097/1‑150 (MQ=255)
                                                                                                  gCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACCCCGAGAACGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGc                                                    >  1:1326518/1‑150 (MQ=255)
                                                                                                  gCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGc                                                    >  2:1405722/1‑150 (MQ=255)
                                                                                                       tGATACATACCGTTATCTGACCAGCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAACAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCAtttt                                               <  2:916893/150‑1 (MQ=255)
                                                                                                          tACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCTGGGCAttttttt                                            >  1:1888132/1‑150 (MQ=255)
                                                                                                              tACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTAttt                                        <  1:655519/150‑1 (MQ=255)
                                                                                                                    tATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGTTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTAAAc                                  <  1:168788/150‑1 (MQ=255)
                                                                                                                    tATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTAAAc                                  >  2:1087991/1‑150 (MQ=255)
                                                                                                                      tCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCa                                <  1:1153233/150‑1 (MQ=255)
                                                                                                                         gAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAATTCTGCTCACGCGCTGAGCATAAACGACACAATGCCCGGTGAATGAGATTCCCTGCCATTTTTTTATTTATAAACCaacc                             >  2:293692/1‑147 (MQ=255)
                                                                                                                           aCTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGcc                           >  1:1451497/1‑150 (MQ=255)
                                                                                                                           aCTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGcc                           >  1:791444/1‑150 (MQ=255)
                                                                                                                               cAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTc                       >  1:145179/1‑150 (MQ=255)
                                                                                                                                aaCCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTcc                      <  1:1433117/150‑1 (MQ=255)
                                                                                                                                    ggCTTTCTCGGTGTACTGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTg                  >  1:1387792/2‑150 (MQ=255)
                                                                                                                                    ggCTTTCTCGGTGTACTGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTACCGGGCAGTTTTTTATTTCCAACACATCGCCGTTCCGATg                  >  2:961024/2‑150 (MQ=255)
                                                                                                                                     gCTTTCTCGGTGTACTGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGt                 >  1:1338308/1‑150 (MQ=255)
                                                                                                                                       tttCTCATTACACCGAGAAAGCCGATGTGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCACGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGttt               <  2:283492/150‑1 (MQ=255)
                                                                                                                                       tttCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGttt               >  1:146405/1‑150 (MQ=255)
                                                                                                                                       cttCTCAGTACACCCATAAACCCGAATGGGGGAATTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCTGTGAATGAGAATCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGttt               <  2:381352/149‑1 (MQ=255)
                                                                                                                                        ttCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGtttt              <  2:1742425/150‑1 (MQ=255)
                                                                                                                                        ttCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGtttt              >  1:1777646/1‑150 (MQ=255)
                                                                                                                                           tcAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTtct           >  1:659622/1‑150 (MQ=255)
                                                                                                                                            cAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTTTTTTtctc          >  2:661633/1‑150 (MQ=255)
                                                                                                                                             cgTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCGGTTTTTCTcc         <  2:170097/149‑1 (MQ=255)
                                                                                                                                             aGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTcc         <  1:1116950/150‑1 (MQ=255)
                                                                                                                                              gTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCg        >  2:1010805/1‑150 (MQ=255)
                                                                                                                                              gTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCg        >  2:1017481/1‑150 (MQ=255)
                                                                                                                                               tACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGGGAATGAGATTCCCGGGCATTTTCTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCgg       <  2:368294/150‑1 (MQ=255)
                                                                                                                                               tACACCCAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTGTAAAAAGTCAGCGCACGTGATGCGCATTAACGACAGAATGACGGGGGTTTGAGATTGCCGGGCATTTTTTTATTTCTCAACCATCGCCGTTCCGCTGTTTTTCTCCgg       <  2:1897781/150‑1 (MQ=255)
                                                                                                                                                 cacCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTa     >  1:293984/1‑150 (MQ=255)
                                                                                                                                                 cacCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTa     >  2:1904573/1‑150 (MQ=255)
                                                                                                                                                 cacCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTa     >  1:87397/1‑150 (MQ=255)
                                                                                                                                                 cacCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTa     >  2:1687025/1‑150 (MQ=255)
                                                                                                                                                   ccGAGAAAGCAGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGAGCACGCGCTGCGCATAAACGACACACTGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTAAg   <  1:338758/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TCCTGGCTTCTGCGGAACTGGCGGCTGTTGCGGCGCTGATTGGCAAACTGCCGACGCCGGAAGAGTACCAGACCTACGTGGCGCAGGTAGATAAAACAGCCGTTGATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATCGCCGTTCCGCTGTTTTTCTCCGGTAAGG  >  NC_000913/134025‑134322

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: