Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,491,904 A→T 5.6% intergenic (+54/+18) ydcA → / ← hokB putative periplasmic protein/toxic polypeptide, small

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,491,9040AT 5.6% 340.4 / 3.4 111intergenic (+54/+18)ydcA/hokBputative periplasmic protein/toxic polypeptide, small
Reads supporting (aligned to +/- strand):  ref base A (56/48);  new base T (5/2);  total (61/50)
Fisher's exact test for biased strand distribution p-value = 4.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 112 total aligned reads displayed.

TTCGAAAGGGGGGATCTCACACTGTACGGCAGGCGGCAAATTTGTCTGTAATGATGGTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGTCAGGAGTGTGAATGTCAGAATCGTAATGCAGATAATGAGCAGACAC  >  NC_000913/1491757‑1492048
                                                                                                                                                   |                                                                                                                                                
 tCGAAAGGGGGGATCTCACACTGTACGGCAGGCGACAAATTTGTCTGTAATGATGGTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAAt                                                                                                                                               >  1:1228559/1‑150 (MQ=255)
    aaaGGGGGGATCTCACACTGTACGGCAGGCGGCAAATTTGTCTGTAATGATGGTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATccc                                                                                                                                            <  2:90637/150‑1 (MQ=255)
     aaGGGGGGATCTCACACTGTACGGCAGGCGGCAAATTTGTCTGTAATGATGGTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGCGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATcccc                                                                                                                                           <  2:1713820/150‑1 (MQ=255)
         ggggATCTCACACTGTACGGCAGGCGGCAAATTTGTCTGTAATGATGGTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCgcgc                                                                                                                                       <  2:644270/150‑1 (MQ=255)
         ggggATCTCACACTGTACGGCAGGCGGCAAATTTGTCTGTAATGATGGTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCgcgc                                                                                                                                       >  1:651246/1‑150 (MQ=255)
           ggATCTCACACTGTACGGCAGGCGGCAAATTTGTCTGTAATGATGGTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGATTTTCCCCgcgcat                                                                                                                                     <  1:1408085/150‑3 (MQ=255)
            gATCTCACACTGTACGGCAGGCGGCAAATTTGTCTGTAATGATGGGTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTg                                                                                                                                    <  2:561646/150‑1 (MQ=255)
              tctcACACTGTACGGCAGGCGGCAAATTTGTCTGTAATGATGGTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGcc                                                                                                                                  <  1:869170/150‑1 (MQ=255)
               ctcACACTGTACGGCAGGCGGCAAATTTGTCTGTAATGATGGTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGccc                                                                                                                                 <  2:857150/150‑1 (MQ=255)
               ctcACACTGTACGGCAGGCGGCAAATTTGTCTGTAATGATGGTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGccc                                                                                                                                 <  2:864699/150‑1 (MQ=255)
                 cacaCTGTACGGCAGGCGGCAAATTTGTCTGTAATGATGGTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCtt                                                                                                                               >  2:1417615/1‑150 (MQ=255)
                  acacTGTACGGCAGGCGGCAAATTTGTCTGTAATGATGGTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTa                                                                                                                              >  1:1230497/1‑150 (MQ=255)
                    acTGTACGGCAGGCGGCAAATTTGTCTGTAATGATGGTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTAcc                                                                                                                            <  2:1012541/150‑1 (MQ=255)
                    acTGTACGGCAGGCGGCAAATTTGTCTGTAATGATGGTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTAcc                                                                                                                            <  2:631832/150‑1 (MQ=255)
                     cTGTACGGCAGGCGGCAAATTTGTCTGTAATGATGGTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCACCGCGCTTGCCCTTACCt                                                                                                                           >  1:701713/1‑150 (MQ=255)
                       gTACGGCAGGCGGCAAATTTGTCTGTAATGATGGTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTgg                                                                                                                         >  2:1087910/1‑150 (MQ=255)
                        tACGGCAGGCGGCAAATTTGTCTGTAATGATGGGTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGa                                                                                                                        <  2:739473/150‑1 (MQ=255)
                           tgCAGGCGGCAAATTTGTCTGTCATGATGGTTCTATTCGTGCATCGACAAAAACATGCACTAACTGAAGTGAAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGt                                                                                                                     <  2:315216/149‑1 (MQ=255)
                                  ggcAAATTTGTCTGTAATGATGGTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGcc                                                                                                              >  1:545689/1‑150 (MQ=255)
                                   gcAAATTTGTCTGTAATGATGGTTTTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCa                                                                                                             >  1:1334498/1‑150 (MQ=255)
                                    cAAATTTGTCTGTAATGATGGTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCAt                                                                                                            <  1:467438/150‑1 (MQ=255)
                                    cAAATTTGTCTGTAATGATGGTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCAt                                                                                                            >  1:1431818/1‑150 (MQ=255)
                                    cAAATTTGTCTGTAATGATGGTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCAt                                                                                                            >  1:263309/1‑150 (MQ=255)
                                          tGTCTGTAATGATGGTTCTATTAGTGCCTCGAAAAAACCATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAgcgc                                                                                                      <  2:1828214/150‑1 (MQ=255)
                                          tGTCTGTAATGATGGTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAgcgc                                                                                                      <  2:725433/150‑1 (MQ=255)
                                              tGTAATGATGGTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCCCTTGCCCTTACCTGGACTTGCAGGCCATGAGCgccgca                                                                                                  >  2:907472/1‑150 (MQ=255)
                                               gTAATGATGGTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCaa                                                                                                 >  1:1012186/1‑150 (MQ=255)
                                                 aaTGATGGTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAAcc                                                                                               <  1:1710748/150‑1 (MQ=255)
                                                   tgatgGTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAATGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCTGGGAAAATCCCCGCGATTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAAcctc                                                                                             >  2:151139/1‑150 (MQ=255)
                                                     atgGTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAAGCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAAcctcct                                                                                           <  1:43164/150‑1 (MQ=255)
                                                       ggTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTa                                                                                         >  2:406164/1‑150 (MQ=255)
                                                           cTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCAc                                                                                     <  1:696787/150‑1 (MQ=255)
                                                            tATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCACCCTCCTTATCAcc                                                                                    >  2:189688/1‑150 (MQ=255)
                                                            tATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCAcc                                                                                    >  2:437842/1‑150 (MQ=255)
                                                               tAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTc                                                                                 >  1:946170/1‑150 (MQ=255)
                                                                aGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTcc                                                                                <  1:1635378/150‑1 (MQ=255)
                                                                aGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTcc                                                                                >  1:1470839/1‑150 (MQ=255)
                                                                aGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTcc                                                                                <  1:768905/150‑1 (MQ=255)
                                                                aGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCACCCTCCTTATCACCGTccc                                                                               >  1:253737/1‑150 (MQ=255)
                                                                  tGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCg                                                                              <  2:42931/150‑1 (MQ=255)
                                                                  tGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCg                                                                              >  1:1432190/1‑150 (MQ=255)
                                                                   gCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCgg                                                                             >  1:629934/1‑150 (MQ=255)
                                                                     aTCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGaa                                                                           >  1:1108878/1‑150 (MQ=255)
                                                                     aTCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTCCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGaa                                                                           >  1:1378133/1‑150 (MQ=255)
                                                                      tCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAAc                                                                          >  2:887515/1‑150 (MQ=255)
                                                                        gAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCCCCGTCCCGGAACCg                                                                        >  2:1328303/1‑150 (MQ=255)
                                                                        gAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCg                                                                        <  2:1334498/150‑1 (MQ=255)
                                                                          aaaaaaCATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGcc                                                                      >  1:459391/1‑149 (MQ=255)
                                                                            aaaaCATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGt                                                                    >  1:991524/1‑150 (MQ=255)
                                                                             aaaCATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCGTATCACCGTCCCGGAACCGCAGtt                                                                   >  1:329460/1‑150 (MQ=255)
                                                                              aaCATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTc                                                                  <  1:562801/150‑1 (MQ=255)
                                                                              aaCATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTc                                                                  >  1:385394/1‑150 (MQ=255)
                                                                              aaCATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATAACCGTCCCGGAACCGCAGTTc                                                                  >  1:832194/1‑150 (MQ=255)
                                                                                      cTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCg                                                          >  2:1041539/1‑150 (MQ=255)
                                                                                        aaCTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGtt                                                        >  1:856830/1‑150 (MQ=255)
                                                                                        aaCTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGtt                                                        >  2:1889982/1‑150 (MQ=255)
                                                                                            gAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTc                                                    <  1:1679583/150‑1 (MQ=255)
                                                                                              aGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCgg                                                  >  2:284253/1‑150 (MQ=255)
                                                                                                  tAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGTCa                                              >  2:1212432/1‑150 (MQ=255)
                                                                                                    aaaGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGTCAgg                                            <  1:1651180/150‑1 (MQ=255)
                                                                                                    aaaGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGTCAgg                                            >  1:1732343/1‑150 (MQ=255)
                                                                                                     aaGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGACCCGCAGTTCGTAGAGCGTTTGTCGGGTCAggg                                           >  1:709345/1‑149 (MQ=255)
                                                                                                      aGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGTCAGGAg                                          <  1:1250930/150‑1 (MQ=255)
                                                                                                       ggggTGCCATGAGAAAATGAATCTGATAGTCAAATGCTCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGACCCGCAGTTCGTAGAGCGTTTGTCGGGTCAGGAgt                                         >  2:312792/1‑150 (MQ=255)
                                                                                                        gggTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGTCAGGAgtg                                        <  2:1846799/150‑1 (MQ=255)
                                                                                                          gTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGCCGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTGTGTCGGGGCAGGAGtgtg                                      >  2:357661/1‑150 (MQ=255)
                                                                                                          gTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGTCAGGAGtgtg                                      <  2:21864/150‑1 (MQ=255)
                                                                                                          gTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGTCAGGAGtgtg                                      <  2:1873169/150‑1 (MQ=255)
                                                                                                             ccATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGTCAGGAGTGTGAAt                                   <  2:1727314/150‑1 (MQ=255)
                                                                                                              cATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGTCAGGAGTGTGAATg                                  <  2:1470839/150‑1 (MQ=255)
                                                                                                                tGAGAAAATGAATCTGCTAGTCAAATGCGCGTGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGTCAGGAGTGTGAATGTc                                >  2:926502/1‑150 (MQ=255)
                                                                                                                 tttaaatTGAATCGGGTTGTAAAAGGCGGGGCGAAACCCCCCGCGCTCGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTCTCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGTCAGGAGTGTGAATGTCa                               <  1:1717923/147‑1 (MQ=255)
                                                                                                                 gagaAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGTCAGGAGTGTGAATGTCa                               >  1:779173/1‑150 (MQ=255)
                                                                                                                   gaAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGTCAGGAGTGTGAATGTCAGa                             >  2:237921/1‑150 (MQ=255)
                                                                                                                        tGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCAGGAACCGAAGTTCGAAGAGCGTATGTCGGGTCAGGCGTGTCAATGGCAGAATCGt                        >  1:290655/1‑150 (MQ=255)
                                                                                                                        agAATCTGCTCGTCAAATGCGCGGGGAACATCCCCGCGCTTGCCCTTACCTGCACGTGCAGGCCATGAGCGCAGCAACCTCCTTAACACCGTCCCGGAACCGCAGTGCGTAGAGCGGTTGGCGGGTCAGGAGTGTGAATGTCAGAATCGt                        <  2:134697/149‑1 (MQ=255)
                                                                                                                            tCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGTCAGGAGTGTGAATGTCAGAATCGTAATg                    <  2:1547925/150‑1 (MQ=255)
                                                                                                                             cTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGTCAGGAGTGTGAATGTCAGAATCGTAATGc                   <  1:1422892/150‑1 (MQ=255)
                                                                                                                             cTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGTCAGGAGTGTGAATGTCAGAATCGTAATGc                   <  1:1555568/150‑1 (MQ=255)
                                                                                                                              tGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCACCCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGTCAGGAGTGTGAATGTCAGAATCGTAATGCa                  >  1:334168/1‑150 (MQ=255)
                                                                                                                                cTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGTCAGGAGTGTGAATGTCAGAATCGTAATGCAGa                <  2:454214/150‑1 (MQ=255)
                                                                                                                                  aGTCAAATGCGCCGGGATAATCCCCGCGCTTGCCCTTACCTGGACGTGCCGGCCATGAGCGCAGCAACCTCCTTATCACCCTACCGGAACCGCAGTACGTAGAGCGTTTGTCGGGTCACGCGTGGGAAGGGCCGCAGCGTCATGCAGCTa              >  2:1025144/1‑150 (MQ=255)
                                                                                                                                   gTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGTCAGGAGTGTGAATGTCAGAATCGTAATGCAGATaa             >  1:1552868/1‑150 (MQ=255)
                                                                                                                                     cAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGTCAGGAg                                          <  2:1883806/119‑1 (MQ=255)
                                                                                                                                     cAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGTCAGGAg                                          >  1:1883806/1‑119 (MQ=255)
                                                                                                                                     cAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGTCAGGAGTGTGAATGTCAGAATCGTAATGCAGATAATg           >  2:1558428/1‑150 (MQ=255)
                                                                                                                                     cAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGTCAGGAGTGTGAATGTCAGAATCGTAATGCAGATAATg           <  1:1765893/150‑1 (MQ=255)
                                                                                                                                     cAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGTCAGGAGTGTGAATGTCAGAATCGTAATGCAGATAATg           >  2:1067262/1‑150 (MQ=255)
                                                                                                                                     cAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGTCAGGAGTGTGAATGTCAGAATCGTAATGCAGATAATg           <  2:1228559/150‑1 (MQ=255)
                                                                                                                                      gcaagcgcGGGGATTTTCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGTCAGGAGTGTGAATGTCAGAATCGTATTGCAGAGAATgg          >  2:287383/5‑149 (MQ=255)
                                                                                                                                      aaaTGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGTCAGGAGTGTGAATGTCAGAATCGTAATGCAGATAATGa          >  2:1508232/1‑150 (MQ=255)
                                                                                                                                      aaaTGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGTCAGGAGTGTGAATGTCAGAATCGGAATGCAGATAATGa          >  1:1312604/1‑150 (MQ=255)
                                                                                                                                       aaTGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGTCAGGAGTGTGAATGTCAGAATCGTAATGCAGATAATGAg         <  2:675658/150‑1 (MQ=255)
                                                                                                                                         agcgcGGGGATTTTCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGTCAGGAGTGTGAATGTCAGAATCGTAATGCAGATAATGAGCa       >  2:890657/2‑150 (MQ=255)
                                                                                                                                          gcgcGGGGATTTTCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGCGCGTTTGTCGGGTCAGGAGTGTGAATGTCAGCATCGTAATGCAGATAATGAGCAg      >  1:1816299/1‑150 (MQ=255)
                                                                                                                                           cgcgGGGAAAATCCCCGCGCTTGCCATTACCTGGACGTGCAGGCCATGAGCGCAGCCGCCTCCTGATCTCCGTCCCGGAACCGCAGTTCGTAGCGCGTTTGTCGGGTCAGGAGTGTGAAGGTCAGAATCGTAATGGAGATAATGAGCAgc     >  1:541071/1‑149 (MQ=255)
                                                                                                                                           cgcgGGGAAAATCCCCGCGCTTCCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGTCAGGAGTGTGAATGTCAGAATCGTAATGCAGATTATGCGCAGa     >  2:763037/1‑150 (MQ=255)
                                                                                                                                             cgGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGTCAGGAGTGTGAATGTCAGAATCGTAATGCAGATAATGAGCAGaca   <  1:1864223/150‑1 (MQ=255)
                                                                                                                                             cgGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGTCAGGAGTGTGAATGTCAGAATCGTAATGCAGATAATGAGCAGaca   >  2:744935/1‑150 (MQ=255)
                                                                                                                                              ggggATAATCCCCGCGCTTGCGCTTACCTGGAAGTGCAGGCCATGAGCGCAGGAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGTGAGGTGTGTTAATGTCAGAATCGTAATGCAGATAATTAGCAGacac  <  2:861047/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
TTCGAAAGGGGGGATCTCACACTGTACGGCAGGCGGCAAATTTGTCTGTAATGATGGTTCTATTAGTGCATCGAAAAAAACATGCACTAACTGAAGTGTAAAAGGGGTGCCATGAGAAAATGAATCTGCTAGTCAAATGCGCGGGGAAAATCCCCGCGCTTGCCCTTACCTGGACGTGCAGGCCATGAGCGCAGCAACCTCCTTATCACCGTCCCGGAACCGCAGTTCGTAGAGCGTTTGTCGGGTCAGGAGTGTGAATGTCAGAATCGTAATGCAGATAATGAGCAGACAC  >  NC_000913/1491757‑1492048

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: