Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,555,781 G→C 5.1% intergenic (‑112/+45) maeA ← / ← sra malate dehydrogenase, decarboxylating, NAD‑requiring; malic enzyme/stationary‑phase‑induced ribosome‑associated protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,555,7810GC 5.1% 375.2 / 5.2 122intergenic (‑112/+45)maeA/sramalate dehydrogenase, decarboxylating, NAD‑requiring; malic enzyme/stationary‑phase‑induced ribosome‑associated protein
Reads supporting (aligned to +/- strand):  ref base G (63/50);  new base C (3/4);  total (66/56)
Fisher's exact test for biased strand distribution p-value = 6.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 131 total aligned reads displayed.

TATAAAGCGAACGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGGTCCAGTCC  >  NC_000913/1555633‑1555930
                                                                                                                                                    |                                                                                                                                                     
tataAAGCGAACGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTgcgc                                                                                                                                                      >  2:420845/1‑150 (MQ=255)
tataAAGCGAACGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTgcgc                                                                                                                                                      <  2:1873873/150‑1 (MQ=255)
  taAAGCGAACGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAg                                                                                                                                                    >  2:781589/1‑150 (MQ=255)
   aaaGCGAACGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAgg                                                                                                                                                   <  2:606620/150‑1 (MQ=255)
   aaaGCGAACGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAgg                                                                                                                                                   >  1:413331/1‑150 (MQ=255)
   aaaGCGAACGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTATTTTTTGAATATCCATCCATGGGGGGCTTTTATCGTCTTTGATTTACCGCCAGGGCGTCGGCCACAAGTATAAAGCAGATAAAAACAAAAACACCAATGCGCAgc                                                                                                                                                   >  1:1211600/1‑149 (MQ=255)
      gCGAACGCTGTTTTTATGTTTTTGGTTCCATGTCACTCACTCATTTTTGAATATCCATCCCTGTGGGGCTTTTATCGTCTTTGATTTACAGACAGGGCGTCGGCCTAAAGTATAAAGAAGATAAAAACAAAAACACCATTGCGCAGGCaa                                                                                                                                                >  1:585163/1‑150 (MQ=255)
        gAACGCTGTTTTTTTGTTTTTGGTTACATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATg                                                                                                                                              >  1:353916/1‑150 (MQ=255)
           cGCTGTTTTTTTGTTTTTGGTTCTATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGgtg                                                                                                                                           <  2:1840363/150‑1 (MQ=255)
           cGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCCTGCGCAATGgtg                                                                                                                                           <  2:1082314/150‑1 (MQ=255)
           cGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCCTGCGCAATGgtg                                                                                                                                           <  2:25934/150‑1 (MQ=255)
           cGCTGTTTTTTTGTGTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGgtg                                                                                                                                           <  1:218193/150‑1 (MQ=255)
           cGCTGTGTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGACTATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGGCGGCCTCAAGTATAAAGCACATAAAAACAAAAACACCATTGCGCAGGCAATGgtg                                                                                                                                           <  1:252490/150‑1 (MQ=255)
             cTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCAATCCCTGGTGTTCTTTTATAGTCTTTGCTTTACAGCCAGGGCGTCGGCCTCAAGTATAACGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGtt                                                                                                                                         >  1:331446/1‑150 (MQ=255)
                 ttttttGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAAt                                                                                                                                     <  1:1241025/150‑1 (MQ=255)
                  tttttgtttttgGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCCTGCGCAATGGTGtttttgt                                                                                                                                    <  1:686370/150‑5 (MQ=255)
                   ttttgtttttgGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCg                                                                                                                                   >  1:28208/1‑150 (MQ=255)
                    tttgtttttgGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGCCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCgg                                                                                                                                  >  2:1691915/1‑149 (MQ=255)
                       gtttttgGTTCCTTGTCACTCACTCTTTTTTGAATATCCATCACTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTTCGCAGGCAATGGTGTTTAATCGTCAt                                                                                                                               >  1:336470/1‑150 (MQ=255)
                       gtttttgGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGAAGATAAAAAAAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCAt                                                                                                                               >  1:298090/1‑150 (MQ=255)
                            tgGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCTTATTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAATCAGATAAAAACAAAAACACCATTGCGCAGGCAATGTTGTTTAATCGTCATTTAgg                                                                                                                          >  1:1169033/1‑150 (MQ=255)
                            tgGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAgg                                                                                                                          >  1:367193/1‑150 (MQ=255)
                             ggTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGAAGGCAATGGTGTTTAATCGTCATtgatga                                                                                                                         >  2:112553/1‑150 (MQ=255)
                              gTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGAc                                                                                                                        <  1:1085520/150‑1 (MQ=255)
                                tCCATTTCACTCACACTTTTTTGAATATCCATCCATGGGGGGCTTTTATCGTCTTTGCTTAACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTg                                                                                                                      <  2:366647/150‑1 (MQ=255)
                                    tGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGTCCTCAAGTATATAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATgg                                                                                                                  >  1:262170/1‑150 (MQ=255)
                                    tGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATgg                                                                                                                  >  2:1487287/1‑150 (MQ=255)
                                    tGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATgg                                                                                                                  >  1:1392083/1‑150 (MQ=255)
                                     gTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGt                                                                                                                 >  2:1568844/1‑150 (MQ=255)
                                       cactcactCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTa                                                                                                               >  1:1902705/1‑150 (MQ=255)
                                        actcactcTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTAt                                                                                                              <  2:353916/150‑1 (MQ=255)
                                           cactcTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGaa                                                                                                           >  1:1854697/1‑150 (MQ=255)
                                            actcTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAAt                                                                                                          >  2:1752599/1‑150 (MQ=255)
                                               ctTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTAc                                                                                                       <  2:318162/150‑1 (MQ=255)
                                                ttttttGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACAATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACt                                                                                                      >  1:1412791/1‑150 (MQ=255)
                                                 tttttGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACtt                                                                                                     >  2:842439/1‑150 (MQ=255)
                                                   tttGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGTCGTCGGCCTCAAGTAAAAATCAGATAAAAACAAAAACACCATTGCGCAGGCAATTCAGTTAAATCGTAATTGAGGACTAATGGTTATGAAtatatttt                                                                                                   >  2:45676/1‑143 (MQ=255)
                                                   tttGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGCATTACtttt                                                                                                   >  1:1265784/1‑150 (MQ=255)
                                                    ttGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTc                                                                                                  <  2:326893/150‑1 (MQ=255)
                                                    ttGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTc                                                                                                  <  2:1800851/150‑1 (MQ=255)
                                                         tatCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCgg                                                                                             >  2:789117/1‑150 (MQ=255)
                                                         tatCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCgg                                                                                             >  1:735997/1‑150 (MQ=255)
                                                         tatCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGAGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTTTGCATTACTTTTCAGCgg                                                                                             >  2:377492/1‑150 (MQ=255)
                                                          atccatccCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGTCTGATGGTTATGAATTACTTTTCAGCggg                                                                                            >  1:1606203/1‑150 (MQ=255)
                                                          atccatccCTGGGGGGCTTTTATCGTCTTTGCTTTACAGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTCTCAGCggg                                                                                            >  1:1607409/1‑150 (MQ=255)
                                                            ccatccCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGc                                                                                          >  2:1827119/1‑150 (MQ=255)
                                                             catccCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCg                                                                                         >  1:1909789/1‑150 (MQ=255)
                                                             catccCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCg                                                                                         >  2:776296/1‑150 (MQ=255)
                                                             catccCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCg                                                                                         >  2:69418/1‑150 (MQ=255)
                                                              atccCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGt                                                                                        >  2:1046866/1‑150 (MQ=255)
                                                                cccTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGttt                                                                                      <  2:188964/150‑1 (MQ=255)
                                                                cccTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCAGTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGttt                                                                                      >  1:1676871/1‑150 (MQ=255)
                                                                    ggggggATTTTATCGTCTTTTCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGATATGAATTACTTTTCAGCGGGGCGTTTTCTg                                                                                  <  1:1149112/150‑1 (MQ=255)
                                                                        ggCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCgg                                                                              <  1:981427/150‑1 (MQ=255)
                                                                           ttttATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTg                                                                           >  2:497404/1‑150 (MQ=255)
                                                                             ttATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTggg                                                                         <  1:763972/150‑1 (MQ=255)
                                                                              tATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGt                                                                        >  1:1906209/1‑150 (MQ=255)
                                                                                tCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTa                                                                      <  2:1647502/150‑1 (MQ=255)
                                                                                tCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTa                                                                      <  1:1230546/150‑1 (MQ=255)
                                                                                 cGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTAt                                                                     >  2:307733/1‑150 (MQ=255)
                                                                                  gTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGGCGGTGGGGTTAtt                                                                    >  2:77989/1‑150 (MQ=255)
                                                                                   tCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTAttt                                                                   >  1:969863/1‑150 (MQ=255)
                                                                                   tCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTAttt                                                                   <  2:1704660/150‑1 (MQ=255)
                                                                                       tGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTtacta                                                               <  1:1336641/150‑1 (MQ=255)
                                                                                         cTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACg                                                             >  1:412212/1‑150 (MQ=255)
                                                                                         cTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGGGTTTAATCGTCATTGAGGACGGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACg                                                             >  2:1616826/1‑150 (MQ=255)
                                                                                          tttACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGc                                                            >  2:1132615/1‑150 (MQ=255)
                                                                                            tACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTg                                                          >  2:1010162/1‑150 (MQ=255)
                                                                                             aCCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTgg                                                         <  1:1846494/150‑1 (MQ=255)
                                                                                               cGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGAt                                                       <  1:1440875/150‑1 (MQ=255)
                                                                                                  cAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTg                                                    <  1:783600/150‑1 (MQ=255)
                                                                                                  cAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTg                                                    <  2:1835128/150‑1 (MQ=255)
                                                                                                     ggCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCa                                                 <  1:781589/150‑1 (MQ=255)
                                                                                                     ggCGTCGGCCGCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCa                                                 <  1:788511/150‑1 (MQ=255)
                                                                                                      gCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCAc                                                >  1:524691/1‑150 (MQ=255)
                                                                                                         tCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCtt                                             >  1:340821/1‑150 (MQ=255)
                                                                                                          cGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCTCAGGCAATGGTGTTAAATCGTCATTGAGGACTGATGGTTATGAATAACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTc                                            <  1:931845/150‑1 (MQ=255)
                                                                                                            gCCTCAAGTATAAAGCAGATAAACACAAAAACCCCATTGCGCAGGGAATGGTGTTTAATCGTCATTGAGTACTGATGGTTATGAATTACTTTTCAGAGGGGCGTTTTCTGCCGGTTGGGTTATTTCCTACGCTGGATTTGTCACCTTCgg                                          <  1:130667/150‑1 (MQ=255)
                                                                                                              cTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTa                                        >  2:1891546/1‑150 (MQ=255)
                                                                                                                 aaGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACt                                     >  2:1311070/1‑150 (MQ=255)
                                                                                                                      taAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTAtttt                                >  2:1318253/1‑150 (MQ=255)
                                                                                                                       aaaGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTg                               <  2:1392083/150‑1 (MQ=255)
                                                                                                                        aaGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTTCTACGCTGGATTTGTCACCTTCGGTAACTATTTTgc                              <  1:758432/150‑1 (MQ=255)
                                                                                                                          gCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTgcgg                            >  2:40888/1‑149 (MQ=255)
                                                                                                                           cAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTAAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCt                           >  1:1172077/1‑150 (MQ=255)
                                                                                                                             taTAAAAACAAAAACACCATTGCGCAGGCAATTGTTTTTAATCGTCATTGTGGACTGATGGTTATGAATTACTTTTCAGCTGGTATTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTATCTATTTTGCGCTgg                         <  2:585163/149‑1 (MQ=255)
                                                                                                                                 aaaacaaaaacaCCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGAAACTATTTTGCGCTGGTTAg                     <  1:1351727/150‑1 (MQ=255)
                                                                                                                                     caaaaacaCCATTGCGCAGGCAATTGTGTTTAATCGTCATTGAGGACTGATGGTAATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCGGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAAt                 <  1:112553/150‑1 (MQ=255)
                                                                                                                                     caaaaacaCCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAAt                 <  1:842439/150‑1 (MQ=255)
                                                                                                                                       aaaacaCCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAAttt               >  1:1010575/1‑150 (MQ=255)
                                                                                                                                       aaaacaCCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGAGTTTTCTGCCGGTTGGGTTATTTACTACGCTGTATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAAttt               >  1:1657448/1‑150 (MQ=255)
                                                                                                                                         aacaCCATTTATCATGCAATGGTGTTTATTCGTCTTTGAGGTCTGATTGTTATTTATTTCTTTTCAGCGTTTCGTTTTCTTACTGTTTGGTTATTTTATACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTTGTTAGAAAttttt             <  1:45676/150‑2 (MQ=255)
                                                                                                                                         aacaCCATTGCCTGCGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTa             >  2:1361747/1‑150 (MQ=255)
                                                                                                                                          acacCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTAt            >  2:252675/1‑150 (MQ=255)
                                                                                                                                               aTTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGgtcc       <  1:1487287/150‑1 (MQ=255)
                                                                                                                                                 tGCGCAGGCAATGGTGTTTAATCGTCATTGGGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGgtccag     >  1:486318/1‑150 (MQ=255)
                                                                                                                                                  gcgcAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTGCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGgtccagt    >  2:824207/1‑150 (MQ=255)
                                                                                                                                                   cgcAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGgtccagtc   >  1:806291/1‑150 (MQ=255)
                                                                                                                                                   cgcAGGCAATGGTGTTTAATCGTCATAGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGgtccagtc   <  2:1596087/150‑1 (MQ=255)
                                                                                                                                                    gcAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGgtccagtcc  <  2:262170/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TATAAAGCGAACGCTGTTTTTTTGTTTTTGGTTCCATGTCACTCACTCTTTTTTGAATATCCATCCCTGGGGGGCTTTTATCGTCTTTGCTTTACCGCCAGGGCGTCGGCCTCAAGTATAAAGCAGATAAAAACAAAAACACCATTGCGCAGGCAATGGTGTTTAATCGTCATTGAGGACTGATGGTTATGAATTACTTTTCAGCGGGGCGTTTTCTGCCGGTTGGGTTATTTACTACGCTGGATTTGTCACCTTCGGTAACTATTTTGCGCTGGTTAGAAATTTTATGGTCCAGTCC  >  NC_000913/1555633‑1555930

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: