Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,560,803 C→T 5.4% D43N (GAC→AAC)  ddpB ← D,D‑dipeptide ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,560,8030CT 5.4% 353.9 / 5.8 110D43N (GAC→AAC) ddpBD,D‑dipeptide ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base C (54/50);  new base T (3/3);  total (57/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.94e-01

Only 100 of 113 total aligned reads displayed.

TTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCATGGTATTGATA  >  NC_000913/1560657‑1560952
                                                                                                                                                  |                                                                                                                                                     
ttCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCAcc                                                                                                                                                    >  2:1836384/1‑150 (MQ=255)
    aGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGcc                                                                                                                                                >  1:1737776/1‑150 (MQ=255)
    aGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGGCACCATGAAACAGGGCGCTGACGTAGCTGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGGTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGcc                                                                                                                                                <  2:117818/150‑1 (MQ=255)
     gCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCa                                                                                                                                               >  1:606257/1‑150 (MQ=255)
     gCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCa                                                                                                                                               <  2:1090617/150‑1 (MQ=255)
      cACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAg                                                                                                                                              <  2:1547563/150‑1 (MQ=255)
          ggACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAAc                                                                                                                                          >  1:1506062/1‑150 (MQ=255)
             cGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACcgc                                                                                                                                       >  1:589651/1‑150 (MQ=255)
             cGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACcgc                                                                                                                                       <  1:756404/150‑1 (MQ=255)
               cccGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATTGCATCGCTGGCGCGGTCACCCGCCAGTAACCgcgc                                                                                                                                     >  1:967158/1‑150 (MQ=255)
               cccGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCgcgc                                                                                                                                     >  1:1583210/1‑150 (MQ=255)
               cccGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCgcgc                                                                                                                                     <  2:847928/150‑1 (MQ=255)
               cccGGAACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGGAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTAGCTGGCGAATATTATACACGAAAGCATCGCTGGCGCGGACACCCGCCACTAACCgcgc                                                                                                                                     >  1:1884966/1‑150 (MQ=255)
                  ggTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTTACTGGCGGGTGACCGCGccag                                                                                                                                  <  2:1021600/150‑3 (MQ=255)
                   gTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGa                                                                                                                                 >  1:644262/1‑150 (MQ=255)
                     aCGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATc                                                                                                                               <  2:1357953/150‑1 (MQ=255)
                      cGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATTTTTTCCACGATAGCATCGCTGGAGCGGTCACCCGCCAGTAACCGCGCCGGATCa                                                                                                                              >  2:48587/1‑150 (MQ=255)
                       gAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTTACTGGCGGGTGACCGCGccagcgatg                                                                                                                             <  2:394680/150‑8 (MQ=255)
                       gAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCAc                                                                                                                             >  2:1295105/1‑150 (MQ=255)
                          tGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAg                                                                                                                          <  2:379282/150‑1 (MQ=255)
                            gATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTTTCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAggg                                                                                                                        <  2:1313927/150‑1 (MQ=255)
                            gATGGTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGGTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAggg                                                                                                                        <  1:1017025/150‑1 (MQ=255)
                              tGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGAt                                                                                                                      <  2:1447484/150‑1 (MQ=255)
                               gTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATc                                                                                                                     >  2:1880996/1‑150 (MQ=255)
                                 tCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAg                                                                                                                   >  2:748596/1‑150 (MQ=255)
                                  cccAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGGCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGa                                                                                                                  <  1:1643475/150‑1 (MQ=255)
                                  cccAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCGGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGCTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGa                                                                                                                  >  2:276935/1‑150 (MQ=255)
                                   ccAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGAt                                                                                                                 >  2:917182/1‑150 (MQ=255)
                                    cAGGTCACCATGAAACAGGTCGCTGACGTAGCGGGAAAACTGTACATACAGTGGCTGGTCCAGTCACAGTTGCTGGCGAATATTTTCCACGATCGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATg                                                                                                                <  1:1161875/150‑1 (MQ=255)
                                      ggTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCg                                                                                                              <  2:639321/150‑1 (MQ=255)
                                       gTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCga                                                                                                             >  2:395478/1‑150 (MQ=255)
                                       gTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCga                                                                                                             <  2:1795077/150‑1 (MQ=255)
                                        tCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCgag                                                                                                            >  1:793221/1‑150 (MQ=255)
                                        tCACCATGAAACAGGTCGCAGACTTAGCGGTAAAACTGTTCATACAGTGGCTCGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGTTAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCgag                                                                                                            <  2:832173/150‑1 (MQ=255)
                                        tCACCATGAAACAGGGCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCgag                                                                                                            <  2:1484045/150‑1 (MQ=255)
                                         cACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCgaga                                                                                                           <  2:637357/150‑1 (MQ=255)
                                          cccATGAACCATGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTTCTGGCGAATATTTTTCACGATAGCATCGCTGGCGCGGTCAGCCGACAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGat                                                                                                          <  1:795328/149‑1 (MQ=255)
                                           ccATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGAGAGata                                                                                                         >  2:1015782/1‑150 (MQ=255)
                                            cATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGataa                                                                                                        >  1:924617/1‑150 (MQ=255)
                                            cATGAAACAGGTCACTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGataa                                                                                                        <  1:1707097/150‑1 (MQ=255)
                                             aTGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGataat                                                                                                       >  1:1125830/1‑150 (MQ=255)
                                              tGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGataata                                                                                                      <  1:455078/150‑1 (MQ=255)
                                              tGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGataata                                                                                                      <  2:91600/150‑1 (MQ=255)
                                               gAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGAtaataa                                                                                                     >  2:1471363/1‑150 (MQ=255)
                                                 aaCAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGCTAATAAAc                                                                                                   >  1:1312665/1‑150 (MQ=255)
                                                  aCAGGTCGCTGACGTAGGGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTGGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCTGATGCGAGATAATAAACg                                                                                                  <  2:231338/150‑1 (MQ=255)
                                                   cAGGCCGCTGACGAAGCGGTAAAACTCTACATACAGTGGCTGGCCCAGTCCCAGTTGCTGGCGAATATTATCCACGATAGCAGCTCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGAGCAGATGCGAGATAATAAACgg                                                                                                 <  1:193904/150‑2 (MQ=255)
                                                       tCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATc                                                                                             <  1:10852/150‑1 (MQ=255)
                                                        cGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCTGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGGAACCGCGCCGGATCACCAGGGATCAGATGCGTGATAATAAACGTAATCa                                                                                            >  1:794886/1‑150 (MQ=255)
                                                                    ggTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGTGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGAcgcccg                                                                                <  1:1162841/150‑1 (MQ=255)
                                                                                catagaGTGGCTGGTCGAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATTATAAACGTAATCACGCAGTCGCCCGCCACCTCGAGCa                                                                    <  2:1309880/150‑1 (MQ=255)
                                                                                catacaGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCa                                                                    >  1:528395/1‑150 (MQ=255)
                                                                                     aGTGGCTGGTCCAGTCCTAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGc                                                               >  1:620356/1‑150 (MQ=255)
                                                                                     aGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCAGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGc                                                               <  1:573040/150‑1 (MQ=255)
                                                                                      gTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGcc                                                              >  1:1586178/1‑150 (MQ=255)
                                                                                          cTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCCCCAGcccccc                                                          >  2:1823292/1‑149 (MQ=255)
                                                                                          cTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCca                                                          >  2:1220170/1‑150 (MQ=255)
                                                                                          cTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCca                                                          <  1:1391144/150‑1 (MQ=255)
                                                                                                cAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCAGCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCg                                                    <  2:726724/150‑1 (MQ=255)
                                                                                                 aGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTATTCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGt                                                   <  2:89459/150‑1 (MQ=255)
                                                                                                 aGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGt                                                   >  1:795545/1‑150 (MQ=255)
                                                                                                   tCCCAGTTGCTGGCGAATATTTTCCACGCTAGCATCGAGGGCGCGGTCACCCGACAGTAACCGCGCAGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCTGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTg                                                 <  2:315473/150‑1 (MQ=255)
                                                                                                   tCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTg                                                 <  2:1281600/150‑1 (MQ=255)
                                                                                                    cccAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTgg                                                <  2:1331979/150‑1 (MQ=255)
                                                                                                    cccAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTgg                                                >  1:1375307/1‑150 (MQ=255)
                                                                                                       aGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGt                                             >  1:586057/1‑150 (MQ=255)
                                                                                                        gTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTa                                            >  1:832181/1‑150 (MQ=255)
                                                                                                            cTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAAt                                        >  1:558274/1‑150 (MQ=255)
                                                                                                              ggCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGCGCACCAGCCCCCAGCAGCGTTGGCGTAAAATAc                                      >  1:1728649/1‑150 (MQ=255)
                                                                                                              ggCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATAc                                      >  1:1178126/1‑150 (MQ=255)
                                                                                                              ggCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATAc                                      <  2:40114/150‑1 (MQ=255)
                                                                                                                cGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTc                                    >  2:632216/1‑150 (MQ=255)
                                                                                                                cGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTc                                    <  2:438317/150‑1 (MQ=255)
                                                                                                                cGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCAACACGAGCACCAGCCCCCAGAAGCGTTGGCGTAAAATACTc                                    >  2:1482586/1‑150 (MQ=255)
                                                                                                                  aaTATTTTCCAAGATAGCATCGCTGGCGCGGTCAACCGCCACTAACCGCGCCGGATCACCAGGGAACAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGACCCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCa                                  >  2:263196/1‑150 (MQ=255)
                                                                                                                     atTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGaa                               <  1:395478/150‑1 (MQ=255)
                                                                                                                      ttttCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGatg                              >  1:1143534/1‑148 (MQ=255)
                                                                                                                        ttCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCCCCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGt                            >  2:529715/1‑150 (MQ=255)
                                                                                                                         tCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTc                           >  1:892169/1‑150 (MQ=255)
                                                                                                                            aCGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACg                        <  1:638541/150‑1 (MQ=255)
                                                                                                                             cGATAGCATCGCTGGCGCGTTCACCCGCCAGTAACCGAGCCGGATCAGCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGt                       <  2:192527/150‑1 (MQ=255)
                                                                                                                                 aGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTAtt                   <  2:727630/150‑1 (MQ=255)
                                                                                                                                 aGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTAtt                   >  2:1516297/1‑150 (MQ=255)
                                                                                                                                  gCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTAttt                  <  1:467015/150‑1 (MQ=255)
                                                                                                                                  gCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTAttt                  <  2:1863767/150‑1 (MQ=255)
                                                                                                                                    aTCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTATTCACGCAGACGCCCGCCACCCCGCGCCCCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGGTATTTAc                >  1:1245839/1‑150 (MQ=255)
                                                                                                                                     tCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACt               <  1:1446896/150‑1 (MQ=255)
                                                                                                                                        cTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCAt            >  2:1698740/1‑150 (MQ=255)
                                                                                                                                        cTGGCGCGGTCACCCGCCAGTAACCGCGCAGGATCACCAGCGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGATCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCAt            <  1:1637573/150‑1 (MQ=255)
                                                                                                                                         cggCGCGGTTACTGGCGGGTGACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCATg           >  1:416671/2‑150 (MQ=255)
                                                                                                                                          ggCGCGGTTACTGGCGGGTGACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGGAAAATACTCCAGAAGGTCACGTTATTTACTCATgg          >  2:502155/1‑150 (MQ=255)
                                                                                                                                          ggCGCGGTCACCCGACAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCCCCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCATgg          >  2:1732904/1‑150 (MQ=255)
                                                                                                                                           gcgcGGTTACTGGCGGGTGACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCATGGt         >  1:1556186/1‑150 (MQ=255)
                                                                                                                                           gcgcGGTCACCCGCCAGTAACCGCGCCGGATCACCCGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCATGGt         <  1:1516297/150‑1 (MQ=255)
                                                                                                                                           gcgcGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCATGGt         <  1:1471363/150‑1 (MQ=255)
                                                                                                                                              cgGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCATGGtatt      >  2:856077/1‑150 (MQ=255)
                                                                                                                                              cgGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCACAAGGTCACGTTATTTACTCATGGtatt      <  2:1583210/150‑1 (MQ=255)
                                                                                                                                                ggcACCCGCCAGTAACGGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCATCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCATGGtattga    <  2:1362342/148‑1 (MQ=255)
                                                                                                                                                ggcACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCATGGtattga    <  1:1431110/148‑1 (MQ=255)
                                                                                                                                                 tCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGATCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCATGGtattgat   <  1:917182/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
TTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCATGGTATTGATA  >  NC_000913/1560657‑1560952

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: