Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,560,806 C→T 5.4% G42S (GGT→AGT)  ddpB ← D,D‑dipeptide ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,560,8060CT 5.4% 344.9 / 6.0 111G42S (GGT→AGT) ddpBD,D‑dipeptide ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base C (54/51);  new base T (3/3);  total (57/54)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.03e-01

Only 100 of 118 total aligned reads displayed.

TTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCATGGTATTGATATTG  >  NC_000913/1560657‑1560955
                                                                                                                                                     |                                                                                                                                                     
ttCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCAcc                                                                                                                                                       >  2:1836384/1‑150 (MQ=255)
ttCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCAAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCAcc                                                                                                                                                       <  1:1549177/150‑1 (MQ=255)
    aGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGcc                                                                                                                                                   >  1:1737776/1‑150 (MQ=255)
    aGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGGCACCATGAAACAGGGCGCTGACGTAGCTGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGGTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGcc                                                                                                                                                   <  2:117818/150‑1 (MQ=255)
     gCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCa                                                                                                                                                  >  1:606257/1‑150 (MQ=255)
     gCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCa                                                                                                                                                  <  2:1090617/150‑1 (MQ=255)
      cACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAg                                                                                                                                                 <  2:1547563/150‑1 (MQ=255)
          ggACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAAc                                                                                                                                             >  1:1506062/1‑150 (MQ=255)
             cGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACcgc                                                                                                                                          >  1:589651/1‑150 (MQ=255)
             cGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACcgc                                                                                                                                          <  1:756404/150‑1 (MQ=255)
               cccGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATTGCATCGCTGGCGCGGTCACCCGCCAGTAACCgcgc                                                                                                                                        >  1:967158/1‑150 (MQ=255)
               cccGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCgcgc                                                                                                                                        <  2:847928/150‑1 (MQ=255)
               cccGGAACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGGAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTAGCTGGCGAATATTATACACGAAAGCATCGCTGGCGCGGACACCCGCCACTAACCgcgc                                                                                                                                        >  1:1884966/1‑150 (MQ=255)
                   gTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGa                                                                                                                                    >  1:644262/1‑150 (MQ=255)
                     aCGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATc                                                                                                                                  <  2:1357953/150‑1 (MQ=255)
                      cGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATTTTTTCCACGATAGCATCGCTGGAGCGGTCACCCGCCAGTAACCGCGCCGGATCa                                                                                                                                 >  2:48587/1‑150 (MQ=255)
                       gAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTTACTGGCGGGTGACCGCGccagcgatg                                                                                                                                <  2:394680/150‑8 (MQ=255)
                       gAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCAc                                                                                                                                >  2:144821/1‑150 (MQ=255)
                       gAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCAc                                                                                                                                >  2:1295105/1‑150 (MQ=255)
                          tGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAg                                                                                                                             <  2:379282/150‑1 (MQ=255)
                            gATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTTTCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAggg                                                                                                                           <  2:1313927/150‑1 (MQ=255)
                            gATGGTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGGTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAggg                                                                                                                           <  1:1017025/150‑1 (MQ=255)
                              tGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGAt                                                                                                                         <  2:1447484/150‑1 (MQ=255)
                               gTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATc                                                                                                                        >  2:1880996/1‑150 (MQ=255)
                                 tCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAg                                                                                                                      >  2:748596/1‑150 (MQ=255)
                                  cccAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGGCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGa                                                                                                                     <  1:1643475/150‑1 (MQ=255)
                                   ccAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGAt                                                                                                                    >  2:917182/1‑150 (MQ=255)
                                    cAGGTCACCATGAAACAGGTCGCTGACGTAGCGGGAAAACTGTACATACAGTGGCTGGTCCAGTCACAGTTGCTGGCGAATATTTTCCACGATCGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATg                                                                                                                   <  1:1161875/150‑1 (MQ=255)
                                      ggTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCCGGGATCAGATGCg                                                                                                                 >  2:244309/1‑150 (MQ=255)
                                      ggTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCg                                                                                                                 <  2:639321/150‑1 (MQ=255)
                                       gTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCga                                                                                                                >  2:395478/1‑150 (MQ=255)
                                       gTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCga                                                                                                                <  2:1795077/150‑1 (MQ=255)
                                        tCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCgag                                                                                                               >  1:793221/1‑150 (MQ=255)
                                        tCACCATGAAACAGGGCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCgag                                                                                                               <  2:1484045/150‑1 (MQ=255)
                                          cccATGAACCATGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTTCTGGCGAATATTTTTCACGATAGCATCGCTGGCGCGGTCAGCCGACAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGat                                                                                                             <  1:795328/149‑1 (MQ=255)
                                           ccATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGAGAGata                                                                                                            >  2:1015782/1‑150 (MQ=255)
                                           ccATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTACACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCCCCAGGGATCAGATGCgagaga                                                                                                            >  2:1773504/1‑148 (MQ=255)
                                            cATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGataa                                                                                                           >  1:924617/1‑150 (MQ=255)
                                            cATGAAACAGGTCACTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGataa                                                                                                           <  1:1707097/150‑1 (MQ=255)
                                             aTGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGataat                                                                                                          >  1:1125830/1‑150 (MQ=255)
                                              tGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGataata                                                                                                         <  1:455078/150‑1 (MQ=255)
                                              tGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGataata                                                                                                         <  2:91600/150‑1 (MQ=255)
                                                 aaCAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGCTAATAAAc                                                                                                      >  1:1312665/1‑150 (MQ=255)
                                                  aCAGGTCGCTGACGTAGGGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTGGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCTGATGCGAGATAATAAACg                                                                                                     <  2:231338/150‑1 (MQ=255)
                                                   cAGGCCGCTGACGAAGCGGTAAAACTCTACATACAGTGGCTGGCCCAGTCCCAGTTGCTGGCGAATATTATCCACGATAGCAGCTCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGAGCAGATGCGAGATAATAAACgg                                                                                                    <  1:193904/150‑2 (MQ=255)
                                                       tCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATc                                                                                                <  1:10852/150‑1 (MQ=255)
                                                        cGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCTGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGGAACCGCGCCGGATCACCAGGGATCAGATGCGTGATAATAAACGTAATCa                                                                                               >  1:794886/1‑150 (MQ=255)
                                                        cGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCa                                                                                               <  2:1245330/150‑1 (MQ=255)
                                                         gCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCAc                                                                                              >  1:536147/1‑150 (MQ=255)
                                                                    ggTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGTGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGAcgcccg                                                                                   <  1:1162841/150‑1 (MQ=255)
                                                                         aaCTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATATTAAACGTATTCACGCAGACGCCCGccacc                                                                              >  1:923908/1‑150 (MQ=255)
                                                                                catacaGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCa                                                                       >  1:528395/1‑150 (MQ=255)
                                                                                     aGTGGCTGGTCCAGTCCTAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGc                                                                  >  1:620356/1‑150 (MQ=255)
                                                                                     aGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCAGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGc                                                                  <  1:573040/150‑1 (MQ=255)
                                                                                      gTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGcc                                                                 >  1:1586178/1‑150 (MQ=255)
                                                                                          cTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCCCCAGcccccc                                                             >  2:1823292/1‑149 (MQ=255)
                                                                                          cTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCca                                                             >  2:1220170/1‑150 (MQ=255)
                                                                                          cTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCca                                                             <  1:1391144/150‑1 (MQ=255)
                                                                                                cAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCAGCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCg                                                       <  2:726724/150‑1 (MQ=255)
                                                                                                 aGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTATTCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGt                                                      <  2:89459/150‑1 (MQ=255)
                                                                                                 aGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGt                                                      >  1:795545/1‑150 (MQ=255)
                                                                                                   tCCCAGTTGCTGGCGAATATTTTCCACGCTAGCATCGAGGGCGCGGTCACCCGACAGTAACCGCGCAGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCTGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTg                                                    <  2:315473/150‑1 (MQ=255)
                                                                                                   tCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTg                                                    <  2:1281600/150‑1 (MQ=255)
                                                                                                    cccAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTgg                                                   <  2:1331979/150‑1 (MQ=255)
                                                                                                    cccAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTgg                                                   >  1:1375307/1‑150 (MQ=255)
                                                                                                        gTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTa                                               >  1:832181/1‑150 (MQ=255)
                                                                                                            cTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAAt                                           >  1:558274/1‑150 (MQ=255)
                                                                                                              ggCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGCGCACCAGCCCCCAGCAGCGTTGGCGTAAAATAc                                         >  1:1728649/1‑150 (MQ=255)
                                                                                                              ggCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATAc                                         <  2:40114/150‑1 (MQ=255)
                                                                                                                cGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTc                                       >  2:632216/1‑150 (MQ=255)
                                                                                                                cGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTc                                       <  2:438317/150‑1 (MQ=255)
                                                                                                                cGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCAACACGAGCACCAGCCCCCAGAAGCGTTGGCGTAAAATACTc                                       >  2:1482586/1‑150 (MQ=255)
                                                                                                                  aaTATTTTCCAAGATAGCATCGCTGGCGCGGTCAACCGCCACTAACCGCGCCGGATCACCAGGGAACAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGACCCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCa                                     >  2:263196/1‑150 (MQ=255)
                                                                                                                     atTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGaa                                  <  1:395478/150‑1 (MQ=255)
                                                                                                                      ttttCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGatg                                 >  1:1143534/1‑148 (MQ=255)
                                                                                                                        ttCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCCCCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGt                               >  2:529715/1‑150 (MQ=255)
                                                                                                                            aCGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACg                           <  1:638541/150‑1 (MQ=255)
                                                                                                                             cGATAGCATCGCTGGCGCGTTCACCCGCCAGTAACCGAGCCGGATCAGCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGt                          <  2:192527/150‑1 (MQ=255)
                                                                                                                                 aGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTAtt                      <  2:727630/150‑1 (MQ=255)
                                                                                                                                 aGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTAtt                      >  2:1516297/1‑150 (MQ=255)
                                                                                                                                  gCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTAttt                     <  1:467015/150‑1 (MQ=255)
                                                                                                                                    aTCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTATTCACGCAGACGCCCGCCACCCCGCGCCCCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGGTATTTAc                   >  1:1245839/1‑150 (MQ=255)
                                                                                                                                        cTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCAt               >  2:1698740/1‑150 (MQ=255)
                                                                                                                                        cTGGCGCGGTCACCCGCCAGTAACCGCGCAGGATCACCAGCGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGATCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCAt               <  1:1637573/150‑1 (MQ=255)
                                                                                                                                         cggCGCGGTTACTGGCGGGTGACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCATg              >  1:416671/2‑150 (MQ=255)
                                                                                                                                          ggCGCGGTTACTGGCGGGTGACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGGAAAATACTCCAGAAGGTCACGTTATTTACTCATgg             >  2:502155/1‑150 (MQ=255)
                                                                                                                                          ggCGCGGTCACCCGACAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCCCCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCATgg             >  2:1732904/1‑150 (MQ=255)
                                                                                                                                           gcgcGGTTACTGGCGGGTGACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCATGGt            >  1:1556186/1‑150 (MQ=255)
                                                                                                                                           gcgcGGTCACCCGCCAGTAACCGCGCCGGATCACCCGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCATGGt            <  1:1516297/150‑1 (MQ=255)
                                                                                                                                           gcgcGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCATGGt            <  1:1471363/150‑1 (MQ=255)
                                                                                                                                             gcgGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCCGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCATGGtat          >  2:738088/1‑150 (MQ=255)
                                                                                                                                              cgGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCATGGtatt         >  2:856077/1‑150 (MQ=255)
                                                                                                                                                ggcACCCGCCAGTAACGGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCATCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCATGGtattga       <  2:1362342/148‑1 (MQ=255)
                                                                                                                                                ggcACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCATGGtattga       <  1:1431110/148‑1 (MQ=255)
                                                                                                                                                 tCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGATCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCATGGtattgat      <  1:917182/150‑1 (MQ=255)
                                                                                                                                                  cACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCCCCACGAGCCCCAGCCCCCAGCAGCGTTGGCGTAAAATCCTCCAGAAGGTCACGTTATTTACTCATGGtattgata     >  1:1273036/1‑150 (MQ=255)
                                                                                                                                                    cccGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGGAAAATACTCCAGAAGGTCACGTTATTTACTCATGGtattgatatt   >  1:276822/1‑150 (MQ=255)
                                                                                                                                                     ccGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCATGGtattgatattg  <  2:606257/150‑1 (MQ=255)
                                                                                                                                                     ccGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCATGGtattgatattg  <  2:1506062/150‑1 (MQ=255)
                                                                                                                                                     ccGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCATGGtattgatattg  <  2:644262/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTCCAGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCATGGTATTGATATTG  >  NC_000913/1560657‑1560955

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: