Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,560,807 C→G 5.4% A41A (GCG→GCC ddpB ← D,D‑dipeptide ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,560,8070CG 5.4% 338.7 / 8.4 111A41A (GCG→GCCddpBD,D‑dipeptide ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base C (54/51);  new base G (3/3);  total (57/54)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.68e-01

Only 100 of 118 total aligned reads displayed.

AGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCATGGTATTGATATTGA  >  NC_000913/1560661‑1560956
                                                                                                                                                  |                                                                                                                                                     
aGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGcc                                                                                                                                                    >  1:1737776/1‑150 (MQ=255)
aGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGGCACCATGAAACAGGGCGCTGACGTAGCTGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGGTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGcc                                                                                                                                                    <  2:117818/150‑1 (MQ=255)
 gCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCa                                                                                                                                                   >  1:606257/1‑150 (MQ=255)
 gCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCa                                                                                                                                                   <  2:1090617/150‑1 (MQ=255)
  cACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAg                                                                                                                                                  <  2:1547563/150‑1 (MQ=255)
      ggACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAAc                                                                                                                                              >  1:1506062/1‑150 (MQ=255)
           cccGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATTGCATCGCTGGCGCGGTCACCCGCCAGTAACCgcgc                                                                                                                                         >  1:967158/1‑150 (MQ=255)
           cccGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCgcgc                                                                                                                                         <  2:847928/150‑1 (MQ=255)
           cccGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCgcgc                                                                                                                                         >  1:1583210/1‑150 (MQ=255)
               gTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGa                                                                                                                                     >  1:644262/1‑150 (MQ=255)
                 aCGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATc                                                                                                                                   <  2:1357953/150‑1 (MQ=255)
                  cGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATTTTTTCCACGATAGCATCGCTGGAGCGGTCACCCGCCAGTAACCGCGCCGGATCa                                                                                                                                  >  2:48587/1‑150 (MQ=255)
                   gAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTTACTGGCGGGTGACCGCGccagcgatg                                                                                                                                 <  2:394680/150‑8 (MQ=255)
                   gAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCAc                                                                                                                                 >  2:1295105/1‑150 (MQ=255)
                   gAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCAc                                                                                                                                 >  2:144821/1‑150 (MQ=255)
                      tGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAg                                                                                                                              <  2:379282/150‑1 (MQ=255)
                        gATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTTTCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAggg                                                                                                                            <  2:1313927/150‑1 (MQ=255)
                        gATGGTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGGTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAggg                                                                                                                            <  1:1017025/150‑1 (MQ=255)
                          tGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGAt                                                                                                                          <  2:1447484/150‑1 (MQ=255)
                           gTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATc                                                                                                                         >  2:1880996/1‑150 (MQ=255)
                             tCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAg                                                                                                                       >  2:748596/1‑150 (MQ=255)
                              cccAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGGCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGa                                                                                                                      <  1:1643475/150‑1 (MQ=255)
                               ccAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGAt                                                                                                                     >  2:917182/1‑150 (MQ=255)
                                cAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATg                                                                                                                    >  1:699042/1‑150 (MQ=255)
                                cAGGTCACCATGAAACAGGTCGCTGACGTAGCGGGAAAACTGTACATACAGTGGCTGGTCCAGTCACAGTTGCTGGCGAATATTTTCCACGATCGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATg                                                                                                                    <  1:1161875/150‑1 (MQ=255)
                                  ggTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCCGGGATCAGATGCg                                                                                                                  >  2:244309/1‑150 (MQ=255)
                                  ggTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCg                                                                                                                  <  2:639321/150‑1 (MQ=255)
                                   gTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCga                                                                                                                 >  2:395478/1‑150 (MQ=255)
                                   gTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCga                                                                                                                 <  2:1795077/150‑1 (MQ=255)
                                    tCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCgag                                                                                                                >  1:793221/1‑150 (MQ=255)
                                    tCACCATGAAACAGGGCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCgag                                                                                                                <  2:1484045/150‑1 (MQ=255)
                                      cccATGAACCATGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTTCTGGCGAATATTTTTCACGATAGCATCGCTGGCGCGGTCAGCCGACAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGat                                                                                                              <  1:795328/149‑1 (MQ=255)
                                       ccATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGAGAGata                                                                                                             >  2:1015782/1‑150 (MQ=255)
                                       ccATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTACACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCCCCAGGGATCAGATGCgagaga                                                                                                             >  2:1773504/1‑148 (MQ=255)
                                        cATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGataa                                                                                                            >  1:924617/1‑150 (MQ=255)
                                        cATGAAACAGGTCACTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGataa                                                                                                            <  1:1707097/150‑1 (MQ=255)
                                         aTGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGataat                                                                                                           >  1:1125830/1‑150 (MQ=255)
                                          tGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGataata                                                                                                          <  2:91600/150‑1 (MQ=255)
                                             aaCAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGCTAATAAAc                                                                                                       >  1:1312665/1‑150 (MQ=255)
                                              aCAGGTCGCTGACGTAGGGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTGGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCTGATGCGAGATAATAAACg                                                                                                      <  2:231338/150‑1 (MQ=255)
                                               cAGGCCGCTGACGAAGCGGTAAAACTCTACATACAGTGGCTGGCCCAGTCCCAGTTGCTGGCGAATATTATCCACGATAGCAGCTCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGAGCAGATGCGAGATAATAAACgg                                                                                                     <  1:193904/150‑2 (MQ=255)
                                                   tCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATc                                                                                                 <  1:10852/150‑1 (MQ=255)
                                                   tCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATc                                                                                                 <  1:18102/150‑1 (MQ=255)
                                                    cGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCTGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGGAACCGCGCCGGATCACCAGGGATCAGATGCGTGATAATAAACGTAATCa                                                                                                >  1:794886/1‑150 (MQ=255)
                                                    cGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCa                                                                                                <  2:1245330/150‑1 (MQ=255)
                                                     gCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCAc                                                                                               >  1:536147/1‑150 (MQ=255)
                                                                ggTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGTGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGAcgcccg                                                                                    <  1:1162841/150‑1 (MQ=255)
                                                                     aaCTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATATTAAACGTATTCACGCAGACGCCCGccacc                                                                               >  1:923908/1‑150 (MQ=255)
                                                                            catacaGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCa                                                                        >  1:528395/1‑150 (MQ=255)
                                                                                 aGTGGCTGGTCCAGTCCTAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGc                                                                   >  1:620356/1‑150 (MQ=255)
                                                                                 aGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCAGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGc                                                                   <  1:573040/150‑1 (MQ=255)
                                                                                      cTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCCCCAGcccccc                                                              >  2:1823292/1‑149 (MQ=255)
                                                                                      cTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCca                                                              <  1:1391144/150‑1 (MQ=255)
                                                                                      cTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCca                                                              >  2:1220170/1‑150 (MQ=255)
                                                                                            cAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCAGCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCg                                                        <  2:726724/150‑1 (MQ=255)
                                                                                             aGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTATTCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGt                                                       <  2:89459/150‑1 (MQ=255)
                                                                                             aGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGt                                                       >  1:795545/1‑150 (MQ=255)
                                                                                               tCCCAGTTGCTGGCGAATATTTTCCACGCTAGCATCGAGGGCGCGGTCACCCGACAGTAACCGCGCAGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCTGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTg                                                     <  2:315473/150‑1 (MQ=255)
                                                                                               tCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTg                                                     <  2:1281600/150‑1 (MQ=255)
                                                                                                cccAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTgg                                                    >  1:1375307/1‑150 (MQ=255)
                                                                                                cccAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTgg                                                    <  2:1331979/150‑1 (MQ=255)
                                                                                                   aGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGt                                                 >  1:586057/1‑150 (MQ=255)
                                                                                                    gTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTa                                                >  1:832181/1‑150 (MQ=255)
                                                                                                        cTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAAt                                            >  1:558274/1‑150 (MQ=255)
                                                                                                          ggCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGCGCACCAGCCCCCAGCAGCGTTGGCGTAAAATAc                                          >  1:1728649/1‑150 (MQ=255)
                                                                                                          ggCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATAc                                          <  2:40114/150‑1 (MQ=255)
                                                                                                            cGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTc                                        >  2:632216/1‑150 (MQ=255)
                                                                                                            cGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTc                                        <  2:438317/150‑1 (MQ=255)
                                                                                                            cGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCAACACGAGCACCAGCCCCCAGAAGCGTTGGCGTAAAATACTc                                        >  2:1482586/1‑150 (MQ=255)
                                                                                                              aaTATTTTCCAAGATAGCATCGCTGGCGCGGTCAACCGCCACTAACCGCGCCGGATCACCAGGGAACAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGACCCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCa                                      >  2:263196/1‑150 (MQ=255)
                                                                                                                 atTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGaa                                   <  1:395478/150‑1 (MQ=255)
                                                                                                                  ttttCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGatg                                  >  1:1143534/1‑148 (MQ=255)
                                                                                                                    ttCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCCCCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGt                                >  2:529715/1‑150 (MQ=255)
                                                                                                                     tCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTc                               >  1:892169/1‑150 (MQ=255)
                                                                                                                        aCGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACg                            <  1:638541/150‑1 (MQ=255)
                                                                                                                         cGATAGCATCGCTGGCGCGTTCACCCGCCAGTAACCGAGCCGGATCAGCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGt                           <  2:192527/150‑1 (MQ=255)
                                                                                                                             aGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTAtt                       >  2:1516297/1‑150 (MQ=255)
                                                                                                                             aGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTAtt                       <  2:727630/150‑1 (MQ=255)
                                                                                                                              gCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTAttt                      <  1:467015/150‑1 (MQ=255)
                                                                                                                                aTCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTATTCACGCAGACGCCCGCCACCCCGCGCCCCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGGTATTTAc                    >  1:1245839/1‑150 (MQ=255)
                                                                                                                                    cTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCAt                >  2:1698740/1‑150 (MQ=255)
                                                                                                                                    cTGGCGCGGTCACCCGCCAGTAACCGCGCAGGATCACCAGCGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGATCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCAt                <  1:1637573/150‑1 (MQ=255)
                                                                                                                                     cggCGCGGTTACTGGCGGGTGACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCATg               >  1:416671/2‑150 (MQ=255)
                                                                                                                                      ggCGCGGTTACTGGCGGGTGACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGGAAAATACTCCAGAAGGTCACGTTATTTACTCATgg              >  2:502155/1‑150 (MQ=255)
                                                                                                                                      ggCGCGGTCACCCGACAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCCCCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCATgg              >  2:1732904/1‑150 (MQ=255)
                                                                                                                                       gcgcGGTTACTGGCGGGTGACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCATGGt             >  1:1556186/1‑150 (MQ=255)
                                                                                                                                       gcgcGGTCACCCGCCAGTAACCGCGCCGGATCACCCGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCATGGt             <  1:1516297/150‑1 (MQ=255)
                                                                                                                                       gcgcGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCATGGt             <  1:1471363/150‑1 (MQ=255)
                                                                                                                                         gcgGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCCGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCATGGtat           >  2:738088/1‑150 (MQ=255)
                                                                                                                                          cgGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCATGGtatt          >  2:856077/1‑150 (MQ=255)
                                                                                                                                            ggcACCCGCCAGTAACGGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCATCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCATGGtattga        <  2:1362342/148‑1 (MQ=255)
                                                                                                                                            ggcACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCATGGtattga        <  1:1431110/148‑1 (MQ=255)
                                                                                                                                              cACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCCCCACGAGCCCCAGCCCCCAGCAGCGTTGGCGTAAAATCCTCCAGAAGGTCACGTTATTTACTCATGGtattgata      >  1:1273036/1‑150 (MQ=255)
                                                                                                                                                cccGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCATGGtattgatatt    >  1:435007/1‑150 (MQ=255)
                                                                                                                                                cccGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGGAAAATACTCCAGAAGGTCACGTTATTTACTCATGGtattgatatt    >  1:276822/1‑150 (MQ=255)
                                                                                                                                                 ccGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCATGGtattgatattg   <  2:644262/150‑1 (MQ=255)
                                                                                                                                                 ccGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCATGGtattgatattg   <  2:606257/150‑1 (MQ=255)
                                                                                                                                                 ccGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCATGGtattgatattg   <  2:1506062/150‑1 (MQ=255)
                                                                                                                                                  cGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCATGGtattgatattga  >  1:977303/1‑150 (MQ=255)
                                                                                                                                                  cGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCATGGtattgatattga  >  2:1653384/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
AGCACCGGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCATGGTATTGATATTGA  >  NC_000913/1560661‑1560956

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: