Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,560,814 A→G 5.1% L39S (TTA→TCA)  ddpB ← D,D‑dipeptide ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,560,8140AG 5.1% 360.5 / 6.7 118L39S (TTA→TCA) ddpBD,D‑dipeptide ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base A (56/56);  new base G (3/3);  total (59/59)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.95e-01

Only 100 of 120 total aligned reads displayed.

GGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCATGGTATTGATATTGAAGACCTG  >  NC_000913/1560667‑1560963
                                                                                                                                                   |                                                                                                                                                     
ggACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAAc                                                                                                                                                     >  1:1506062/1‑150 (MQ=255)
   cGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACcgc                                                                                                                                                  >  1:589651/1‑150 (MQ=255)
   cGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACcgc                                                                                                                                                  <  1:756404/150‑1 (MQ=255)
     cccGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATTGCATCGCTGGCGCGGTCACCCGCCAGTAACCgcgc                                                                                                                                                >  1:967158/1‑150 (MQ=255)
     cccGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTTACTGGCGGGTGACCgcgc                                                                                                                                                <  2:485346/150‑1 (MQ=255)
     cccGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCgcgc                                                                                                                                                <  2:847928/150‑1 (MQ=255)
     cccGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCgcgc                                                                                                                                                >  1:1583210/1‑150 (MQ=255)
        ggTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTTACTGGCGGGTGACCGCGccag                                                                                                                                             <  2:1021600/150‑3 (MQ=255)
         gTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGa                                                                                                                                            >  1:644262/1‑150 (MQ=255)
           aCGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATc                                                                                                                                          <  2:1357953/150‑1 (MQ=255)
            cGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATTTTTTCCACGATAGCATCGCTGGAGCGGTCACCCGCCAGTAACCGCGCCGGATCa                                                                                                                                         >  2:48587/1‑150 (MQ=255)
             gAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTTACTGGCGGGTGACCGCGccagcgatg                                                                                                                                        <  2:394680/150‑8 (MQ=255)
             gAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCAc                                                                                                                                        >  2:144821/1‑150 (MQ=255)
                tGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAg                                                                                                                                     <  2:379282/150‑1 (MQ=255)
                  gATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTTTCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAggg                                                                                                                                   <  2:1313927/150‑1 (MQ=255)
                  gATGGTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGGTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAggg                                                                                                                                   <  1:1017025/150‑1 (MQ=255)
                     gTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATc                                                                                                                                >  2:1880996/1‑150 (MQ=255)
                       tCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAg                                                                                                                              >  2:748596/1‑150 (MQ=255)
                        cccAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGGCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGa                                                                                                                             <  1:1643475/150‑1 (MQ=255)
                        cccAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCGGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGCTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGa                                                                                                                             >  2:276935/1‑150 (MQ=255)
                         ccAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGAt                                                                                                                            >  2:917182/1‑150 (MQ=255)
                          cAGGTCACCATGAAACAGGTCGCTGACGTAGCGGGAAAACTGTACATACAGTGGCTGGTCCAGTCACAGTTGCTGGCGAATATTTTCCACGATCGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATg                                                                                                                           <  1:1161875/150‑1 (MQ=255)
                            ggTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCg                                                                                                                         <  2:639321/150‑1 (MQ=255)
                              tCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCgag                                                                                                                       >  1:793221/1‑150 (MQ=255)
                              tCACCATGAAACAGGGCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCgag                                                                                                                       <  2:1484045/150‑1 (MQ=255)
                               cACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCgaga                                                                                                                      <  2:637357/150‑1 (MQ=255)
                                cccATGAACCATGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTTCTGGCGAATATTTTTCACGATAGCATCGCTGGCGCGGTCAGCCGACAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGat                                                                                                                     <  1:795328/149‑1 (MQ=255)
                                 ccATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGAGAGata                                                                                                                    >  2:1015782/1‑150 (MQ=255)
                                 ccATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTACACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCCCCAGGGATCAGATGCgagaga                                                                                                                    >  2:1773504/1‑148 (MQ=255)
                                  cATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGataa                                                                                                                   >  1:924617/1‑150 (MQ=255)
                                  cATGAAACAGGTCACTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGataa                                                                                                                   <  1:1707097/150‑1 (MQ=255)
                                   aTGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGataat                                                                                                                  >  1:1125830/1‑150 (MQ=255)
                                    tGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGataata                                                                                                                 <  1:455078/150‑1 (MQ=255)
                                    tGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGataata                                                                                                                 <  2:91600/150‑1 (MQ=255)
                                     gAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGAtaataa                                                                                                                >  2:1471363/1‑150 (MQ=255)
                                        aCAGGTCGCTGACGTAGGGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTGGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCTGATGCGAGATAATAAACg                                                                                                             <  2:231338/150‑1 (MQ=255)
                                         cAGGCCGCTGACGAAGCGGTAAAACTCTACATACAGTGGCTGGCCCAGTCCCAGTTGCTGGCGAATATTATCCACGATAGCAGCTCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGAGCAGATGCGAGATAATAAACgg                                                                                                            <  1:193904/150‑2 (MQ=255)
                                             tCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATc                                                                                                        <  1:10852/150‑1 (MQ=255)
                                             tCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATc                                                                                                        <  1:18102/150‑1 (MQ=255)
                                              cGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCTGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGGAACCGCGCCGGATCACCAGGGATCAGATGCGTGATAATAAACGTAATCa                                                                                                       >  1:794886/1‑150 (MQ=255)
                                              cGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCa                                                                                                       <  2:1245330/150‑1 (MQ=255)
                                               gCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCAc                                                                                                      >  1:536147/1‑150 (MQ=255)
                                                               aaCTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATATTAAACGTATTCACGCAGACGCCCGccacc                                                                                      >  1:923908/1‑150 (MQ=255)
                                                                      catagaGTGGCTGGTCGAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATTATAAACGTAATCACGCAGTCGCCCGCCACCTCGAGCa                                                                               <  2:1309880/150‑1 (MQ=255)
                                                                      catacaGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCa                                                                               >  1:528395/1‑150 (MQ=255)
                                                                           aGTGGCTGGTCCAGTCCTAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGc                                                                          >  1:620356/1‑150 (MQ=255)
                                                                                cTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCCCCAGcccccc                                                                     >  2:1823292/1‑149 (MQ=255)
                                                                                cTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCca                                                                     <  1:1391144/150‑1 (MQ=255)
                                                                                cTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCca                                                                     >  2:1220170/1‑150 (MQ=255)
                                                                                      cAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCAGCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCg                                                               <  2:726724/150‑1 (MQ=255)
                                                                                       aGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTATTCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGt                                                              <  2:89459/150‑1 (MQ=255)
                                                                                       aGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGt                                                              >  1:795545/1‑150 (MQ=255)
                                                                                         tCCCAGTTGCTGGCGAATATTTTCCACGCTAGCATCGAGGGCGCGGTCACCCGACAGTAACCGCGCAGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCTGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTg                                                            <  2:315473/150‑1 (MQ=255)
                                                                                         tCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTg                                                            <  2:1281600/150‑1 (MQ=255)
                                                                                          cccAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTgg                                                           <  2:1331979/150‑1 (MQ=255)
                                                                                          cccAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTgg                                                           >  1:1375307/1‑150 (MQ=255)
                                                                                             aGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGt                                                        >  1:586057/1‑150 (MQ=255)
                                                                                                  cTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAAt                                                   >  1:558274/1‑150 (MQ=255)
                                                                                                    ggCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGCGCACCAGCCCCCAGCAGCGTTGGCGTAAAATAc                                                 >  1:1728649/1‑150 (MQ=255)
                                                                                                    ggCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATAc                                                 >  1:1178126/1‑150 (MQ=255)
                                                                                                    ggCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATAc                                                 <  2:40114/150‑1 (MQ=255)
                                                                                                      cGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTc                                               >  2:632216/1‑150 (MQ=255)
                                                                                                      cGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTc                                               <  2:438317/150‑1 (MQ=255)
                                                                                                      cGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCAACACGAGCACCAGCCCCCAGAAGCGTTGGCGTAAAATACTc                                               >  2:1482586/1‑150 (MQ=255)
                                                                                                        aaTATTTTCCAAGATAGCATCGCTGGCGCGGTCAACCGCCACTAACCGCGCCGGATCACCAGGGAACAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGACCCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCa                                             >  2:263196/1‑150 (MQ=255)
                                                                                                           atTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGaa                                          <  1:395478/150‑1 (MQ=255)
                                                                                                            ttttCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGatg                                         >  1:1143534/1‑148 (MQ=255)
                                                                                                               tCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTc                                      >  1:892169/1‑150 (MQ=255)
                                                                                                                  aCGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACg                                   <  1:638541/150‑1 (MQ=255)
                                                                                                                   cGATAGCATCGCTGGCGCGTTCACCCGCCAGTAACCGAGCCGGATCAGCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGt                                  <  2:192527/150‑1 (MQ=255)
                                                                                                                       aGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTAtt                              <  2:727630/150‑1 (MQ=255)
                                                                                                                        gCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTAttt                             <  2:1863767/150‑1 (MQ=255)
                                                                                                                        gCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTAttt                             <  1:467015/150‑1 (MQ=255)
                                                                                                                          aTCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTATTCACGCAGACGCCCGCCACCCCGCGCCCCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGGTATTTAc                           >  1:1245839/1‑150 (MQ=255)
                                                                                                                           tCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACt                          <  1:1446896/150‑1 (MQ=255)
                                                                                                                              cTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCAt                       >  2:1698740/1‑150 (MQ=255)
                                                                                                                              cTGGCGCGGTCACCCGCCAGTAACCGCGCAGGATCACCAGCGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGATCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCAt                       <  1:1637573/150‑1 (MQ=255)
                                                                                                                               cggCGCGGTTACTGGCGGGTGACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCATg                      >  1:416671/2‑150 (MQ=255)
                                                                                                                                ggCGCGGTTACTGGCGGGTGACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGGAAAATACTCCAGAAGGTCACGTTATTTACTCATgg                     >  2:502155/1‑150 (MQ=255)
                                                                                                                                ggCGCGGTCACCCGACAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCCCCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCATgg                     >  2:1732904/1‑150 (MQ=255)
                                                                                                                                 gcgcGGTTACTGGCGGGTGACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCATGGt                    >  1:1556186/1‑150 (MQ=255)
                                                                                                                                 gcgcGGTCACCCGCCAGTAACCGCGCCGGATCACCCGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCATGGt                    <  1:1516297/150‑1 (MQ=255)
                                                                                                                                 gcgcGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCATGGt                    <  1:1471363/150‑1 (MQ=255)
                                                                                                                                   gcgGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCCGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCATGGtat                  >  2:738088/1‑150 (MQ=255)
                                                                                                                                    cgGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCATGGtatt                 >  2:856077/1‑150 (MQ=255)
                                                                                                                                    cgGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCACAAGGTCACGTTATTTACTCATGGtatt                 <  2:1583210/150‑1 (MQ=255)
                                                                                                                                      ggcACCCGCCAGTAACGGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCATCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCATGGtattga               <  2:1362342/148‑1 (MQ=255)
                                                                                                                                      ggcACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCATGGtattga               <  1:1431110/148‑1 (MQ=255)
                                                                                                                                       tCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGATCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCATGGtattgat              <  1:917182/150‑1 (MQ=255)
                                                                                                                                        cACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCCCCACGAGCCCCAGCCCCCAGCAGCGTTGGCGTAAAATCCTCCAGAAGGTCACGTTATTTACTCATGGtattgata             >  1:1273036/1‑150 (MQ=255)
                                                                                                                                          cccGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGGAAAATACTCCAGAAGGTCACGTTATTTACTCATGGtattgatatt           >  1:276822/1‑150 (MQ=255)
                                                                                                                                           ccGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCATGGtattgatattg          <  2:1506062/150‑1 (MQ=255)
                                                                                                                                           ccGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCATGGtattgatattg          <  2:606257/150‑1 (MQ=255)
                                                                                                                                            cGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCATGGtattgatattga         >  2:1653384/1‑150 (MQ=255)
                                                                                                                                             gCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCATGGTATTGATATTGaa        <  2:536147/150‑1 (MQ=255)
                                                                                                                                              ccAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCATGGTATTGATATTGAAg       <  1:422416/150‑1 (MQ=255)
                                                                                                                                                 gTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCATGGTATTGATATTGAAGAcc    <  1:1220170/150‑1 (MQ=255)
                                                                                                                                                  tAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCATGGTATTGATATTGAAGACCt   <  2:793221/150‑1 (MQ=255)
                                                                                                                                                   aaCCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCATGGTATTGATATTGAAGACCTg  <  1:1295105/150‑1 (MQ=255)
                                                                                                                                                   aaCCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCATGGTATTGATATTGAAGACCTg  <  1:144821/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
GGACGCCCGGTACGAATGGATGTTCCCAGGTCACCATGAAACAGGTCGCTGACGTAGCGGTAAAACTGTACATACAGTGGCTGGTCCAGTCCCAGTTGCTGGCGAATATTTTCCACGATAGCATCGCTGGCGCGGTCACCCGCCAGTAACCGCGCCGGATCACCAGGGATCAGATGCGAGATAATAAACGTAATCACGCAGACGCCCGCCACCACGAGCACCAGCCCCCAGCAGCGTTGGCGTAAAATACTCCAGAAGGTCACGTTATTTACTCATGGTATTGATATTGAAGACCTG  >  NC_000913/1560667‑1560963

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: