| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,708,844 | C→G | 19.8% | A642A (GCC→GCG) | rsxC → | SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,708,844 | 0 | C | G | 19.8% | 18.9 / 10.6 | 30 | A642A (GCC→GCG) | rsxC | SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex |
| Reads supporting (aligned to +/- strand): ref base C (13/11); new base G (2/4); total (15/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.51e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.66e-01 | |||||||||||
CCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACG‑GGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTAT > NC_000913/1708705‑1708990 | cccGTGCAAAAGGTCGCAAGCTGGAACAGCAACAGGATAATGCGGAACCAGAACAACAGGTCGATCCTCGCAAAGCCGCCGTAGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaac < 2:1722346/150‑1 (MQ=11) ctaaGCTCGCAATCTGGAACATCATCAGGATACTGCGGAACCAGAACAACAGGTCGATCCTCGCAAAGCTGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCTCGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGt < 2:1304808/148‑1 (MQ=11) aaaTCGCGCAATCTGGAAGAGCAACAGGATAATCCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCTCGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTc < 2:534638/150‑1 (MQ=11) aaaGCGCTCAATCTGGAAGATCAACAGGCTACTGCGGAAGCAGAAGAACAGGTCGATCCTCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTc < 1:580642/150‑1 (MQ=14) cTGGAACACCAACAGGAAAATTCGGACCCAGAACACCAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCTCGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCaaa < 1:1073771/150‑1 (MQ=11) aCAGGCTAATGCGGAACCAGAACAACAGGTCGATCCTCGCAAATCCTCCGTCTAAGCCGCTATTGCCCGTGCCAAAGCTCGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGa < 2:632185/150‑1 (MQ=11) tAATCCGGAAGCAGAACTCCTGGTTGATCCTCGTAAATCTGCCGTCGAAGCCGCTATTGCACGTGCCAAAGCTCGCAAGCTGGAACAGCAACAGGCTAATGCCGACCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGc < 2:1546995/150‑1 (MQ=11) aggAACCAGAAGCAGAGGTTGATCCTCGCAAACCCGCCGTAGAAGCAGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTg < 2:1393146/149‑1 (MQ=14) actttgccGATCCGCGGATATCCGCCGTTGAAGCAGCTATTGACCGGGCCAAATCACGCAAGCTGGAACAGCAACAGGCTAATGCCGATCCAGAACAACAGGCCGATCCTCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAg < 2:205122/143‑1 (MQ=11) aTCCGCGCAAAGCAGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTAGATCCGCGCAAAGCGGACGTCGCACCCCGTATATCCCGACCCAGAGCCCGCaacg > 1:1731380/1‑148 (MQ=14) cgccgTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAACCG‑GGAACAGCAAcc > 1:617773/1‑150 (MQ=255) gccgTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGATCCAGAACAACAGGTAGATCCGCGCAAAGCGGCCGACGAAGCCGCAATTTCCCGAGCCAAAGCACGCAAAAG‑GGAACAGCAAcac > 2:590346/1‑148 (MQ=14) gccgTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTAGTGCGCGAGCCAAAGCGCGCACGCG‑GGAACAGCAACCg > 1:610124/1‑150 (MQ=255) gTCCAAGCCGCTATTGCCCGTCCCAACGCTCGCAAGCTGGAACAGCAACAGGCTAAGGCCGAGCCAGAACACCAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACG‑GGAACAGCAACCGGCt < 1:10915/150‑1 (MQ=17) gCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAAGAACAGGTGGATCCGCGCAAAGCGGCCGTAGAAGCCGCTATTGCCCGCGCCCAAGCCCG‑AAACGCGGACCAGCAGACGGGTTATTCGGAGGCcgaacagcaa > 2:325840/1‑144 (MQ=2) ccAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAAGAACAGGTAGATCCGCGCAAAGCCGCAGTAGACGCCGCTATTGCCCGCGCAAAAGCCAGAAAACA‑TCAGCAGCAACAGGCTAATGGAAAGACTGAATAACAgg > 2:827657/1‑150 (MQ=2) tcgaaaGCTGGATCAGCAACAGGATAATGCCGACCCAGAACCCCTGGTGGATCCTCGCAAATCCGCCGTCGAAGCAGCTATTGCCCGAGCCAAAGCGCGCAAACG‑GGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATcc < 1:1729258/146‑1 (MQ=18) gcGCAAGCTGGAACAGCAACAGGCTAATGCGGATCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACg‑gg < 2:870475/106‑1 (MQ=255) ggAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTAGATCCGCGCAAAGCGGCAGTAGCAGCCGCTATTGCCCGCGCACAAGCCCGCAAAGC‑GGAACAGCAACAGGGTTATGCCGAGCCAGAAGTAGAGGTGGGTCAGCGCAGAGc > 2:62166/1‑150 (MQ=2) gCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCCCGCAAACG‑GGAACAGCACCAGGCTAATGCGGAGCCAGAAGAACAGGTGGCTCAGCGCAAAGCTGCCGt > 2:1820335/1‑150 (MQ=14) gCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCCCGCAAACG‑GGAACAGCAACAGGCTAATGCGGAGCCAGAAGAGCAGGTTGATCCGCGCAAAGCTGCAGt > 2:487328/1‑150 (MQ=14) cAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCGGCCGTCGAAGCGGCTATAGCACGCTCCAAAGCCCGCAAGCG‑GGAACAGCAAAAGCCTAATGCGGATCCAGAAGAACAGGTTGATCAGCGCAAAGCGGCAGtg > 2:689789/1‑149 (MQ=11) aaCAGGCTAATGCCGAGCCAGAACAACAGGTAGATCCGCGCAAAGCAGCAGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACG‑CGACCAGCACCCGGCTAATACCGAGCCAGAACAACAAGTAGAACAGCGCAAAGCAGCAGTCg > 1:336854/1‑150 (MQ=255) ggCTAATGCGGATCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAAc < 1:1719390/83‑1 (MQ=12) gCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCGCGCAAACG‑GGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCg > 1:1463244/1‑150 (MQ=255) aaTGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACG‑GGAACAg < 1:1652573/87‑1 (MQ=255) aaTGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACG‑GGAACAg > 2:1652573/1‑87 (MQ=255) tGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACG‑GGAAc > 1:1030854/1‑83 (MQ=255) tGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACG‑GGAAc < 2:1030854/83‑1 (MQ=255) tGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACG‑GGAACAGCAACCGGCTAATGCGGAGCCAGCAGGACAGGAAGATACGCGACCAGAAGCAGTCGAAGCGGCTAt > 1:759869/1‑150 (MQ=17) | CCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACG‑GGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTAT > NC_000913/1708705‑1708990 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |