Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,708,994 A→C 15.2% A692A (GCA→GCC rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,708,9940AC15.2% 117.0 / 2.1 77A692A (GCA→GCCrsxCSoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
Reads supporting (aligned to +/- strand):  ref base A (45/17);  new base C (10/3);  total (56/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCAAAAAAGCCGCCCAGCAGAAGGTTGTAAACGAGGACTAAA  >  NC_000913/1708847‑1709142
                                                                                                                                                   |                                                                                                                                                    
gCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGCAGCCGCTATTGCCCGCGCCAAAGCGCGCAACCGGCAACCGCAACCGGCAAATGCGGAGCCCGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTAGACGCGGCTGTTGCCcg                                                                                                                                                    >  1:1156115/1‑150 (MQ=14)
 ccAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAGCAGGTTGATCCGCGCAAAGCTGCCGTAGCCGCGGCTATTGCAcgc                                                                                                                                                   >  1:1213405/1‑150 (MQ=255)
    gAACAACAGGTCGATCCGAGCAAATCAGCCGTCGAAGCCTCTATTGCCCTAGACAAAGCTCGCAAACGTGAACATCAACCGGCTAATTCGGATCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGcc                                                                                                                                                <  2:1057478/150‑1 (MQ=21)
       caacaGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACgcgcaacc                                                                                                                                             >  1:1691896/1‑146 (MQ=255)
       caacaGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAGGCGGGGATTGCCCGCGCCaga                                                                                                                                             >  2:875701/1‑148 (MQ=255)
            ggTCGATCCTCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCTCAAACGGGAACATCAACCGGCTAATGCGGAGCCAGAAGAAAAGGTTGATCCTCTCAAAGCTGCCGTCGAAGCGTCTATTTCCCGCGCCAAAGCAca                                                                                                                                        >  1:1845048/1‑149 (MQ=17)
            ggTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAACGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTCCCGTAGCCGCGGCTATTGCCCGCGCCACAGCCCg                                                                                                                                        >  2:1338378/1‑150 (MQ=17)
             gTCGATCCGCGCAAAGTCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTAGACGCGGCTATTGCCCGCGCAAACGCACga                                                                                                                                       >  2:1769421/1‑149 (MQ=17)
               cGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAGGCGCGCAAGCGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGATGATCCGCGCAACGCAGCAGTGGCCGCGGCTATGCCGCGCGCCAACCCCCGCaa                                                                                                                                     >  1:963101/1‑150 (MQ=14)
                gATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCCCGCGCCAACGCCCGCAAg                                                                                                                                    >  1:1567947/1‑150 (MQ=255)
                gATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCCCGCAAg                                                                                                                                    >  1:451099/1‑150 (MQ=255)
                     gcgcAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCAGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGa                                                                                                                               >  1:1645512/1‑150 (MQ=255)
                       gcAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCAGTAGCAGCGGCTATTGCCCGCGCAAAGGCACGAAAGCAGGAAc                                                                                                                             >  2:400221/1‑150 (MQ=17)
                                ccgTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAgg                                                                                                                    >  1:1814063/1‑150 (MQ=255)
                                  ggcGAAGCCGCTATTTACCGAGCCAAAGCTCGCAAACGGCAACAGCAACCGGCAATTGCTGATGCAGACGCACCGGCCGATCCGCGCAAAGCAGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCt                                                                                                                  <  1:1383374/148‑1 (MQ=14)
                                  gTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCt                                                                                                                  >  1:1563701/1‑150 (MQ=255)
                                  gTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTAGCCGCGGCTATTGCCCGCGCCAACGCACGCAAACACGAACAGCAAAAggat                                                                                                                  >  2:1819028/1‑148 (MQ=14)
                                    cGAAGCCGCTATTGCCCGAGCCAAAGCTCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCAGTCGAAGCGGCTATTGCACGCGCAAAAGCACACAAGCGCGACCAGCAACAGGCTaa                                                                                                                >  2:316128/1‑150 (MQ=21)
                                     gAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCAGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAAt                                                                                                               >  1:1385812/1‑150 (MQ=255)
                                         cgtcTAATGCCTGTGACAAAGCTCGCAAACGTGTACTGTAATCGGCTAATGCCGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCgg                                                                                                           <  2:759869/147‑1 (MQ=17)
                                          cGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGACGCGGCTATTGCACGAGCCAAAGCACGCAAGCTGGCACAGCAACAGGCTAAAGCGGt                                                                                                          >  2:891364/1‑150 (MQ=255)
                                          cGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGt                                                                                                          >  1:499087/1‑150 (MQ=255)
                                             tATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCCCGCGCCCACGCCCGCAAGCTGGCACAGCAACAGGCTAATGCGGTAcc                                                                                                       >  1:1096391/1‑150 (MQ=25)
                                                 gCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCCCGCAAGCTGGAACAGCAACAGGATAATGCGGTACCagaa                                                                                                   >  2:1884501/1‑150 (MQ=255)
                                                 gCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCagaa                                                                                                   >  1:1376320/1‑150 (MQ=255)
                                                   cccaGCCAAAGCGCGCAAACGGGAACAGCCTCCGCCTTCTGCGGAGCCCGTTGATCAGGCTTATCCGCGCAAAGCCGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCagaaga                                                                                                 <  1:155902/147‑1 (MQ=17)
                                                   ccGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCagaaca                                                                                                 >  1:1762199/1‑148 (MQ=255)
                                                   ccGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTAGCAGCGGCTATTGCACGCGCCAAAGCCCAAAAGCAGGACCAGCACCAGGATATTGCGGAACCataaga                                                                                                 >  2:1772161/1‑150 (MQ=21)
                                                       gCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGACGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAGCAg                                                                                             >  2:1469397/1‑150 (MQ=255)
                                                        ccAAAGCTCCCAACCTGGAACAGCAACCGGCTAATGCGGAGCCATAACAACAGGTTTATCCGCGCATAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAgg                                                                                            <  2:610124/150‑1 (MQ=14)
                                                                  cAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGATAAAGCGGAACCAGAAGAACAGGTAGAACcgcc                                                                                  >  1:208037/1‑149 (MQ=17)
                                                                  ataaCGGCAACATCACACGGTTACTGCCGAGCCAGATGATCAGTTCTATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCcgcg                                                                                  <  2:1731380/148‑1 (MQ=17)
                                                                    aaCGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTGGGTCcgcgaa                                                                                >  2:502698/1‑148 (MQ=255)
                                                                        ggACCAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGc                                                                            <  1:875701/150‑1 (MQ=255)
                                                                        ggAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGc                                                                            >  1:1537495/1‑150 (MQ=255)
                                                                        ggAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGc                                                                            >  2:1643709/1‑150 (MQ=255)
                                                                             cgCAACCGGCTAATGCGGAGCCAGAAGTACCTGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAg                                                                       <  1:590346/149‑1 (MQ=255)
                                                                              gCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCAAAAGCACGCAAGCTGGCACAGCAACAGGCTAATGCGGTACCCGAAGAACAGGTTGATCCGCGCAAAGCGGCAGt                                                                      >  1:419229/1‑150 (MQ=255)
                                                                              ccAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCCGCTATTGCAAGCGCCAAAGCACGCAAGCAGGACCAGCAACAGGCTAATGCCGAACCAGAAGAACAGGTAACTCCGCCCATAGCGGCAGt                                                                      >  2:938663/2‑150 (MQ=37)
                                                                                aaCCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAGCAGGCTAATGCGGTACCAGAAGAAGAGGTTGATCCGCGCAAAGCGGCAGTTg                                                                    >  2:1773035/1‑150 (MQ=255)
                                                                                    ggCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGACGCGGCTATTGCACGCGCCCACGCACGCAAGCGGGCACAGCTACAGGGTAATCGCGTGCCAGAAGACCAGGTGGCTCCGCGGAGCGCGGCAGTTACcgc                                                                >  2:261858/1‑150 (MQ=21)
                                                                                       tAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGc                                                             >  1:961931/1‑150 (MQ=255)
                                                                                          cgccgAGCCAGAAGAACAGGTCGATCCGCGCACAGCAGCCGTCGACGCTGCTATTGCACGCGCCACAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTAt                                                          <  1:689789/146‑1 (MQ=17)
                                                                                              gAGCCATAACAACAGGTTGATCCGCGCAAAGCGGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGcc                                                      <  2:617773/150‑1 (MQ=255)
                                                                                                      agaaCAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCGGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCa                                              <  2:1385812/150‑1 (MQ=255)
                                                                                                       caaCAGGTCGATCCGCGATAAGCTGCATCCGAAGCCGCTATTGCACGCTCCAAAGCACGCAATCTGGAACAGCAACCGGCTAATGCGGTGCCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAg                                             <  2:1156115/149‑1 (MQ=17)
                                                                                                         aCAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCAAAAGCACGCAAGCTGGAACAGCAACCGGCTAATGCGGTACCAGAAGAACAGGTAGAACCGCGCAAAGCGGCAGTTGCCGCGGCGATTGCCCGCGCTccgcc                                           >  1:1217472/1‑146 (MQ=21)
                                                                                                            ggTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCaa                                        >  1:1650849/1‑150 (MQ=255)
                                                                                                            ggTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCaa                                        >  2:712614/1‑150 (MQ=255)
                                                                                                            ggTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCaa                                        >  1:38063/1‑150 (MQ=255)
                                                                                                            ggTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATACGCGCAAAGCGGCTGTTGCCGCGGCTATTGCCCGCGCTCAGGCCaa                                        >  1:146479/1‑150 (MQ=255)
                                                                                                               tgctCCGCTCACAGCAGCTGTCGATTCGGCTATTGCCAGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCaaaaa                                     <  1:827657/147‑1 (MQ=18)
                                                                                                                gATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCaaaaaa                                    >  1:592935/1‑150 (MQ=255)
                                                                                                                gATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCaaaaaa                                    >  2:258520/1‑150 (MQ=255)
                                                                                                                     gcgcAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCAAAAAAgccgc                               >  2:225063/1‑150 (MQ=255)
                                                                                                                       gcAAAGCTTCCGTCGAATCGGCTATTGCACGAGCCAAAGCCCGCAAGCCGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCAAAAAAGCCGccc                             <  2:1691896/150‑1 (MQ=21)
                                                                                                                       gcAAAGCGGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCAAAAAAGCCGccc                             <  2:1567947/150‑1 (MQ=255)
                                                                                                                           aGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCAAAAAAGCCGCCcagca                         <  1:400221/150‑1 (MQ=255)
                                                                                                                            gCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAAGAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCAAAAAAGCCGCCcagcag                        >  2:610255/1‑150 (MQ=255)
                                                                                                                            gCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCAAAAAAGCCGCCcagcag                        >  1:461984/1‑150 (MQ=255)
                                                                                                                              tGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGGTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCAAAAAAGCCGCCCAGCAGaa                      >  2:39211/1‑150 (MQ=255)
                                                                                                                               gCCGTCGACGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGACCAGGTTGATCCGCGCAACGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCAAACAAGCCGCCCAGCAGAAg                     >  1:1168756/1‑150 (MQ=255)
                                                                                                                               gCCGTCGAAGCGGCTATTGCACGCGCCAAAGCCCGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCATAACAGCCGCCCAGCAGAAg                     >  2:816107/1‑150 (MQ=255)
                                                                                                                               gCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCAATTGCCCGCGCTCAGGCCAAAAAAGCCGCCCAGCAGAAg                     >  1:233104/1‑150 (MQ=255)
                                                                                                                                 cGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCATCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCAAAAAAGCCGCCCAGCAGAAGGtt                  >  2:624124/1‑150 (MQ=255)
                                                                                                                                   tCGAAGCCGCTATTGCCCGCGCCAAAGCTCGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCAAAAAAGCCGCCCAGCAGAAGGTTg                 <  1:224414/150‑1 (MQ=255)
                                                                                                                                     gAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCAAAAAAGCCGCCCAGCAGAAGGTTGTa               <  2:1463244/150‑1 (MQ=255)
                                                                                                                                           ttcaTTGCCCGTGCCAAAGCTCGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTAGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCAAAAAATCCGCCCAGCAGAAGGTTGTAAACGAg         <  1:62474/147‑1 (MQ=21)
                                                                                                                                            cTATCGCTCGTGCCAAAGCTCTCAAGCTGGAACAGCAACAGTCTAATGCTGTACCAGAAGATCAGGTTGATCCGCGCAAAGCGGCAGTTGCAGCGGCTATTGCCCGCGCTCAGGCCAAAAAAGCCGCCCAGCAGAAGGTTGTAAACGAgg        <  2:123240/150‑1 (MQ=25)
                                                                                                                                             tATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCAAAAACGCCGCCCAGCAGAAGGTTGTAAACGAGGa       >  1:1364623/1‑150 (MQ=255)
                                                                                                                                              aTTGCACGAGCCAATGCTCGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCAAAAAAGCCGCCCAGCAGAAGGTTGTAAACGAGGAc      <  1:578448/150‑1 (MQ=255)
                                                                                                                                               ttGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTAGATCCGCGCAAAGCGGCAGTAGACGCGGCTATTGCCCGCGCGAAGGCCAAAAAAGCCGAACAGCAGCAGGATTTAGACGAgagat     >  2:62474/1‑146 (MQ=14)
                                                                                                                                                 gCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCAAAAAAGCCGCCCAGCAGAAGGTTGTAAACGAGGACGaa   >  2:1189835/1‑150 (MQ=255)
                                                                                                                                                  cACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCACAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCAAAAACGCCGCCCAGCAGAAGGTTGTAAACGAGGACTaaa  >  1:54754/1‑150 (MQ=255)
                                                                                                                                                  cACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCAAAAAAGCCGCCCAGCAGAAGGTTGTTAACGAGGTCTaaa  >  2:936027/1‑150 (MQ=255)
                                                                                                                                                  cACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCAAAAAAGCCGCCCAGCAGAAGGTTGTAAACGAGGACTaaa  >  2:7622/1‑150 (MQ=255)
                                                                                                                                                  cACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCAAAAAAGCCGCCCAGCAGAAGGTTGTAAACGAGGACTaaa  >  2:261977/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCAAAAAAGCCGCCCAGCAGAAGGTTGTAAACGAGGACTAAA  >  NC_000913/1708847‑1709142

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: