Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,737,435 A→G 5.2% intergenic (‑145/+21) ydhP ← / ← ynhF putative MFS transporter, inner membrane protein/stress response membrane

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,737,4350AG 5.2% 357.6 / 6.9 116intergenic (‑145/+21)ydhP/ynhFputative MFS transporter, inner membrane protein/stress response membrane
Reads supporting (aligned to +/- strand):  ref base A (57/52);  new base G (2/4);  total (59/57)
Fisher's exact test for biased strand distribution p-value = 4.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.82e-01

Only 100 of 118 total aligned reads displayed.

TGCCTTATCTCCACCTCTTCGCGTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTTTATTACCACACAAAAAGTGAT  >  NC_000913/1737290‑1737583
                                                                                                                                                 |                                                                                                                                                    
tGCCTTATCTCCACCTCTTCGCGTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAAcgcg                                                                                                                                                  >  1:23151/1‑150 (MQ=255)
  ccTTATCTCCACCTCTTCGCGTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACgcgca                                                                                                                                                >  1:629830/1‑149 (MQ=255)
        ctcCACCTCTTCGCGTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGCGCGTTCTCCCTCg                                                                                                                                          <  1:1877306/150‑1 (MQ=255)
        ctcCACCTCTTCGCGTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCg                                                                                                                                          <  2:1291942/150‑1 (MQ=255)
         tcCACCTCTTCGCGTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCga                                                                                                                                         >  1:1139693/1‑150 (MQ=255)
         tcCACCTCTTCGCGTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCga                                                                                                                                         >  2:957319/1‑150 (MQ=255)
          ccACCTCTTCGCGTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCgag                                                                                                                                        <  2:616080/150‑1 (MQ=255)
          ccACCTCTTCGCGTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCgag                                                                                                                                        >  1:517715/1‑150 (MQ=255)
           cACCTCTTCGCGTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCgaga                                                                                                                                       >  2:972654/1‑150 (MQ=255)
           cACCTCTTCGCGTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGCGAGAACGCGCTCCCTCgaga                                                                                                                                       >  2:972658/1‑150 (MQ=255)
            aCCTCTTCGCGTCATTACGCTATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAACTCAAAATAACAAAAAGAGAAAACCCTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACTCGCTCCCTCGagag                                                                                                                                      <  1:489071/150‑1 (MQ=255)
            aCCTCTTCGCGTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGagag                                                                                                                                      <  2:428802/150‑1 (MQ=255)
            aCCTCTTCGCGTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGagag                                                                                                                                      <  2:1781591/150‑1 (MQ=255)
            aCCTCTTCGCGTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGagag                                                                                                                                      <  2:8526/150‑1 (MQ=255)
             ccTCTTCGCGTCATTACGCTATATTCATTAAAGTGGCGAAATCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGCGCGTTCTCCCTCgcatct                                                                                                                                     <  1:1655679/150‑6 (MQ=255)
             ccTCTTCGCGTCATTACGCGATATTCATTAAAGTGGCGAAAGAATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAgg                                                                                                                                     <  1:993872/150‑1 (MQ=255)
              ctctTCGCGTCATTACGCGATATTCGTTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGCGCGTTCTCCCTCgcatctc                                                                                                                                    <  2:13504/150‑7 (MQ=255)
              ctctTCGCGTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGa                                                                                                                                    <  1:1038734/150‑1 (MQ=255)
               tctTCGCGTCATTACGCGATATTCTTTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGaa                                                                                                                                   >  1:895801/1‑150 (MQ=255)
               tctTCGCGTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGaa                                                                                                                                   >  1:1583370/1‑150 (MQ=255)
                 ttCGCGTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAAt                                                                                                                                 >  2:1226752/1‑150 (MQ=255)
                   cgcgTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCa                                                                                                                               >  1:43173/1‑150 (MQ=255)
                   cgcgTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCa                                                                                                                               >  2:69904/1‑150 (MQ=255)
                     cgTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCATCGAGAGGAAATCAGt                                                                                                                             >  1:573080/1‑150 (MQ=255)
                         aTTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAg                                                                                                                         <  2:1589337/150‑1 (MQ=255)
                                 atatTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAg                                                                                                                 <  2:378505/150‑1 (MQ=255)
                                 atatTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAg                                                                                                                 <  1:1251761/150‑1 (MQ=255)
                                    ttCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCa                                                                                                              <  1:1446940/150‑1 (MQ=255)
                                     tCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCaa                                                                                                             >  1:1392582/1‑150 (MQ=255)
                                      cATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGCACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCaaa                                                                                                            >  2:187831/1‑150 (MQ=255)
                                      cATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCaaa                                                                                                            <  1:957319/150‑1 (MQ=255)
                                       aTTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAATTCAGTGCAGCGCGGCAGTCAAAc                                                                                                           >  1:1676610/1‑150 (MQ=255)
                                       aTTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAAc                                                                                                           >  1:681808/1‑150 (MQ=255)
                                       aTTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGACAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTAGAGAGTAAATCAGTGCAGCGCGGCAGTCAAAc                                                                                                           >  1:1225419/1‑150 (MQ=255)
                                           aaGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGAAGCGCGGCAGTCAAACCCAc                                                                                                       >  2:298228/1‑150 (MQ=255)
                                             gTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACgg                                                                                                     <  1:368857/150‑1 (MQ=255)
                                               ggCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCt                                                                                                   >  2:483024/1‑150 (MQ=255)
                                                gCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCAACGGCTa                                                                                                  >  2:1824229/1‑150 (MQ=255)
                                                 cGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTAc                                                                                                 <  1:781457/150‑1 (MQ=255)
                                                  gAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGCTGCGAGGGAGAACGCGCTCCCTCGAGAGGAATTCAGTGCAGCGCGGCAGTCAAACCCACGGCTACg                                                                                                <  2:1225844/150‑1 (MQ=255)
                                                     aGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATc                                                                                             <  1:723053/150‑1 (MQ=255)
                                                      gCATTACAGAAATCACAAAAAAATCAAAATAACAAAAAGAGAAAACACTTTTTCCTTTTTGCTAACAAACAGTAAGGAGATGCGAGGGAGAACGAGCTCCCTCGAGAGGAAATCAGAGCAGCGCGTCAGTCAAACCCACGGCTACTATCa                                                                                            <  2:307163/150‑1 (MQ=255)
                                                       cATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCaa                                                                                           >  1:1593446/1‑150 (MQ=255)
                                                         tGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAAc                                                                                         <  1:1641451/150‑1 (MQ=255)
                                                         tGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCGACGATCAAAc                                                                                         >  2:1196729/1‑150 (MQ=255)
                                                          gACAGCAATCACAAAAAAATTAAAATAACAAAAAGAGAAATCACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCTGCGCGGCAGTCAAACCCACGGCTACGATCAAAcc                                                                                        <  1:354040/150‑1 (MQ=255)
                                                          gACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAAcc                                                                                        >  2:1582163/1‑150 (MQ=255)
                                                           aCAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCg                                                                                       >  2:725469/1‑150 (MQ=255)
                                                            cAGCAATCACAAAAAAATGAAAATAAAAAAAAGAGAAAACACTTTTGCCATTTTGTTAACAAACAGGAAGGAGATGCGAGGGAGACCGCGCTGCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGa                                                                                      <  2:1213416/150‑1 (MQ=255)
                                                              gCAATCACAAAAAAATGAAAATAACAACAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAgg                                                                                    <  1:980686/150‑1 (MQ=255)
                                                              gCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTACCAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCTCGGCAGTCAACCCCACGGCTACGATCAAACCGAgg                                                                                    >  2:768016/1‑150 (MQ=255)
                                                              gCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAgg                                                                                    >  1:827094/1‑150 (MQ=255)
                                                                   cacaAAAAAATGAAAATAACAGAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGAt                                                                               >  2:715132/1‑150 (MQ=255)
                                                                      aaaaaaaTGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGATAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAATCCCACGGCTACGATCAAACCGAGGACGATAAt                                                                            >  1:1431749/1‑150 (MQ=255)
                                                                       aaaaaaTGAAAATAACAAAAAGAGAAAACCCTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGACATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGAGCAACCCGAGGACGATAATc                                                                           >  1:1222992/1‑150 (MQ=255)
                                                                        aaaaaTGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCg                                                                          <  2:79673/150‑1 (MQ=255)
                                                                         aaaaTGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCgt                                                                         <  1:1213838/150‑1 (MQ=255)
                                                                           actGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCgttg                                                                       <  2:332234/148‑1 (MQ=255)
                                                                           aaTGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCgttg                                                                       <  2:1660593/150‑1 (MQ=255)
                                                                              taaaaTAACATAATGAGAAAACACTTTTGCCATTTTGCTAAAATACAGGAAGGAGATTCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGTCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTa                                                                    <  1:1172675/149‑1 (MQ=255)
                                                                               aaaaTAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTAc                                                                   >  1:885724/1‑150 (MQ=255)
                                                                                aaaTAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTAcc                                                                  >  1:1383082/1‑150 (MQ=255)
                                                                                aaaTAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTAcc                                                                  >  2:613229/1‑150 (MQ=255)
                                                                                 aaTAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCa                                                                 <  2:542079/150‑1 (MQ=255)
                                                                                  aTAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAg                                                                <  1:1852592/150‑1 (MQ=255)
                                                                                      cAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGaa                                                            <  1:491468/150‑1 (MQ=255)
                                                                                          aactgaAAACACTTTTGCCATTTTTCTAACAAACAGGAAGGTGATGCGACGGATAACGCGCTCCCTCGAGAGGTAATCAGTGCAGCGCGGCAGTCAAACCCAAGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAAtt                                                        <  1:1812245/146‑1 (MQ=255)
                                                                                          aaGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAAtt                                                        <  2:517715/150‑1 (MQ=255)
                                                                                           agagAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAAttt                                                       >  1:1098861/1‑150 (MQ=255)
                                                                                             agaAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTaa                                                     <  1:192906/150‑1 (MQ=255)
                                                                                             agaAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTaa                                                     <  2:681808/150‑1 (MQ=255)
                                                                                              gaAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAg                                                    <  1:1872729/150‑1 (MQ=255)
                                                                                                   cacTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTAACAGTGAAAATTTAAGGTCgg                                               >  1:791198/1‑150 (MQ=255)
                                                                                                         tGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCtca                                         >  2:611195/1‑150 (MQ=255)
                                                                                                            cATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCtcatca                                      >  1:1500621/1‑150 (MQ=255)
                                                                                                            cATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCtcatca                                      >  2:227167/1‑150 (MQ=255)
                                                                                                            cATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCtcatca                                      >  2:1496259/1‑150 (MQ=255)
                                                                                                            cATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCtcatca                                      >  1:821839/1‑150 (MQ=255)
                                                                                                             aTTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCaa                                     >  2:1057092/1‑150 (MQ=255)
                                                                                                                 tGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGttt                                 <  1:1730118/150‑1 (MQ=255)
                                                                                                                   cTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGCCGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTtc                               >  2:159518/1‑150 (MQ=255)
                                                                                                                   cTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTtc                               >  2:529465/1‑150 (MQ=255)
                                                                                                                   cTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTtc                               >  1:440044/1‑150 (MQ=255)
                                                                                                                    tAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTtct                              >  1:1170242/1‑150 (MQ=255)
                                                                                                                     aacaaacaGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTtctc                             >  2:1135425/1‑150 (MQ=255)
                                                                                                                          acaGGAAGGATATGCGATGGAGAACGCGCTCCATCGAGATGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACTATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCtttt                        <  1:997169/150‑1 (MQ=255)
                                                                                                                           caGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCttttt                       <  2:1375413/150‑1 (MQ=255)
                                                                                                                           caGGAAGGAGATGCGAGGGAGAACGCGCTCCCGCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCttttt                       <  1:1475648/150‑1 (MQ=255)
                                                                                                                            cggaaggaGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCtttttt                      <  2:869217/149‑1 (MQ=255)
                                                                                                                             ggcaggaGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCACCGCGGCAGTCAAACCCACGGCTACGAACAAACCGAGTACGATAATCGTTGTTACCAGTGAAAATTTAAGGGCGGTGCTCATCAAGTTTTCTCCTTTTtta                     <  2:1158963/147‑1 (MQ=255)
                                                                                                                             ggaaggaGATGCTAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGAGAATCGTTGTTACCAGTGAAAATTAAAGGTAGGTGCTCATCAAGTTTTCTCCTTTTtta                     >  1:1068122/1‑150 (MQ=255)
                                                                                                                             ggaaggaGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTtta                     >  2:1085925/1‑150 (MQ=255)
                                                                                                                                  gagaTGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTTTATTAcc                <  2:43173/150‑1 (MQ=255)
                                                                                                                                    gaTGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCACACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTTTATTACcac              >  2:1584611/1‑150 (MQ=255)
                                                                                                                                    gaTGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTTTATTACcac              <  2:646375/150‑1 (MQ=255)
                                                                                                                                    gaTGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTTTATTACcac              >  2:18912/1‑150 (MQ=255)
                                                                                                                                     tctcGAGGGAGCGCGTTCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTTTATTACcacc             >  2:322418/4‑149 (MQ=255)
                                                                                                                                      ctcGAGGGAGTGCGTTCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCCTCAAGTTTTCTCCTTTTTTATTACcacac            >  1:882472/3‑150 (MQ=255)
                                                                                                                                               gaACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTTTATTACCACACAAAAAGTGa   <  2:1388807/150‑1 (MQ=255)
                                                                                                                                                aaCGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTTTATTACCACACAAAAAGTGat  >  2:605297/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
TGCCTTATCTCCACCTCTTCGCGTCATTACGCGATATTCATTAAAGTGGCGAAAGCATGACAGCAATCACAAAAAAATGAAAATAACAAAAAGAGAAAACACTTTTGCCATTTTGCTAACAAACAGGAAGGAGATGCGAGGGAGAACGCGCTCCCTCGAGAGGAAATCAGTGCAGCGCGGCAGTCAAACCCACGGCTACGATCAAACCGAGGACGATAATCGTTGTTACCAGTGAAAATTTAAGGTCGGTGCTCATCAAGTTTTCTCCTTTTTTATTACCACACAAAAAGTGAT  >  NC_000913/1737290‑1737583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑