Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,770,203 T→A 6.8% intergenic (+17/‑169) ydiK → / → rprA UPF0118 family inner membrane protein/sRNA antisense activator for RpoS translation, Hfq‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,770,2030TA 6.8% 305.8 / 8.5 116intergenic (+17/‑169)ydiK/rprAUPF0118 family inner membrane protein/sRNA antisense activator for RpoS translation, Hfq‑dependent
Reads supporting (aligned to +/- strand):  ref base T (60/47);  new base A (3/5);  total (63/53)
Fisher's exact test for biased strand distribution p-value = 4.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.94e-01

Only 100 of 121 total aligned reads displayed.

GTTTATTGGTCCGGTTCTGTTAGCCGTTTCCTGGCGTCTGTTTGCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCAAAAAGTCC  >  NC_000913/1770054‑1770352
                                                                                                                                                     |                                                                                                                                                     
gTTTATTGGTCCGGTTCTGTTAGCCGTTTCCTGGCGTCTGTTTGCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGct                                                                                                                                                       >  1:938735/1‑150 (MQ=255)
gTTTATTGGTCCGGTTCTGTTAGCCGTTTCCTGGCGTCTGTTTGCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATATCTCATCAGGCGGct                                                                                                                                                       >  2:170422/1‑150 (MQ=255)
gTTTATTGGTCCGGTGCTGTTAGCCGTTTCCTGGCGTCTGTTTGCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGct                                                                                                                                                       <  2:1323216/150‑1 (MQ=255)
   tATTGGTCCGGTTCTGTTAGCCGTTTCCTGGCGTCTGTTTGCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTg                                                                                                                                                    >  2:236972/1‑150 (MQ=255)
     ttGGTCCGGTTCTGTTAGCCGTTTCCTGGCGTCTGTTTGCCGCGTGTGTGGAAGAAGTCCCACCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTgcc                                                                                                                                                  >  1:1259405/1‑150 (MQ=255)
     ttGGTCCGGTTCTGTTAGCCGTTTCCTGGCGTCTGTTTGCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAAACGAATAAGTAATTTCTCATCAGGCGGCTCTgcc                                                                                                                                                  <  2:1723181/150‑1 (MQ=255)
       ggTCCGGTTCTGTTAGCCGTTTCCTGGCGTCTGTTTGCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGCCCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTgacgc                                                                                                                                                >  2:967278/1‑150 (MQ=255)
       ggTCCGGTTCTGTTAGCCGTTTCCTGGCGTCTGTTTGCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTgccgc                                                                                                                                                >  1:438588/1‑150 (MQ=255)
        gTCCGGTTCTGTTAGCCGTTTCCTGGCGTCTGTTTGCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTgccgcc                                                                                                                                               >  2:7232/1‑150 (MQ=255)
        gTCCGGTTCTGTTAGCCGTTTCCTGGCGTCTGTTTGCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTgccgcc                                                                                                                                               <  2:1515344/150‑1 (MQ=255)
        gTCCGGTTCTGTTAGCCGTTTCCTGGCGTCTGTTTGCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTgccgcc                                                                                                                                               >  1:453310/1‑150 (MQ=255)
        gTCCGGTTCTGTTAGCCGTTTCCTGGCGTCTGTTTGCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTgccgcc                                                                                                                                               >  2:880117/1‑150 (MQ=255)
          ccGGTTCTGTTAGCCGTTTCCTGGCGTCTGTTTGCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTg                                                                                                                                             >  2:1722813/1‑150 (MQ=255)
           cGGTTCTGTTAGCCGTTTCCTGGCGTCTGTTTGCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTTATCAGGCGGCAGAGCCGCCTGa                                                                                                                                            <  1:1780440/150‑1 (MQ=255)
           cGGGTCTGTTAGCCGTTTCCTGGCGTCTGTTTGCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCAGAGCCGCCTGa                                                                                                                                            <  2:168992/150‑1 (MQ=255)
            ggTTCTGTTAGCCTTTTCCTGGCGTCTGTTTGCCGCGTGGTTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCAGAGCCGCCTGAt                                                                                                                                           <  1:759327/150‑1 (MQ=255)
             gtgcTGTTAGCCGTTTCCTGGCGTCTGTTTGCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGAtt                                                                                                                                          <  1:652059/147‑1 (MQ=255)
               tCTGTTAGCCGTTTCCTGGCGTCTGTTTGCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGt                                                                                                                                        <  2:1643556/150‑1 (MQ=255)
                cTGTTAGCCGGTTCCTGGCGTCTGTTTGCCGCGGGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGTAATCGAGATACCGAATATGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGtt                                                                                                                                       <  1:941121/150‑1 (MQ=255)
                 tGTTAGCCGTTTCCTGGCTTCTGTTTGCCTCGTGGGTGGAAGAAGTCCCGCCGCCGACGTACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCTAATAAGTAATTTCTCATCAGGCGGCTCTTCCGCCTGTTTGTTa                                                                                                                                      <  2:1689865/150‑1 (MQ=255)
                  gTTAGCCGTTTCCTGGCGTCTGTTTGCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTaa                                                                                                                                     <  1:257128/150‑1 (MQ=255)
                  gTTAGCCGTTTCCTGGCGTCTGTTTGCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTaa                                                                                                                                     >  1:302022/1‑150 (MQ=255)
                      gCCGTTTCCTGGCGTCTGTTTGCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAcccac                                                                                                                                 >  2:1886662/1‑150 (MQ=255)
                      gCCGTTTCCTGGCGTCTGTTTGCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAaccac                                                                                                                                 >  2:45156/1‑150 (MQ=255)
                      gCCGTTTCCTGGCGTCTGTTTGCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAaccaa                                                                                                                                 >  2:1545322/1‑149 (MQ=255)
                       ccGTTTCCTGGCGTCTGTTTGCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAaccacc                                                                                                                                <  1:196585/150‑1 (MQ=255)
                         gTTTCCTGGCGTCTGTTTGCCGCGTGGGGGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGc                                                                                                                              <  2:814975/150‑1 (MQ=255)
                             ccTGGCGTCTGTTTGCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAAt                                                                                                                          >  2:1374857/1‑150 (MQ=255)
                              cTGGCGTCTGTTTGCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAAtt                                                                                                                         >  2:79501/1‑150 (MQ=255)
                               tGGCGTCTGTTTGCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATCAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTg                                                                                                                        <  2:696471/150‑1 (MQ=255)
                                 gCGTCTGTTTGCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGACGCCTGATTGTTAACCACCGCTAATTGat                                                                                                                      >  1:782604/1‑150 (MQ=255)
                                  cGTCTGTTTGCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGata                                                                                                                     <  2:913622/150‑1 (MQ=255)
                                      tGTTTGCCGCGTGGGTGGAAGAAGTCCCGCCGCCGCCGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACAACCGCTAATTGATATttg                                                                                                                 >  2:1840184/1‑150 (MQ=255)
                                       gTTTGCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGCCGAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGGAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCAGCGCTAAGTGATAtgtgg                                                                                                                >  2:290039/1‑149 (MQ=255)
                                       gTTTGCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGCTTGTTAACCACCGCTAATTGATATttgc                                                                                                                >  1:22702/1‑150 (MQ=255)
                                             cgcgTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGctc                                                                                                          <  2:302022/150‑1 (MQ=255)
                                              gcgTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTACTTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGctct                                                                                                         <  2:1372901/150‑1 (MQ=255)
                                                  gggtggAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGTAATTGTCGAAGATCTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGAGGCTCTGCCGGCTGATTGTTAACGACCGCTAATTGATTTTTGCTTGCTCTTgcc                                                                                                     >  1:1715271/1‑150 (MQ=255)
                                                  gggtggAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTccc                                                                                                     >  1:1628846/1‑150 (MQ=255)
                                                  gggtggAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTccc                                                                                                     >  1:739834/1‑150 (MQ=255)
                                                    gtggAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCa                                                                                                   <  2:1754903/150‑1 (MQ=255)
                                                     tggAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACAGCTAATTGATATTTGCTTGCTCTTCCCCag                                                                                                  >  1:124005/1‑149 (MQ=255)
                                                      ggAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCatt                                                                                                 >  1:694056/1‑149 (MQ=255)
                                                      ggAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCata                                                                                                 >  1:361611/1‑150 (MQ=255)
                                                           aagTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTc                                                                                            <  2:1037509/150‑1 (MQ=255)
                                                            agTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGATAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTcc                                                                                           >  2:938206/1‑150 (MQ=255)
                                                            agTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTcc                                                                                           <  1:152665/150‑1 (MQ=255)
                                                            agTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTcc                                                                                           >  1:1589963/1‑150 (MQ=255)
                                                            agTCCCGCCGCCGACGGACCAAACTGAAGAAAATCTCGAAGAACATGGCGAAAACGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTCGCTTGCTCTTCCCCATATTTTcc                                                                                           <  1:315294/150‑1 (MQ=255)
                                                               cccGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGc                                                                                        <  2:490764/150‑1 (MQ=255)
                                                                 cgccgccgACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCtt                                                                                      >  1:1058214/1‑150 (MQ=255)
                                                                       cgACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGcc                                                                                <  2:672349/150‑1 (MQ=255)
                                                                       cgACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGcc                                                                                >  2:332044/1‑150 (MQ=255)
                                                                         aCGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCACGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCAt                                                                              <  2:1541850/150‑1 (MQ=255)
                                                                           ggACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAATTCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTc                                                                            <  1:272640/150‑1 (MQ=255)
                                                                             aCCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAg                                                                          >  2:188915/1‑150 (MQ=255)
                                                                              ccAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCTCCATTCAGc                                                                         >  2:1832368/1‑150 (MQ=255)
                                                                              ccAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGc                                                                         >  1:1095008/1‑150 (MQ=255)
                                                                              ccAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGc                                                                         <  1:807942/150‑1 (MQ=255)
                                                                                aaCCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTg                                                                       <  2:627024/150‑1 (MQ=255)
                                                                                  ccGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGgt                                                                     >  2:56131/1‑150 (MQ=255)
                                                                                  acGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGgt                                                                     <  1:988343/149‑1 (MQ=255)
                                                                                   cGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGgta                                                                    >  2:1157989/1‑150 (MQ=255)
                                                                                        gaaCTTCTCGCAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTAcc                                                               <  1:973479/150‑1 (MQ=255)
                                                                                        gaaATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTAcc                                                               <  2:361611/150‑1 (MQ=255)
                                                                                            ttCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGACTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTc                                                           <  2:745634/150‑1 (MQ=255)
                                                                                                  aagaaCTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAAt                                                     <  1:880117/150‑1 (MQ=255)
                                                                                                  aagaaCTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATAAGGCGGCTCTGCCGCCTGATTGTTAACCTCAGCAAATTGATATATGCTTGATCTTCACCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAAt                                                     >  1:1412088/1‑150 (MQ=255)
                                                                                                      aCTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCtt                                                 <  1:1722813/150‑1 (MQ=255)
                                                                                                       cTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCttt                                                >  1:172711/1‑150 (MQ=255)
                                                                                                       cTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACAGCTAATTGATATTTGCTTGCTCTTCCACATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCttt                                                >  1:1098545/1‑150 (MQ=255)
                                                                                                       attGGCGAAATCGAGAAACCGAATAAGTAATTTGTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATTTTTGCTTGCTCTTCCCCTTATTTTCCTGCTTACCGCCATTCTGCTGGTAGTTCCTGTCGCAAATTCttt                                                <  1:402202/149‑1 (MQ=255)
                                                                                                          ggCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACa                                             <  1:34884/150‑1 (MQ=255)
                                                                                                            cGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGAAAATTCTTTACAGt                                           >  2:57447/1‑150 (MQ=255)
                                                                                                               aaTCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGtttt                                        >  1:396532/1‑150 (MQ=255)
                                                                                                                 tCGAGAAACCGAATAAGTAATTTCTCATCAAGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTa                                      >  2:1639775/1‑150 (MQ=255)
                                                                                                                  cGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCTCAAATTCTTTACAGTTTTTaa                                     >  1:1843424/1‑150 (MQ=255)
                                                                                                                  cGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTaa                                     >  1:383344/1‑150 (MQ=255)
                                                                                                                   gagaAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTaaa                                    >  1:279819/1‑150 (MQ=255)
                                                                                                                     gaAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTATCTTGATCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGACGCAAATTCTTAACAGTTTTTAAACt                                  >  2:750000/1‑150 (MQ=255)
                                                                                                                       aaCCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCATCCCCATAGTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTaa                                >  2:306915/1‑150 (MQ=255)
                                                                                                                        aCCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGACTGATTGTTAACCACCGCTAATTGATTTTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTatt                               >  2:101082/1‑148 (MQ=255)
                                                                                                                        aCCGAATAAGTAATTTCTCATCAGGCGGCGCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAAt                               <  1:1650246/150‑1 (MQ=255)
                                                                                                                         ccGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATg                              >  1:1528535/1‑150 (MQ=255)
                                                                                                                               aaGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACAACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGa                        >  1:923819/1‑150 (MQ=255)
                                                                                                                                aGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACTGTTTTTAAACTAATGAGACGaa                       >  2:133086/1‑150 (MQ=255)
                                                                                                                                       tctcATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATc                <  2:1213216/150‑1 (MQ=255)
                                                                                                                                       tctcATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATc                <  1:1374857/150‑1 (MQ=255)
                                                                                                                                        ctcATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCg               <  1:864662/150‑1 (MQ=255)
                                                                                                                                        acaatcaGGCGGCAGAGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCg               >  1:866083/4‑150 (MQ=255)
                                                                                                                                         caatcaGGCGGCAGAGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGgt                                                                     >  2:13814/3‑95 (MQ=39)
                                                                                                                                         caatcaGGCGGCAGAGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGgt                                                                     <  1:13814/93‑1 (MQ=39)
                                                                                                                                          catcaGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGAc             >  2:1551572/1‑150 (MQ=255)
                                                                                                                                           atcaGGCGTCTCTTCCGCATGATTTTTAACCACCGCTAATTGATATATTCTTGATATTCACAATATTTTCCTGCTTAACGCAATTCAGCTGTTAGTACCTGTCGCAAATTCTTTACATTTTTAAAACTAATGAGACGAATCTGATCGACg            <  1:161873/150‑1 (MQ=255)
                                                                                                                                            tcaGGCGGCTCTGCGGCCTGATTGTTAACCACCGCTACTTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGc           <  2:938735/150‑1 (MQ=255)
                                                                                                                                             caGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGAcaca          >  1:976166/1‑150 (MQ=255)
                                                                                                                                               ggcggcTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCaaa        >  2:428549/1‑150 (MQ=255)
                                                                                                                                               ggcggcTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCaaa        >  2:205562/1‑150 (MQ=255)
                                                                                                                                                     tctGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCAAAAAGTcc  <  1:1639775/150‑1 (MQ=255)
                                                                                                                                                     tctGCCGCCTGATTGTTAACCAACGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCAAAAAGTcc  <  2:232621/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GTTTATTGGTCCGGTTCTGTTAGCCGTTTCCTGGCGTCTGTTTGCCGCGTGGGTGGAAGAAGTCCCGCCGCCGACGGACCAACCGGAAGAAATTCTCGAAGAACTTGGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGGTAGTACCTGTCGCAAATTCTTTACAGTTTTTAAACTAATGAGACGAATCTGATCGACGCAAAAAGTCC  >  NC_000913/1770054‑1770352

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: