Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,792,241 A→T 6.3% intergenic (‑221/+26) ydiV ← / ← nlpC anti‑FlhD4C2 factor, inactive EAL family phosphodiesterase/putative C40 clan peptidase lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,792,2410AT 6.3% 316.2 / 6.0 110intergenic (‑221/+26)ydiV/nlpCanti‑FlhD4C2 factor, inactive EAL family phosphodiesterase/putative C40 clan peptidase lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (46/55);  new base T (3/5);  total (49/61)
Fisher's exact test for biased strand distribution p-value = 7.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 116 total aligned reads displayed.

TACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAATACCTACA  >  NC_000913/1792092‑1792387
                                                                                                                                                      |                                                                                                                                                  
tACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTa                                                                                                                                                    >  2:1171188/1‑150 (MQ=255)
tACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTa                                                                                                                                                    >  2:1598540/1‑150 (MQ=255)
tACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTa                                                                                                                                                    <  2:1510983/150‑1 (MQ=255)
 aCAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTat                                                                                                                                                   <  1:815802/150‑1 (MQ=255)
    gAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTatcat                                                                                                                                                <  2:1368084/150‑1 (MQ=255)
       ggAGTAAGTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGc                                                                                                                                             <  2:947182/150‑1 (MQ=255)
       ggAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGc                                                                                                                                             <  2:1874778/150‑1 (MQ=255)
         aGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATTGACGGCGATGTATCATCGCCg                                                                                                                                           >  2:439740/1‑150 (MQ=255)
           tAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGATACATCGCCGTc                                                                                                                                         <  1:1467624/150‑1 (MQ=255)
           tAACTATCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTc                                                                                                                                         <  1:888298/150‑1 (MQ=255)
           aaaCTTTCATTTGTTCCATGAGAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTc                                                                                                                                         <  1:1207497/149‑1 (MQ=255)
            aaCTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTcc                                                                                                                                        >  1:729768/1‑150 (MQ=255)
              cTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCAt                                                                                                                                      >  1:414868/1‑150 (MQ=255)
              cTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGATACATCGCCGTCCAt                                                                                                                                      <  2:1380714/150‑1 (MQ=255)
               tttCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCAtt                                                                                                                                     <  2:18770/150‑1 (MQ=255)
                ttCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCAttt                                                                                                                                    >  2:1685419/1‑150 (MQ=255)
                ttCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGAGATGTATCATCGCCCTCCAttt                                                                                                                                    >  1:853810/1‑150 (MQ=255)
                   aTTTGTTCCATGTTAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATCATTATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTc                                                                                                                                 <  1:808568/150‑1 (MQ=255)
                    tttGTTCCATGTTAACCACTTTTTCATGTGTCCTTTTTTTAGATTATCCTGATTATACACGCATAATCCATTTACGGAATTTTTGTCTGCAAAATACTCCTGTCTTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCg                                                                                                                                <  1:219357/150‑1 (MQ=255)
                     ttGTTCCATGTTAACCACTTTTTCAGGGTTCCTTTTTTTAGTTTATCCTGATTATAAACGAATAATCCATTTACGTAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGa                                                                                                                               <  1:746178/150‑1 (MQ=255)
                      tGTTCCATGTTAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGAc                                                                                                                              <  2:1224315/150‑1 (MQ=255)
                      tGTTCCATGTTAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGAc                                                                                                                              <  2:477003/150‑1 (MQ=255)
                       gTTCCATGTTAACCACTTTTTCAGGGGTTCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACa                                                                                                                             >  2:27418/1‑150 (MQ=255)
                          ccATGTTAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGCCATAc                                                                                                                          >  2:959296/1‑150 (MQ=255)
                            aTGTTAACCACTTTTTCAGGGGTCCTTTTTTTCGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTa                                                                                                                        <  1:138319/150‑1 (MQ=255)
                            aTGTTAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTa                                                                                                                        <  1:1367154/150‑1 (MQ=255)
                                tAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGAtt                                                                                                                    >  2:1606213/1‑150 (MQ=255)
                                 aaCCACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTc                                                                                                                   >  2:1748671/1‑150 (MQ=255)
                                 aaCCACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTc                                                                                                                   >  2:1592349/1‑150 (MQ=255)
                                  aCCACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCg                                                                                                                  <  1:1558827/150‑1 (MQ=255)
                                    cACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGtc                                                                                                                <  2:1617006/150‑1 (MQ=255)
                                    cACTTTTTCAGGGGTCCTGTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGACTGCAAAATACTACTGTATTAAGGAGTAAATGGACGGCGATGTATCATCGCAGTCCATATTTCGACATACTAGATTCGtc                                                                                                                >  2:8055/1‑150 (MQ=255)
                                      cTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTATCAAAATAAGTCGTGTTTTCAGGATTAATTGGACGGCGATGTTGCATCTCAGTCCATTTTTGGCCATACTAGATTCGtctc                                                                                                              >  2:76528/1‑150 (MQ=255)
                                         tttCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGcc                                                                                                           <  2:199113/150‑1 (MQ=255)
                                          ttCAGGGTTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCt                                                                                                          <  2:562670/150‑1 (MQ=255)
                                           tCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAAAAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTg                                                                                                         >  2:1125938/1‑150 (MQ=255)
                                             aGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGcc                                                                                                       <  2:1624338/150‑1 (MQ=255)
                                             aGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGcc                                                                                                       <  2:1072668/150‑1 (MQ=255)
                                              tgggTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCa                                                                                                      <  1:240517/149‑1 (MQ=255)
                                              ggggTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAg                                                                                                     >  2:1547258/1‑150 (MQ=255)
                                               gggTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAg                                                                                                     <  1:1834002/150‑1 (MQ=255)
                                               gggTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAg                                                                                                     <  2:663666/150‑1 (MQ=255)
                                                  tCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGaaa                                                                                                  >  2:1044152/1‑150 (MQ=255)
                                                  tCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTAAGGAATTTTTGTCTGCTAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCTTTTTTCGACATACTAGATTCGTCTCGCCTGCCAGaaa                                                                                                  <  2:916857/150‑1 (MQ=255)
                                                    cTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAAtt                                                                                                >  2:103539/1‑150 (MQ=255)
                                                     tttttttAGAGTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAAttt                                                                                               >  1:1007841/1‑150 (MQ=255)
                                                          ttAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTg                                                                                          <  2:916334/150‑1 (MQ=255)
                                                          ttAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTg                                                                                          <  1:952126/150‑1 (MQ=255)
                                                            aGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGcc                                                                                        <  2:918055/150‑1 (MQ=255)
                                                                   ccTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGAc                                                                                 <  1:453851/150‑1 (MQ=255)
                                                                    cTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACa                                                                                <  1:20266/150‑1 (MQ=255)
                                                                    atGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACa                                                                                <  2:1010012/149‑1 (MQ=255)
                                                                     tGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACAt                                                                               <  2:452134/150‑1 (MQ=255)
                                                                      gATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACAtt                                                                              <  2:356778/150‑1 (MQ=255)
                                                                           taAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCaa                                                                         >  2:791787/1‑150 (MQ=255)
                                                                            aaaCGAATACTCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATTGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAg                                                                        <  2:919051/150‑1 (MQ=255)
                                                                            aaaCGAATAATCCATTTACGGAATTTTTGTCTTCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAg                                                                        <  1:425546/150‑1 (MQ=255)
                                                                            aaaCGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGTTTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAg                                                                        >  1:1729382/1‑150 (MQ=255)
                                                                             aaCGAATAATCCATTTACGGAATTTTTGTCTGTAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATAAAGt                                                                       >  2:837129/1‑150 (MQ=255)
                                                                             aaCGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGt                                                                       >  2:1348632/1‑150 (MQ=255)
                                                                              aCGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTg                                                                      >  2:1089384/1‑150 (MQ=255)
                                                                              aCGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTg                                                                      <  1:1054835/150‑1 (MQ=255)
                                                                                 aataatCCATTTACGGAATTTTTGTCTGAAAATTACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAgg                                                                   <  1:358440/150‑1 (MQ=255)
                                                                                       ccATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCa                                                             <  1:1838748/150‑1 (MQ=255)
                                                                                        cATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGACCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCAt                                                            >  2:505755/1‑150 (MQ=255)
                                                                                        aaTTTACGGAATTTTTGTCTGAAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCAt                                                            <  1:1594699/149‑1 (MQ=255)
                                                                                         aTTTACGGTATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCTCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGACAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATc                                                           <  1:79977/150‑1 (MQ=255)
                                                                                          tttACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCa                                                          >  2:270271/1‑150 (MQ=255)
                                                                                               ggAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCGAATAGACATTATCAAGTGAGGAACGCATCACTccc                                                     >  1:1003940/1‑150 (MQ=255)
                                                                                               ggAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTAGTCTCGCCTGCCAGAAATTTTTCTGCCAATTGACATTATCAATTGATGAACGCATCaaaccc                                                     >  1:1834649/1‑145 (MQ=255)
                                                                                                       tGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGt                                             <  2:274116/150‑1 (MQ=255)
                                                                                                            gCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTTCCAATAGACATTATCAAGTGATGAACGCATCAATCCCTTGCTTGTAGAgg                                        >  1:1677111/1‑150 (MQ=255)
                                                                                                            gCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTTGTAGAgg                                        >  2:215050/1‑150 (MQ=255)
                                                                                                               aaaTACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCGTGCTGGTAGAGGCGt                                     >  2:1430746/1‑150 (MQ=255)
                                                                                                                aaTACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTg                                    <  2:1418230/150‑1 (MQ=255)
                                                                                                                  tactacTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGa                                  >  2:405687/1‑150 (MQ=255)
                                                                                                                    actactGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGAt                                 <  1:103539/150‑1 (MQ=255)
                                                                                                                       actGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATaaa                              >  1:318052/1‑150 (MQ=255)
                                                                                                                       actGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTACCTTGCTGGTAGAGGCGTGGATaaa                              >  2:1425504/1‑150 (MQ=255)
                                                                                                                        ctGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAATGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAAt                             >  2:236257/1‑150 (MQ=255)
                                                                                                                           tATTCAGGAGTATATGGACGGCGATGTTTCATCGCCGTCCATTTTTCGACATACTATATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTgg                          <  2:567689/150‑1 (MQ=255)
                                                                                                                            aTTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGgt                         <  1:570968/150‑1 (MQ=255)
                                                                                                                            aTTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGgt                         <  2:1905561/150‑1 (MQ=255)
                                                                                                                              tCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGgttg                       >  2:1325261/1‑150 (MQ=255)
                                                                                                                               cAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGgttgt                      >  1:524115/1‑150 (MQ=255)
                                                                                                                                aGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGgttgtt                     <  2:1871743/150‑1 (MQ=255)
                                                                                                                                     tAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTAt                >  2:728993/1‑150 (MQ=255)
                                                                                                                                      aaaTGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATc               >  2:1000079/1‑150 (MQ=255)
                                                                                                                                       aaTGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGTTATCg              >  1:1680577/1‑150 (MQ=255)
                                                                                                                                       aaTGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCg              <  1:1125938/150‑1 (MQ=255)
                                                                                                                                        aTGGACGGCGATGATACATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGCTTGTTGGTATCGt             >  1:1620910/1‑150 (MQ=255)
                                                                                                                                         tttaaggCGATTTATCATCGCCGTCCTTTTTTCGACATACTAGATTCTTCTCGCATGCAAGATTTTTTTCTGCAAATAGACATTTTCAAGGGAGGAACTCATCACTCCCTTGCTGGTAGAGGATTGTATAAATTGTTTGTTTGTATCTTa            <  2:1148167/145‑1 (MQ=255)
                                                                                                                                          ggACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGATGGTAGAGGCGTGGATAAATTGGTTGTTGGTATAGTaa           >  2:1077290/1‑150 (MQ=255)
                                                                                                                                            aCGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAAt         <  1:209808/150‑1 (MQ=255)
                                                                                                                                            aCGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTAGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAAt         >  2:1520715/1‑150 (MQ=255)
                                                                                                                                               gCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAATAcc      >  2:1384910/1‑150 (MQ=255)
                                                                                                                                               gCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAATAca      >  1:614194/1‑149 (MQ=255)
                                                                                                                                                cGATGTCTCATCGCCGTCCATTTTTGGACATACTAGATTCGTCTCGCCTGCCTGAAATTTTTCTGGCAATAGACATTATCAAGGGAGGAACGGATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAATACCt     <  1:1239075/150‑1 (MQ=255)
                                                                                                                                                cGATGTATCATCGCCGTCCATTTTTCGACATACAAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAATACCt     <  1:473624/150‑1 (MQ=255)
                                                                                                                                                   tGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAATACCTACa  >  2:1276260/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                  
TACAGAAGGAGTAACTTTCATTTGTTCCATGTTAACCACTTTTTCAGGGGTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAATACCTACA  >  NC_000913/1792092‑1792387

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: