Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,830,061 G→T 8.8% A235A (GCC→GCA astA ← arginine succinyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,830,0610GT 8.8% 273.9 / 13.7 99A235A (GCC→GCAastAarginine succinyltransferase
Reads supporting (aligned to +/- strand):  ref base G (45/44);  new base T (5/4);  total (51/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.45e-01

Only 100 of 102 total aligned reads displayed.

CTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGCAGAGAAAATCGGCGCGGCTAAAATCCA  >  NC_000913/1829914‑1830209
                                                                                                                                                   |                                                                                                                                                    
cTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGTGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGc                                                                                                                                                    <  1:1705738/150‑1 (MQ=255)
cTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGc                                                                                                                                                    >  1:696575/1‑150 (MQ=255)
  gcgcAGGCTGCCCTTCTGCCACTTCCACCCGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGGCAAAGATGTCGATAGAGTTTCGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAg                                                                                                                                                  >  2:1202098/1‑150 (MQ=255)
     cAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGgcg                                                                                                                                               >  1:748300/1‑150 (MQ=255)
         acgCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCggg                                                                                                                                           >  2:1754129/3‑149 (MQ=255)
          ggCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCCTGCCCGCGCGGtg                                                                                                                                          <  1:1864856/149‑2 (MQ=255)
           gCCCTTCTGCCACTTCCACCCGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATCGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGttt                                                                                                                                         <  2:1380485/150‑1 (MQ=255)
           gCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGttt                                                                                                                                         <  1:248613/150‑1 (MQ=255)
            cccTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCCTGCCCGCGCGGtgct                                                                                                                                        <  2:32436/150‑4 (MQ=255)
             ccTTCTGCCACTTCCACCAGCCGACTTTTACGGATTGCGCGCACGAGGTCGATGTCACACTCAAGCGTCGGCCCACCGGCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCCGCACCGCGAGGGCAGGCGCGGTTTGc                                                                                                                                       <  2:1253201/150‑1 (MQ=255)
             ccTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCCCCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGc                                                                                                                                       >  2:466025/1‑150 (MQ=255)
              cTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCCCGCGGTCGCTGTCACACTCAAGCGTCGGCCCACCGTCGAAGATGTCGCTATAGTTACGGTAGCGAAAACGTGCTTTCTCCAGCACACCGCGGGCAGGCGCGGGTTGCg                                                                                                                                      >  1:1799409/1‑150 (MQ=255)
              cTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCCTGCCCGCGCGGtgctgg                                                                                                                                      <  2:1879688/150‑6 (MQ=255)
               ttCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCgg                                                                                                                                     <  1:1324828/150‑1 (MQ=255)
               ttCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCgg                                                                                                                                     <  2:1854648/150‑1 (MQ=255)
                tCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGa                                                                                                                                    <  2:737946/150‑1 (MQ=255)
                  tGCCACTTCCAGCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTAGGGCCACCTTCAAAGATGTCGATATAGTTAAGGTAGCGAATACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATg                                                                                                                                  <  2:123745/150‑1 (MQ=255)
                   gCCACTTCCCCCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGt                                                                                                                                 <  1:860062/150‑1 (MQ=255)
                   gCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCGCCGCGCGGGCAGGCGCGGTTTGCGGATGt                                                                                                                                 >  2:362338/1‑150 (MQ=255)
                   gCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGt                                                                                                                                 >  1:61871/1‑150 (MQ=255)
                    ccACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGAGCAGGCGCGGTTTGCGGATGTa                                                                                                                                >  2:1630812/1‑150 (MQ=255)
                      aCTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTAcc                                                                                                                              <  2:1741135/150‑1 (MQ=255)
                      aCTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGGCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTAcc                                                                                                                              <  2:1526032/150‑1 (MQ=255)
                         tCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACAGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTGTCTCCAGCACCGCGCGGGCAGGCGCGGGTTGCGGATGTACCTGa                                                                                                                           >  1:1540383/1‑150 (MQ=255)
                          ccaccaGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGAc                                                                                                                          <  2:65992/150‑1 (MQ=255)
                          ccaccaGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTGGCGGGTGTACCGGAc                                                                                                                          >  1:154599/1‑150 (MQ=255)
                                       tttACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTg                                                                                                             <  2:1116036/150‑1 (MQ=255)
                                        ttACGGATGGCGAGCAAGCGGTCGTTGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGTTGACGTCCTgg                                                                                                            <  2:1670751/150‑1 (MQ=255)
                                           cGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCATTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCt                                                                                                         >  2:1888646/1‑150 (MQ=255)
                                              aTGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGAGGTACCTGACCGATGACGTCCTGGGCTTcc                                                                                                      >  2:133380/1‑150 (MQ=255)
                                               tGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTTCCTGCCCGATGACGTCCTGGGCTTCCt                                                                                                     >  2:1033860/1‑150 (MQ=255)
                                                 gcgcgcACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTgg                                                                                                   <  2:1461256/150‑1 (MQ=255)
                                                      cACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGCCGTCCTGGGCTTCCTGGGATaa                                                                                              >  1:709126/1‑150 (MQ=255)
                                                          cgGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGAGGTACCTGACCGATGACGGCCTGGGCTTCCTGGGATTAAAAg                                                                                          >  1:245425/1‑150 (MQ=255)
                                                             tCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTgg                                                                                       <  2:863848/150‑1 (MQ=255)
                                                             gcGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTAGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTgg                                                                                       <  2:1460885/149‑1 (MQ=255)
                                                              cgctGTCACACTCAAGCGTCGGCCCACCGTCAAAGAAGTCGCTATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTggg                                                                                      <  2:754958/147‑1 (MQ=255)
                                                              cgctGTCACACGCAAGCGTCGGCCCACCGTCAAAGATGGCGATATAGGTTCGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGAGGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTggg                                                                                      <  1:791986/147‑1 (MQ=255)
                                                              cGATGTCACACTCAAGCGTCGGCCCACCTTCAAAGATGTCGATATAGATACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTggg                                                                                      >  2:1888749/1‑150 (MQ=255)
                                                              cGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTggg                                                                                      <  2:449534/150‑1 (MQ=255)
                                                               gaagTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGt                                                                                     <  1:1798681/147‑1 (MQ=255)
                                                               gATCTCACACTCAAGCGTCGGCCCACCGTCAAAGAAGTCGATATAGTAACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGt                                                                                     <  1:154045/150‑1 (MQ=255)
                                                                 tGTCACACTCATGCGTCGGCCCACCGTCAATGATGTCGATATAGTTAAGGTAGCGAAAACCTTCTTTCTCCAGCTCCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTTGGCTTCCTGGGATAAAAAGTGGGtat                                                                                   <  2:1204267/150‑1 (MQ=255)
                                                                  gTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGtata                                                                                  <  1:915615/150‑1 (MQ=255)
                                                                   tCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAACCCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGGTTGCGGATGTACCTGAACGATGCAGTCCTCGGCTTCCTGGGATAAAAAGGGGGTATAg                                                                                 >  2:361085/1‑150 (MQ=255)
                                                                   tCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATTAAAAGTGGGTATAg                                                                                 >  2:251738/1‑150 (MQ=255)
                                                                   tCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAg                                                                                 >  1:21859/1‑150 (MQ=255)
                                                                   tCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAg                                                                                 >  2:1721682/1‑150 (MQ=255)
                                                                        cTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCgg                                                                            >  1:438720/1‑150 (MQ=255)
                                                                        cTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCgg                                                                            <  1:235119/150‑1 (MQ=255)
                                                                        cTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCgg                                                                            <  2:713415/150‑1 (MQ=255)
                                                                         tCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGa                                                                           <  1:1355107/150‑1 (MQ=255)
                                                                          cAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTGTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGAt                                                                          >  1:978995/1‑150 (MQ=255)
                                                                            aGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGt                                                                        <  1:1490792/150‑1 (MQ=255)
                                                                               gTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTc                                                                     >  1:725612/1‑150 (MQ=255)
                                                                                 cGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCgg                                                                   <  1:4905/150‑1 (MQ=255)
                                                                                  ggCCCACCGTCAAAGAGGTCGATTTAGATACGGGAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCTGATGTACCTGACCAATGACGGCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGc                                                                  <  2:1047368/150‑1 (MQ=255)
                                                                                   gCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCa                                                                 <  1:1474559/150‑1 (MQ=255)
                                                                                    cccACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCAt                                                                >  1:1528046/1‑150 (MQ=255)
                                                                                     ccACCGTCATAGAGGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTTGGCTTCATGGGATAAAAAGTGGGTATATATCGGATGTTTCGGCATc                                                               <  1:322201/150‑1 (MQ=255)
                                                                                      cACCGTCAAAGAGGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCAACGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGTTATAGATCGGATGTATCGGCATCa                                                              >  2:1723487/1‑150 (MQ=255)
                                                                                           tCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCt                                                         >  1:1322615/1‑150 (MQ=255)
                                                                                             aaaGCTGTCGAATTAGTTACTGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAACGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGc                                                       <  2:1752031/150‑1 (MQ=255)
                                                                                             aaaGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGCTGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGc                                                       <  2:334528/150‑1 (MQ=255)
                                                                                             aaaGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGc                                                       <  2:475122/150‑1 (MQ=255)
                                                                                             aaaGATGTCGATATAGTGACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGGAGGCGCGGTTTGCGGATTTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAATAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGc                                                       <  2:681541/150‑1 (MQ=255)
                                                                                                          taGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCttt                                          <  1:71771/150‑1 (MQ=255)
                                                                                                            gTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTg                                        >  1:667963/1‑150 (MQ=255)
                                                                                                                 ggTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGAACGGATGTTTCGGCATCAGTTCTGCAATAAATGACTTTTGCCCgg                                   >  1:594678/1‑150 (MQ=255)
                                                                                                                     gCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCCATAAATGCCTTTTGCCCGGTGcc                               >  1:1563483/1‑150 (MQ=255)
                                                                                                                     gCGAAAACCTTCTTTCTCCAGCACCGCGAGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTTGGATAAAAAGTGGGTATAGATAGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCAGGTGcc                               >  1:591074/1‑150 (MQ=255)
                                                                                                                      cGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGcag                              >  1:1434423/1‑148 (MQ=255)
                                                                                                                      cGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCg                              >  1:1442471/1‑150 (MQ=255)
                                                                                                                       gAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAGAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGc                             >  1:407889/1‑150 (MQ=255)
                                                                                                                            ccTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGCAgaga                        >  1:1399775/1‑150 (MQ=255)
                                                                                                                               tctttctCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCGTGGGCTTCGTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGTCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGCAGAGaaaa                     <  1:1327673/150‑1 (MQ=255)
                                                                                                                               tctttctCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGCAGAGaaaa                     >  2:478522/1‑150 (MQ=255)
                                                                                                                               tctttctCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCAGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGCAGAGaaaa                     >  2:1668327/1‑150 (MQ=255)
                                                                                                                               tcatgctcCCGCCCCGGGCGTGCTTGTGCGGTTTTCGTTTTTAGCTGACGGTTGACGTCCTGGTCTTCGTGTGATAAAACGTGGGTATTGTTGGGATGTTTCGGCATCAGTTCTGCAATAACTGCCTTTTGCCCGGTGCCGCAGAGaaaa                     <  2:1040434/145‑1 (MQ=255)
                                                                                                                                  ttctCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGCAGAGAAAATCg                  >  1:168302/1‑150 (MQ=255)
                                                                                                                                   tctcCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGCAGAGAAAATCgg                 <  1:703442/150‑1 (MQ=255)
                                                                                                                                     tcCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGCAGAGAAAATCGgcg               >  2:23171/1‑150 (MQ=255)
                                                                                                                                     tcCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGCAGAGAAAATCGgcg               >  1:1653209/1‑150 (MQ=255)
                                                                                                                                     tcCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGCAGAGAAAATCGgcg               >  2:438562/1‑150 (MQ=255)
                                                                                                                                     ccgcaaaCCGCGCCTGCCCGCGCGGTTTGCGGATGTACCTGACCGCTGACGTCCTGGGCGTCCTGGGATAAAAAGGGGGTTTAGAGCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTGTTGCCCGGTGCCGCAGAGAAAATCGgcg               >  1:283132/7‑150 (MQ=255)
                                                                                                                                      cgcaaaCCGCGCCTGCCCGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGCAGAGAAAATCGgcgc              >  1:402921/6‑150 (MQ=255)
                                                                                                                                      ccAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGCAGAGAAAATCGgcgc              >  2:1084510/1‑150 (MQ=255)
                                                                                                                                      ccAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGCAGAGAAAATCGgcgc              >  1:692191/1‑150 (MQ=255)
                                                                                                                                       gcaaaCCGCGCCTGCCCGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGCAGAGAAAATCGGcgcg             >  2:1786093/5‑150 (MQ=255)
                                                                                                                                         gcgccGCGCGGGCAGGCGCGGGTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCAGGGATAAAAAGTGGGGATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGCAGAGAAAATCGGCGCGGc           <  1:362338/147‑1 (MQ=255)
                                                                                                                                         gCACCGCGCGGGCAGGCGCGGTTTGCGGATGTTCCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGAGGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGCAGAGATAATCGGCGCGGc           >  1:1679010/1‑150 (MQ=255)
                                                                                                                                         gCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGTCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGCAGAGAAAATCGGCGCGGc           <  1:1196441/150‑1 (MQ=255)
                                                                                                                                         gCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGGCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGCAGAGAAAATCGGCGCGGc           <  1:1557990/150‑1 (MQ=255)
                                                                                                                                         ccACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGCAGAGAAAATCGGCGCGGc           <  2:774332/149‑1 (MQ=255)
                                                                                                                                         aaaCCGCGCCTGCCCGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGCAGAGAAAATCGGCGCGGc           >  2:750126/3‑150 (MQ=255)
                                                                                                                                          aaCCGCGCCTGCCCGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGCAGAGAAAATCGGCGCGGCt          >  2:1841439/2‑150 (MQ=255)
                                                                                                                                             cgcgcgGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGACCGGTGCCGCAGAGAAAATCGGCGCGGCTaaa       >  2:477483/1‑150 (MQ=255)
                                                                                                                                                  gggCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATTAATGCCTTTTGCCCGGTGCCGCAGAGAAAATCGGCGCGGCTAAAATCCa  >  1:544033/1‑150 (MQ=255)
                                                                                                                                                  gggCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGCAGAGAAAATCGGCGCGGCTAAAATCCa  <  1:1022340/150‑1 (MQ=255)
                                                                                                                                                  gggCAGGCGCGGATTGCGGATGTACCTGACCGATGACGTCATGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAACTGCCTTTTGCCCGGTGCCGCAGAGAAAATCGGCGCGGCTAAAATCCa  <  2:301477/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CTGCGCAGGCTGCCCTTCTGCCACTTCCACCAGCCGACTTTTACGGATGGCGCGCACGCGGTCGATGTCACACTCAAGCGTCGGCCCACCGTCAAAGATGTCGATATAGTTACGGTAGCGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGCAGAGAAAATCGGCGCGGCTAAAATCCA  >  NC_000913/1829914‑1830209

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: