Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,865,682 G→C 10.9% intergenic (‑46/+44) yeaE ← / ← mipA aldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent/scaffolding protein for murein synthesizing machinery

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,865,6820GC10.9% 358.2 / 27.3 117intergenic (‑46/+44)yeaE/mipAaldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent/scaffolding protein for murein synthesizing machinery
Reads supporting (aligned to +/- strand):  ref base G (47/57);  new base C (6/7);  total (53/64)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01

Only 100 of 117 total aligned reads displayed.

TCTGTTTTGCGCTGACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACC  >  NC_000913/1865536‑1865830
                                                                                                                                                  |                                                                                                                                                    
tCTGTTTTGCGCTGACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACg                                                                                                                                                   <  1:1888858/150‑1 (MQ=255)
   gTTTTGCGCTGACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGccc                                                                                                                                                <  2:648090/150‑1 (MQ=255)
   gTTTTGCGCTGACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGccc                                                                                                                                                >  1:562989/1‑150 (MQ=255)
     tttGCGCTGACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTg                                                                                                                                              <  1:1255833/150‑1 (MQ=255)
         cgcTGACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGtttt                                                                                                                                          <  2:617978/150‑1 (MQ=255)
         cgcTGACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTCACGCCCTGtttt                                                                                                                                          <  1:1876470/150‑1 (MQ=255)
         cgcTGACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTCACGCCCTGtttt                                                                                                                                          <  1:1884579/150‑1 (MQ=255)
          gcTGACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGttttt                                                                                                                                         <  2:1513050/150‑1 (MQ=255)
          gcTGACTGGCATCTTCGCCCATAAACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGttttt                                                                                                                                         <  1:37299/150‑1 (MQ=255)
           cTGACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTa                                                                                                                                        <  1:1044111/150‑1 (MQ=255)
           cTGACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTa                                                                                                                                        >  2:1673994/1‑150 (MQ=255)
            tGACTGGCATCTTCGCCCATATTCCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCAGCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTAt                                                                                                                                       <  1:161805/150‑1 (MQ=255)
            tGACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCCTTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTCACGCCCTGttttgca                                                                                                                                       <  1:969796/150‑4 (MQ=255)
             gACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATg                                                                                                                                      <  2:723513/150‑1 (MQ=255)
             gACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTCACGCCCTGttttgcat                                                                                                                                      <  1:527882/150‑5 (MQ=255)
             gACTGGCATCTTCGCACATATACTATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTCACGCCCTGttttgaat                                                                                                                                      <  1:1190024/150‑5 (MQ=255)
              aCTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGc                                                                                                                                     <  1:1666763/150‑1 (MQ=255)
                 ggCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGcaca                                                                                                                                  <  1:436538/150‑1 (MQ=255)
                   cATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACaaa                                                                                                                                >  1:425193/1‑150 (MQ=255)
                    aTCTTCGCCCATATACCATGATCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTAGATCCATTGTTTCCTCCCAGGTAATGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACaaaa                                                                                                                               <  1:187849/150‑1 (MQ=255)
                     tCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACAAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCCCCGTAATGCAAAACAGGGCGTGACGCCCTGGTTTTATGGACAAAAt                                                                                                                              >  2:587027/1‑150 (MQ=255)
                        tCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGcc                                                                                                                           <  2:1461268/150‑1 (MQ=255)
                        tCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGcc                                                                                                                           <  1:1438644/150‑1 (MQ=255)
                          gCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCAACGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCt                                                                                                                         >  1:822683/1‑150 (MQ=255)
                                ataCCATGTTCCCTGCCCTACGGCTGGCAGTTAGACATCGCCACAAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAg                                                                                                                   <  2:1740007/150‑1 (MQ=255)
                                 taCCATCTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTATTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGa                                                                                                                  <  2:410727/150‑1 (MQ=255)
                                  aCCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGAt                                                                                                                 >  2:887549/1‑150 (MQ=255)
                                   ccATGTTCCCTGCCCTCCGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATg                                                                                                                <  2:1408312/150‑1 (MQ=255)
                                   ccATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATg                                                                                                                >  2:1571591/1‑150 (MQ=255)
                                   ccATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATg                                                                                                                <  2:143144/150‑1 (MQ=255)
                                   ccATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATg                                                                                                                <  2:343875/150‑1 (MQ=255)
                                      tGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACAGTAATGCAAAACAGGGCGTGCCGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCAt                                                                                                             >  2:1515939/1‑150 (MQ=255)
                                       gTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCAtt                                                                                                            >  2:523435/1‑150 (MQ=255)
                                       cttCCCTGCCCTACGGCTGGCAGTGAGCCATCGCCACTAAATTGAATCATTTTTTGATTCATTGTATCCTCCCAGGTATTCAACCACCGTAATGCAAAACAGGGCGTGACGCCCCGATTTTATGCACAAAATGCCCTGGAAAGATGCAtt                                                                                                            >  2:1405463/2‑150 (MQ=255)
                                         tCCCTGCCCTACGGCTGGCAGTGAGACATCGCCGCTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTAGTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACATAATGCCCTGGAAAGATGCAGTAt                                                                                                          >  1:293496/1‑150 (MQ=255)
                                            cTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAg                                                                                                       <  2:544027/150‑1 (MQ=255)
                                             tGCCCTTCGGCTGGCAGTGTGACTTCGCAAATAAATTGAATAATTTTTGGTTGCATTGTTTCATCCCAGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGaa                                                                                                     <  2:1282295/150‑1 (MQ=255)
                                               cccTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTGGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTTTCAGAAt                                                                                                    >  1:314176/1‑150 (MQ=255)
                                                ccTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGGATCCTCCCACGTATTGCACCACCGTAAAGCAAAACAGGGCGTGACGCCCTGTTTTTATGCTCAAAATGCCCGTGACTGCTGAATTATCAGaagt                                                                                                   >  2:317029/1‑148 (MQ=255)
                                                  tACTGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTTCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGTACAAAATGCCCTGGAAAGATGCACTATCAGAATTTg                                                                                                 <  1:297724/150‑1 (MQ=255)
                                                   aCGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCTTTGTTTCCTCCCAGGTATTGCACGACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGt                                                                                                <  2:1330768/150‑1 (MQ=255)
                                                       cTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGt                                                                                            <  1:150001/150‑1 (MQ=255)
                                                       cTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGt                                                                                            >  1:52764/1‑150 (MQ=255)
                                                       cTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGt                                                                                            >  2:248/1‑150 (MQ=255)
                                                        tGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTg                                                                                           <  2:37394/150‑1 (MQ=255)
                                                             gTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATccc                                                                                      >  2:588150/1‑150 (MQ=255)
                                                                agaCATCGACACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGt                                                                                   <  2:368303/150‑1 (MQ=255)
                                                                   cATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGa                                                                                <  1:266170/150‑1 (MQ=255)
                                                                    aTCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGaa                                                                               >  1:1260275/1‑150 (MQ=255)
                                                                    aTCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGaa                                                                               <  2:1395705/150‑1 (MQ=255)
                                                                     tagCCACTAAATTTAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTTTTGCACCACCGTAATGCAATACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGaaa                                                                              <  1:937645/148‑1 (MQ=255)
                                                                        ccACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCa                                                                           <  1:1329675/150‑1 (MQ=255)
                                                                            tAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGcc                                                                       <  2:1300202/150‑1 (MQ=255)
                                                                            tAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTCCACCACCGTAATGCAAAACAGGGCGTGACGCCCTTTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATAAGAATTTGTAGGTGATCCCGGTAGAAATCAGGcc                                                                       <  2:1585551/150‑1 (MQ=255)
                                                                            tAAATTGAATCATTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCa                                                                      <  1:133892/150‑1 (MQ=255)
                                                                             aaaTTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCa                                                                      >  2:1818874/1‑150 (MQ=255)
                                                                             aaaTTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGACCTGGAAAGATGCATTATCAGAATTAGTAGGTGAACCCGGTAGAAATCAGGCCa                                                                      >  2:1421589/1‑150 (MQ=255)
                                                                              aaTTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAg                                                                     >  1:72386/1‑150 (MQ=255)
                                                                                  gAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCa                                                                 <  1:1167396/150‑1 (MQ=255)
                                                                                   aaTCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCAGCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGACCTGGAAAGATGCATTATCAGAATTTGTAGGTGCTCCCGGTAGAAATCAGgccaggccag                                                                >  2:1333422/1‑150 (MQ=255)
                                                                                    aTCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAgg                                                               <  1:659230/150‑1 (MQ=255)
                                                                                     tCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGa                                                              >  1:446454/1‑150 (MQ=255)
                                                                                      ttttttttGTTGAATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAATACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAATTATGCATTATCAGAATATGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGAt                                                             <  2:592348/148‑1 (MQ=255)
                                                                                      cATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGAt                                                             >  1:178921/1‑150 (MQ=255)
                                                                                      cATTTTTTGGTGCATTGTTTCCTCCCAGGTATTGCACCACCGTATTGAAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCATGGAAATATGCATTATCAGAATTTGTATGTGATCCCGGTAGAATTCAGGCCAGTCCATGAt                                                             <  2:1893414/150‑1 (MQ=255)
                                                                                       aTTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGttt                                                            >  1:1520026/1‑150 (MQ=255)
                                                                                       aTTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGttt                                                            >  1:1512352/1‑150 (MQ=255)
                                                                                       aTTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGAtt                                                            >  1:1565794/1‑150 (MQ=255)
                                                                                       aTTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGAtt                                                            >  1:1475434/1‑150 (MQ=255)
                                                                                       aTTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGAtt                                                            <  1:580948/150‑1 (MQ=255)
                                                                                       aTTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAAGTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGAtt                                                            >  1:1157435/1‑150 (MQ=255)
                                                                                          ttttGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTAt                                                         <  2:425193/150‑1 (MQ=255)
                                                                                           tttGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCTGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATc                                                        <  2:1212824/150‑1 (MQ=255)
                                                                                             tgttgCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATcca                                                      <  1:1751778/150‑1 (MQ=255)
                                                                                              gttgCATTGTTTCCTCCCAGGTATTGCACCACCGTATTGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGCTGCATAATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATccac                                                     >  2:862573/1‑150 (MQ=255)
                                                                                              gttgCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATccac                                                     >  1:474279/1‑150 (MQ=255)
                                                                                              gttgCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCAAGGATTTATccac                                                     >  1:1878711/1‑150 (MQ=255)
                                                                                              gttgCATTGTTTCCACCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATccac                                                     <  2:734078/150‑1 (MQ=255)
                                                                                                  cATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATc                                                 >  1:193835/1‑150 (MQ=255)
                                                                                                    ttGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCgg                                               >  1:352000/1‑150 (MQ=255)
                                                                                                    ttGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCgg                                               >  1:1515423/1‑150 (MQ=255)
                                                                                                     tGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCggg                                              <  1:1539587/150‑1 (MQ=255)
                                                                                                      gTTTGCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTTGAAAGATGCATTATCAGAATTTGTATGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGc                                             <  2:490362/150‑1 (MQ=255)
                                                                                                      gTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGc                                             >  2:342689/1‑150 (MQ=255)
                                                                                                        ttCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTg                                           <  1:434277/150‑1 (MQ=255)
                                                                                                          cctccCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGTTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTc                                         >  2:1647726/1‑150 (MQ=255)
                                                                                                          cctccCAGGTATTGAACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAACTGCCCTGGAAAGATGCAGGATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCACGCCAGGAGTGATCCACCATCGGGCTggc                                         >  1:303670/1‑148 (MQ=255)
                                                                                                           ctccCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCa                                        >  2:199851/1‑150 (MQ=255)
                                                                                                                 ggTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTGCAGGATTTATCCACCATCGGGCTGTCAGTAACt                                  >  1:1003560/1‑150 (MQ=255)
                                                                                                                 ggTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACt                                  <  2:590011/150‑1 (MQ=255)
                                                                                                                        caccacCGTAATGAAAAAATGGGCGTGACGCCCTGTTTTTATGCAAAAAATCCCCTGTAACTATGATTAATCAGAATTTGTCGGTGATCCCGGTATAAATCAGGCCCGCCCAGGATTTACCCACCATCGGGCTGTCAGTAACTtcatccg                           <  2:1311584/150‑3 (MQ=255)
                                                                                                                          ccaccGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATcagac                         <  1:1673994/150‑1 (MQ=255)
                                                                                                                                tgcataaaaaCAGGGCGTCACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTAACAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCTGGCTGTCAGTAACTACATCAGCCAGACgg                   >  2:1472704/7‑150 (MQ=255)
                                                                                                                                 gcataaaaaCAGGGCGTCACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACggg                  >  1:1003966/6‑150 (MQ=255)
                                                                                                                                  cataaaaaCAGGGCGTCACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGgt                 >  1:942753/5‑150 (MQ=255)
                                                                                                                                   tGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGgtg                <  2:710239/150‑1 (MQ=255)
                                                                                                                                     cAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTa              <  2:474279/150‑1 (MQ=255)
                                                                                                                                            gggCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTACCTTCATCAGACAGACGGGTGTAGCGTGCg       >  1:1261168/1‑150 (MQ=255)
                                                                                                                                             ggCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCgg      >  1:792665/1‑150 (MQ=255)
                                                                                                                                                 tGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTAcc  >  2:1473439/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
TCTGTTTTGCGCTGACTGGCATCTTCGCCCATATACCATGTTCCCTGCCCTACGGCTGGCAGTGAGACATCGCCACTAAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACC  >  NC_000913/1865536‑1865830

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: