BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,928,799	A→C	8.1%	L14R (CTT→CGT)	ptrB ←	protease II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,928,799	0	A	C	8.1%	327.9 / 16.1	120	L14R (CTT→CGT)	ptrB	protease II
Reads supporting (aligned to +/- strand): ref base A (51/58); new base C (4/6); total (55/65)
Fisher's exact test for biased strand distribution p-value = 7.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.64e-01

Only 100 of 121 total aligned reads displayed.

CCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGGCGCG  >  NC_000913/1928650‑1928945

ccTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGaa                                                                                                                                                    <  2:822655/150‑1 (MQ=255)

  tttAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGc                                                                                                                                                  <  2:1103845/150‑1 (MQ=255)

  tttAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGc                                                                                                                                                  <  2:1775704/150‑1 (MQ=255)

    tAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGACCGCGTATCGTCCCTCAGCCAGTCGTAATTATCGATGCGCGTATCGCCATGAAGCGt                                                                                                                                                >  1:323667/1‑150 (MQ=255)

    tAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGAAGACCGTAACTATTTACTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCAGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCAACAAGCGt                                                                                                                                                >  1:1146365/1‑150 (MQ=255)

         tGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATgg                                                                                                                                           <  1:887245/150‑1 (MQ=255)

          gCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGACGCTTCATGGc                                                                                                                                          <  1:387835/150‑1 (MQ=255)

          gCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGACGCTTCATGGc                                                                                                                                          <  1:1497719/150‑1 (MQ=255)

           cGATCCAGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCTTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTGCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCg                                                                                                                                         <  1:365667/150‑1 (MQ=255)

             aTCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGACGCTTCATGGCgat                                                                                                                                       <  1:1783965/150‑3 (MQ=255)

              tCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGGAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGACGCTTCATGGCgata                                                                                                                                      <  1:370824/150‑4 (MQ=255)

               ccTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGACGCTTCATGGCgatac                                                                                                                                     <  1:489485/150‑5 (MQ=255)

                cTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTgggg                                                                                                                                    <  1:1695749/150‑1 (MQ=255)

                 tGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGa                                                                                                                                   >  1:1456384/1‑150 (MQ=255)

                  gCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGaa                                                                                                                                  >  2:1622332/1‑150 (MQ=255)

                        cTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATgcggg                                                                                                                            <  2:1620286/150‑1 (MQ=255)

                        cTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATgcggg                                                                                                                            >  1:436895/1‑150 (MQ=255)

                          tgttgtGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTTTCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATgcgggcg                                                                                                                          <  1:173418/150‑1 (MQ=255)

                          tgttgtGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATgcgggcg                                                                                                                          <  1:1452195/150‑1 (MQ=255)

                               tgAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGAGCGGCttt                                                                                                                     >  2:1526181/1‑150 (MQ=255)

                                gAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTTTCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCAGGCGGCtttt                                                                                                                    >  2:301440/1‑150 (MQ=255)

                                  ggCCATCACCCGATGACCGTAACTATTTTCTTGTTGCATGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGACAGTAGTAATTATCGATGCTCTTATCTCCATGAAGCGTCATGGCTTGGGGAATGCGGGCGGCTTTTgg                                                                                                                  >  1:288413/1‑150 (MQ=255)

                                  ggCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTgg                                                                                                                  >  1:683020/1‑150 (MQ=255)

                                  ggCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGAGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGATAGCGGGCGGCTTTTgg                                                                                                                  >  1:424653/1‑150 (MQ=255)

                                   gCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGt                                                                                                                 >  1:601375/1‑150 (MQ=255)

                                    ccATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTa                                                                                                                >  2:1635125/1‑150 (MQ=255)

                                     cATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAg                                                                                                               <  2:1818033/150‑1 (MQ=255)

                                       tCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCa                                                                                                             <  1:483471/150‑1 (MQ=255)

                                            cGATGACCGTAACTATTTTCTTGGTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTa                                                                                                        <  2:1745261/150‑1 (MQ=255)

                                             gATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTAt                                                                                                       <  2:1024766/150‑1 (MQ=255)

                                                gACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGt                                                                                                    <  1:845566/150‑1 (MQ=255)

                                                        cTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTg                                                                                            >  1:1405880/1‑150 (MQ=255)

                                                         tATTTTCTTGTTGCAGGTAGTCCAGGACTTCTTGCTCAGAACGCGTATCGTCACGAAGCCAGTTGTAATTATCGATGCGCGTATCGCCATGAAGCGTCAGGGCGTCGGGTATCCGTGCGGCTTTTGGTAGCATGTTATTGTGCTTTCTgg                                                                                           >  1:105010/1‑150 (MQ=255)

                                                         tATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTgg                                                                                           <  1:1301487/150‑1 (MQ=255)

                                                         tATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTgg                                                                                           <  1:405324/150‑1 (MQ=255)

                                                          aTTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGt                                                                                          <  2:1833525/150‑1 (MQ=255)

                                                           ttttCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACTCGTATCGTCCCGCAGCCAGTAGGAATTATCGATGCGCGTATCGCCATGAACCGTCATGGCGTGGGTAATGCGGGCGGCATTTGGTAGCATGTTATTGTTCTTTCTGGtt                                                                                         >  2:1427204/1‑150 (MQ=255)

                                                             ttCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGa                                                                                       <  2:1713146/150‑1 (MQ=255)

                                                                ttgttgCAGGTAGTCCAGGACTTCTGGCTGTGAACGCGTATCGTCCCTCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAAc                                                                                    <  1:1732885/150‑1 (MQ=255)

                                                                 tgttgCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACa                                                                                   >  1:142974/1‑150 (MQ=255)

                                                                    tgCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCt                                                                                >  1:194659/1‑150 (MQ=255)

                                                                    tgCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCt                                                                                >  1:1905713/1‑150 (MQ=255)

                                                                     gCAGGTAGTCCAGGACTTCTGGCTGATAACGCGTATCGTCCCGCTGCCTGTAGTAATTATCGATGCGCGTATCGCCATTAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCtt                                                                               <  1:1732008/150‑1 (MQ=255)

                                                                        ggTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTtata                                                                            >  2:1064592/1‑150 (MQ=255)

                                                                        ggTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTtata                                                                            >  2:1476801/1‑150 (MQ=255)

                                                                            gTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAggg                                                                        >  1:165985/1‑150 (MQ=255)

                                                                             tCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCTGTAGTAATTTTCGATGCGCGTATCGCCAGGAAGCGTCATGGCGGGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTAGAAgggg                                                                       >  1:1724540/1‑149 (MQ=255)

                                                                              ccAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTg                                                                      >  2:1682004/1‑150 (MQ=255)

                                                                              ccAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTg                                                                      <  2:194659/150‑1 (MQ=255)

                                                                              ccAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTg                                                                      <  2:763601/150‑1 (MQ=255)

                                                                              ccAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTg                                                                      <  1:1046429/150‑1 (MQ=255)

                                                                              ccAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTg                                                                      <  1:1022080/150‑1 (MQ=255)

                                                                              ccAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTg                                                                      <  1:1574206/150‑1 (MQ=255)

                                                                               cAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTgg                                                                     <  2:364039/150‑1 (MQ=255)

                                                                               cAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTgg                                                                     >  1:30115/1‑150 (MQ=255)

                                                                                aGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGc                                                                    <  1:440614/150‑1 (MQ=255)

                                                                                aGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGc                                                                    <  1:1471458/150‑1 (MQ=255)

                                                                                   aCTTCTGGCTGAGAACGCGTATCGGCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCaaa                                                                 <  2:729090/150‑1 (MQ=255)

                                                                                     ttCTGGCTGACAACGCGGAAGGTCCCCCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGATGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAAc                                                               <  2:316561/150‑1 (MQ=255)

                                                                                      tCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACt                                                              >  2:269798/1‑150 (MQ=255)

                                                                                       cTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTc                                                             >  1:1367004/1‑150 (MQ=255)

                                                                                        tGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCa                                                            >  2:1682063/1‑150 (MQ=255)

                                                                                         ggCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTGTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCAc                                                           >  2:1813883/1‑150 (MQ=255)

                                                                                         ggCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATATGGGTGGCAAAACTCAc                                                           >  2:246004/1‑150 (MQ=255)

                                                                                             gagaACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCggg                                                       <  2:1830368/150‑1 (MQ=255)

                                                                                              agaACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCgggg                                                      >  1:655340/1‑150 (MQ=255)

                                                                                              agaACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCgggg                                                      <  1:1756465/150‑1 (MQ=255)

                                                                                               gaACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGa                                                     >  1:803702/1‑150 (MQ=255)

                                                                                                  cgcgTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGc                                                  >  1:1690801/1‑150 (MQ=255)

                                                                                                  cgcgTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGc                                                  >  1:1340332/1‑150 (MQ=255)

                                                                                                   gcgTATCGTCCCGCAGCCAGTAGTAAATATCGAGGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCCGGCGGCGTTTGGTAGCATGTGAGTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCa                                                 <  1:301440/150‑1 (MQ=255)

                                                                                                    cgTATCGTCACGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATCAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTATGGTAGCATGTTATTTTTCTTTCTGGTTGAAACATCTTATAAGGGTGTCAAAACTAACCGGGGATGCaa                                                >  2:469332/1‑150 (MQ=255)

                                                                                                       agcGTCCCGCAGCCAGTAGTAATAATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGTGAATGCGGGCGGCTTTTGGTAGCATGTTATTTTTCTTTCTGGTTGAAACATCTTATACGGGTGGCAAAACTCACCGGGGATGCAAGCg                                             <  1:128900/148‑1 (MQ=255)

                                                                                                       aTCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCg                                             <  2:311818/150‑1 (MQ=255)

                                                                                                           tCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAAc                                         <  1:538467/150‑1 (MQ=255)

                                                                                                            cccGCATCCAGTAGTAAATCTCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTATCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACa                                        <  1:70697/150‑1 (MQ=255)

                                                                                                            cccGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGCAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTCGATGAAACATCTTATACGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACa                                        <  1:1171562/150‑1 (MQ=255)

                                                                                                            cccGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACa                                        >  1:847887/1‑150 (MQ=255)

                                                                                                                cAGCCAGTAGTAATTCTCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGCGAATGCGGGCGGCTTTTGGTAGCTTGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAgggg                                    <  2:333439/150‑1 (MQ=255)

                                                                                                                cAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATTGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAgggg                                    <  1:677361/150‑1 (MQ=255)

                                                                                                                  gCCAGTAGTAATTATCGATGCGCGTATCTCGATGTAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAg                                  <  1:744720/150‑1 (MQ=255)

                                                                                                                  gCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAg                                  <  1:1488505/150‑1 (MQ=255)

                                                                                                                    cAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTc                                >  1:335257/1‑150 (MQ=255)

                                                                                                                        agtaATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTg                            <  1:442961/150‑1 (MQ=255)

                                                                                                                         gtaATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGc                           >  1:699124/1‑150 (MQ=255)

                                                                                                                            aTTATCGATGCGCGTATCGCCATGAAGCGTCTTGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTa                        <  2:1897015/150‑1 (MQ=255)

                                                                                                                                tCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAgatg                    <  2:1522569/150‑1 (MQ=255)

                                                                                                                                   aTGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGatgatg                 <  2:114367/150‑1 (MQ=255)

                                                                                                                                    tGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGAtgatga                <  2:1405880/150‑1 (MQ=255)

                                                                                                                                      cgcgTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACa              >  1:1536191/1‑150 (MQ=255)

                                                                                                                                        cgTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCGGGTTGCAACAGCTTATAAGGGTGGCAACACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCGTAGATGATGACAgg            >  2:1776136/1‑150 (MQ=255)

                                                                                                                                          tATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTa          >  1:1867131/1‑150 (MQ=255)

                                                                                                                                          ccacGCCATGACGCTTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTa          >  1:258573/4‑150 (MQ=255)

                                                                                                                                            tCGCCATGAAGCGTCATGGCGTGTGGAATGCGGGCGGCTTTTGGTAGCATTTTTTTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGAAAAACTCACCGGGGTTGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAAt        <  1:1897862/150‑1 (MQ=255)

                                                                                                                                            tCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCTAGCGAAACAGGGGAGTCTTTGCTTAGATGATGACAGGTAAt        <  1:1064592/150‑1 (MQ=255)

                                                                                                                                            tCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAAt        >  2:1828653/1‑150 (MQ=255)

                                                                                                                                            tCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAAt        <  1:1476801/150‑1 (MQ=255)

                                                                                                                                            acGCCATGACGCTTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAAt        >  2:708520/2‑150 (MQ=255)

                                                                                                                                              gCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGGATGTTATTGTTCTTGCTGGTTGAAACATCTTATTAGGGTGGCAAAACTCACCGGGGATGAAAGCGAAACAGGGGAGTCATTGCTTAGATGTTGACAGGTATTgg      >  1:97230/1‑150 (MQ=255)

                                                                                                                                                  tGACGCTTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGGcgcg  >  1:1327059/1‑150 (MQ=255)

CCTTTAAGATGCGATCCTGCAAGGCTTGTTGTGAGGCCATCACCCGATGACCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTTTCTGGTTGAAACATCTTATAAGGGTGGCAAAACTCACCGGGGATGCAAGCGAAACAGGGGAGTCATTGCTTAGATGATGACAGGTAATGGCGCG  >  NC_000913/1928650‑1928945

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`