Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,074,687 T→G 8.0% intergenic (+29/‑92) flu → / → yeeR CP4‑44 prophage; antigen 43 (Ag43) phase‑variable biofilm formation autotransporter/CP4‑44 prophage; putative membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,074,6870TG 8.0% 246.3 / 2.1 120intergenic (+29/‑92)flu/yeeRCP4‑44 prophage; antigen 43 (Ag43) phase‑variable biofilm formation autotransporter/CP4‑44 prophage; putative membrane protein
Reads supporting (aligned to +/- strand):  ref base T (54/52);  new base G (7/5);  total (61/59)
Fisher's exact test for biased strand distribution p-value = 7.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 127 total aligned reads displayed.

ACTGGAAGCCCGTGTCCGGGAAAATATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATCGCAGGATTTGCCATTCTTCGCC  >  NC_000913/2074538‑2074836
                                                                                                                                                     |                                                                                                                                                     
aCTGGAAGCCCGTGTCCGGGAAAATATCACCCTGGGCGTTCATGCCGGTTATGCACACAGAGTCAGCGTCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTTGTCCCGGt                                                                                                                                                       <  1:1852408/150‑1 (MQ=255)
aCTGGAAGCCCGTGTCCGGGAAAATATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGt                                                                                                                                                       <  1:1545824/150‑1 (MQ=255)
aCTGGAAGCCCGTGTCCGGGAAAATATCACCCTGGGCGTGCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGt                                                                                                                                                       <  1:708110/150‑1 (MQ=255)
 cTGGAAGCCCGTGTCCGGGAAAATATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGtc                                                                                                                                                      >  1:43819/1‑150 (MQ=255)
    gAAGCCCGTGTCCGGGAAAATATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGAAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGgcatc                                                                                                                                                   >  1:946187/1‑150 (MQ=255)
         ccGTGTCCGGGAAAATATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGCgcatcaaggc                                                                                                                                              >  1:1580781/1‑146 (MQ=255)
            tgtCCGGGAAAATATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTTAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCgg                                                                                                                                           <  1:495163/150‑1 (MQ=255)
            tgtCCGGGAAAATATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCgg                                                                                                                                           <  1:1736988/150‑1 (MQ=255)
              tCCGGGTAAATATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGa                                                                                                                                         <  1:581706/150‑1 (MQ=255)
               ccGGGAAAATATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGAc                                                                                                                                        <  2:1869178/150‑1 (MQ=255)
               ccGGGAAAATATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGGCATGATGACCGGGAc                                                                                                                                        <  2:262812/150‑1 (MQ=255)
                cGGGAAAATATCACCCTGGGCGTGCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGAcc                                                                                                                                       <  2:1240651/150‑1 (MQ=255)
                 gggAAAATATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGGCAACATGCCCGGGcccc                                                                                                                                      >  1:1761366/1‑150 (MQ=255)
                  ggAAAATATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCg                                                                                                                                     >  2:443001/1‑150 (MQ=255)
                       atatCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCg                                                                                                                                >  1:1763422/1‑150 (MQ=255)
                        tatCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATACTGACCGGGACCCCGACCgg                                                                                                                               >  1:1647743/1‑150 (MQ=255)
                            aCCCTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGTCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCa                                                                                                                           >  2:1186338/1‑150 (MQ=255)
                            aCCCTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCGGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCa                                                                                                                           >  2:1060736/1‑150 (MQ=255)
                               cgggggGTTCCGGCGGTTTATGCCCACAGCGTCCGCGGCAGCAGCGCTGACGGGTAAAAGGGTCCGCCCACACTTAATGTGACCTTCTGACACAACCATCGCCTCTCTGTGGCCCCGGTCATCATGCCGGGGACCCGGACCGGCGCAACg                                                                                                                        <  2:709059/148‑1 (MQ=255)
                                tGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCCCACTGAATGTGCCCTTCTGACAGCACCATCGCCTCTCTGTGGTCCCGGTCATCAAGACCGGGACCCCGCCCGGCGCAAccg                                                                                                                       >  1:750874/1‑148 (MQ=255)
                                    cGTTCCGGCCGGTTATGCCCACAGCGTCAGCGGAAGCAGCGCTGATGGGTATAACGGTCAGGCCACACTGAATGAGACCTTCTGACATAACCATCGCCTCTCTGCGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCt                                                                                                                   <  2:904847/150‑1 (MQ=255)
                                    cGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCt                                                                                                                   >  1:1302681/1‑150 (MQ=255)
                                        cAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTAATAATGACCGGGGCCCGGACCGGGGCAACGGGTCGTCac                                                                                                               >  1:1566753/1‑149 (MQ=255)
                                          ggCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACg                                                                                                             >  1:48860/1‑150 (MQ=255)
                                            cggtttATGCCCACAGCGTCAGCGGCAGCTGCGCTGATGGTTATAAGGGTCAGGCCACAGTTAATGTGACCTTCTGACACACCCATCGCCTCTCTGGGGTCCCGTTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGcc                                                                                                           <  2:633350/146‑1 (MQ=255)
                                            ccGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCGTCAACGcc                                                                                                           >  2:1582130/1‑150 (MQ=255)
                                                 tATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGCCCGGCGCAACGGATCTTCAACGCCACAtt                                                                                                      >  1:25178/1‑150 (MQ=255)
                                                   tGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGCCCCGGACCGGCGCAACGGATCTTCAACGCCACATTCg                                                                                                    >  2:1310836/1‑150 (MQ=255)
                                                   tGCCCACAGCGTCAGCGGCAGCAGCGCGGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTCTGGTCCCGGTCATCATGACCGGGCCCCGGACCGGCGCAACGGATCTTCAACGCCAGATTCg                                                                                                    >  1:1264218/1‑150 (MQ=255)
                                                    gCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCCCATTCGc                                                                                                   >  2:1822547/1‑150 (MQ=255)
                                                     cccACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCt                                                                                                  <  1:1066289/150‑1 (MQ=255)
                                                     cccACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGGGAAAAATACCCGGACCCCGCCCCGCCCCACCGATATTAAACCCcacaataacg                                                                                                  >  1:404713/1‑144 (MQ=255)
                                                      ccACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTg                                                                                                 <  1:1080105/150‑1 (MQ=255)
                                                        acaGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGc                                                                                               <  1:1873823/150‑1 (MQ=255)
                                                        acaGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGc                                                                                               <  1:1362441/150‑1 (MQ=255)
                                                        acaGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCCGGCATCATGCCCGGGACCCCGACCGGCGCAACGGATCATCAACCCCCCCTTCCCTGGc                                                                                               >  2:201234/1‑150 (MQ=255)
                                                         caGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGATTGTGACCTTCTGACAGACCCATCGCCGCTCTGTGGGCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCa                                                                                              <  2:407214/150‑1 (MQ=255)
                                                          aGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCAt                                                                                             <  2:70580/150‑1 (MQ=255)
                                                              gcAGCGGCAGCAGCGCTGAAGGTTAAAACGGTCTGGCCACACTGACTGTGACATTCTTACAGAACCATCGCCTCTCTGTGGTCCCGGTCTTCATGACCGGGACCCGGACCGGAGCAACGGATCTTCAACGCCACATTCGCTGGCATTAAc                                                                                         <  1:1145137/149‑1 (MQ=255)
                                                               cAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGGCAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACa                                                                                        >  2:1801724/1‑150 (MQ=255)
                                                                aGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGGCATCATGCCCGGGACCCGGCCCGGCGCCACGGCTCTTCACCGCCCCATTCGCTGGCATTAACaa                                                                                       >  1:552518/1‑150 (MQ=255)
                                                                  cGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCCGTCATAATGACCGGGCACCGGACCGGCCCCAAGGTTATTCAACCCCACAATCGCGTGCATTAaaaaaa                                                                                     >  2:1611010/1‑148 (MQ=255)
                                                                       gcagcGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATg                                                                                >  1:1016685/1‑150 (MQ=255)
                                                                        cagcGCTGAACGGTATAACGGTCGGGCCACACTGAATGTTACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGTTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGa                                                                               <  1:101755/150‑1 (MQ=255)
                                                                               gAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATtcat                                                                        <  2:1251581/150‑1 (MQ=255)
                                                                                aaGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGCTCTTAAACGCCACATTAGCTGGCATTAACAATAAAATGATATtcata                                                                       >  2:962344/1‑149 (MQ=255)
                                                                                 aGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGCCCCGGACCGGCGCAACGGATCTTCAACGCCCCATTCGCTGGCATTAACAATAACATGATATtcatca                                                                      >  2:158944/1‑150 (MQ=255)
                                                                                  gggTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCCCATTCGCTGGCATTAACAATAACATGATATTAATAAc                                                                     >  2:1518632/1‑150 (MQ=255)
                                                                                    gTATAACGGTCTGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACgg                                                                   <  2:569189/150‑1 (MQ=255)
                                                                                    gTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGGCCCGGCCCGGCGCCACGGATCTTCAACGCCCCATTCGCGGGCATTAACAATAACATGATATTCATCACgg                                                                   >  1:1891440/1‑150 (MQ=255)
                                                                                      ataACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGGTGGCCTTAACAATAACATGATATTCATCACGGAg                                                                 >  1:978100/1‑150 (MQ=255)
                                                                                       taACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGt                                                                >  2:1660244/1‑150 (MQ=255)
                                                                                              cAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATg                                                         <  2:545274/150‑1 (MQ=255)
                                                                                               aGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGGCAGAATGGCCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACCTTCGCGGGCATGAACAATAACAAGATATTCAGCACGGCGTGCCTATGt                                                        >  1:355950/1‑150 (MQ=255)
                                                                                                ggCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACGGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGtt                                                       <  2:1742729/150‑1 (MQ=255)
                                                                                                 gCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGCCCGGGCCCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAAAAACATGATATTCATCACGGAGTGACTATGTTa                                                      >  1:424376/1‑150 (MQ=255)
                                                                                                  caacacTTAATGGTACGTTCTGAAATAACCATCTCATCTCTGTGGTCCCGGTCTTAATGACCGGTACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTAc                                                     <  2:634668/148‑1 (MQ=255)
                                                                                                   cacaCTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACa                                                    <  1:276619/150‑1 (MQ=255)
                                                                                                    acacTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATTACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTAGCAGGCATTAACAATAACATGATATTAATAACGGAGTGACTATGTTACaa                                                   >  1:414592/1‑149 (MQ=255)
                                                                                                     cacTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGa                                                  <  2:43819/150‑1 (MQ=255)
                                                                                                          attgttACCTTCTGACAGAACAATCGCCTCTTTGTGGTCCCGGTAATCATGACCGGTACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTc                                             <  1:1759190/148‑1 (MQ=255)
                                                                                                            tgtgACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCgg                                           <  2:1016685/150‑1 (MQ=255)
                                                                                                              ttaCCTTCTCACAGAACCATCCGGTCTCTGTGTCCGCGGTCTTCATGCCCGGGACCCGGACGGGCGCAACGGATCTTCAACGTCACTTTCGCTTGCATTAACAATAACATGATATTCATCACGGAGTGACTATTTTACAGATAGTCGgcg                                         <  2:148377/148‑1 (MQ=255)
                                                                                                              tgACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGCCCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATACCATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGgcg                                         >  1:28781/1‑150 (MQ=255)
                                                                                                               gACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGCCCGGCGCAACGGATCTTCAACGCCACAATCGCTGGCATTAACAATTACATGATATTCATGACGGTGTGACGATGTTACAGATAGTCGgcgc                                        >  2:678913/1‑150 (MQ=255)
                                                                                                               cactttCTGACAGAACCAGCGGCTCTCTGTGGGCCCTGCCTTCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTTACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGgcgc                                        <  2:743541/146‑1 (MQ=255)
                                                                                                                aCCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGgcgcg                                       <  2:853089/150‑1 (MQ=255)
                                                                                                                  cTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCt                                     <  2:1026442/150‑1 (MQ=255)
                                                                                                                     gggaaacaaCCATCGGCTATCTGTGGTCCCGGACATCATGTCCGGGACCCGGTCCGGCGCAACGGATCTTCAACGCCACATTCGCTGCCATTCAGAATTTCATTATATTGATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGAt                                  <  1:139472/143‑1 (MQ=255)
                                                                                                                     cTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGAt                                  <  2:1765268/150‑1 (MQ=255)
                                                                                                                      tGACAGAACCATCGCCTCTCTGTGGACCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATc                                 <  1:370083/150‑1 (MQ=255)
                                                                                                                       gACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATcc                                <  1:1186338/150‑1 (MQ=255)
                                                                                                                       gACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATcc                                >  2:1074748/1‑150 (MQ=255)
                                                                                                                        aCAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCct                               <  1:443001/150‑1 (MQ=255)
                                                                                                                         cAGAACCATCGCCTCTCTGTGGTCCCCGGCATCATGACCGGGCCCCCGACCCGCGCAACGGCACGTCAACGCCCCAATAGCAGGCAATAAAAATAACAAGATATTAAAAACGGAGTGACAATGTTAAAGAAAGTAGGCGGGCTTATActg                              >  2:1727296/1‑150 (MQ=255)
                                                                                                                           gAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCctgct                            >  1:1061480/1‑150 (MQ=255)
                                                                                                                           gAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCctgct                            <  2:1371269/150‑1 (MQ=255)
                                                                                                                           caaCCTTCGCCGGTCTGGGTCCCCGGTAATCTTGCCCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCctgct                            <  2:1724155/149‑1 (MQ=255)
                                                                                                                             cccATCGCCTCTCTTTGGTCCCGGTAATCATGACGGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGa                          <  1:681855/149‑1 (MQ=255)
                                                                                                                             aCCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGa                          >  1:1304617/1‑150 (MQ=255)
                                                                                                                             aCCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGa                          >  1:1163823/1‑150 (MQ=255)
                                                                                                                             aCCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGa                          >  1:1311828/1‑150 (MQ=255)
                                                                                                                              ccATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGGCCCGGGCCGGGGCAACGGATCATCAACGCCACATTCGCTGGCATTAGACATAACATGATATTAATACCGGTGTGACTATGTTGCATATAGTCGGCGCGCTGATCCGGCTGAt                         >  2:360857/1‑150 (MQ=255)
                                                                                                                              ccATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGAt                         >  1:23673/1‑150 (MQ=255)
                                                                                                                              ccATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCGTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGTGTGACTATGTTACAGATAGTCGGAGCGCTGATCctgcggct                         >  2:286849/1‑146 (MQ=255)
                                                                                                                              ccATCGCCTCTCTGGGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGAt                         <  1:1696664/150‑1 (MQ=255)
                                                                                                                              ccAGCGCCTCTCTGTGGTCCCGGTCATCAGCCCCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGAt                         <  1:1689076/150‑1 (MQ=255)
                                                                                                                                aTCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATCg                       <  2:1103426/150‑1 (MQ=255)
                                                                                                                                aTCGCCTCTCTGTGGTCCCGGGCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGTCGCGCTGATCCTGCTGATCg                       <  2:946187/150‑1 (MQ=255)
                                                                                                                                   gCCTCTCTGTGGTCCCCGTCATAAATACCCGGGCCCCGACCGGCGCAACGGGTCTTGAACCCCCCATTGGCGGGCATTAACAATAACAATATATTAATCACGGAGTGACTATGTTTCAGAAAGTAGGCGCGCTGATCCATCAGATGGCAg                    >  2:391883/1‑150 (MQ=255)
                                                                                                                                    ccTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATCGCAgg                   >  1:1123737/1‑150 (MQ=255)
                                                                                                                                      tctctGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATCGCAGGAt                 >  2:1371671/1‑150 (MQ=255)
                                                                                                                                         ctGTGGTCCCGGTCATCATGACCGGGCCCCGGCCCGGCGCAACGGATCTTAAACGCCACATTCGCGGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTAAAGATAGTCGGCGCGCTGATCCTGCTGATCGCAGGATTTg              >  2:1474268/1‑150 (MQ=255)
                                                                                                                                            tgGTCCGGGTATTCTTGACGGGTACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATCGCAGGATTTGcct           <  2:1605179/150‑2 (MQ=255)
                                                                                                                                            tgGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATCGCAGGATTTGCCa           >  1:521661/1‑150 (MQ=255)
                                                                                                                                             ggTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACTATAACATGATATTCATCACGGAGTGCCTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATCGCAGGATTTGCCAt          >  1:769540/1‑150 (MQ=255)
                                                                                                                                              gTCCCGGTCATGATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATTGTCGGCGCGCTGATCCTGCTGATCGCAGGATTTGCCAtt         >  2:196397/1‑150 (MQ=255)
                                                                                                                                                cccGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATACTGCTGATCGCAGGATTTGCCTttct       >  1:372085/1‑150 (MQ=255)
                                                                                                                                                  cGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATCGCAGGATTTGCCAttcttc     <  2:414807/150‑1 (MQ=255)
                                                                                                                                                     tcatcaTGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATCGCAGGATTTGCCATTCTTCGcc  <  1:1367292/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ACTGGAAGCCCGTGTCCGGGAAAATATCACCCTGGGCGTTCAGGCCGGTTATGCCCACAGCGTCAGCGGCAGCAGCGCTGAAGGGTATAACGGTCAGGCCACACTGAATGTGACCTTCTGACAGAACCATCGCCTCTCTGTGGTCCCGGTCATCATGACCGGGACCCGGACCGGCGCAACGGATCTTCAACGCCACATTCGCTGGCATTAACAATAACATGATATTCATCACGGAGTGACTATGTTACAGATAGTCGGCGCGCTGATCCTGCTGATCGCAGGATTTGCCATTCTTCGCC  >  NC_000913/2074538‑2074836

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: