Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,115,847 G→T 5.3% A17E (GCG→GAG)  wcaM ← colanic acid biosynthesis protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,115,8470GT 5.3% 347.5 / 8.3 122A17E (GCG→GAG) wcaMcolanic acid biosynthesis protein
Reads supporting (aligned to +/- strand):  ref base G (52/62);  new base T (4/3);  total (57/65)
Fisher's exact test for biased strand distribution p-value = 7.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.85e-01

Only 100 of 125 total aligned reads displayed.

AAACCAATCCGGCAGGCACGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTGACGA  >  NC_000913/2115698‑2115995
                                                                                                                                                     |                                                                                                                                                     
aaaCCAATCCGGCAGGCACGACTACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAgcg                                                                                                                                                       <  2:1316182/150‑1 (MQ=255)
   ccAATCCGGCAGGCACGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCg                                                                                                                                                    >  1:619624/1‑150 (MQ=255)
   ccAATCCGGCAGGCACGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCg                                                                                                                                                    <  2:1490186/150‑1 (MQ=255)
   ccAATCCGGCAGGCACGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGGGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGGGGGCGAAAGGGGTATGGAGGAAGGCAAGCGccc                                                                                                                                                    >  1:673469/1‑149 (MQ=255)
      aTCCGGCAGGCACGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGCGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGc                                                                                                                                                 <  1:1871553/150‑1 (MQ=255)
        ccGGCAGGCACGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTAGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCtt                                                                                                                                               >  2:1795061/1‑150 (MQ=255)
             aggcaCGCCGACTGCTTGCCCTTCGCTGCAGGCTGGTTAAAATGAGGCGATCCAGTCGTGGGGGTTGTAGTCGTTAATGTTTACTCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGACGGCAAGCTCGGCGCTTGcctt                                                                                                                                          <  1:1327660/150‑3 (MQ=255)
             aggcaCGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCTCGGCGCTTGcctt                                                                                                                                          <  2:1324638/150‑3 (MQ=255)
              ggcaCGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGCGGCGATCCAGTCGTGTGGGTGGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTc                                                                                                                                         <  1:1180433/150‑1 (MQ=255)
              ggcaCGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTc                                                                                                                                         >  2:1241469/1‑150 (MQ=255)
               gcaCGACGACTGTTTGCCCGGCGCTGAAGGCTTGTTTAACGGAGGCGATCCAGGCGTGGGGGTTGGAGCCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTAGGGAGGAAGGCAAGCGCCGAGCTTGCCGTCa                                                                                                                                        <  1:1845236/150‑1 (MQ=255)
                caCGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAg                                                                                                                                       <  2:1493622/150‑1 (MQ=255)
                  cgacgaCTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGGCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCTCGGCGCTTGCCTTcctcc                                                                                                                                     <  1:742416/150‑5 (MQ=255)
                          gTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCCTTTATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGCAGGCAAGCGCCGACCATTCCGGCAGGAACGTTCgg                                                                                                                             >  2:184553/1‑149 (MQ=255)
                            ttGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTTGTGGTTAATGTTTACCGTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGGATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCg                                                                                                                           >  1:875326/1‑150 (MQ=255)
                                     gCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTTATGTTAACGCTTTGGCGGGCGGGAAGCGCGCGGGCGAAAGGGGTTTGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGttt                                                                                                                  >  1:1680480/1‑150 (MQ=255)
                                     gCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTTACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTGGGGAGAGtgt                                                                                                                  >  2:1837224/1‑148 (MQ=255)
                                     gCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAAAGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGCGCGTGCCGTCAGGAACGTGCGTCGGGAGAGttt                                                                                                                  >  1:1782986/1‑150 (MQ=255)
                                     gCTGAAGGCTTGTTTAAATGAGGAGATCCAGTCGTGTGGGATGTAGTCGTTAATGTTAAAGCGTTGTCGGGCGGGATGCCCGCGCGCCAAACGGGTATCGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGttt                                                                                                                  >  2:368335/1‑150 (MQ=255)
                                       tGAAGGCTTGTTTAAATGAGGCGATCCAGTCGGGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGttttt                                                                                                                <  2:1839615/150‑1 (MQ=255)
                                        gAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTAGGTAATGTTTAAGGTTTTTCGGGCGGGAAGCGGGCGGGCGAAACGGGTATTGAGGAAGGCAAGCGCCGAGCATGCCCTCAGGCACGTGCGGCGGGAGAGgttttt                                                                                                               >  1:1581710/1‑150 (MQ=255)
                                         aaGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTa                                                                                                              <  2:509961/150‑1 (MQ=255)
                                          aGGGTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTTATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTaa                                                                                                             <  1:1221080/150‑1 (MQ=255)
                                           ggCTTGTTTAACTGACGCGATCCAGTCGTGTGGGGTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTGTTTTaaa                                                                                                            <  1:1392588/150‑1 (MQ=255)
                                             cTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATg                                                                                                          <  1:1442731/150‑1 (MQ=255)
                                             cTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAAAGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCCCCGAGCATGCCGTCAGGTACGTGCGTCGGGAGAGTTTTTTAAATg                                                                                                          >  2:163085/1‑150 (MQ=255)
                                              ttGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATgg                                                                                                         <  1:1694227/150‑1 (MQ=255)
                                              ttGGTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGTAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAAttg                                                                                                         >  1:1061070/1‑148 (MQ=255)
                                                gTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTTATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCa                                                                                                       >  2:1285696/1‑150 (MQ=255)
                                                gTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCa                                                                                                       >  1:1018014/1‑150 (MQ=255)
                                                gTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCa                                                                                                       <  1:432278/150‑1 (MQ=255)
                                                 tttAAATGAGGCGATCCAGTCGTGTGGGGTGTAGTCGGTAATGTTTACGCTTTGTAGGGCGGGAAGCGCGCGGGGGAAAGGGGGATGGAGGAAGGCAAGCGCCGAGCTTGGCGGCAGGCACCGGCGTGGGGAGTGGTTTTTTAATGGCAt                                                                                                      >  2:1468892/1‑150 (MQ=255)
                                                   tAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTTATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACCTGCGTCGGGAGAGTTTTTTTAATGGCATGc                                                                                                    >  2:1818111/1‑150 (MQ=255)
                                                    aaaTGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCg                                                                                                   >  1:1081592/1‑150 (MQ=255)
                                                    aaaTGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCg                                                                                                   >  2:1175068/1‑150 (MQ=255)
                                                     aaTGAGGCGATCCAGTCGTGTGGGTTGTAGGCGTTAATGTTAACGCTTTGGCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGt                                                                                                  <  1:831280/150‑1 (MQ=255)
                                                      aTGAGGCGATCCAGTCGTGTGGGTTGTTGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGtt                                                                                                 <  1:1236369/150‑1 (MQ=255)
                                                       tGAGGCGATCCAGTCGTGTGGGTTGTAGGCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAATGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTtc                                                                                                <  1:231239/150‑1 (MQ=255)
                                                            cGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTctcctc                                                                                           <  1:764375/150‑1 (MQ=255)
                                                              acccAGTCCTGGGGGCTGCAGTCGTTAAAGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCta                                                                                         <  2:138530/148‑1 (MQ=255)
                                                               tCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGGTCTCCTCtat                                                                                        >  1:1141787/1‑150 (MQ=255)
                                                                     cGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGcc                                                                                  <  2:1895243/150‑1 (MQ=255)
                                                                      gtgtGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCt                                                                                 >  1:685044/1‑150 (MQ=255)
                                                                      gtgtGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCt                                                                                 <  1:946224/150‑1 (MQ=255)
                                                                        gtgGGTTGTAGTCGTTACTGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTgc                                                                               <  2:1758175/150‑1 (MQ=255)
                                                                        gtgGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTgc                                                                               >  2:95634/1‑150 (MQ=255)
                                                                        gtgGGCTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTgc                                                                               <  2:106699/150‑1 (MQ=255)
                                                                        gggggTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGTGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTgc                                                                               <  1:894049/148‑1 (MQ=255)
                                                                         ggggTTGTAGTAGTTAATGTTAACGATTTGGCGGGCTGGAAGCGCGCGGGCGAAAGGTGTATGGAGGAAGGCAATCGCCGAGCTTTCAGTCAGGAACGTGCGTGGTGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTgca                                                                              <  1:267524/149‑1 (MQ=255)
                                                                          gggTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGCAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTgcag                                                                             >  2:764934/1‑150 (MQ=255)
                                                                           ggTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTgcagc                                                                            >  2:1609103/1‑150 (MQ=255)
                                                                             ttGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCaa                                                                          >  1:839775/1‑150 (MQ=255)
                                                                                tAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGCGCTTGCCGTCAGGAACGTGCGTCCGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAACGCCTGCAGCAAGCt                                                                       >  1:298371/1‑150 (MQ=255)
                                                                                  gTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCTTCTGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTgg                                                                     >  2:231561/1‑150 (MQ=255)
                                                                                   tCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGc                                                                    <  2:416554/150‑1 (MQ=255)
                                                                                    ggTTAATGTTACCGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCg                                                                   <  1:467174/149‑1 (MQ=255)
                                                                                       tAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGt                                                                <  2:1058704/150‑1 (MQ=255)
                                                                                          tGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTATTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTtct                                                             >  1:482661/1‑150 (MQ=255)
                                                                                           gTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTtctc                                                            <  1:511274/150‑1 (MQ=255)
                                                                                               aCGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGAtt                                                        >  1:1734297/1‑150 (MQ=255)
                                                                                                cGCTTTGTCGGTCGGGAAGCGCGCGGGCGAAAGGGGTCTGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCTTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTg                                                       <  1:1424413/150‑1 (MQ=255)
                                                                                                   tttGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATc                                                    <  2:912336/150‑1 (MQ=255)
                                                                                                   tttGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATc                                                    <  2:1498509/150‑1 (MQ=255)
                                                                                                      gTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCAcc                                                 <  2:619624/150‑1 (MQ=255)
                                                                                                        cgggcgggAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACctg                                               >  1:814991/1‑150 (MQ=255)
                                                                                                         gggcgggAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACctgc                                              <  1:1260013/150‑1 (MQ=255)
                                                                                                              ggAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTCTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGtt                                         <  2:482661/150‑1 (MQ=255)
                                                                                                                acgcgcgcGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTaa                                      <  2:1459254/148‑1 (MQ=255)
                                                                                                                     cgcgGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCg                                 <  1:1795061/150‑1 (MQ=255)
                                                                                                                     cgcgGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCg                                 >  1:1368595/1‑150 (MQ=255)
                                                                                                                       cgGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCgtg                               <  2:383582/150‑1 (MQ=255)
                                                                                                                       cgGGCGAAAGGGGTATGGAGGAAGGAAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCgtg                               <  2:1416292/150‑1 (MQ=255)
                                                                                                                          gCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTc                            <  2:1017912/150‑1 (MQ=255)
                                                                                                                          gCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGAGTTc                            >  1:1488498/1‑150 (MQ=255)
                                                                                                                             aaaGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAAc                         <  1:1180983/150‑1 (MQ=255)
                                                                                                                              aaGGGGTCTGGAGTAAGGCAAGCGCCGAGCTTGCCGTCAGGATCTTGCGGCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTTCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACt                        <  1:1791373/150‑1 (MQ=255)
                                                                                                                                 gggTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACtttt                     >  2:1841449/1‑150 (MQ=255)
                                                                                                                                  ggTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATTCGTTCTCCTCTATAAAGCCTTCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACttttt                    >  2:159084/1‑150 (MQ=255)
                                                                                                                                  ggTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACttttt                    >  1:1338208/1‑150 (MQ=255)
                                                                                                                                    tATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTcg                  >  1:1668006/1‑150 (MQ=255)
                                                                                                                                    tATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTGCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTcg                  <  1:1301692/150‑1 (MQ=255)
                                                                                                                                       ggaggaAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTcgcgc               >  2:490390/1‑150 (MQ=255)
                                                                                                                                       ggaggaAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTAAAAATCGTGTTCAACTTTTTcgcgc               <  2:956381/150‑1 (MQ=255)
                                                                                                                                        cctgacggCAAGCTCGGCGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTcgcgcg              >  1:1509068/7‑150 (MQ=255)
                                                                                                                                          ggaAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGTCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTcgcgcgcg            >  2:1325505/1‑150 (MQ=255)
                                                                                                                                          ggaAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTcgcgcgcg            <  1:1285696/150‑1 (MQ=255)
                                                                                                                                           gacggCAAGCTCGGCGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTcgcgcgcgc           >  1:1648303/4‑150 (MQ=255)
                                                                                                                                           gacggCAAGCTCGGCGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTcgcgcgcgc           >  2:1515008/4‑150 (MQ=255)
                                                                                                                                           gaAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTcgcgcgcgc           >  1:1885115/1‑150 (MQ=255)
                                                                                                                                            acggCAAGCTCGGCGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCGGCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTcgcgcgcgcg          >  1:35556/3‑150 (MQ=255)
                                                                                                                                            aaGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTcgcgcgcgcg          >  2:904570/1‑150 (MQ=255)
                                                                                                                                               gCAAGCGCCGAGCTTGCCGGCAGGAACGGGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGttt       <  2:1710542/150‑1 (MQ=255)
                                                                                                                                                cAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTATAATCGGGTTGAACTTTTTCTCGCGGGCGTTTg      >  2:1551715/1‑150 (MQ=255)
                                                                                                                                                cAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTg      >  1:1574798/1‑150 (MQ=255)
                                                                                                                                                cAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTg      <  2:433754/150‑1 (MQ=255)
                                                                                                                                                cAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTg      >  1:1254444/1‑150 (MQ=255)
                                                                                                                                                  aGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTga     <  2:1018014/150‑1 (MQ=255)
                                                                                                                                                  aGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTgac    >  1:1533183/1‑150 (MQ=255)
                                                                                                                                                   gcgcCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGAGTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTgacg   >  1:1002619/1‑150 (MQ=255)
                                                                                                                                                    cgcCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTgacgc  >  2:813038/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AAACCAATCCGGCAGGCACGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTGACGA  >  NC_000913/2115698‑2115995

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: