Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,115,849 C→G 5.7% S16S (TCG→TCC) ‡ wcaM ← colanic acid biosynthesis protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,115,8490CG 5.7% 386.7 / 12.8 123S16S (TCG→TCC) ‡wcaMcolanic acid biosynthesis protein
Reads supporting (aligned to +/- strand):  ref base C (54/62);  new base G (4/3);  total (58/65)
Fisher's exact test for biased strand distribution p-value = 7.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.30e-01

Only 100 of 127 total aligned reads displayed.

CCAATCCGGCAGGCACGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTGACGACCG  >  NC_000913/2115701‑2115998
                                                                                                                                                    |                                                                                                                                                     
ccAATCCGGCAGGCACGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCg                                                                                                                                                      >  1:619624/1‑150 (MQ=255)
ccAATCCGGCAGGCACGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCg                                                                                                                                                      <  2:1490186/150‑1 (MQ=255)
ccAATCCGGCAGGCACGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGGGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGGGGGCGAAAGGGGTATGGAGGAAGGCAAGCGccc                                                                                                                                                      >  1:673469/1‑149 (MQ=255)
   aTCCGGCAGGCACGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGCGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGc                                                                                                                                                   <  1:1871553/150‑1 (MQ=255)
    tCCGGCAGGCACGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCt                                                                                                                                                  <  1:1385814/150‑1 (MQ=255)
          aggcaCGCCGACTGCTTGCCCTTCGCTGCAGGCTGGTTAAAATGAGGCGATCCAGTCGTGGGGGTTGTAGTCGTTAATGTTTACTCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGACGGCAAGCTCGGCGCTTGcctt                                                                                                                                            <  1:1327660/150‑3 (MQ=255)
          aggcaCGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCTCGGCGCTTGcctt                                                                                                                                            <  2:1324638/150‑3 (MQ=255)
          aggcaCGACGACTGTTTGCCCTGCGCTGAAGGCGTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGt                                                                                                                                            <  2:506275/150‑1 (MQ=255)
           ggcaCGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGCGGCGATCCAGTCGTGTGGGTGGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTc                                                                                                                                           <  1:1180433/150‑1 (MQ=255)
             caCGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAg                                                                                                                                         <  2:1493622/150‑1 (MQ=255)
               cgacgaCTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGGCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCTCGGCGCTTGCCTTcctcc                                                                                                                                       <  1:742416/150‑5 (MQ=255)
                  cgacTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACg                                                                                                                                    <  2:1471882/150‑1 (MQ=255)
                       gTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCCTTTATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGCAGGCAAGCGCCGACCATTCCGGCAGGAACGTTCgg                                                                                                                               >  2:184553/1‑149 (MQ=255)
                                  gCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTTATGTTAACGCTTTGGCGGGCGGGAAGCGCGCGGGCGAAAGGGGTTTGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGttt                                                                                                                    >  1:1680480/1‑150 (MQ=255)
                                  gCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTTACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTGGGGAGAGtgt                                                                                                                    >  2:1837224/1‑148 (MQ=255)
                                  gCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGGGGGGTGTTGTCGTTAATGTTAACGCGTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGGATGGAGGAAGGCCAGCGCCGCGCGTGCCGGCAGGGCCGGGCGTGGGGGGAGttt                                                                                                                    >  2:1836224/1‑150 (MQ=255)
                                    tGAAGGCTTGTTTAAATGAGGCGATCCAGTCGGGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGttttt                                                                                                                  <  2:1839615/150‑1 (MQ=255)
                                      aaGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTa                                                                                                                <  2:509961/150‑1 (MQ=255)
                                       aGGGTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTTATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTaa                                                                                                               <  1:1221080/150‑1 (MQ=255)
                                        ggCTTGTTTAACTGACGCGATCCAGTCGTGTGGGGTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTGTTTTaaa                                                                                                              <  1:1392588/150‑1 (MQ=255)
                                          cTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATg                                                                                                            <  1:1442731/150‑1 (MQ=255)
                                           ttGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATgg                                                                                                           <  1:1694227/150‑1 (MQ=255)
                                           ttGGTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGTAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAAttg                                                                                                           >  1:1061070/1‑148 (MQ=255)
                                             gTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTTATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCa                                                                                                         >  2:1285696/1‑150 (MQ=255)
                                             gTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCa                                                                                                         <  1:432278/150‑1 (MQ=255)
                                             gTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCa                                                                                                         >  1:1018014/1‑150 (MQ=255)
                                                tAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTTATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACCTGCGTCGGGAGAGTTTTTTTAATGGCATGc                                                                                                      >  2:1818111/1‑150 (MQ=255)
                                                 aaaTGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCg                                                                                                     >  2:1175068/1‑150 (MQ=255)
                                                 aaaTGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCg                                                                                                     >  1:1081592/1‑150 (MQ=255)
                                                  aaTGAGGCGATCCCGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGGCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGTAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGt                                                                                                    <  1:1499810/150‑1 (MQ=255)
                                                  aaTGAGGCGATCCAGTCGTGTGGGTTGTAGGCGTTAATGTTAACGCTTTGGCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGt                                                                                                    <  1:831280/150‑1 (MQ=255)
                                                   aTGAGGCGATCCAGTCGTGTGGGTTGTTGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGtt                                                                                                   <  1:1236369/150‑1 (MQ=255)
                                                    tGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTtc                                                                                                  <  2:1524353/150‑1 (MQ=255)
                                                    tGAGGCGATCCAGTCGTGTGGGTTGTAGGCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAATGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTtc                                                                                                  <  1:231239/150‑1 (MQ=255)
                                                       ggCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTctcc                                                                                               <  2:411247/150‑1 (MQ=255)
                                                       ggCGATCCAGTCGTGTGGGTTGTAGGCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTctcc                                                                                               <  1:875881/150‑1 (MQ=255)
                                                         cGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTctcctc                                                                                             <  1:764375/150‑1 (MQ=255)
                                                           acccAGTCCTGGGGGCTGCAGTCGTTAAAGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCta                                                                                           <  2:138530/148‑1 (MQ=255)
                                                            tCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGGTCTCCTCtat                                                                                          >  1:1141787/1‑150 (MQ=255)
                                                             ccAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCtata                                                                                         <  2:1370931/150‑1 (MQ=255)
                                                                  cGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGcc                                                                                    <  2:1895243/150‑1 (MQ=255)
                                                                   gtgtGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCt                                                                                   >  1:685044/1‑150 (MQ=255)
                                                                   gtgtGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCt                                                                                   <  1:946224/150‑1 (MQ=255)
                                                                     gtgGGTTGTAGTCGTTACTGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTgc                                                                                 <  2:1758175/150‑1 (MQ=255)
                                                                     gtgGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTgc                                                                                 >  2:95634/1‑150 (MQ=255)
                                                                     gtgGGCTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTgc                                                                                 <  2:106699/150‑1 (MQ=255)
                                                                     gggggTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGTGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTgc                                                                                 <  1:894049/148‑1 (MQ=255)
                                                                       gggTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGCAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTgcag                                                                               >  2:764934/1‑150 (MQ=255)
                                                                        ggTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTgcagc                                                                              >  2:1609103/1‑150 (MQ=255)
                                                                          ttGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCaa                                                                            >  1:839775/1‑150 (MQ=255)
                                                                             tAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGCGCTTGCCGTCAGGAACGTGCGTCCGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAACGCCTGCAGCAAGCt                                                                         >  1:298371/1‑150 (MQ=255)
                                                                               gTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCTTCTGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTgg                                                                       >  2:231561/1‑150 (MQ=255)
                                                                                tCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGc                                                                      <  2:416554/150‑1 (MQ=255)
                                                                                 ggTTAATGTTACCGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCg                                                                     <  1:467174/149‑1 (MQ=255)
                                                                                    tAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGt                                                                  <  2:1058704/150‑1 (MQ=255)
                                                                                       tGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTATTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTtct                                                               >  1:482661/1‑150 (MQ=255)
                                                                                        gTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTtctc                                                              >  2:1031296/1‑150 (MQ=255)
                                                                                        gTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTtctc                                                              <  1:511274/150‑1 (MQ=255)
                                                                                          tAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGa                                                            >  1:1584108/1‑150 (MQ=255)
                                                                                            aCGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGAtt                                                          >  1:1734297/1‑150 (MQ=255)
                                                                                             cGCTTTGTCGGTCGGGAAGCGCGCGGGCGAAAGGGGTCTGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCTTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTg                                                         <  1:1424413/150‑1 (MQ=255)
                                                                                               cTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGAt                                                       >  2:1244920/1‑150 (MQ=255)
                                                                                                tttGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATc                                                      <  2:912336/150‑1 (MQ=255)
                                                                                                tttGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATc                                                      <  2:1498509/150‑1 (MQ=255)
                                                                                                     cgggcgggAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACctg                                                 >  1:814991/1‑150 (MQ=255)
                                                                                                      gggcgggAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACctgc                                                <  1:1260013/150‑1 (MQ=255)
                                                                                                           ggAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTCTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGtt                                           <  2:482661/150‑1 (MQ=255)
                                                                                                            gAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTa                                          >  2:1691122/1‑150 (MQ=255)
                                                                                                             acgcgcgcGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTaa                                         <  2:1459254/148‑1 (MQ=255)
                                                                                                              aGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTaaa                                        <  1:245776/150‑1 (MQ=255)
                                                                                                                  cgcgGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCg                                    <  1:1795061/150‑1 (MQ=255)
                                                                                                                    cgGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCgtg                                  <  2:383582/150‑1 (MQ=255)
                                                                                                                    cgGGCGAAAGGGGTATGGAGGAAGGAAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCgtg                                  <  2:1416292/150‑1 (MQ=255)
                                                                                                                      ggCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGtt                                >  2:844539/1‑150 (MQ=255)
                                                                                                                       gCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTc                               <  2:1017912/150‑1 (MQ=255)
                                                                                                                       gCGAAAGGGGTAGGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTc                               <  2:1862448/150‑1 (MQ=255)
                                                                                                                          aaaGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAAc                            <  1:1180983/150‑1 (MQ=255)
                                                                                                                           aaGGGGTCTGGAGTAAGGCAAGCGCCGAGCTTGCCGTCAGGATCTTGCGGCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTTCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACt                           <  1:1791373/150‑1 (MQ=255)
                                                                                                                               ggTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATTCGTTCTCCTCTATAAAGCCTTCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACttttt                       >  2:159084/1‑150 (MQ=255)
                                                                                                                               ggTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACttttt                       >  1:1338208/1‑150 (MQ=255)
                                                                                                                                 tATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTGCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTcg                     <  1:1301692/150‑1 (MQ=255)
                                                                                                                                    ggaggaAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTcgcgc                  >  2:490390/1‑150 (MQ=255)
                                                                                                                                    ggaggaAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTAAAAATCGTGTTCAACTTTTTcgcgc                  <  2:956381/150‑1 (MQ=255)
                                                                                                                                     cctgacggCAAGCTCGGCGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTcgcgcg                 >  1:1509068/7‑150 (MQ=255)
                                                                                                                                       ggaAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTcgcgcgcg               <  1:1285696/150‑1 (MQ=255)
                                                                                                                                        gacggCAAGCTCGGCGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTcgcgcgcgc              >  1:1648303/4‑150 (MQ=255)
                                                                                                                                        gaAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTcgcgcgcgc              >  1:1885115/1‑150 (MQ=255)
                                                                                                                                         acggCAAGCTCGGCGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCGGCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTcgcgcgcgcg             >  1:35556/3‑150 (MQ=255)
                                                                                                                                            gCAAGCGCCGAGCTTGCCGGCAGGAACGGGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGttt          <  2:1710542/150‑1 (MQ=255)
                                                                                                                                             cAAGCGCCGAGCTTGCCGTCATGAACGTGCTTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGCTCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTg         <  1:170668/150‑1 (MQ=255)
                                                                                                                                             cAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTATAATCGGGTTGAACTTTTTCTCGCGGGCGTTTg         >  2:1551715/1‑150 (MQ=255)
                                                                                                                                             cAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTg         >  1:1254444/1‑150 (MQ=255)
                                                                                                                                             cAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTg         >  1:1574798/1‑150 (MQ=255)
                                                                                                                                              aaGCGCCGAGCTTGCCGTCAGGAACGTGCGGCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTga        <  1:1836224/150‑1 (MQ=255)
                                                                                                                                               aGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTgac       >  1:1533183/1‑150 (MQ=255)
                                                                                                                                                gcgcCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGAGTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTgacg      >  1:1002619/1‑150 (MQ=255)
                                                                                                                                                 cgcCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTgacgc     >  2:813038/1‑149 (MQ=255)
                                                                                                                                                   ccGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTGACGAcc   >  1:1635018/1‑150 (MQ=255)
                                                                                                                                                    cGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTGACGCCCg  >  1:1837087/1‑150 (MQ=255)
                                                                                                                                                    cGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTGACGACCg  <  2:673469/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CCAATCCGGCAGGCACGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTGACGACCG  >  NC_000913/2115701‑2115998

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: