Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,115,851 A→C 7.5% S16A (TCG→GCG) ‡ wcaM ← colanic acid biosynthesis protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,115,8510AC 7.5% 346.4 / 12.5 124S16A (TCG→GCG) ‡wcaMcolanic acid biosynthesis protein
Reads supporting (aligned to +/- strand):  ref base A (52/62);  new base C (7/3);  total (59/65)
Fisher's exact test for biased strand distribution p-value = 1.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.02e-01

Only 100 of 124 total aligned reads displayed.

ATCCGGCAGGCACGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTGACGACCG  >  NC_000913/2115704‑2115998
                                                                                                                                                   |                                                                                                                                                    
aTCCGGCAGGCACGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGCGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGc                                                                                                                                                    <  1:1871553/150‑1 (MQ=255)
 tCCGGCAGGCACGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCt                                                                                                                                                   <  1:1385814/150‑1 (MQ=255)
  ccGGCAGGCACGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTAGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCtt                                                                                                                                                  >  2:1795061/1‑150 (MQ=255)
   cGGCAGGCACGACGCCTGCTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGGCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTg                                                                                                                                                 <  1:1294482/150‑1 (MQ=255)
       aggcaCGCCGACTGCTTGCCCTTCGCTGCAGGCTGGTTAAAATGAGGCGATCCAGTCGTGGGGGTTGTAGTCGTTAATGTTTACTCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGACGGCAAGCTCGGCGCTTGcctt                                                                                                                                             <  1:1327660/150‑3 (MQ=255)
       aggcaCGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCTCGGCGCTTGcctt                                                                                                                                             <  2:1324638/150‑3 (MQ=255)
       aggcaCGACGACTGTTTGCCCTGCGCTGAAGGCGTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGt                                                                                                                                             <  2:506275/150‑1 (MQ=255)
        ggcaCGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGCGGCGATCCAGTCGTGTGGGTGGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTc                                                                                                                                            <  1:1180433/150‑1 (MQ=255)
        ggcaCGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTc                                                                                                                                            >  2:1241469/1‑150 (MQ=255)
         gcaCGACGACTGTTTGCCCGGCGCTGAAGGCTTGTTTAACGGAGGCGATCCAGGCGTGGGGGTTGGAGCCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTAGGGAGGAAGGCAAGCGCCGAGCTTGCCGTCa                                                                                                                                           <  1:1845236/150‑1 (MQ=255)
          caCGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAg                                                                                                                                          <  2:1493622/150‑1 (MQ=255)
            cgacgaCTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGGCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCTCGGCGCTTGCCTTcctcc                                                                                                                                        <  1:742416/150‑5 (MQ=255)
               cgacTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACg                                                                                                                                     <  2:1471882/150‑1 (MQ=255)
                    gTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCCTTTATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGCAGGCAAGCGCCGACCATTCCGGCAGGAACGTTCgg                                                                                                                                >  2:184553/1‑149 (MQ=255)
                      ttGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTTGTGGTTAATGTTTACCGTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGGATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCg                                                                                                                              >  1:875326/1‑150 (MQ=255)
                               gCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTTATGTTAACGCTTTGGCGGGCGGGAAGCGCGCGGGCGAAAGGGGTTTGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGttt                                                                                                                     >  1:1680480/1‑150 (MQ=255)
                               gCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAAAGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGCGCGTGCCGTCAGGAACGTGCGTCGGGAGAGttt                                                                                                                     >  1:1782986/1‑150 (MQ=255)
                               gCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGGGGGGTGTTGTCGTTAATGTTAACGCGTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGGATGGAGGAAGGCCAGCGCCGCGCGTGCCGGCAGGGCCGGGCGTGGGGGGAGttt                                                                                                                     >  2:1836224/1‑150 (MQ=255)
                               gCTGAAGGCTTGTTTAAATGAGGAGATCCAGTCGTGTGGGATGTAGTCGTTAATGTTAAAGCGTTGTCGGGCGGGATGCCCGCGCGCCAAACGGGTATCGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGttt                                                                                                                     >  2:368335/1‑150 (MQ=255)
                                 tGAAGGCTTGTTTAAATGAGGCGATCCAGTCGGGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGttttt                                                                                                                   <  2:1839615/150‑1 (MQ=255)
                                  gAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTAGGTAATGTTTAAGGTTTTTCGGGCGGGAAGCGGGCGGGCGAAACGGGTATTGAGGAAGGCAAGCGCCGAGCATGCCCTCAGGCACGTGCGGCGGGAGAGgttttt                                                                                                                  >  1:1581710/1‑150 (MQ=255)
                                    aGGGTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTTATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTaa                                                                                                                <  1:1221080/150‑1 (MQ=255)
                                       cTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATg                                                                                                             <  1:1442731/150‑1 (MQ=255)
                                       cTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAAAGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCCCCGAGCATGCCGTCAGGTACGTGCGTCGGGAGAGTTTTTTAAATg                                                                                                             >  2:163085/1‑150 (MQ=255)
                                        ttGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATgg                                                                                                            <  1:1694227/150‑1 (MQ=255)
                                        ttGGTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGTAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAAttg                                                                                                            >  1:1061070/1‑148 (MQ=255)
                                          gTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTTATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCa                                                                                                          >  2:1285696/1‑150 (MQ=255)
                                          gTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCa                                                                                                          <  1:432278/150‑1 (MQ=255)
                                          gTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCa                                                                                                          >  1:1018014/1‑150 (MQ=255)
                                             tAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTTATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACCTGCGTCGGGAGAGTTTTTTTAATGGCATGc                                                                                                       >  2:1818111/1‑150 (MQ=255)
                                              aaaTGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCg                                                                                                      >  1:1081592/1‑150 (MQ=255)
                                               aaTGAGGCGATCCCGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGGCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGTAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGt                                                                                                     <  1:1499810/150‑1 (MQ=255)
                                                 tGAGGCGATCCAGTCGTGTGGGTTGTAGGCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAATGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTtc                                                                                                   <  1:231239/150‑1 (MQ=255)
                                                    ggCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTctcc                                                                                                <  2:411247/150‑1 (MQ=255)
                                                    ggCGATCCAGTCGTGTGGGTTGTAGGCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTctcc                                                                                                <  1:875881/150‑1 (MQ=255)
                                                      cGATCCCGTCGGGTGGGTTGGAGGCGTTAATGGTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTctcctc                                                                                              <  1:785320/150‑1 (MQ=255)
                                                      cGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTctcctc                                                                                              <  1:764375/150‑1 (MQ=255)
                                                        acccAGTCCTGGGGGCTGCAGTCGTTAAAGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCta                                                                                            <  2:138530/148‑1 (MQ=255)
                                                          ccAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCtata                                                                                          <  2:1370931/150‑1 (MQ=255)
                                                                gtgtGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCt                                                                                    <  1:946224/150‑1 (MQ=255)
                                                                  gtgGGTTGTAGTCGTTACTGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTgc                                                                                  <  2:1758175/150‑1 (MQ=255)
                                                                  gtgGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTgc                                                                                  >  2:95634/1‑150 (MQ=255)
                                                                  gtgGGCTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTgc                                                                                  <  2:106699/150‑1 (MQ=255)
                                                                  gggggTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGTGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTgc                                                                                  <  1:894049/148‑1 (MQ=255)
                                                                   ggggTTGTAGTAGTTAATGTTAACGATTTGGCGGGCTGGAAGCGCGCGGGCGAAAGGTGTATGGAGGAAGGCAATCGCCGAGCTTTCAGTCAGGAACGTGCGTGGTGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTgca                                                                                 <  1:267524/149‑1 (MQ=255)
                                                                    gggTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGCAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTgcag                                                                                >  2:764934/1‑150 (MQ=255)
                                                                     ggTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTgcagc                                                                               >  2:1609103/1‑150 (MQ=255)
                                                                       ttGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCaa                                                                             >  1:839775/1‑150 (MQ=255)
                                                                          tAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGCGCTTGCCGTCAGGAACGTGCGTCCGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAACGCCTGCAGCAAGCt                                                                          >  1:298371/1‑150 (MQ=255)
                                                                            gTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCTTCTGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTgg                                                                        >  2:231561/1‑150 (MQ=255)
                                                                              ggTTAATGTTACCGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCg                                                                      <  1:467174/149‑1 (MQ=255)
                                                                                 tAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGt                                                                   <  2:1058704/150‑1 (MQ=255)
                                                                                    tGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTATTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTtct                                                                >  1:482661/1‑150 (MQ=255)
                                                                                     gTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTtctc                                                               <  1:511274/150‑1 (MQ=255)
                                                                                     gTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTtctc                                                               >  2:1031296/1‑150 (MQ=255)
                                                                                       tAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGa                                                             >  1:1584108/1‑150 (MQ=255)
                                                                                        aaCGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGAt                                                            <  1:1123157/150‑1 (MQ=255)
                                                                                          cGCTTTGTCGGTCGGGAAGCGCGCGGGCGAAAGGGGTCTGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCTTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTg                                                          <  1:1424413/150‑1 (MQ=255)
                                                                                            cTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGAt                                                        >  2:1244920/1‑150 (MQ=255)
                                                                                             tttGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATc                                                       <  2:1498509/150‑1 (MQ=255)
                                                                                             tttGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATc                                                       <  2:912336/150‑1 (MQ=255)
                                                                                                gTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCAcc                                                    <  2:619624/150‑1 (MQ=255)
                                                                                                  cgggcgggAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACctg                                                  >  1:814991/1‑150 (MQ=255)
                                                                                                   gggcgggAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACctgc                                                 <  1:1260013/150‑1 (MQ=255)
                                                                                                        ggAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTCTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGtt                                            <  2:482661/150‑1 (MQ=255)
                                                                                                          acgcgcgcGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTaa                                         <  2:1459254/148‑1 (MQ=255)
                                                                                                               cgcgGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCg                                    <  1:1795061/150‑1 (MQ=255)
                                                                                                               cgcgGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCg                                    >  1:1368595/1‑150 (MQ=255)
                                                                                                                 cgGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCgtg                                  <  2:383582/150‑1 (MQ=255)
                                                                                                                 cgGGCGAAAGGGGTATGGAGGAAGGAAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCgtg                                  <  2:1416292/150‑1 (MQ=255)
                                                                                                                   ggCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGGTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGCTTGATCACCTGCTGGTTAAAATCGTGtt                                >  1:1226295/1‑150 (MQ=255)
                                                                                                                    gCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGAGTTc                               >  1:1488498/1‑150 (MQ=255)
                                                                                                                    gCGAAAGGGGTAGGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTc                               <  2:1862448/150‑1 (MQ=255)
                                                                                                                       aaaGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAAc                            <  1:1180983/150‑1 (MQ=255)
                                                                                                                        aaGGGGTCTGGAGTAAGGCAAGCGCCGAGCTTGCCGTCAGGATCTTGCGGCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTTCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACt                           <  1:1791373/150‑1 (MQ=255)
                                                                                                                           gggTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACtttt                        >  2:1841449/1‑150 (MQ=255)
                                                                                                                            ggTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATTCGTTCTCCTCTATAAAGCCTTCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACttttt                       >  2:159084/1‑150 (MQ=255)
                                                                                                                            ggTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACttttt                       >  1:1338208/1‑150 (MQ=255)
                                                                                                                              tATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTcg                     >  1:1668006/1‑150 (MQ=255)
                                                                                                                                 ggaggaAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTcgcgc                  >  2:490390/1‑150 (MQ=255)
                                                                                                                                 ggaggaAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTAAAAATCGTGTTCAACTTTTTcgcgc                  <  2:956381/150‑1 (MQ=255)
                                                                                                                                    ggaAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTTAAATCGTGTTCAACTTTTTcgcgcgcg               >  1:672822/1‑150 (MQ=255)
                                                                                                                                    ggaAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTcgcgcgcg               <  1:1285696/150‑1 (MQ=255)
                                                                                                                                     gacggCAAGCTCGGCGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTcgcgcgcgc              >  2:1515008/4‑150 (MQ=255)
                                                                                                                                     gacggCAAGCTCGGCGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTcgcgcgcgc              >  1:1648303/4‑150 (MQ=255)
                                                                                                                                     gaAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTcgcgcgcgc              >  1:1885115/1‑150 (MQ=255)
                                                                                                                                      acggCAAGCTCGGCGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCGGCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTcgcgcgcgcg             >  1:35556/3‑150 (MQ=255)
                                                                                                                                      aaGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTcgcgcgcgcg             >  2:904570/1‑150 (MQ=255)
                                                                                                                                         gCAAGCGCCGAGCTTGCCGGCAGGAACGGGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGttt          <  2:1710542/150‑1 (MQ=255)
                                                                                                                                          cAAGCGCCGAGCTTGCCGTCATGAACGTGCTTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGCTCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTg         <  1:170668/150‑1 (MQ=255)
                                                                                                                                          cAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTATAATCGGGTTGAACTTTTTCTCGCGGGCGTTTg         >  2:1551715/1‑150 (MQ=255)
                                                                                                                                          cAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTg         >  1:1574798/1‑150 (MQ=255)
                                                                                                                                          cAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTg         >  1:1254444/1‑150 (MQ=255)
                                                                                                                                          cAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTg         <  2:433754/150‑1 (MQ=255)
                                                                                                                                           aaGCGCCGAGCTTGCCGTCAGGAACGTGCGGCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTga        <  1:1836224/150‑1 (MQ=255)
                                                                                                                                            aGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTga        <  2:1018014/150‑1 (MQ=255)
                                                                                                                                            aGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTgac       >  1:1533183/1‑150 (MQ=255)
                                                                                                                                             gcgcCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGAGTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTgacg      >  1:1002619/1‑150 (MQ=255)
                                                                                                                                              cgcCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTgacgc     >  2:813038/1‑149 (MQ=255)
                                                                                                                                                 cGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTGACGACCg  <  2:673469/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
ATCCGGCAGGCACGACGACTGTTTGCCCTTCGCTGAAGGCTTGTTTAAATGAGGCGATCCAGTCGTGTGGGTTGTAGTCGTTAATGTTAACGCTTTGTCGGGCGGGAAGCGCGCGGGCGAAAGGGGTATGGAGGAAGGCAAGCGCCGAGCTTGCCGTCAGGAACGTGCGTCGGGAGAGTTTTTTAAATGGCATGCGTTCTCCTCTATAAAGCCTGCAGCAAGCTGGCGAGTTCTCGATTGATCACCTGCTGGTTAAAATCGTGTTCAACTTTTTCGCGCGCGCGTTTGACGACCG  >  NC_000913/2115704‑2115998

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: