Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,171,814 A→G 5.1% intergenic (‑87/+19) gatR ← / ← gatD pseudogene, repressor for gat operon; interrupted by IS3; split galactitol utilization operon repressor, fragment 2; split galactitol utilization operon repressor, interrupted/galactitol‑1‑phosphate dehydrogenase, Zn‑dependent and NAD(P)‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,171,8140AG 5.1% 346.9 / 6.9 118intergenic (‑87/+19)gatR/gatDpseudogene, repressor for gat operon; interrupted by IS3; split galactitol utilization operon repressor, fragment 2; split galactitol utilization operon repressor, interrupted/galactitol‑1‑phosphate dehydrogenase, Zn‑dependent and NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base A (66/45);  new base G (3/3);  total (69/49)
Fisher's exact test for biased strand distribution p-value = 6.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.09e-01

Only 100 of 120 total aligned reads displayed.

AGCACGGTTCCCTGTTCATTCACTAATTGGATGATCTTATTCCTTCGCTCGAATGAATTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACTTACGTTCTGTCAGCAACC  >  NC_000913/2171666‑2171963
                                                                                                                                                     |                                                                                                                                                      
aGCACGGTTCCCTGTTCATTCACTAATTGGATGATCTTATTCCTTCGCTCGAATGAATTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCAGTTTTGCACAATACCGGGCCAGCAt                                                                                                                                                       >  2:1255886/1‑150 (MQ=255)
  cACGGTTCCCTGTTCATTCACTAATTGGATGATCTTATTCCTTCGCTCGAATGAATTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATcc                                                                                                                                                     >  2:1882400/1‑149 (MQ=255)
      gTTCCCTGTTCATTCACTAATTGGATGATCTTATTCCTTCGCTCGAATGAATTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTAGCACAATACCGGGCCAGCATCACGCt                                                                                                                                                 >  2:1411628/1‑150 (MQ=255)
           cTGTTCATTCACTAATTGGATGATCTTATTCCTTCGCTCGAATGAATTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGccc                                                                                                                                            >  1:891436/1‑150 (MQ=255)
           cTGTTCATTCACTAATTGGATGATCTTATTCCTTCGCTCGAATGAATTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAAAGATCTGTTTTGCACAAAACAGGGCCAGCATCACGCTGGccc                                                                                                                                            >  2:1407947/1‑150 (MQ=255)
            tGTTCATTCACTAATTGGATGATCTTATTCCTTCGCTCGAATGAATTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCGTGATGCTGGCCcg                                                                                                                                           <  2:1066086/150‑1 (MQ=255)
             tttcattcaCTAATTGGATGAGCTTATTCCTTCGCTCGAATGAAGTCATCGTCATTTACGCATTTTCAATATTGGCTAGTGTATACGAAGGGGGACCATTGGTGAATGAGCTGTTTTGCACAATACCGGGCCAGCGTGATGCTGGCCcgg                                                                                                                                          <  1:93882/149‑2 (MQ=255)
             gTTCATTCACTAATTGGATGATCTTATTCCTTCGCTCGAATGAATTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCGTGATGCTGGCCcgg                                                                                                                                          <  2:78604/150‑2 (MQ=255)
              tgcattcaCTAATTGGATGATCTTATTCCTTCTCTCGAATGAATTCATCGTCATTTTCCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCcgcg                                                                                                                                         <  2:1148216/148‑1 (MQ=255)
                  ttcaCTAATTGGATGATCTTATTCCTTCGCTCGAATGAATTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGttt                                                                                                                                     >  1:389810/1‑150 (MQ=255)
                  ttcaCTAATTGGATGATCTTATTCCTTCGCTCGAATGAATTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGttt                                                                                                                                     <  1:691559/150‑1 (MQ=255)
                     aCTAATTGGATGATCTTATTCCTTCGCTCGAATGAATTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAg                                                                                                                                  >  2:1010318/1‑150 (MQ=255)
                      cTAATTGGATGATCTTATTCCTTCGCTCGAATGAATTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAgg                                                                                                                                 <  1:241749/150‑1 (MQ=255)
                       tAATTGGATGATCTTATTCCTTCGCTCGAATGAATTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAggg                                                                                                                                <  2:579948/150‑1 (MQ=255)
                       tAATTGGATGATCTTATTCCTTCGCTCGAATGAATTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAggg                                                                                                                                <  1:1075275/150‑1 (MQ=255)
                           tGGATGATCTTATTCCTTCGCTCGAATGAATTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATg                                                                                                                            <  2:1529425/150‑1 (MQ=255)
                           tGGATGATCTTATTCCTTCGCTCGAATGAATTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATg                                                                                                                            >  1:222138/1‑150 (MQ=255)
                             gatgctCTTATTCCTTCGCTCGAATGAATTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTACACGAAGGGGGACAATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATgcc                                                                                                                          >  2:981963/1‑148 (MQ=255)
                              atgatCTTATTCCTTCGCTCGAATGAATTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGc                                                                                                                         >  2:598248/1‑150 (MQ=255)
                              atgatCTAATTCCTTCGCTCGAATCAATTCATCGTCATTTACCCATATTCAATTGTGGCTCGTGTAAACGAAGGGGGACCATTGGGGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGc                                                                                                                         <  1:933318/150‑1 (MQ=255)
                               tgatCTTATTCCTTCGCTCGAATGAATTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCa                                                                                                                        >  2:996719/1‑150 (MQ=255)
                                    ttATTCCTTCGCTCGAATGAATTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACt                                                                                                                  <  2:205240/150‑1 (MQ=255)
                                     tATTCCTTCGCTCGAATGAATTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACtt                                                                                                                 >  1:882539/1‑150 (MQ=255)
                                      aTTCCTTCGCTCGAATGAATTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACttt                                                                                                                >  2:1092844/1‑150 (MQ=255)
                                        tCCTTCGCTCGAATGAATTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGc                                                                                                              >  2:107152/1‑150 (MQ=255)
                                                tCGAATGAATTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATa                                                                                                      >  1:514219/1‑150 (MQ=255)
                                                 ggaaggaatTCATCGTCTTTTACCAATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATTATCTGTTTTGCACAATACCGGGCCAGCATCACGCTTGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAg                                                                                                     <  2:443756/149‑1 (MQ=255)
                                                 cGAATGAATTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAg                                                                                                     >  1:1356051/1‑150 (MQ=255)
                                                 cGAATGAATTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAg                                                                                                     >  2:42682/1‑150 (MQ=255)
                                                  caatgaatTCATCGTCATTAACCCATATTCATTTGTGGCTAGTGTAAACGACGGGGCACCATTGGTTAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCGGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGc                                                                                                    <  1:624073/149‑1 (MQ=255)
                                                   aatgaatTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCCGCATCACCCTGGCGCGCGGTTTCAGGGAAAGCGCAACCAATTTGCCGGCATAgac                                                                                                   >  1:1708384/1‑148 (MQ=255)
                                                   aatgaatTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCa                                                                                                   >  2:1545521/1‑150 (MQ=255)
                                                   aatgaatTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGAACAAAACCGGGCCAGAAACACGCTTGCCCGCGGTTTCATGGAATGAGCAACACTTTGCCCGGCATAgaa                                                                                                   >  1:1702047/1‑148 (MQ=255)
                                                    atgaatTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCAt                                                                                                  <  2:56206/150‑1 (MQ=255)
                                                        atTCATCGTCATTTACCCATCTTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACg                                                                                              <  2:1112651/150‑1 (MQ=255)
                                                          tCATCGTCATTTACCAATATTCATTTGTGGCTTGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAg                                                                                            <  2:621354/150‑1 (MQ=255)
                                                            aTCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCAGCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCg                                                                                          <  1:497510/150‑1 (MQ=255)
                                                              cGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAAAACTTTGCACGGCATAGCATTACGAGCGAt                                                                                        >  1:788823/1‑150 (MQ=255)
                                                               gTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATg                                                                                       >  2:154556/1‑150 (MQ=255)
                                                                 cATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTc                                                                                     >  2:1061940/1‑150 (MQ=255)
                                                                 cATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGAAGTc                                                                                     >  2:470369/1‑150 (MQ=255)
                                                                   tttACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCAc                                                                                   <  1:1337417/150‑1 (MQ=255)
                                                                       cccATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTGTAAGGGAATGAGCAACCCTTTGCCCGGCATAGCATTACGAGCGATGgcacgcac                                                                               >  2:1414377/1‑150 (MQ=255)
                                                                        ccATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCACGCGGTTTCAGGGTATGAGCAACACTTTGCCCGGCATCGCATTACGAGCGATGTCACGCAcc                                                                              >  1:298381/1‑150 (MQ=255)
                                                                         cATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCg                                                                             >  2:1682751/1‑150 (MQ=255)
                                                                           ttttCAATTTTGGGTAGTGTAAACGAAGGGGTACCATTGGTGAATGATCTGTTTTGCACAATACCGGCCCAGCATCACGCGGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGcc                                                                           <  1:1532688/148‑1 (MQ=255)
                                                                           tatTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGcc                                                                           <  1:227275/150‑1 (MQ=255)
                                                                             ttCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTTGTGAATGATCTGTTTTGCAAAATACCGGGCCAGCATCACTCTGGCCCGCGGTTTCAGGTAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTg                                                                         <  2:1260483/150‑1 (MQ=255)
                                                                              tCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCCCGCACCGCCTgg                                                                        >  2:582666/1‑150 (MQ=255)
                                                                               cAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGCGCAACACTTTGCCCGGCCTAGCATTACGCGCGATGTCACGCACCGCCTggg                                                                       >  2:324037/1‑150 (MQ=255)
                                                                               cAATTGTGGCAAGGGTAAACGAAGGGGGCCCATGGGGGAATGATCGGTTTTGCACAATACCGGGCCAGCATCACGCGGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTggg                                                                       <  1:340065/150‑1 (MQ=255)
                                                                                aaTTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGc                                                                      >  1:1767746/1‑150 (MQ=255)
                                                                                   tgaggCTAGTGTAAACGAAGGGGGACCATTGCTGAATGAACTGATTTTCTCAATACCGGGCAAGCATACCGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTATCCCCGCCAAACCAATACGAGCGATGTCACGCCCCGCCTGGGCGaa                                                                   >  1:907292/4‑150 (MQ=255)
                                                                                    gtgGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAg                                                                  >  1:950300/1‑150 (MQ=255)
                                                                                      ggCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCCGCATCACGCTGGCCCGCGGTTTCAGGGAATGCGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGCAGCt                                                                >  2:1368896/1‑150 (MQ=255)
                                                                                      ggCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCCCGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCCCCGCCTGCGCGCAGCt                                                                >  2:307713/1‑150 (MQ=255)
                                                                                          attgtAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGATTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCa                                                            <  1:1027475/148‑1 (MQ=255)
                                                                                          aGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCa                                                            <  1:107152/150‑1 (MQ=255)
                                                                                           gtgtAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCATTCaa                                                           >  2:1383948/1‑150 (MQ=255)
                                                                                            tgtAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCaaa                                                          >  1:1251547/1‑150 (MQ=255)
                                                                                             gtAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAg                                                         <  2:1369792/150‑1 (MQ=255)
                                                                                              tAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGc                                                        >  2:648989/1‑150 (MQ=255)
                                                                                                aaCGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCtt                                                      >  1:217840/1‑150 (MQ=255)
                                                                                                    aaGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCAc                                                  >  2:779882/1‑150 (MQ=255)
                                                                                                       ggggACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGt                                               <  2:172738/150‑1 (MQ=255)
                                                                                                           cccATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCGGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGc                                           <  2:419581/149‑1 (MQ=255)
                                                                                                           aCCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGc                                           <  2:1009040/150‑1 (MQ=255)
                                                                                                            ccATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCg                                          >  1:1360402/1‑150 (MQ=255)
                                                                                                             cATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGa                                         >  2:1218494/1‑150 (MQ=255)
                                                                                                               ttGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGAtt                                       <  1:154556/150‑1 (MQ=255)
                                                                                                                   tGAATGTTGGGTTTTGCAAAATACGGGTCATCTATCTGGGTGGCCCTCTGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTAAGATCGATGTTACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATg                                   <  2:1209118/150‑1 (MQ=255)
                                                                                                                    gAATGATCTGTTTTGCACAATACCGGGCCAGCATGACGCAGGCCCGCGGTTTCAGGGAATGACCAACACTTTGCCCGGCATAGCATTACCAGCGATGTCACGCACCGCCTTGGCGAACCTTTCTAACCTGCCACGGTGAGCGATTAATgg                                  >  2:471320/1‑150 (MQ=255)
                                                                                                                     aaTGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTATCCCGGCATAGCATTACGAGCGATGACACGAACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGc                                 >  2:1774222/1‑150 (MQ=255)
                                                                                                                      aTGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCt                                >  1:424501/1‑150 (MQ=255)
                                                                                                                       tGATCTGTTTTGCACAATACCGGGCCAGCATCCCGCTGGCCCGCGGTTTCAGGGAATGAGCAGCACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTc                               >  2:1176598/1‑150 (MQ=255)
                                                                                                                       tGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTc                               >  2:957401/1‑150 (MQ=255)
                                                                                                                        gATCTGTTTTGCACAACACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTcc                              >  1:914298/1‑150 (MQ=255)
                                                                                                                         aTCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCa                             >  2:127595/1‑150 (MQ=255)
                                                                                                                         aTCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGGTTGAGGGAATTGGCAACACGTTGACCGGAATAGGTTTTAGCGCGATGTAACGCACCGCCTGGGCGCAGGTTTCAAAGCTTGCCCGGTGAGGGGTTAAgggggccc                             >  2:1725080/1‑145 (MQ=255)
                                                                                                                          tCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAg                            <  2:950300/150‑1 (MQ=255)
                                                                                                                             gTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCt                         >  1:529618/1‑150 (MQ=255)
                                                                                                                              ttttGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCtt                        <  1:803599/150‑1 (MQ=255)
                                                                                                                               tttGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCGTTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTa                       >  1:1449806/1‑150 (MQ=255)
                                                                                                                                  gCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGTCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACt                    <  1:763856/150‑1 (MQ=255)
                                                                                                                                  gCACAATACCGGGCCAGCATCACGCTGGCCCGCGGGTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTTCGAGCGATGTCCCGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATGATTGGCTCCAGGCTTAACt                    >  2:689865/1‑150 (MQ=255)
                                                                                                                                     caATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACTTAc                 >  1:1316395/1‑150 (MQ=255)
                                                                                                                                     caATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACGTAc                 >  1:139103/1‑150 (MQ=255)
                                                                                                                                      aaTCCCGGGCCAGCATCCCGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACTTACg                <  1:1413017/150‑1 (MQ=255)
                                                                                                                                       aTACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGCAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACTTACGt               >  2:269356/1‑150 (MQ=255)
                                                                                                                                        ccgcGGGCCAGCGTGATGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGCTGTCCCGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGCTTAATGGCTCCAGGCTTAACTTACGtt              >  1:1128408/4‑150 (MQ=255)
                                                                                                                                          gcGGGCCAGCGTGATGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCGTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACTTACGTTCt            >  1:339654/2‑150 (MQ=255)
                                                                                                                                          ccGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACTTACGTTCt            >  1:1717208/1‑150 (MQ=255)
                                                                                                                                           cGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTGTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACTTACGTTCTg           <  2:976384/150‑1 (MQ=255)
                                                                                                                                              gCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGATTCCACGGTGAGCGATTAATGGCTCCAGGCTTACCTTACGTTCTGTCa        >  2:251440/1‑150 (MQ=255)
                                                                                                                                              ccccgcagcaCGCGGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCGCCAGGCTTAACTTACGTTCTGTCa        <  2:1171162/146‑1 (MQ=255)
                                                                                                                                               ccAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACTTACGTTCTGTCAg       <  2:650333/150‑1 (MQ=255)
                                                                                                                                               ccAGCATCACGCTGGCCCGCGGGTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACTTACGTTCTGTCAg       <  1:1692033/150‑1 (MQ=255)
                                                                                                                                                cAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTTAGCGATTAATGGCTCCAGGCTTAACTTACGTTCTGTCAGc      <  2:320232/150‑1 (MQ=255)
                                                                                                                                                   cATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCACAGCATCCACGGTGAGCGATTAATGGCTCCAGGCTTAACTTACGGTCTGTCAGCAAc   >  1:1679695/1‑150 (MQ=255)
                                                                                                                                                     aTCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACTTACGTTCTGTCAGCAAcc  <  1:1010318/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
AGCACGGTTCCCTGTTCATTCACTAATTGGATGATCTTATTCCTTCGCTCGAATGAATTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACTTACGTTCTGTCAGCAACC  >  NC_000913/2171666‑2171963

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: