Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,171,816 C→G 5.9% intergenic (‑89/+17) gatR ← / ← gatD pseudogene, repressor for gat operon; interrupted by IS3; split galactitol utilization operon repressor, fragment 2; split galactitol utilization operon repressor, interrupted/galactitol‑1‑phosphate dehydrogenase, Zn‑dependent and NAD(P)‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,171,8160CG 5.9% 342.4 / 10.7 116intergenic (‑89/+17)gatR/gatDpseudogene, repressor for gat operon; interrupted by IS3; split galactitol utilization operon repressor, fragment 2; split galactitol utilization operon repressor, interrupted/galactitol‑1‑phosphate dehydrogenase, Zn‑dependent and NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base C (62/46);  new base G (4/3);  total (67/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.81e-01

Only 100 of 121 total aligned reads displayed.

GCACGGTTCCCTGTTCATTCACTAATTGGATGATCTTATTCCTTCGCTCGAATGAATTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACTTACGTTCTGTCAGCAACCGG  >  NC_000913/2171667‑2171965
                                                                                                                                                     |                                                                                                                                                      
gCACGGTTCCCTGTTCATTCACTAATTGGATGATCTTATTCCTTCGATCGTATGAATTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATc                                                                                                                                                        <  1:258624/150‑1 (MQ=255)
 cACGGTTCCCTGTTCATTCACTAATTGGATGATCTTATTCCTTCGCTCGAATGAATTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATcc                                                                                                                                                       >  2:1882400/1‑149 (MQ=255)
     gTTCCCTGTTCATTCACTAATTGGATGATCTTATTCCTTCGCTCGAATGAATTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTAGCACAATACCGGGCCAGCATCACGCt                                                                                                                                                   >  2:1411628/1‑150 (MQ=255)
          cTGTTCATTCACTAATTGGATGATCTTATTCCTTCGCTCGAATGAATTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGccc                                                                                                                                              >  1:891436/1‑150 (MQ=255)
          cTGTTCATTCACTAATTGGATGATCTTATTCCTTCGCTCGAATGAATTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAAAGATCTGTTTTGCACAAAACAGGGCCAGCATCACGCTGGccc                                                                                                                                              >  2:1407947/1‑150 (MQ=255)
           tGTTCATTCACTAATTGGATGATCTTATTCCTTCGCTCGAATGAATTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCGTGATGCTGGCCcg                                                                                                                                             <  2:1066086/150‑1 (MQ=255)
            tttcattcaCTAATTGGATGAGCTTATTCCTTCGCTCGAATGAAGTCATCGTCATTTACGCATTTTCAATATTGGCTAGTGTATACGAAGGGGGACCATTGGTGAATGAGCTGTTTTGCACAATACCGGGCCAGCGTGATGCTGGCCcgg                                                                                                                                            <  1:93882/149‑2 (MQ=255)
            gTTCATTCACTAATTGGATGATCTTATTCCTTCGCTCGAATGAATTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCGTGATGCTGGCCcgg                                                                                                                                            <  2:78604/150‑2 (MQ=255)
             tgcattcaCTAATTGGATGATCTTATTCCTTCTCTCGAATGAATTCATCGTCATTTTCCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCcgcg                                                                                                                                           <  2:1148216/148‑1 (MQ=255)
              tcattcaCTAATGGGATGATCTTATTCCTTCGCGAGAATGAATTCATCGCCATTTACCCATATTCAATTGTGGCTCGGGTAAACTACGGGGGACCATTGGTTAATTATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCgg                                                                                                                                          <  2:829672/150‑1 (MQ=255)
                 ttcaCTAATTGGATGATCTTATTCCTTCGCTCGAATGAATTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGttt                                                                                                                                       <  1:691559/150‑1 (MQ=255)
                     cTAATTGGATGATCTTATTCCTTCGCTCGAATGAATTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAgg                                                                                                                                   <  1:241749/150‑1 (MQ=255)
                      tAATTGGATGATCTTATTCCTTCGCTCGAATGAATTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAggg                                                                                                                                  <  1:1075275/150‑1 (MQ=255)
                      tAATTGGATGATCTTATTCCTTCGCTCGAATGAATTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAggg                                                                                                                                  <  2:579948/150‑1 (MQ=255)
                          tGGATGATCTTATTCCTTCGCTCGAATGAATTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATg                                                                                                                              >  1:222138/1‑150 (MQ=255)
                            gatgctCTTATTCCTTCGCTCGAATGAATTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTACACGAAGGGGGACAATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATgcc                                                                                                                            >  2:981963/1‑148 (MQ=255)
                             atgatCTTATTCCTTCGCTCGAATGAATTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGc                                                                                                                           >  2:598248/1‑150 (MQ=255)
                              tgatCTTATTCCTTCGCTCGAATGAATTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCa                                                                                                                          >  2:996719/1‑150 (MQ=255)
                                  cTTATTCCTTCGCTCGAATGAATTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAacac                                                                                                                     >  2:1709169/1‑150 (MQ=255)
                                   ttATTCCTTCGCTCGAATGAATTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACt                                                                                                                    <  2:205240/150‑1 (MQ=255)
                                       tCCTTCGCTCGAATGAATTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGc                                                                                                                >  2:107152/1‑150 (MQ=255)
                                               tCGAATGAATTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATa                                                                                                        >  1:514219/1‑150 (MQ=255)
                                                ggaaggaatTCATCGTCTTTTACCAATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATTATCTGTTTTGCACAATACCGGGCCAGCATCACGCTTGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAg                                                                                                       <  2:443756/149‑1 (MQ=255)
                                                cGAATGAATTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAg                                                                                                       >  2:42682/1‑150 (MQ=255)
                                                cGAATGAATTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAg                                                                                                       >  1:1356051/1‑150 (MQ=255)
                                                  aatgaatTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCCGCATCACCCTGGCGCGCGGTTTCAGGGAAAGCGCAACCAATTTGCCGGCATAgac                                                                                                     >  1:1708384/1‑148 (MQ=255)
                                                  aatgaatTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCa                                                                                                     >  2:1545521/1‑150 (MQ=255)
                                                  aatgaatTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGAACAAAACCGGGCCAGAAACACGCTTGCCCGCGGTTTCATGGAATGAGCAACACTTTGCCCGGCATAgaa                                                                                                     >  1:1702047/1‑148 (MQ=255)
                                                       atTCATCGTCATTTACCCATCTTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACg                                                                                                <  2:1112651/150‑1 (MQ=255)
                                                       atTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACg                                                                                                >  1:172738/1‑150 (MQ=255)
                                                         tCATCGTCATTTACCAATATTCATTTGTGGCTTGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAg                                                                                              <  2:621354/150‑1 (MQ=255)
                                                          cATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGc                                                                                             >  1:479286/1‑150 (MQ=255)
                                                           aTCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCAGCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCg                                                                                            <  1:497510/150‑1 (MQ=255)
                                                             cGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAAAACTTTGCACGGCATAGCATTACGAGCGAt                                                                                          >  1:788823/1‑150 (MQ=255)
                                                              gTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATg                                                                                         >  2:154556/1‑150 (MQ=255)
                                                               tCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGt                                                                                        <  2:37241/150‑1 (MQ=255)
                                                                cATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTc                                                                                       >  2:1061940/1‑150 (MQ=255)
                                                                cATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGAAGTc                                                                                       >  2:470369/1‑150 (MQ=255)
                                                                  tttACCTATATTCATTTTTGGATTTTTTAAACTTAGGGGTACCTTTGGTTAATGTTCTGTTTTGCACATTACCGGGCCAGCTCCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCAc                                                                                     <  2:639511/150‑1 (MQ=255)
                                                                  tttACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCAc                                                                                     <  1:1337417/150‑1 (MQ=255)
                                                                      cccATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTGTAAGGGAATGAGCAACCCTTTGCCCGGCATAGCATTACGAGCGATGgcacgcac                                                                                 >  2:1414377/1‑150 (MQ=255)
                                                                       ccATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCACGCGGTTTCAGGGTATGAGCAACACTTTGCCCGGCATCGCATTACGAGCGATGTCACGCAcc                                                                                >  1:298381/1‑150 (MQ=255)
                                                                        cATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCg                                                                               >  2:1682751/1‑150 (MQ=255)
                                                                          ttttCAATTTTGGGTAGTGTAAACGAAGGGGTACCATTGGTGAATGATCTGTTTTGCACAATACCGGCCCAGCATCACGCGGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGcc                                                                             <  1:1532688/148‑1 (MQ=255)
                                                                            ttCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTTGTGAATGATCTGTTTTGCAAAATACCGGGCCAGCATCACTCTGGCCCGCGGTTTCAGGTAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTg                                                                           <  2:1260483/150‑1 (MQ=255)
                                                                             tCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCCCGCACCGCCTgg                                                                          >  2:582666/1‑150 (MQ=255)
                                                                              cAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGCGCAACACTTTGCCCGGCCTAGCATTACGCGCGATGTCACGCACCGCCTggg                                                                         >  2:324037/1‑150 (MQ=255)
                                                                              cAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCTACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTggg                                                                         <  2:1308649/150‑1 (MQ=255)
                                                                              cAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTggg                                                                         >  2:1071902/1‑150 (MQ=255)
                                                                              cAATTGTGGCAAGGGTAAACGAAGGGGGCCCATGGGGGAATGATCGGTTTTGCACAATACCGGGCCAGCATCACGCGGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTggg                                                                         <  1:340065/150‑1 (MQ=255)
                                                                               aaTTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGc                                                                        >  1:1767746/1‑150 (MQ=255)
                                                                                  tgaggCTAGTGTAAACGAAGGGGGACCATTGCTGAATGAACTGATTTTCTCAATACCGGGCAAGCATACCGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTATCCCCGCCAAACCAATACGAGCGATGTCACGCCCCGCCTGGGCGaa                                                                     >  1:907292/4‑150 (MQ=255)
                                                                                   gtgGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAg                                                                    >  1:950300/1‑150 (MQ=255)
                                                                                     ggCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCCCGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCCCCGCCTGCGCGCAGCt                                                                  >  2:307713/1‑150 (MQ=255)
                                                                                      gCTCGTGTAAACGAAGGGGGACCATTGGGGAATGAGCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCtt                                                                 <  2:482584/150‑1 (MQ=255)
                                                                                         attgtAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGATTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCa                                                              <  1:1027475/148‑1 (MQ=255)
                                                                                         aGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCa                                                              <  1:107152/150‑1 (MQ=255)
                                                                                          gtgtAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCATTCaa                                                             >  2:1383948/1‑150 (MQ=255)
                                                                                           tgtAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCaaa                                                            >  1:1251547/1‑150 (MQ=255)
                                                                                            gtAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAg                                                           <  2:1369792/150‑1 (MQ=255)
                                                                                              aaaCGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGAATTACGAGCGATTTCACGCACCGCCTGGGCGAAGCTTTCAAAGCt                                                         >  1:748072/1‑150 (MQ=255)
                                                                                               aaCGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCtt                                                        >  1:217840/1‑150 (MQ=255)
                                                                                                   aaGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCAc                                                    >  2:779882/1‑150 (MQ=255)
                                                                                                         gACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAg                                              <  2:1187552/150‑1 (MQ=255)
                                                                                                          aCCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGc                                             <  2:1009040/150‑1 (MQ=255)
                                                                                                           ccATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCg                                            >  1:1360402/1‑150 (MQ=255)
                                                                                                            cATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGa                                           >  2:1218494/1‑150 (MQ=255)
                                                                                                              ttGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGAtt                                         <  1:154556/150‑1 (MQ=255)
                                                                                                                   gAATGATCTGTTTTGCACAATACCGGGCCAGCATGACGCAGGCCCGCGGTTTCAGGGAATGACCAACACTTTGCCCGGCATAGCATTACCAGCGATGTCACGCACCGCCTTGGCGAACCTTTCTAACCTGCCACGGTGAGCGATTAATgg                                    >  2:471320/1‑150 (MQ=255)
                                                                                                                    aaTGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTATCCCGGCATAGCATTACGAGCGATGACACGAACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGc                                   >  2:1774222/1‑150 (MQ=255)
                                                                                                                     aTGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCt                                  >  1:424501/1‑150 (MQ=255)
                                                                                                                      tGATCTGTTTTGCACAATACCGGGCCAGCATCCCGCTGGCCCGCGGTTTCAGGGAATGAGCAGCACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTc                                 >  2:1176598/1‑150 (MQ=255)
                                                                                                                      tGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTc                                 >  2:957401/1‑150 (MQ=255)
                                                                                                                       gATCTGTTTTGCACAACACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTcc                                >  1:914298/1‑150 (MQ=255)
                                                                                                                        aTCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCa                               >  2:127595/1‑150 (MQ=255)
                                                                                                                        aTCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGGTTGAGGGAATTGGCAACACGTTGACCGGAATAGGTTTTAGCGCGATGTAACGCACCGCCTGGGCGCAGGTTTCAAAGCTTGCCCGGTGAGGGGTTAAgggggccc                               >  2:1725080/1‑145 (MQ=255)
                                                                                                                            gTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCt                           >  1:529618/1‑150 (MQ=255)
                                                                                                                             ttttGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCtt                          <  1:803599/150‑1 (MQ=255)
                                                                                                                              tttGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCGTTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTa                         >  1:1449806/1‑150 (MQ=255)
                                                                                                                                 gCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGTCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACt                      <  1:763856/150‑1 (MQ=255)
                                                                                                                                 gCACAATACCGGGCCAGCATCACGCTGGCCCGCGGGTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTTCGAGCGATGTCCCGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATGATTGGCTCCAGGCTTAACt                      >  2:689865/1‑150 (MQ=255)
                                                                                                                                  cacaATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACtt                     <  2:222138/150‑1 (MQ=255)
                                                                                                                                    caATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACTTAc                   >  1:1316395/1‑150 (MQ=255)
                                                                                                                                    caATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACGTAc                   >  1:139103/1‑150 (MQ=255)
                                                                                                                                     aaTCCCGGGCCAGCATCCCGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACTTACg                  <  1:1413017/150‑1 (MQ=255)
                                                                                                                                       ccgcGGGCCAGCGTGATGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGCTGTCCCGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGCTTAATGGCTCCAGGCTTAACTTACGtt                >  1:1128408/4‑150 (MQ=255)
                                                                                                                                         gcGGGCCAGCGTGATGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCGTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACTTACGTTCt              >  1:339654/2‑150 (MQ=255)
                                                                                                                                          cGGGCCAGCGTGATGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGCGCGATTAATGGCTCCAGGCTTATCTTACGTTCTg             >  1:973577/1‑150 (MQ=255)
                                                                                                                                          cGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTGTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACTTACGTTCTg             <  2:976384/150‑1 (MQ=255)
                                                                                                                                             gCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACTTACGTTCTGTCa          <  1:894852/150‑1 (MQ=255)
                                                                                                                                             gCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGATTCCACGGTGAGCGATTAATGGCTCCAGGCTTACCTTACGTTCTGTCa          >  2:251440/1‑150 (MQ=255)
                                                                                                                                             ccccgcagcaCGCGGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCGCCAGGCTTAACTTACGTTCTGTCa          <  2:1171162/146‑1 (MQ=255)
                                                                                                                                              ccAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACTTACGTTCTGTCAg         <  2:650333/150‑1 (MQ=255)
                                                                                                                                              ccAGCATCACGCTGGCCCGCGGGTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACTTACGTTCTGTCAg         <  1:1692033/150‑1 (MQ=255)
                                                                                                                                               cAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTTAGCGATTAATGGCTCCAGGCTTAACTTACGTTCTGTCAGc        <  2:320232/150‑1 (MQ=255)
                                                                                                                                                  cATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCACAGCATCCACGGTGAGCGATTAATGGCTCCAGGCTTAACTTACGGTCTGTCAGCAAc     >  1:1679695/1‑150 (MQ=255)
                                                                                                                                                   aTCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACTTACGTTCTGTCAGCAAcc    <  1:1010318/150‑1 (MQ=255)
                                                                                                                                                    tCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACTTACGTTCTGTCAGCAACCg   >  1:1609709/1‑150 (MQ=255)
                                                                                                                                                     cACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACTTACGTTCTGTCAGCAACCgg  >  2:1014503/1‑150 (MQ=255)
                                                                                                                                                     cACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACTTACGTTCTGTCAGCAACCgg  >  2:1007932/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
GCACGGTTCCCTGTTCATTCACTAATTGGATGATCTTATTCCTTCGCTCGAATGAATTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACTTACGTTCTGTCAGCAACCGG  >  NC_000913/2171667‑2171965

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: