Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,171,818 C→T 5.1% intergenic (‑91/+15) gatR ← / ← gatD pseudogene, repressor for gat operon; interrupted by IS3; split galactitol utilization operon repressor, fragment 2; split galactitol utilization operon repressor, interrupted/galactitol‑1‑phosphate dehydrogenase, Zn‑dependent and NAD(P)‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,171,8180CT 5.1% 364.1 / 4.8 119intergenic (‑91/+15)gatR/gatDpseudogene, repressor for gat operon; interrupted by IS3; split galactitol utilization operon repressor, fragment 2; split galactitol utilization operon repressor, interrupted/galactitol‑1‑phosphate dehydrogenase, Zn‑dependent and NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base C (66/46);  new base T (3/3);  total (69/50)
Fisher's exact test for biased strand distribution p-value = 6.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.33e-01

Only 100 of 119 total aligned reads displayed.

GTTCCCTGTTCATTCACTAATTGGATGATCTTATTCCTTCGCTCGAATGAATTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACTTACGTTCTGTCAGCAACCGG  >  NC_000913/2171672‑2171965
                                                                                                                                                  |                                                                                                                                                    
gTTCCCTGTTCATTCACTAATTGGATGATCTTATTCCTTCGCTCGAATGAATTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTAGCACAATACCGGGCCAGCATCACGCt                                                                                                                                                   >  2:1411628/1‑150 (MQ=255)
     cTGTTCATTCACTAATTGGATGATCTTATTCCTTCGCTCGAATGAATTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGccc                                                                                                                                              >  1:891436/1‑150 (MQ=255)
     cTGTTCATTCACTAATTGGATGATCTTATTCCTTCGCTCGAATGAATTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAAAGATCTGTTTTGCACAAAACAGGGCCAGCATCACGCTGGccc                                                                                                                                              >  2:1407947/1‑150 (MQ=255)
      tGTTCATTCACTAATTGGATGATCTTATTCCTTCGCTCGAATGAATTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCGTGATGCTGGCCcg                                                                                                                                             <  2:1066086/150‑1 (MQ=255)
       tttcattcaCTAATTGGATGAGCTTATTCCTTCGCTCGAATGAAGTCATCGTCATTTACGCATTTTCAATATTGGCTAGTGTATACGAAGGGGGACCATTGGTGAATGAGCTGTTTTGCACAATACCGGGCCAGCGTGATGCTGGCCcgg                                                                                                                                            <  1:93882/149‑2 (MQ=255)
       gTTCATTCACTAATTGGATGATCTTATTCCTTCGCTCGAATGAATTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCGTGATGCTGGCCcgg                                                                                                                                            <  2:78604/150‑2 (MQ=255)
        tgcattcaCTAATTGGATGATCTTATTCCTTCTCTCGAATGAATTCATCGTCATTTTCCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCcgcg                                                                                                                                           <  2:1148216/148‑1 (MQ=255)
            ttcaCTAATTGGATGATCTTATTCCTTCGCTCGAATGAATTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGttt                                                                                                                                       >  1:389810/1‑150 (MQ=255)
            ttcaCTAATTGGATGATCTTATTCCTTCGCTCGAATGAATTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGttt                                                                                                                                       <  1:691559/150‑1 (MQ=255)
                cTAATTGGATGATCTTATTCCTTCGCTCGAATGAATTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAgg                                                                                                                                   <  1:241749/150‑1 (MQ=255)
                 tAATTGGATGATCTTATTCCTTCGCTCGAATGAATTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAggg                                                                                                                                  <  1:1075275/150‑1 (MQ=255)
                 tAATTGGATGATCTTATTCCTTCGCTCGAATGAATTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAggg                                                                                                                                  <  2:579948/150‑1 (MQ=255)
                     tGGATGATCTTATTCCTTCGCTCGAATGAATTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATg                                                                                                                              >  1:222138/1‑150 (MQ=255)
                     tGGATGATCTTATTCCTTCGCTCGAATGAATTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATg                                                                                                                              <  2:1529425/150‑1 (MQ=255)
                       gatgctCTTATTCCTTCGCTCGAATGAATTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTACACGAAGGGGGACAATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATgcc                                                                                                                            >  2:981963/1‑148 (MQ=255)
                        atgatCTTATTCCTTCGCTCGAATGAATTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGc                                                                                                                           >  2:598248/1‑150 (MQ=255)
                         tgatCTTATTCCTTCGCTCGAATGAATTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCa                                                                                                                          >  2:996719/1‑150 (MQ=255)
                             cTTATTCCTTCGCTCGAATGAATTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAacac                                                                                                                     >  2:1709169/1‑150 (MQ=255)
                               tATTCCTTCGCTCGAATGAATTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACtt                                                                                                                   >  1:882539/1‑150 (MQ=255)
                                  tCCTTCGCTCGAATGAATTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGc                                                                                                                >  2:107152/1‑150 (MQ=255)
                                           ggaaggaatTCATCGTCTTTTACCAATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATTATCTGTTTTGCACAATACCGGGCCAGCATCACGCTTGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAg                                                                                                       <  2:443756/149‑1 (MQ=255)
                                           cGAATGAATTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAg                                                                                                       >  1:1356051/1‑150 (MQ=255)
                                           cGAATGAATTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAg                                                                                                       >  2:42682/1‑150 (MQ=255)
                                            caatgaatTCATCGTCATTAACCCATATTCATTTGTGGCTAGTGTAAACGACGGGGCACCATTGGTTAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCGGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGc                                                                                                      <  1:624073/149‑1 (MQ=255)
                                             aatgaatTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCCGCATCACCCTGGCGCGCGGTTTCAGGGAAAGCGCAACCAATTTGCCGGCATAgac                                                                                                     >  1:1708384/1‑148 (MQ=255)
                                             aatgaatTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCa                                                                                                     >  2:1545521/1‑150 (MQ=255)
                                             aatgaatTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGAACAAAACCGGGCCAGAAACACGCTTGCCCGCGGTTTCATGGAATGAGCAACACTTTGCCCGGCATAgaa                                                                                                     >  1:1702047/1‑148 (MQ=255)
                                              atgaatTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCAt                                                                                                    <  2:56206/150‑1 (MQ=255)
                                                  atTCATCGTCATTTACCCATCTTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACg                                                                                                <  2:1112651/150‑1 (MQ=255)
                                                  atTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACg                                                                                                >  1:172738/1‑150 (MQ=255)
                                                    tCATCGTCATTTACCAATATTCATTTGTGGCTTGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAg                                                                                              <  2:621354/150‑1 (MQ=255)
                                                     cATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGc                                                                                             >  1:479286/1‑150 (MQ=255)
                                                      aTCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCAGCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCg                                                                                            <  1:497510/150‑1 (MQ=255)
                                                          tCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGt                                                                                        <  2:37241/150‑1 (MQ=255)
                                                           cATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTc                                                                                       >  2:1061940/1‑150 (MQ=255)
                                                           cATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGAAGTc                                                                                       >  2:470369/1‑150 (MQ=255)
                                                             tttACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCAc                                                                                     <  1:1337417/150‑1 (MQ=255)
                                                                aCCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCa                                                                                  <  1:1130841/150‑1 (MQ=255)
                                                                 cccATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTGTAAGGGAATGAGCAACCCTTTGCCCGGCATAGCATTACGAGCGATGgcacgcac                                                                                 >  2:1414377/1‑150 (MQ=255)
                                                                  ccATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCACGCGGTTTCAGGGTATGAGCAACACTTTGCCCGGCATCGCATTACGAGCGATGTCACGCAcc                                                                                >  1:298381/1‑150 (MQ=255)
                                                                   cATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCg                                                                               >  2:1682751/1‑150 (MQ=255)
                                                                     ttttCAATTTTGGGTAGTGTAAACGAAGGGGTACCATTGGTGAATGATCTGTTTTGCACAATACCGGCCCAGCATCACGCGGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGcc                                                                             <  1:1532688/148‑1 (MQ=255)
                                                                     tatTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGcc                                                                             <  1:227275/150‑1 (MQ=255)
                                                                       ttCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTTGTGAATGATCTGTTTTGCAAAATACCGGGCCAGCATCACTCTGGCCCGCGGTTTCAGGTAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTg                                                                           <  2:1260483/150‑1 (MQ=255)
                                                                        tCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCCCGCACCGCCTgg                                                                          >  2:582666/1‑150 (MQ=255)
                                                                         cAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCTACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTggg                                                                         <  2:1308649/150‑1 (MQ=255)
                                                                         cAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTggg                                                                         >  2:1071902/1‑150 (MQ=255)
                                                                         cAATTGTGGCAAGGGTAAACGAAGGGGGCCCATGGGGGAATGATCGGTTTTGCACAATACCGGGCCAGCATCACGCGGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTggg                                                                         <  1:340065/150‑1 (MQ=255)
                                                                             tgaggCTAGTGTAAACGAAGGGGGACCATTGCTGAATGAACTGATTTTCTCAATACCGGGCAAGCATACCGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTATCCCCGCCAAACCAATACGAGCGATGTCACGCCCCGCCTGGGCGaa                                                                     >  1:907292/4‑150 (MQ=255)
                                                                              gtgGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAg                                                                    >  1:950300/1‑150 (MQ=255)
                                                                                ggCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCCGCATCACGCTGGCCCGCGGTTTCAGGGAATGCGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGCAGCt                                                                  >  2:1368896/1‑150 (MQ=255)
                                                                                ggCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCCCGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCCCCGCCTGCGCGCAGCt                                                                  >  2:307713/1‑150 (MQ=255)
                                                                                    attgtAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGATTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCa                                                              <  1:1027475/148‑1 (MQ=255)
                                                                                    aGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCa                                                              <  1:107152/150‑1 (MQ=255)
                                                                                       gtAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAg                                                           <  2:1369792/150‑1 (MQ=255)
                                                                                        tAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGc                                                          >  2:648989/1‑150 (MQ=255)
                                                                                         aaaCGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGAATTACGAGCGATTTCACGCACCGCCTGGGCGAAGCTTTCAAAGCt                                                         >  1:748072/1‑150 (MQ=255)
                                                                                          aaCGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCtt                                                        >  1:217840/1‑150 (MQ=255)
                                                                                              aaGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCAc                                                    >  2:779882/1‑150 (MQ=255)
                                                                                                 ggggACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGt                                                 <  2:172738/150‑1 (MQ=255)
                                                                                                    gACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAg                                              <  2:1187552/150‑1 (MQ=255)
                                                                                                     cccATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCGGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGc                                             <  2:419581/149‑1 (MQ=255)
                                                                                                     aCCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGc                                             <  2:1009040/150‑1 (MQ=255)
                                                                                                      ccATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCg                                            >  1:1360402/1‑150 (MQ=255)
                                                                                                       cATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGa                                           >  2:1218494/1‑150 (MQ=255)
                                                                                                         ttGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGAtt                                         <  1:154556/150‑1 (MQ=255)
                                                                                                          tGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCCCGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTa                                        >  1:1378293/1‑150 (MQ=255)
                                                                                                              gAATGATCTGTTTTGCACAATACCGGGCCAGCATGACGCAGGCCCGCGGTTTCAGGGAATGACCAACACTTTGCCCGGCATAGCATTACCAGCGATGTCACGCACCGCCTTGGCGAACCTTTCTAACCTGCCACGGTGAGCGATTAATgg                                    >  2:471320/1‑150 (MQ=255)
                                                                                                               aaTGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTATCCCGGCATAGCATTACGAGCGATGACACGAACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGc                                   >  2:1774222/1‑150 (MQ=255)
                                                                                                                aTGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCt                                  >  1:424501/1‑150 (MQ=255)
                                                                                                                 tGATCTGTTTTGCACAATACCGGGCCAGCATCCCGCTGGCCCGCGGTTTCAGGGAATGAGCAGCACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTc                                 >  2:1176598/1‑150 (MQ=255)
                                                                                                                 tGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTc                                 >  2:957401/1‑150 (MQ=255)
                                                                                                                  gATCTGTTTTGCACAACACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTcc                                >  1:914298/1‑150 (MQ=255)
                                                                                                                   aTCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCa                               >  2:127595/1‑150 (MQ=255)
                                                                                                                   aTCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCa                               >  1:158232/1‑150 (MQ=255)
                                                                                                                   aTCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGGTTGAGGGAATTGGCAACACGTTGACCGGAATAGGTTTTAGCGCGATGTAACGCACCGCCTGGGCGCAGGTTTCAAAGCTTGCCCGGTGAGGGGTTAAgggggccc                               >  2:1725080/1‑145 (MQ=255)
                                                                                                                    tCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAg                              <  2:950300/150‑1 (MQ=255)
                                                                                                                       gTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCt                           >  1:529618/1‑150 (MQ=255)
                                                                                                                        ttttGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCtt                          <  1:803599/150‑1 (MQ=255)
                                                                                                                         tttGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCGTTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTa                         >  1:1449806/1‑150 (MQ=255)
                                                                                                                            gCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGTCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACt                      <  1:763856/150‑1 (MQ=255)
                                                                                                                            gCACAATACCGGGCCAGCATCACGCTGGCCCGCGGGTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTTCGAGCGATGTCCCGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATGATTGGCTCCAGGCTTAACt                      >  2:689865/1‑150 (MQ=255)
                                                                                                                             cacaATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACtt                     <  2:222138/150‑1 (MQ=255)
                                                                                                                               caATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACTTAc                   >  1:1316395/1‑150 (MQ=255)
                                                                                                                                aaTCCCGGGCCAGCATCCCGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACTTACg                  <  1:1413017/150‑1 (MQ=255)
                                                                                                                                 aTACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGCAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACTTACGt                 >  2:269356/1‑150 (MQ=255)
                                                                                                                                  ccgcGGGCCAGCGTGATGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGCTGTCCCGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGCTTAATGGCTCCAGGCTTAACTTACGtt                >  1:1128408/4‑150 (MQ=255)
                                                                                                                                    ccGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACTTACGTTCt              >  1:1717208/1‑150 (MQ=255)
                                                                                                                                     cGGGCCAGCGTGATGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGCGCGATTAATGGCTCCAGGCTTATCTTACGTTCTg             >  1:973577/1‑150 (MQ=255)
                                                                                                                                     cGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTGTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACTTACGTTCTg             <  2:976384/150‑1 (MQ=255)
                                                                                                                                        gCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACTTACGTTCTGTCa          <  1:894852/150‑1 (MQ=255)
                                                                                                                                        gCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGATTCCACGGTGAGCGATTAATGGCTCCAGGCTTACCTTACGTTCTGTCa          >  2:251440/1‑150 (MQ=255)
                                                                                                                                        ccccgcagcaCGCGGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCGCCAGGCTTAACTTACGTTCTGTCa          <  2:1171162/146‑1 (MQ=255)
                                                                                                                                         ccAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACTTACGTTCTGTCAg         <  2:650333/150‑1 (MQ=255)
                                                                                                                                         ccAGCATCACGCTGGCCCGCGGGTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACTTACGTTCTGTCAg         <  1:1692033/150‑1 (MQ=255)
                                                                                                                                          cAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTTAGCGATTAATGGCTCCAGGCTTAACTTACGTTCTGTCAGc        <  2:320232/150‑1 (MQ=255)
                                                                                                                                             cATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCACAGCATCCACGGTGAGCGATTAATGGCTCCAGGCTTAACTTACGGTCTGTCAGCAAc     >  1:1679695/1‑150 (MQ=255)
                                                                                                                                              aTCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACTTACGTTCTGTCAGCAAcc    <  1:1010318/150‑1 (MQ=255)
                                                                                                                                                cACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACTTACGTTCTGTCAGCAACCgg  >  2:1007932/1‑150 (MQ=255)
                                                                                                                                                cACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACTTACGTTCTGTCAGCAACCgg  >  2:1014503/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
GTTCCCTGTTCATTCACTAATTGGATGATCTTATTCCTTCGCTCGAATGAATTCATCGTCATTTACCCATATTCAATTGTGGCTAGTGTAAACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACTTACGTTCTGTCAGCAACCGG  >  NC_000913/2171672‑2171965

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: