Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,230,416 G→C 5.1% intergenic (‑31/‑208) dusC ← / → yohJ tRNA‑dihydrouridine synthase C/UPF0299 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,230,4160GC 5.1% 396.2 / 5.8 123intergenic (‑31/‑208)dusC/yohJtRNA‑dihydrouridine synthase C/UPF0299 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (68/48);  new base C (4/3);  total (72/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 127 total aligned reads displayed.

AGTTGATCCACCACGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCTGATTTCTTAATGTGATC  >  NC_000913/2230267‑2230565
                                                                                                                                                     |                                                                                                                                                     
agTTGATCCACCACGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATg                                                                                                                                                       <  2:1852974/150‑1 (MQ=255)
agTTGATCCACCACGCGGACAAACTCGGTGATGCACAGATCGTAGACGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCAAATCATCACCCGCAAAAAAATGAGGCGCTATg                                                                                                                                                       >  2:1048702/1‑150 (MQ=255)
      tCCACCACGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAgcg                                                                                                                                                 >  1:1698116/1‑150 (MQ=255)
        caccacGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGcc                                                                                                                                               >  2:753140/1‑150 (MQ=255)
        caccacGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGcc                                                                                                                                               >  2:1904027/1‑150 (MQ=255)
         accacGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCt                                                                                                                                              >  2:1363025/1‑150 (MQ=255)
           cacGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAATCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTAACATAGCGCCtca                                                                                                                                            <  2:531815/150‑3 (MQ=255)
            acGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATg                                                                                                                                           <  1:1638294/150‑1 (MQ=255)
             cgcgGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATgg                                                                                                                                          <  1:1318922/150‑1 (MQ=255)
              gcgGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGAAATTCACGCACCAGAGAGTCAAGCTCTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTAACATAGCGCCtcattt                                                                                                                                         <  2:1481019/150‑6 (MQ=255)
               cgGACAAACTCGGTGATGCCCAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTATCGCCTATGGTc                                                                                                                                        >  1:1168467/1‑150 (MQ=255)
                   cAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCg                                                                                                                                    <  2:891135/150‑1 (MQ=255)
                      aCTCGTTGATGAAAAGATCGTATTCGTTAACTTCGGTCAGCAATTCACGCACCAGATAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGAt                                                                                                                                 <  1:941837/150‑1 (MQ=255)
                       cTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGAtt                                                                                                                                >  1:954447/1‑150 (MQ=255)
                           gTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAg                                                                                                                            <  2:1889465/150‑1 (MQ=255)
                              aTGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTc                                                                                                                         <  2:1698116/150‑1 (MQ=255)
                               tGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCt                                                                                                                        >  1:1494264/1‑150 (MQ=255)
                               tGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCt                                                                                                                        >  1:1170912/1‑150 (MQ=255)
                               tGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCt                                                                                                                        >  1:1663776/1‑150 (MQ=255)
                               tGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCt                                                                                                                        >  1:1091883/1‑150 (MQ=255)
                                gCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTg                                                                                                                       >  2:2809/1‑150 (MQ=255)
                                 cacaGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGCGTCAAGCACTCCCTCCATCGGTGCCAGTAGCCCACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAACGGTCTGc                                                                                                                      >  1:1142228/1‑150 (MQ=255)
                                     gATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAag                                                                                                                  >  1:883245/1‑150 (MQ=255)
                                        cGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAgg                                                                                                               >  2:164907/1‑150 (MQ=255)
                                        cGTAGTCGTTAACTTCGGTCAGCAATTCAAGCACCAGAGAGTCAAGAACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAAAGAGGCGCTATCTTAGAGACAAAGGTCAGCGGAATAAAGGACTGCAAAGAgg                                                                                                               >  1:278982/1‑150 (MQ=255)
                                             tCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATtg                                                                                                          >  2:380161/1‑150 (MQ=255)
                                              cGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATtgc                                                                                                         <  1:1278080/150‑1 (MQ=255)
                                                    cTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCaaa                                                                                                   >  1:508361/1‑150 (MQ=255)
                                                    cTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCaaa                                                                                                   >  1:492515/1‑150 (MQ=255)
                                                     ttCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAAc                                                                                                  <  1:1291947/150‑1 (MQ=255)
                                                           cAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAAt                                                                                            >  2:1496128/1‑150 (MQ=255)
                                                           cAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAAt                                                                                            >  2:75155/1‑150 (MQ=255)
                                                           cAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAAt                                                                                            >  1:958652/1‑150 (MQ=255)
                                                            aGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATc                                                                                           >  2:1093663/1‑150 (MQ=255)
                                                             gCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACCCGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGAGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATcc                                                                                          >  1:164606/1‑150 (MQ=255)
                                                             gCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATcc                                                                                          >  1:1911250/1‑150 (MQ=255)
                                                             gCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATcc                                                                                          >  1:1731044/1‑150 (MQ=255)
                                                             gCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATcc                                                                                          >  1:854008/1‑150 (MQ=255)
                                                                aTTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCctc                                                                                       >  1:781985/1‑150 (MQ=255)
                                                                aTTCAAGCACCAGATATTCAAGCACTCCCTCCATCGGTGCCATTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCATCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCTATCCctc                                                                                       <  2:1672369/150‑1 (MQ=255)
                                                                      gCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATg                                                                                 >  1:412866/1‑150 (MQ=255)
                                                                      gCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATg                                                                                 >  1:1401311/1‑150 (MQ=255)
                                                                       caccacAGAGTCAAGCACTCCCTCCATCGGGGCTATTAAAACACTCATATCATCACCAGCAATATAATGACGCGCTATCTTAGCGCCTATGGTCTGCGGATTAAAGTTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGc                                                                                <  2:1249129/150‑1 (MQ=255)
                                                                       cACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTTTGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTAtcc                                                                                >  2:396123/1‑148 (MQ=255)
                                                                           agagagTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGa                                                                            >  2:1604119/1‑150 (MQ=255)
                                                                            gagagTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGAt                                                                           <  1:1634741/150‑1 (MQ=255)
                                                                            gagagTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGAt                                                                           <  1:1553381/150‑1 (MQ=255)
                                                                               agTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTa                                                                        <  1:435916/150‑1 (MQ=255)
                                                                                gTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTAt                                                                       >  2:932925/1‑150 (MQ=255)
                                                                                   aaGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGcc                                                                    >  2:691065/1‑150 (MQ=255)
                                                                                   aaGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCGATGTTAGCGCCTATGGGCAGCGGATTCAAGGGCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGcc                                                                    <  1:295769/150‑1 (MQ=255)
                                                                                    aGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTg                                                                  >  1:1498123/1‑150 (MQ=255)
                                                                                      cACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGa                                                                 <  2:213958/150‑1 (MQ=255)
                                                                                        ctccctccATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGt                                                               >  1:471051/1‑150 (MQ=255)
                                                                                           cctccATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGACTATGGTCAGCGGATTAAAGGTCTGCAAAGATGGATTGCTGCAAACTGCAATCCCTCACTATGCTTGATGTATGCCAGAGTTCg                                                            >  1:1038216/1‑150 (MQ=255)
                                                                                            ctccAGCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGc                                                           <  1:152502/150‑1 (MQ=255)
                                                                                              ccATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTg                                                         <  2:85895/150‑1 (MQ=255)
                                                                                              ccATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGAATGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTg                                                         >  2:1304512/1‑150 (MQ=255)
                                                                                              ccATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGACTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTg                                                         <  2:1490838/150‑1 (MQ=255)
                                                                                              ccATCGGTGACAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAAACCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTg                                                         >  2:1208807/1‑150 (MQ=255)
                                                                                                   ggTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAACGAGGGATTGCTGCAACCTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCAt                                                    >  1:118174/1‑150 (MQ=255)
                                                                                                   gctGCAAGTAACACACGCATTTCATCACCCTGAAAATTTTGAGGCGCTTTGTTAGCGCATATGGTCAGCGGATTAAAGGTCTGCAAAGTGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTTAGTTCAGTGCGCAt                                                    <  2:235598/148‑1 (MQ=255)
                                                                                                       ccAGTAACACACTCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGTATTAAAGTTCTTCAAAGAGGGATTGCTGCAAACTTCAATCCATCTCTATGCTTTATGTATGCCTGAGTTCGCTGCGCATTGCg                                                <  2:902382/150‑1 (MQ=255)
                                                                                                       ccAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCg                                                >  1:143601/1‑150 (MQ=255)
                                                                                                         aGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTa                                              >  2:469694/1‑150 (MQ=255)
                                                                                                           tAACACACGCATATCATCACCCGCAAAAAATTGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAAt                                            <  1:758800/150‑1 (MQ=255)
                                                                                                               gcacGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGCATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATACCTTAGTTCGCTGCGCAATGCGTAATGCCg                                        >  1:1243067/2‑150 (MQ=255)
                                                                                                               acacGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGATTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCg                                        >  2:1446159/1‑150 (MQ=255)
                                                                                                                  cGCATATCATCACCCGCAAAAAAATGAGGGGCTATGTTTGCGCCTATGGTCAGCGGTTTTAAGGTGTGCAAAGAGGGATTGCTGCAAACTGGAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCgggg                                     <  1:170135/150‑1 (MQ=255)
                                                                                                                      tatCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCg                                 >  2:1094167/1‑150 (MQ=255)
                                                                                                                      tatCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCg                                 >  2:1355563/1‑150 (MQ=255)
                                                                                                                      tatCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCg                                 >  1:347546/1‑150 (MQ=255)
                                                                                                                      tatCATCACCCGCAAAAAAATGAGGCGCTAGGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCg                                 >  2:961575/1‑150 (MQ=255)
                                                                                                                          atccCCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGaaaa                             <  1:681513/150‑1 (MQ=255)
                                                                                                                          atcaCCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGaaaa                             >  1:1881827/1‑150 (MQ=255)
                                                                                                                          atcaCCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGaaaa                             <  2:1911250/150‑1 (MQ=255)
                                                                                                                            cccccGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGaaaaaa                           <  2:1249275/148‑1 (MQ=255)
                                                                                                                             aCCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAAt                          >  1:709739/1‑150 (MQ=255)
                                                                                                                                cGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAg                       >  2:885936/1‑150 (MQ=255)
                                                                                                                                cGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAg                       >  1:1462093/1‑150 (MQ=255)
                                                                                                                                cGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAg                       >  1:878759/1‑150 (MQ=255)
                                                                                                                                cGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGGCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAg                       <  2:954447/150‑1 (MQ=255)
                                                                                                                                 gCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAgc                      >  1:946578/1‑150 (MQ=255)
                                                                                                                                  cAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAgcg                     <  2:1091883/150‑1 (MQ=255)
                                                                                                                                      gaccataGGCGCTAACATAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCTGa                 >  1:1584692/7‑150 (MQ=255)
                                                                                                                                        ccataGGCGCTAACATAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCATGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCTGAtt               >  2:1280992/5‑150 (MQ=255)
                                                                                                                                          ataGGCGCTAACATAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCTGATTTc             >  1:33134/3‑150 (MQ=255)
                                                                                                                                           gAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGGGTAATGCCGGGGAGCGAAAAAATAAGCGCTGATTTCt            <  1:875677/150‑1 (MQ=255)
                                                                                                                                           gAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCTGATTTCt            <  2:499371/150‑1 (MQ=255)
                                                                                                                                           gAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCTGATTTCt            <  1:164907/150‑1 (MQ=255)
                                                                                                                                            aGGCTCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCTGATTTCtt           <  1:1850918/150‑1 (MQ=255)
                                                                                                                                            aGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCTGATTTCtt           <  1:1095465/150‑1 (MQ=255)
                                                                                                                                            aGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCTGATTTCtt           <  2:6312/150‑1 (MQ=255)
                                                                                                                                              gcgcTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATTCCTGTCTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCTGATTTCTTaa         <  1:830705/150‑1 (MQ=255)
                                                                                                                                              gcgcTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCTGATTTCTTaa         <  1:1093663/150‑1 (MQ=255)
                                                                                                                                                gcTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCTGATTTCTTAAtg       <  1:1874907/150‑1 (MQ=255)
                                                                                                                                                gcTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCTGATTTCTTAAtg       <  1:575482/150‑1 (MQ=255)
                                                                                                                                                gcTATGGTAGCGCCTATGGTCAGCGGATTCCAGGTCTGCAAAGAGGGAGTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCTGATTTCTTAAtg       <  1:159217/150‑1 (MQ=255)
                                                                                                                                                   aTGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCTGATTTCTTAATGTGa    >  2:1689226/1‑150 (MQ=255)
                                                                                                                                                     gTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCTGATTTCTTAATGTGATc  <  2:1854901/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AGTTGATCCACCACGCGGACAAACTCGGTGATGCACAGATCGTAGTCGTTAACTTCGGTCAGCAATTCACGCACCAGAGAGTCAAGCACTCCCTCCATCGGTGCCAGTAACACACGCATATCATCACCCGCAAAAAAATGAGGCGCTATGTTAGCGCCTATGGTCAGCGGATTAAAGGTCTGCAAAGAGGGATTGCTGCAAACTGCAATCCCTCTCTATGCTTGATGTATGCCTGAGTTCGCTGCGCATTGCGTAATGCCGGGGAGCGAAAAAATAAGCGCTGATTTCTTAATGTGATC  >  NC_000913/2230267‑2230565

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: