Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 184,240 G→T 10.2% intergenic (‑145/+17) yaeH ← / ← yaeI UPF0325 family protein/phosphodiesterase with model substrate bis‑pNPP

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913184,2400GT10.2% 224.6 / 5.4 83intergenic (‑145/+17)yaeH/yaeIUPF0325 family protein/phosphodiesterase with model substrate bis‑pNPP
Reads supporting (aligned to +/- strand):  ref base G (41/32);  new base T (2/8);  total (43/40)
Fisher's exact test for biased strand distribution p-value = 4.38e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TACATAGCGTTACCTCAAAATGAGTCAGTAAATTGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCTTCG  >  NC_000913/184091‑184389
                                                                                                                                                     |                                                                                                                                                     
tACATAGCGTTACCTCAAAATGAGTCAGTAAATTGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCGCGACTGTGATCGCAGAAAGCCTTTTTCTTCCCGACTTCTATCATGGCGTAAAAATCCAAAAATTACCTGCTTTATTCTgg                                                                                                                                                       <  2:1122160/150‑1 (MQ=255)
 aCATTGCGTTACCTCAAAATGAGTCAGTAAATTGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTTTGATCTCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGt                                                                                                                                                      <  1:306610/150‑1 (MQ=255)
 aCATAGCGTTACCTCAAAATGAGTCAGTAAATTTTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGt                                                                                                                                                      <  2:538556/150‑1 (MQ=255)
 aCATAGCGGTACCTCAAACTGAGTCAGTAAATTGTGCTTATTTTAGCATTTGGCATGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGt                                                                                                                                                      <  2:304618/150‑1 (MQ=255)
    tAGCGTTACCTCAAAATGAGTCAGTAAATTGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGAt                                                                                                                                                   >  1:564240/1‑150 (MQ=255)
      gCGTTACCTCAAAATGAGTCAGTAAATTGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGTTGATaa                                                                                                                                                 >  2:824874/1‑150 (MQ=255)
       cGTTACCTCAAAATGAGTCAGTAAATTGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATaaa                                                                                                                                                <  2:46423/150‑1 (MQ=255)
       cGTTACCTCAAAATGAGTCAGTAAATTGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATaaa                                                                                                                                                <  1:1584922/150‑1 (MQ=255)
           aCCTCAAAATGAGTCAGTAAATTGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTc                                                                                                                                            <  1:1564656/150‑1 (MQ=255)
             cTCAAAATGAGTCAGTAAATTGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTCCCTGCTTTATTATGGTGATAAAATTCAc                                                                                                                                          >  1:937223/1‑150 (MQ=255)
             cTCAAAATGAGTCAGTAAATTGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCAc                                                                                                                                          >  2:1116530/1‑150 (MQ=255)
              tCAAAATGAGTCAGTAAATTGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACg                                                                                                                                         <  2:1755047/150‑1 (MQ=255)
                aaaaTGAGTCAGTAAATTGTGCTTATTTTATCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTTGTGATAAAATTCACGAt                                                                                                                                       <  1:1223834/150‑1 (MQ=255)
                aaaaTGAGTCAGTAAATTGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGAt                                                                                                                                       <  1:195868/150‑1 (MQ=255)
                 aaaTGAGTCAGTAAATTGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATc                                                                                                                                      <  2:1024340/150‑1 (MQ=255)
                 aaaTGAGTCAGTAAATTGTGCTTATTTTAGCATTTGGCATGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTATTCGCGACTTCTATCATGGCGTAAAAATCAAAAAAATACCTCCTTTATTCTCTTGATAAAATTAACGAta                                                                                                                                      >  1:1203310/1‑149 (MQ=255)
                   aTGAGTCAGTAAATTGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTa                                                                                                                                    <  1:199220/150‑1 (MQ=255)
                   aTGAGTCAGTAAATTGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTa                                                                                                                                    <  1:1297742/150‑1 (MQ=255)
                      aGTCAGTAAATTGTGCGTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATAACCTGCTTTATTCTGGTGAGAAAATTCACGATCTacac                                                                                                                                 <  1:1053688/150‑1 (MQ=255)
                       gTCAGTAAATTGTGCTTATTTTAGCATTTGGCCTGGCCCTCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTCGTGATAAAATTCACGATCTACAcc                                                                                                                                >  2:1336669/1‑150 (MQ=255)
                       gTCAGTAAATTGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACAcc                                                                                                                                <  1:1634879/150‑1 (MQ=255)
                       gTCAGTAAATTGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCGCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTATTTTTTTTATTGTTTTGTTTAAATTCTAGATCTACAcc                                                                                                                                <  2:1609034/150‑1 (MQ=255)
                         cAGTAAATTGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAg                                                                                                                              <  1:656074/150‑1 (MQ=255)
                          aGTAAATTGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACTTTATTTATTGTTTTGTTAAAATTCTCGATCTACACCAGt                                                                                                                             <  2:1051934/150‑1 (MQ=255)
                           gTAAATTGTGCTTATTTTAGCATTAGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCAACACCAGtt                                                                                                                            >  2:1018794/1‑150 (MQ=255)
                             aaaTTGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTcc                                                                                                                          >  2:1097197/1‑150 (MQ=255)
                               aTTGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAg                                                                                                                        >  1:453619/1‑150 (MQ=255)
                                    gCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCTAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCAAGCATCg                                                                                                                   >  1:350971/1‑150 (MQ=255)
                                    gCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTTTTTTTTTTAATGTTTTGTTTAAATTCTAGATCTACACCAGTTCCAGCATCg                                                                                                                   <  2:733218/150‑1 (MQ=255)
                                    gCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCg                                                                                                                   >  1:1806824/1‑150 (MQ=255)
                                       tATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTa                                                                                                                <  1:597746/150‑1 (MQ=255)
                                                 tttGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTTTTTTTTTTATTGTTTTGTTTAAATTCTAGATCTACACCAGTTCCAGCATCGTTACTTCCGggcg                                                                                                      <  2:1785568/150‑1 (MQ=255)
                                                 tttGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCgggag                                                                                                      >  1:264119/1‑148 (MQ=255)
                                                  ttGGCCTGGCCCGCCCGGCTCGACTGAGATCGCAGAAAGCCTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCGGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGggcggc                                                                                                    <  1:1287848/150‑1 (MQ=255)
                                                        tggccCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTc                                                                                               <  1:1766349/150‑1 (MQ=255)
                                                             cgcccgGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTTTTTGTTTTATTGTTTTGTTTAAATTCTAGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACg                                                                                          <  2:806788/150‑1 (MQ=255)
                                                             cgcccgGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCCCGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACg                                                                                          <  1:852699/150‑1 (MQ=255)
                                                                ccgGCTAGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAATAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCaa                                                                                       <  1:508742/150‑1 (MQ=255)
                                                                   gCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATGCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGGTACTTCCGGGCGGCAGTTCAGACGCAAAcc                                                                                    <  1:1114455/150‑1 (MQ=255)
                                                                    cTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCa                                                                                   <  1:1661593/150‑1 (MQ=255)
                                                                    cTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGATCCAGCATCGATACTTCCGGGCGGCAGTTCAGACGCAAACCa                                                                                   <  1:344304/150‑1 (MQ=255)
                                                                      cGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACTATCTACACCAGTTCCAGCATCTTTACTTCCGGGCGGCAGTTCAGACGCAAACCATa                                                                                 >  2:822549/1‑150 (MQ=255)
                                                                      cGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATa                                                                                 >  1:1791399/1‑150 (MQ=255)
                                                                       gACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCAGCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATAc                                                                                <  1:1017464/150‑1 (MQ=255)
                                                                          tgtgATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACaaa                                                                             >  1:471445/1‑150 (MQ=255)
                                                                            tgATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACATGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACt                                                                           >  2:472174/1‑150 (MQ=255)
                                                                              aTCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAACCTGc                                                                         >  1:276720/1‑150 (MQ=255)
                                                                              aTCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGc                                                                         >  1:541533/1‑150 (MQ=255)
                                                                                cGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGccc                                                                       >  1:494104/1‑150 (MQ=255)
                                                                                 gCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCcca                                                                      >  2:1154731/1‑150 (MQ=255)
                                                                                  cAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCccac                                                                     >  1:443276/1‑150 (MQ=255)
                                                                                        gCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAACCCATACAAACTGCccacgccacg                                                               >  1:721834/1‑150 (MQ=255)
                                                                                        gCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCccacgccacg                                                               <  1:572924/150‑1 (MQ=255)
                                                                                        gCCTTTTTCTTACAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCTTTACTTACGGTCGGCAGTTCATACGCAAACCATACAAACTGCccacgccaag                                                               >  1:1162015/1‑148 (MQ=255)
                                                                                         ccTTTTTCTTCCAGACTTCTATGATGGCGTAAAAATCAAAAAATTACCTGATTTATTCTGGTGATAAAATTCACGATCTACAACAGTTCCAGCATCGTTACTTCCTGGCGTCAGTTCAGCAGCAAACCATACAAAATTCccacgacactg                                                              >  1:324986/1‑148 (MQ=255)
                                                                                         ccTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACgg                                                              >  2:437175/1‑150 (MQ=255)
                                                                                          cTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACggg                                                             >  2:999550/1‑150 (MQ=255)
                                                                                           tttttCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGt                                                            <  1:466705/150‑1 (MQ=255)
                                                                                             tttCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTtg                                                          <  1:789106/150‑1 (MQ=255)
                                                                                              ttcttcCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTtgt                                                         >  1:786054/1‑150 (MQ=255)
                                                                                                cttcCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGCCGCAAACCATACAAACTGCCCACGCCACGGGTTgtgt                                                       >  1:1532151/1‑150 (MQ=255)
                                                                                                cttcCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAAATCACGATCTACAACAGTTCCAGCATCGTTACTTCCGGTCTTCAGTTAAGACTCAAACAATAAAAACTGCAAACGACACGGGAtgtat                                                       >  1:227311/1‑148 (MQ=255)
                                                                                                    cAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGAt                                                   >  2:1004331/1‑150 (MQ=255)
                                                                                                    cAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCAAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAAATGCCCACGCCACGGGTTGTGTAGAt                                                   >  1:1207313/1‑150 (MQ=255)
                                                                                                     ataCTTCTATCATGGCGTAAAAATCAAATAATTTCCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGAtg                                                  <  1:1844670/148‑1 (MQ=255)
                                                                                                         ttCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTc                                              >  1:1860588/1‑150 (MQ=255)
                                                                                                           cTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCCCGGGTTGTGTACATGTGTCtt                                            >  2:1195749/1‑150 (MQ=255)
                                                                                                               aaTGGAGTAAAAATCAATATATTTTTTTTTTTATTGTTTTGTTTAAATTCTAGATCGACACCAGTTTCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCAGACAAACTGCCCACGCCACGGGTTGTGTAGATGTGGCtggcgc                                        <  2:1813274/149‑6 (MQ=255)
                                                                                                                aTGGCGTTAAAATCAAAAAATTACCTGCTTTATTCTGGGGCTAAAATTCACGATCTACACCAGTTCGAGGATCGTTACTTGCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCAAGCCACGGGTTGTGTAGATGTGTCTTTCGcc                                       <  1:16404/150‑1 (MQ=255)
                                                                                                                aTGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGcc                                       >  1:1621304/1‑150 (MQ=255)
                                                                                                                    cGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTTTCTTTCGCCAAAg                                   >  2:533091/1‑150 (MQ=255)
                                                                                                                      tAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATCCAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGc                                 <  1:657511/150‑1 (MQ=255)
                                                                                                                       aaaaaTCAAAAAATTTTTTTTTTTATTTTGTTGATTAAATTCTAGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCa                                <  2:264119/150‑1 (MQ=255)
                                                                                                                       aaaaaTCAAAAAATTACCTGCGTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTGCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCa                                <  1:1105068/150‑1 (MQ=255)
                                                                                                                         aaaTCAAAAAATTTTTTTTTTTATTGTTTTGTTTAAATTCTAGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGAAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCAtt                              <  2:1288646/150‑1 (MQ=255)
                                                                                                                         aaaTCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCAtt                              >  2:318633/1‑150 (MQ=255)
                                                                                                                            tCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTaa                           >  1:1661810/1‑150 (MQ=255)
                                                                                                                              aaaaaaTTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATc                         >  1:1891275/1‑150 (MQ=255)
                                                                                                                                            tttATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGt           >  2:1615116/1‑150 (MQ=255)
                                                                                                                                             ttATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGtt          >  2:1427512/1‑150 (MQ=255)
                                                                                                                                               aTTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGTTTGTGTAGATGTGTCTATCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTa        >  1:738583/1‑150 (MQ=255)
                                                                                                                                                 tCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTCACGTTTAtg      >  1:473953/1‑149 (MQ=255)
                                                                                                                                                  cTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCAACGTGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCt     <  1:1745089/150‑1 (MQ=255)
                                                                                                                                                   tGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCACAGGCATTTAATCCGGCGACGTAACGTTTATCtt    >  1:1412276/1‑150 (MQ=255)
                                                                                                                                                     gTGATAAAATTCACGATCTACACCAGTTCCCGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAACCCATCCAAACTGCCCACGCCCCGTGTGCTGTAGATGTGTCTTTCGCAAAAGGCATGTACTCCGGCGACGTAACGTTTATCTTCg  >  1:1632648/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TACATAGCGTTACCTCAAAATGAGTCAGTAAATTGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCTTCG  >  NC_000913/184091‑184389

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: