Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,286,139 A→T 5.7% intergenic (+3/‑72) yejM → / → proL essential inner membrane DUF3413 domain‑containing protein; lipid A production and membrane permeability factor/tRNA‑Pro

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,286,1390AT 5.7% 339.8 / 7.5 125intergenic (+3/‑72)yejM/proLessential inner membrane DUF3413 domain‑containing protein; lipid A production and membrane permeability factor/tRNA‑Pro
Reads supporting (aligned to +/- strand):  ref base A (60/57);  new base T (3/4);  total (64/61)
Fisher's exact test for biased strand distribution p-value = 7.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.37e-01

Only 100 of 129 total aligned reads displayed.

ACCACCCCGAAAAAGACGCTGGTACTGAACAATAACGGTAAATACCGCACTTACAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCA  >  NC_000913/2285990‑2286287
                                                                                                                                                     |                                                                                                                                                    
        gAAAAAGACGCTGGTACTGACCAATAACGGTAAATACCGCACTTACAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATaaa                                                                                                                                              >  1:1015823/1‑150 (MQ=255)
         aaaaaGACGCTGGTACTGAACAATAACGGTAAATACCGCACTTACAACTTACGTGTTGATAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAAt                                                                                                                                             <  1:210638/150‑1 (MQ=255)
         aaaaaGACGCTGGTACTGAACAATAACGGTAAATACCGCACTTACAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAAt                                                                                                                                             >  1:481130/1‑150 (MQ=255)
            aaGACGCTGGTACTGAACAATAACGGTAAATACCGCACTTACAACTTACGGGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAg                                                                                                                                          <  2:765096/150‑1 (MQ=255)
             aGACGCTGGTACTGAACAATAACGGTAAATACCGCACTTACAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGt                                                                                                                                         >  1:1031799/1‑150 (MQ=255)
              tactcTGGTACTGAACAATAACCGTAAATACCGCACTTACAACTTTCGTGGTGAAAGAGTGAAAGTTGTAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTTATAATTAATCAGtt                                                                                                                                        <  1:139156/146‑1 (MQ=255)
                  cTGGTACTGAACAATAACGGTAAATACCGCACTTACAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTTATAATTAATCAGTTAGCg                                                                                                                                    <  2:882692/150‑1 (MQ=255)
                  cTGGTACTGAACAATAACGGTAAATACCGCACTTAAAACTTACGTGGTGAAAGAGTTAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTGTATCGCTAACTGATTTATAATTAATCAGTTAGCg                                                                                                                                    <  1:1400339/150‑1 (MQ=255)
                   tGGTACTGAACAATAACGGTAAATACCGCACTTACAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGa                                                                                                                                   <  2:807386/150‑1 (MQ=255)
                    ggTACTGAACAATAACGGTAAATACCGCACTTACAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGaa                                                                                                                                  >  1:1730961/1‑150 (MQ=255)
                     gTACTGAACAATAACGGTAAATACCGCACTTACAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGaaa                                                                                                                                 >  1:1299443/1‑150 (MQ=255)
                       aCTGAACAATAACGGTAAATACCGCACTTACCACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAata                                                                                                                               <  1:850141/150‑1 (MQ=255)
                         tGAACAATAACGGTAAATACCGCACTTACAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATc                                                                                                                             >  2:1025242/1‑150 (MQ=255)
                           aaCAATAACGGTAAATACCTCAATTACAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCtt                                                                                                                           <  2:892794/150‑1 (MQ=255)
                             cAATAACGGTAAATACCGCACTTACAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTaa                                                                                                                         >  1:741815/1‑149 (MQ=255)
                             cAATAACGGTAAATACCGCACTTACAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTaa                                                                                                                         >  2:534775/1‑149 (MQ=255)
                              aaTAACGGTAAATACCGAACTTACAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACt                                                                                                                        <  2:1842919/150‑1 (MQ=255)
                                 aaCGGTAAATACCGCACTTACAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGc                                                                                                                     >  2:1716166/1‑150 (MQ=255)
                                 aaCGGTAAATACCGCACTTACAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGc                                                                                                                     >  2:1684133/1‑150 (MQ=255)
                                 aaCGGTAAATACCGCACTTACAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGc                                                                                                                     >  2:1042677/1‑150 (MQ=255)
                                    ggTAAATACCGCACTTACAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCaaa                                                                                                                  >  1:1580406/1‑149 (MQ=255)
                                       aaaTACCGCACTTACAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCgg                                                                                                               >  2:788267/1‑150 (MQ=255)
                                         aTACCGCACTTACAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGgtg                                                                                                             <  1:1757936/150‑1 (MQ=255)
                                           aCCGCACTTACAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGtgtg                                                                                                           <  1:1836043/150‑1 (MQ=255)
                                           aCCGCACTTACAACTTACGAGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGATAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGtgtg                                                                                                           <  2:470830/150‑1 (MQ=255)
                                           aCCGCACTTACAACTAACGTTGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCGGACAGACGAGAATCGTTTTCTCGCTAACTGATTAATTATAAATCAGATAGCGAAATATCTTACTTGCAATCGGtgtg                                                                                                           <  2:1405442/150‑1 (MQ=255)
                                             cGCACTTACAACTTACGTGGTGAAAGAGTGAAAGATGATAAACCACAGTTAAGTTTGTTATTGCAAGAGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTATTTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGa                                                                                                         >  2:939291/1‑150 (MQ=255)
                                             cGCACTTACAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGa                                                                                                         >  1:1333951/1‑150 (MQ=255)
                                                     cAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGgta                                                                                                 >  1:1602137/1‑150 (MQ=255)
                                                      aaCTTACGTGGTGAAAGAGTGAAATATGAAAAACCACAGTTAAGTTTTTTTTTGCAAGTGCTGACAGACGAGAAGCGGTTTATCGCTAACTGATTAATTATAAATCAGTAAGCGAAAAATCTTACTTGCAATCGGTGTGGAAAACGgtag                                                                                                <  2:307201/150‑1 (MQ=255)
                                                      aaCTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGgtag                                                                                                <  1:1780267/150‑1 (MQ=255)
                                                      aaCTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGgtag                                                                                                <  1:785080/150‑1 (MQ=255)
                                                      aaCTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGgtag                                                                                                <  2:1834249/150‑1 (MQ=255)
                                                      aaCTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGgtag                                                                                                <  1:491589/150‑1 (MQ=255)
                                                         ttACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTAt                                                                                             <  2:618531/150‑1 (MQ=255)
                                                          tACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTAtt                                                                                            >  2:1431808/1‑150 (MQ=255)
                                                               ggtgAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTagcag                                                                                       >  1:1429684/1‑150 (MQ=255)
                                                                 tgaatgagtgaaagaTGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGGGGAAAACGGTAGTATTAGCAGcc                                                                                     <  2:1799038/150‑1 (MQ=255)
                                                                 tgaaagagtgaaagaTGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGcc                                                                                     >  2:1583790/1‑150 (MQ=255)
                                                                  gaaagagtgaaagaTGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTTATAATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCa                                                                                    >  1:1550658/1‑150 (MQ=255)
                                                                  gaaagagtgaaagaTGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCa                                                                                    >  1:720881/1‑150 (MQ=255)
                                                                  gaaagagtgaaagaTGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCa                                                                                    <  2:1555552/150‑1 (MQ=255)
                                                                  gaaagagtgaaagaTGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGAAGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTTGAAAACGGTAGTATTAGCAGCCa                                                                                    >  1:962037/1‑150 (MQ=255)
                                                                    aagagtgaaagaTGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACg                                                                                  <  1:1697314/150‑1 (MQ=255)
                                                                    aagagtgaaagaTGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACg                                                                                  <  2:750616/150‑1 (MQ=255)
                                                                    aagagtgaaagaTGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACg                                                                                  >  2:1135655/1‑150 (MQ=255)
                                                                     agagtgaaagaTGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGa                                                                                 >  2:545914/1‑150 (MQ=255)
                                                                      gagtgaaagaTGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAg                                                                                <  1:1512798/150‑1 (MQ=255)
                                                                        gtgaaagaTGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGCCGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTc                                                                              >  2:291114/1‑150 (MQ=255)
                                                                        gtgaaagaTGAAAAACCACAGTTAAGTTTGTCATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTc                                                                              >  2:1173515/1‑150 (MQ=255)
                                                                         ggaaagaTGAAAAACCTCAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGATATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCg                                                                             <  1:443032/149‑1 (MQ=255)
                                                                          gaaagaTGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGCCAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCgg                                                                            >  2:1649016/1‑150 (MQ=255)
                                                                            aagaTGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCa                                                                          >  2:104382/1‑150 (MQ=255)
                                                                              gctcaaaaaCCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTATATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACg                                                                        <  1:458954/146‑1 (MQ=255)
                                                                                tGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTa                                                                      >  1:18865/1‑150 (MQ=255)
                                                                                  aaaaaCCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGCCGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCTGTGTGGAAAAAGGTAGTATTAGCAGACACGATTCTGCACGTAgc                                                                    >  1:1712398/1‑150 (MQ=255)
                                                                                  aaaaaCCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAgc                                                                    <  2:185735/150‑1 (MQ=255)
                                                                                    aaaCCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAgcgc                                                                  <  1:1173824/150‑1 (MQ=255)
                                                                                      aCCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAg                                                                >  1:800336/1‑150 (MQ=255)
                                                                                       ccACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGc                                                               >  2:191800/1‑150 (MQ=255)
                                                                                        cacaGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGcc                                                              >  2:1332198/1‑150 (MQ=255)
                                                                                           aGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATCATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGTCACGTAGCGCAGCCTgg                                                           <  1:553121/150‑1 (MQ=255)
                                                                                             ttAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTa                                                         <  2:557033/150‑1 (MQ=255)
                                                                                             ttAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCATACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTa                                                         <  2:597724/150‑1 (MQ=255)
                                                                                              tAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAg                                                        <  1:665463/150‑1 (MQ=255)
                                                                                                   ttGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCAc                                                   <  1:104382/150‑1 (MQ=255)
                                                                                                     gTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCGGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCg                                                 >  1:606286/1‑150 (MQ=255)
                                                                                                        aTTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAATTATCTTACTTGCAATCGGTGTGGAAACCGGTAGTATTTGCAGCCACGAGTCGGCACGTAGAGCAGCCTGCTAGCGCACCGTCa                                              >  1:164747/1‑150 (MQ=255)
                                                                                                        aTTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCa                                              <  1:1894632/150‑1 (MQ=255)
                                                                                                        aTTGCAAGTGCTGACAGACGAGAAGCGTTTAATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGTTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCa                                              >  1:941437/1‑150 (MQ=255)
                                                                                                         ttGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCCGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCAt                                             >  2:1696933/1‑150 (MQ=255)
                                                                                                           gCAGGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATgg                                           <  2:936650/150‑1 (MQ=255)
                                                                                                            cAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGAAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATggg                                          >  1:1897507/1‑150 (MQ=255)
                                                                                                             aaGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATgggg                                         <  2:608597/150‑1 (MQ=255)
                                                                                                                   tGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCgg                                   >  1:978531/1‑150 (MQ=255)
                                                                                                                    gACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCggg                                  <  2:481130/150‑1 (MQ=255)
                                                                                                                    gACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCggg                                  <  2:825384/150‑1 (MQ=255)
                                                                                                                    gACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAACGCAGCCTGGTAGCGCACCGTCATGGGGTGTCggg                                  <  1:1014841/150‑1 (MQ=255)
                                                                                                                     aCAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGGGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCgggg                                 <  1:1716166/150‑1 (MQ=255)
                                                                                                                      cAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCggggg                                <  2:1730961/150‑1 (MQ=255)
                                                                                                                        gACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGta                              >  2:913033/1‑149 (MQ=255)
                                                                                                                           gagaAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGTCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGa                           >  2:1456946/1‑150 (MQ=255)
                                                                                                                            agaAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGTACGTAGCGCAGCCTGGTAGCGAACCGTCATGGGGTCTCGGGGGTCGGAg                          >  2:232964/1‑150 (MQ=255)
                                                                                                                             gacgCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAgg                         <  1:1196543/147‑1 (MQ=255)
                                                                                                                                  gTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCaa                    <  1:1794857/150‑1 (MQ=255)
                                                                                                                                  gTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGAAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCaa                    >  1:220234/1‑150 (MQ=255)
                                                                                                                                   ttttATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCaaa                   <  2:460376/150‑1 (MQ=255)
                                                                                                                                      tATCGCTAACTGATTGATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATcc                >  2:1560457/1‑150 (MQ=255)
                                                                                                                                      tATCGCTAACTGATAAATTATAAATCAGTTAGCGAAATATCTTACTTGCCATCGGTGGGGAACACGGTACTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATcc                <  1:295439/150‑1 (MQ=255)
                                                                                                                                       aTCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCct               >  1:313271/1‑150 (MQ=255)
                                                                                                                                         cGCTAACTGATTTATAATTAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCctct             >  2:1003083/1‑150 (MQ=255)
                                                                                                                                         cGCTAACTGATAAATTATATATCAGTTAGCGAAATATCTTACTTGCAATCGGGGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCTCGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCctct             <  1:1157222/150‑1 (MQ=255)
                                                                                                                                          gCTAACTGATTTATAATTAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGACATGGGGTGTCGGGGGTCGGAGGTTCAAATCCtctc            >  1:703571/1‑150 (MQ=255)
                                                                                                                                             aaCTGATTAATTATAAATCAGTTAGCGAAATATCTTTCTTGCAATCGGTGCGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTg         <  1:1220881/150‑1 (MQ=255)
                                                                                                                                               cTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGcc       >  1:1245920/1‑150 (MQ=255)
                                                                                                                                               cTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGcc       >  2:588227/1‑150 (MQ=255)
                                                                                                                                                tGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCg      >  1:1905365/1‑150 (MQ=255)
                                                                                                                                                 gATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGAAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGa     >  2:1099259/1‑150 (MQ=255)
                                                                                                                                                  attaattaTAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGAc    >  2:1862457/1‑150 (MQ=255)
                                                                                                                                                   ttaattaTAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGTGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGccc   >  1:1375682/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
ACCACCCCGAAAAAGACGCTGGTACTGAACAATAACGGTAAATACCGCACTTACAACTTACGTGGTGAAAGAGTGAAAGATGAAAAACCACAGTTAAGTTTGTTATTGCAAGTGCTGACAGACGAGAAGCGTTTTATCGCTAACTGATTAATTATAAATCAGTTAGCGAAATATCTTACTTGCAATCGGTGTGGAAAACGGTAGTATTAGCAGCCACGAGTCGGCACGTAGCGCAGCCTGGTAGCGCACCGTCATGGGGTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCA  >  NC_000913/2285990‑2286287

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: