Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 240,840 T→G 5.3% intergenic (+24/+19) ivy → / ← fadE inhibitor of c‑type lysozyme, periplasmic/acyl coenzyme A dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913240,8400TG 5.3% 416.5 / 5.5 139intergenic (+24/+19)ivy/fadEinhibitor of c‑type lysozyme, periplasmic/acyl coenzyme A dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (69/61);  new base G (6/3);  total (75/64)
Fisher's exact test for biased strand distribution p-value = 5.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 142 total aligned reads displayed.

CGTCGCAAGAGAAACTCACCTGGCTGAATGTGAACGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCT  >  NC_000913/240692‑240988
                                                                                                                                                    |                                                                                                                                                    
cGTCGCAAGAGAAACTCACCTGGCTGAATGTGAACGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCtt                                                                                                                                                     >  1:1140792/1‑150 (MQ=255)
 gTCGCAAGAGAAACTCACCTGGCTGAATGTGAACGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCttt                                                                                                                                                    >  2:824637/1‑150 (MQ=255)
 gTCGCAAGAGAAACTCACCTGGCTGAATGTGAACGATGCGCTTTCGATTGATCGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTTGCGGATAAAGAAACGGAGCGttt                                                                                                                                                    >  2:824641/1‑150 (MQ=255)
   ctcAAGAGAAACTCACCTGGCTGAATGTGAACGATGCGCTTTCGATTGATGTTAAAACGGTGTTGTTCGCTGCGTTGACCGGCCGACTGGAAAACCATCCGGATGGCTTAAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCg                                                                                                                                                  <  1:354937/148‑1 (MQ=255)
   cGCAAGAGAAACTCACCTGGCTGAATGTGAACGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCg                                                                                                                                                  >  1:422595/1‑150 (MQ=255)
    gCAAGAGAAACTCACCTGGCTGAATGTGAACGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCgg                                                                                                                                                 >  2:1172387/1‑150 (MQ=255)
     cAAGAGAAACTCACCTGGCTGAATGTGAACGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGc                                                                                                                                                >  2:1703766/1‑150 (MQ=255)
     cAAGAGAAACTCACCTGGCTGAATGTGAACGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGc                                                                                                                                                <  2:1089105/150‑1 (MQ=255)
     cAAGAGAAACTCACCTGGCTGAATGTGAACGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGAGTTGACCGTCTGCATGGAAAACCATCCGGATGGCTTTAATTTTAAATATTTAGACGATAAAGAAACGGAGCCTTTCgcc                                                                                                                                                >  1:138057/1‑148 (MQ=255)
      aaGAGAAACTCACCTGGCTGAATGTGAACGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCt                                                                                                                                               >  2:1835792/1‑150 (MQ=255)
          gaAACTCACCTGGCTGAATGTGAAAGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGt                                                                                                                                           <  1:899438/150‑1 (MQ=255)
           aaaCTCACCTGGCTGAATGTGAACGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCTTCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCGAAAGGCTCCGtt                                                                                                                                          <  1:52108/150‑1 (MQ=255)
               tCACCTGGCTGAATGTGAACGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTc                                                                                                                                      <  1:1906902/150‑1 (MQ=255)
               tCACCTGGCTGAATGTGAACGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTGTCGGCTCCGTTAtta                                                                                                                                      >  1:1403457/1‑149 (MQ=255)
                  ccTGGCTGAATGTGAACGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCAtt                                                                                                                                   >  1:1888955/1‑150 (MQ=255)
                    tGGCTGAATGTGAACGATGCGCGTTCGATTGCTGGTAACACGGTGTTGGTCGCGGCGTTGACCGGCAGCCTGGTAATCCATCCGGATGGCTTTAATTTTAAATAATTAGGGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTa                                                                                                                                 <  1:1672647/150‑1 (MQ=255)
                       cTGAATGTGAACGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTAcgc                                                                                                                              >  1:1525594/1‑150 (MQ=255)
                           aTGTGAACGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCt                                                                                                                          <  1:1052522/150‑1 (MQ=255)
                           aTGTGAACGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCt                                                                                                                          >  2:27588/1‑150 (MQ=255)
                             gtgAACGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTc                                                                                                                        <  1:1848944/150‑1 (MQ=255)
                              tgAACGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGATGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCa                                                                                                                       >  1:278243/1‑150 (MQ=255)
                              agAACGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCTGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCa                                                                                                                       >  2:570367/2‑150 (MQ=255)
                                aaCGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCTGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAAc                                                                                                                     <  1:1293004/150‑1 (MQ=255)
                                aaCGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAAc                                                                                                                     <  2:1557282/150‑1 (MQ=255)
                                aaCGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAAc                                                                                                                     <  2:1468630/150‑1 (MQ=255)
                                 aCGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACt                                                                                                                    <  2:441846/150‑1 (MQ=255)
                                 aCGATGCGCTATCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACt                                                                                                                    <  1:1482090/150‑1 (MQ=255)
                                  cGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAAATTTAAATAATTACCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACtt                                                                                                                   >  2:1042896/1‑150 (MQ=255)
                                     tGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTcc                                                                                                                <  1:1198383/150‑1 (MQ=255)
                                      gcgcTTTCTATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCg                                                                                                               <  2:1650657/150‑1 (MQ=255)
                                          tttCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCCCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACt                                                                                                           <  1:1475354/150‑1 (MQ=255)
                                             cGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCAGCACTTtc                                                                                                        >  2:237131/1‑150 (MQ=255)
                                             cGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTGTCGGCTCCGTTATTCATTTTCGCGGCTTCAACTGTCCGCACTTtc                                                                                                        >  2:192197/1‑150 (MQ=255)
                                             cGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCGTTCGTCTCCGTTATTCATTTACGCGGCTTCACCTATCCGCACTTtc                                                                                                        >  1:287825/1‑150 (MQ=255)
                                                 tGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCg                                                                                                    >  2:1675059/1‑150 (MQ=255)
                                                  gaaggTAAAACGGTGTTGTTCGCGGCCTAGACCGGCAGCCTGCAAAACCATCCGGATGGCATTAATTTTAAATAAATAGCGGATAAAGAAACGCAGCCTTTCCGCTCCGTTATTCATATACGCCGCTTCAACTTTCCGCACTTTCTcccg                                                                                                   >  2:91400/4‑148 (MQ=255)
                                                   aTGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGc                                                                                                  >  2:892736/1‑150 (MQ=255)
                                                    tGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACtt                                                                                                          <  1:1383799/141‑1 (MQ=255)
                                                    tGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAAAGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACtt                                                                                                          >  2:1383799/1‑141 (MQ=255)
                                                       tAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGTTGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACt                                                                                              <  1:1425091/150‑1 (MQ=255)
                                                       tAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACt                                                                                              >  1:315905/1‑150 (MQ=255)
                                                            cGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTAcc                                                                                         >  2:1042195/1‑150 (MQ=255)
                                                              gtgtTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCgg                                                                                       <  1:1753575/150‑1 (MQ=255)
                                                                     tCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTtcgtc                                                                                >  1:1023256/1‑150 (MQ=255)
                                                                      cgcgGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTTCCGGCGtcgtcg                                                                               >  2:1330249/1‑150 (MQ=255)
                                                                      cgcgGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTtcgtcg                                                                               <  2:1881136/150‑1 (MQ=255)
                                                                        cggcggTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGcc                                                                             <  1:750581/150‑1 (MQ=255)
                                                                         ggcgTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCGTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCa                                                                            <  1:785081/150‑1 (MQ=255)
                                                                           cgTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGc                                                                          <  1:459375/150‑1 (MQ=255)
                                                                             ttGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGctc                                                                        <  1:1276533/150‑1 (MQ=255)
                                                                             ttGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGctc                                                                        <  2:1724652/150‑1 (MQ=255)
                                                                             gtGACCGGCAGGCTGGAAAACGATCCGGATGGCTTTAATTTTAAATATTTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTTCGGGGCTTCAACTTTCCGCACTTTCTGCGGCAACTTTAGCGGCTTCGTCGCCAGctc                                                                        <  2:1043856/149‑1 (MQ=255)
                                                                              tGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGctct                                                                       >  2:625458/1‑150 (MQ=255)
                                                                                 ccGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTcc                                                                    <  1:449178/150‑1 (MQ=255)
                                                                                 ccGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTcc                                                                    <  2:278243/150‑1 (MQ=255)
                                                                                   ggCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCgg                                                                  >  2:1858793/1‑150 (MQ=255)
                                                                                   ggCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCGTTCGGCTCCGTTATTCATTTAAGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCCCCAGCTCATCCgg                                                                  >  2:749366/1‑150 (MQ=255)
                                                                                    gCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTATCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGa                                                                 >  1:254177/1‑150 (MQ=255)
                                                                                     cAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGAt                                                                <  1:850296/150‑1 (MQ=255)
                                                                                     cAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGAt                                                                <  1:1193061/150‑1 (MQ=255)
                                                                                       gCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCa                                                              >  1:848396/1‑150 (MQ=255)
                                                                                       gCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCa                                                              >  2:1619033/1‑150 (MQ=255)
                                                                                         cTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGATCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCaaa                                                            >  2:1152031/1‑150 (MQ=255)
                                                                                          tGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTATCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACTCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTAGTCGCCAGCTCTTCCGGATCAAAg                                                           <  2:204586/150‑1 (MQ=255)
                                                                                           ggAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGt                                                          >  2:79713/1‑150 (MQ=255)
                                                                                                      cGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCGTTTCGGGTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCAGCAACGTTa                                               >  1:910824/1‑150 (MQ=255)
                                                                                                      cGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTa                                               >  2:1435625/1‑150 (MQ=255)
                                                                                                      cGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGATCCGTTATTCATTTCCGCGGCTTCAACTTTACGCCCTTTCTCCGGAAACTTTACCCGCTTCGTCGCCAGCTATTCCTGACCAAAGTCATAGACGTTa                                               >  2:140558/1‑150 (MQ=255)
                                                                                                        gATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAAt                                             <  1:1809406/150‑1 (MQ=255)
                                                                                                        gATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCGTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAAt                                             <  2:1214924/150‑1 (MQ=255)
                                                                                                           ggCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACt                                          >  1:461595/1‑150 (MQ=255)
                                                                                                             cTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTgc                                        >  1:1345558/1‑150 (MQ=255)
                                                                                                             cTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTgc                                        >  1:1261458/1‑150 (MQ=255)
                                                                                                             cTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCAGGATCAAAGTCATCAACGTTAATACTgc                                        >  2:301338/1‑150 (MQ=255)
                                                                                                              tttAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTACCGCGGCTACAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAAAACTgcg                                       >  2:1371932/1‑150 (MQ=255)
                                                                                                                  aTTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGa                                   <  1:1511645/150‑1 (MQ=255)
                                                                                                                    tttAAATAATTAGCGGATAAAGAAACGGCGCCTTTCGGCACCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGCACAAAGTCATCAACGTTAATACTGCGCTGAcc                                 >  1:1509317/1‑149 (MQ=255)
                                                                                                                    tttAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCAGGCAACTTTACCGGCTTCGTCGCCAGCTCTGCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACg                                 >  2:1314724/1‑150 (MQ=255)
                                                                                                                    tttAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACgg                                >  1:185660/1‑150 (MQ=255)
                                                                                                                     ttAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACgg                                >  2:1556413/1‑150 (MQ=255)
                                                                                                                     ttAAATAATTAGCGGATAAAGAAACGGAGCATTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCAGCACTATCTCCGTCAAATTTACCGGCTTCGTCGCCAGATCGACCGGAGGAAAATCATCAAAGTTAAAACTGCGCATACgg                                >  1:194951/1‑150 (MQ=255)
                                                                                                                      tAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGc                               <  2:1140792/150‑1 (MQ=255)
                                                                                                                        aataatTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGACTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCtt                             >  1:1613928/1‑150 (MQ=255)
                                                                                                                            atTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTttctt                         >  1:1369188/1‑150 (MQ=255)
                                                                                                                             ttAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTttcttc                        <  2:187500/150‑1 (MQ=255)
                                                                                                                              tAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCa                       <  1:733775/150‑1 (MQ=255)
                                                                                                                               aGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAg                      <  2:779600/150‑1 (MQ=255)
                                                                                                                                gCGGATAAAGAAACGGAGCCTTTCGGCTCCGTAATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGCTCAAAGTCATCAACGTTAATACTGCGCAGCCGGCTTTGTTCAGc                     >  2:332700/1‑150 (MQ=255)
                                                                                                                                  ggATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTGAATACTGCGCAGCCGGCTTTCTTCAGCtt                   >  1:1334486/1‑150 (MQ=255)
                                                                                                                                    aTAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTc                 <  1:1845092/150‑1 (MQ=255)
                                                                                                                                       aaGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATAGTGCGCAGACGGCTTTCTTCAGCTTTCAcc              <  2:315905/150‑1 (MQ=255)
                                                                                                                                       aaGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGAAGTCTTTCTTCCGCTTTCAcc              >  2:171689/1‑150 (MQ=255)
                                                                                                                                        aataaCGGAGCCGAAAGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCa             >  2:1100692/4‑150 (MQ=255)
                                                                                                                                         ataaCGGAGCCGAAAGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAg            >  1:489186/3‑150 (MQ=255)
                                                                                                                                          aaaCGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGa           <  1:824637/150‑1 (MQ=255)
                                                                                                                                          aaaCGCAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGa           <  1:824641/150‑1 (MQ=255)
                                                                                                                                           aaCGGAGCCGAAAGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGaa          >  2:1745787/1‑150 (MQ=255)
                                                                                                                                                aGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCg     <  2:431962/150‑1 (MQ=255)
                                                                                                                                                   cTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCt  <  2:119374/150‑1 (MQ=255)
                                                                                                                                                   cTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCt  >  2:1658212/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CGTCGCAAGAGAAACTCACCTGGCTGAATGTGAACGATGCGCTTTCGATTGATGGTAAAACGGTGTTGTTCGCGGCGTTGACCGGCAGCCTGGAAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCT  >  NC_000913/240692‑240988

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: