Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,411,408 A→T 5.3% intergenic (‑56/+31) yfbS ← / ← yfbT putative transporter/sugar phosphatas

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,411,4080AT 5.3% 328.3 / 5.8 113intergenic (‑56/+31)yfbS/yfbTputative transporter/sugar phosphatas
Reads supporting (aligned to +/- strand):  ref base A (45/62);  new base T (4/2);  total (49/64)
Fisher's exact test for biased strand distribution p-value = 4.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.87e-01

Only 100 of 118 total aligned reads displayed.

CAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAACCGCAATG  >  NC_000913/2411260‑2411556
                                                                                                                                                    |                                                                                                                                                    
caaaGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTGAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCcgac                                                                                                                                                     >  2:561953/1‑150 (MQ=255)
 aaaGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCcgacg                                                                                                                                                    <  2:556128/150‑1 (MQ=255)
      gACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACg                                                                                                                                               >  2:1579424/1‑150 (MQ=255)
          gCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGc                                                                                                                                           <  2:1125869/150‑1 (MQ=255)
           cATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGTCTCGTCGGGGCt                                                                                                                                          <  2:1063526/150‑1 (MQ=255)
             tCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCAAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTaa                                                                                                                                        >  1:318473/1‑150 (MQ=255)
              ccATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAAGCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGCCGGGGCTaaa                                                                                                                                       >  2:1692715/1‑150 (MQ=255)
               cATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAAt                                                                                                                                      <  2:1625229/150‑1 (MQ=255)
                 tACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCATTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGTCTCGTCGGGGCtttttta                                                                                                                                    <  2:164465/150‑7 (MQ=255)
                        tctGCCCGTCGCAAACAAGACGATGGCAACGTCCAGTAATTAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGTGATGAGTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGTCTAAATCATTATCAg                                                                                                                             <  2:1082460/150‑1 (MQ=255)
                         ctGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAgg                                                                                                                            >  2:1603403/1‑150 (MQ=255)
                         ctGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAgg                                                                                                                            >  1:531942/1‑150 (MQ=255)
                           gCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGgtg                                                                                                                          >  2:1199043/1‑150 (MQ=255)
                             ccGTCGCAAACAAGACGATGGCAACGGCCAGTATTGAAAGCACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGAGTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGtt                                                                                                                        >  2:1238194/1‑150 (MQ=255)
                               gTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTc                                                                                                                      <  1:548288/150‑1 (MQ=255)
                                  gCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACt                                                                                                                   >  1:772989/1‑150 (MQ=255)
                                   cAAACAATAAGATGGCATCGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTg                                                                                                                  <  2:1732040/150‑1 (MQ=255)
                                   cAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTg                                                                                                                  >  1:1665840/1‑150 (MQ=255)
                                   cAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTg                                                                                                                  <  2:1116547/150‑1 (MQ=255)
                                   cAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATAATGATCAGGTGTTTCACTg                                                                                                                  >  1:810625/1‑150 (MQ=255)
                                    aaaCAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACACCTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGa                                                                                                                 >  1:1319784/1‑150 (MQ=255)
                                      aCAAGACGATGGCAACGGCCAGTAATGAAAGAAACCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTTAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGaat                                                                                                               <  1:1843754/150‑1 (MQ=255)
                                          gACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAataata                                                                                                           <  1:1673231/150‑1 (MQ=255)
                                          gACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAataata                                                                                                           <  1:66819/150‑1 (MQ=255)
                                           aCGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAAtaataa                                                                                                          >  1:147245/1‑150 (MQ=255)
                                              aTGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTc                                                                                                                      >  2:1318476/1‑135 (MQ=255)
                                              aTGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTc                                                                                                                      <  1:1318476/135‑1 (MQ=255)
                                                 gCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGc                                                                                                    <  2:1259529/150‑1 (MQ=255)
                                                   aaCGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCa                                                                                                  <  2:561067/150‑1 (MQ=255)
                                                   aaCGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAACCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCa                                                                                                  <  1:1382936/150‑1 (MQ=255)
                                                        ccAGTAATGAAAGAACCCAAATCAATTCACCTTTCACAACTTATCCTTGTTAATTTAGGGGTATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTgg                                                                                             <  1:938764/150‑1 (MQ=255)
                                                        ccAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTgg                                                                                             <  1:167017/150‑1 (MQ=255)
                                                        ccAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTgg                                                                                             <  1:695432/150‑1 (MQ=255)
                                                         cAGTAATGAAAGAACCCAAATCAATTTACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGc                                                                                            >  2:180432/1‑150 (MQ=255)
                                                          aGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGGTGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCt                                                                                           >  1:458353/1‑150 (MQ=255)
                                                           gTAATGAAAGAACCCAAATCAATTCACAGTTCACAACTTATCCTTTTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTg                                                                                          <  1:1825892/150‑1 (MQ=255)
                                                            tAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGt                                                                                         <  2:253653/150‑1 (MQ=255)
                                                               tGAAAGAACCCAAATCAATTCGCCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGATGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTg                                                                                      >  1:875268/1‑150 (MQ=255)
                                                                taaaGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTgg                                                                                     <  2:1815082/149‑1 (MQ=255)
                                                                gAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTgg                                                                                     >  2:1647598/1‑150 (MQ=255)
                                                                  aaGAACCAAAATCAATTCCCCGTTCACAACTTATCCTTGTTAATTGAGTGGGATGACTTGAATCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTc                                                                                   <  1:337417/150‑1 (MQ=255)
                                                                     aaCCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAAATGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTcaca                                                                                >  1:946857/1‑150 (MQ=255)
                                                                      aCCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAg                                                                               <  1:787690/150‑1 (MQ=255)
                                                                      aCCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAg                                                                               <  2:772989/150‑1 (MQ=255)
                                                                      aCCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAg                                                                               <  2:1602518/150‑1 (MQ=255)
                                                                      aCCCAAATCAATTCACCGTTCACAACTAATCCTTGTTATTTGCGGGGGATGACTTGATTCTGCCATAACAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGGCACAg                                                                               <  1:1012668/150‑1 (MQ=255)
                                                                       cccAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGt                                                                              >  2:330368/1‑150 (MQ=255)
                                                                       cccAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGt                                                                              >  1:590008/1‑150 (MQ=255)
                                                                        ccAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTa                                                                             >  2:725137/1‑150 (MQ=255)
                                                                          aaaTCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAAt                                                                           >  1:1105626/1‑150 (MQ=255)
                                                                           aaTCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAGTCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAAtt                                                                          <  2:203998/150‑1 (MQ=255)
                                                                              cAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGc                                                                       <  1:1472614/150‑1 (MQ=255)
                                                                              cAATTCACCGTTCACAACTTATCATGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCt                                                                      <  2:654440/150‑1 (MQ=255)
                                                                               aaTTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCt                                                                      >  2:280540/1‑150 (MQ=255)
                                                                                aTTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTc                                                                     >  2:425887/1‑150 (MQ=255)
                                                                                aTTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTc                                                                     >  1:1600145/1‑150 (MQ=255)
                                                                                aTTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTc                                                                     <  1:1868278/150‑1 (MQ=255)
                                                                                 ttCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTcc                                                                    >  1:19485/1‑150 (MQ=255)
                                                                                   cACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGACATTTGGCTGTTTGGTCACAGTAATTTGCTCCAg                                                                  >  2:1903042/1‑150 (MQ=255)
                                                                                   cACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACATGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAg                                                                  <  2:520422/150‑1 (MQ=255)
                                                                                          cacaACTTATCCTTGTTAATTGCGGGGGATGACTTGATTCTGCCAAAAAAAAGCCCCGACGAGACGGGGCTAAATCTTGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTgg                                                           <  2:1877299/150‑1 (MQ=255)
                                                                                          cacaACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTgg                                                           >  2:93626/1‑150 (MQ=255)
                                                                                           acaACTTATCCTTGTTATTGGAGGGGTATTACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTgga                                                          <  2:147245/150‑1 (MQ=255)
                                                                                           acaACTTATCCTTGTTAATTGCGGTGGATGACTTGATTCTGCCATAAAAACGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACAGAAAAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTgga                                                          <  2:1848535/150‑1 (MQ=255)
                                                                                           acaACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTgga                                                          <  1:670555/150‑1 (MQ=255)
                                                                                            caACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTggag                                                         <  2:1873422/150‑1 (MQ=255)
                                                                                             aaCTTATCCTTGTTAATTGAGGGGGAGGACTTGATTCTGCCAAAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTggagg                                                        <  2:585122/150‑1 (MQ=255)
                                                                                              aCTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTggagga                                                       >  2:983858/1‑150 (MQ=255)
                                                                                              aCTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTggagga                                                       <  1:1529560/150‑1 (MQ=255)
                                                                                               attATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGAc                                                      <  1:1428100/149‑1 (MQ=255)
                                                                                                 tATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCa                                                    >  2:1455870/1‑150 (MQ=255)
                                                                                                 tATCCTTGATAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCa                                                    <  1:1839230/150‑1 (MQ=255)
                                                                                                    ccTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCTCAGTAATTTGCTCCAGACTGTGGAGGACCaact                                                 >  2:1364793/1‑148 (MQ=255)
                                                                                                    ccTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGCCTAAATCATGATCAGGTGTTTCACTCAATAATAACATCGCCATTTGGCTGATTGGTCACACTAATTTGCTCCAGACTGTGGAGGACCAAAt                                                 >  1:119263/1‑150 (MQ=255)
                                                                                                           aaTTGAGGGGGATGACTTGATTCTGCCATAAAAAACCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTc                                          <  2:417580/150‑1 (MQ=255)
                                                                                                               gctggggATTACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCTCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTc                                      <  1:941208/147‑1 (MQ=255)
                                                                                                               gAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTc                                      >  1:764086/1‑150 (MQ=255)
                                                                                                               gAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTc                                      >  2:456581/1‑150 (MQ=255)
                                                                                                                      ttGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGcgcg                               <  2:1483327/149‑1 (MQ=255)
                                                                                                                       tGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCgg                              <  2:1369704/150‑1 (MQ=255)
                                                                                                                       tGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCgg                              <  2:1135172/150‑1 (MQ=255)
                                                                                                                        gACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCggg                             <  1:963441/150‑1 (MQ=255)
                                                                                                                         aCTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCgggg                            <  1:1511151/150‑1 (MQ=255)
                                                                                                                          cTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCACATCGACCTACTTCAGGCGCGGGGt                           >  2:1483816/1‑150 (MQ=255)
                                                                                                                          cTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGt                           >  1:113205/1‑150 (MQ=255)
                                                                                                                            tGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGGAt                         >  2:144032/1‑150 (MQ=255)
                                                                                                                               ttCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTg                      <  1:1199043/150‑1 (MQ=255)
                                                                                                                               ttCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGAGCAGGTGTGTCACTGAATAATAACATCGCCATTTGGCTGTTTGTTGACTGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGTTATCTg                      <  1:288519/150‑1 (MQ=255)
                                                                                                                                 cTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGcc                    <  1:1691663/150‑1 (MQ=255)
                                                                                                                                   gCCATCAAAACGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCgg                  <  2:158293/150‑1 (MQ=255)
                                                                                                                                    ccATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGa                 >  1:712900/1‑150 (MQ=255)
                                                                                                                                       tgatttaGCCCCGTCTCGTCGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCg              >  2:1045494/7‑150 (MQ=255)
                                                                                                                                       tgatttaGCCCCGTCTCGTCGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCg              >  1:1358664/7‑150 (MQ=255)
                                                                                                                                        gatttaGCCCCGTCTCGTCGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGCCCTCATTCAGGCGCGGGGTATCTGCCGGAGCGt             >  2:788937/6‑150 (MQ=255)
                                                                                                                                          aaaaGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTa           <  1:1680578/150‑1 (MQ=255)
                                                                                                                                          aaaaGCCACGACGAGACGGGGCTAAATCATGCTCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTAGCTGCCGGAGCGTTa           <  1:322295/150‑1 (MQ=255)
                                                                                                                                            taGCCCCGTCTCGTCGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAAc         >  1:1270490/2‑150 (MQ=255)
                                                                                                                                                   gacgaGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAACCGCAatg  >  1:1373176/1‑150 (MQ=255)
                                                                                                                                                   gacgaGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGGATCTGCCGGAGCGTTAACCGCAatg  <  1:60274/150‑1 (MQ=255)
                                                                                                                                                   gacgaGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGGTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAACCGCAatg  <  1:1152953/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CAAAGCGACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAACCGCAATG  >  NC_000913/2411260‑2411556

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: