Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,411,413 A→T 5.1% intergenic (‑61/+26) yfbS ← / ← yfbT putative transporter/sugar phosphatas

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,411,4130AT 5.1% 347.1 / 6.1 118intergenic (‑61/+26)yfbS/yfbTputative transporter/sugar phosphatas
Reads supporting (aligned to +/- strand):  ref base A (46/65);  new base T (4/2);  total (51/67)
Fisher's exact test for biased strand distribution p-value = 4.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.65e-01

Only 100 of 120 total aligned reads displayed.

GACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAACCGCAATGACAT  >  NC_000913/2411266‑2411560
                                                                                                                                                   |                                                                                                                                                   
gACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACg                                                                                                                                                   >  2:1579424/1‑150 (MQ=255)
    gCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGc                                                                                                                                               <  2:1125869/150‑1 (MQ=255)
     cATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGTCTCGTCGGGGCt                                                                                                                                              <  2:1063526/150‑1 (MQ=255)
         cATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAAt                                                                                                                                          <  2:1625229/150‑1 (MQ=255)
           tACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCATTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGTCTCGTCGGGGCtttttta                                                                                                                                        <  2:164465/150‑7 (MQ=255)
               cACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTGCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGAt                                                                                                                                    >  1:232997/1‑150 (MQ=255)
                 ctctGCCCGTCGCAAACAAGACGATGGCACCGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCa                                                                                                                                  >  2:1152953/1‑150 (MQ=255)
                  tctGCCCGTCGCAAACAAGACGATGGCAACGTCCAGTAATTAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGTGATGAGTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGTCTAAATCATTATCAg                                                                                                                                 <  2:1082460/150‑1 (MQ=255)
                   ctGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAgg                                                                                                                                >  1:531942/1‑150 (MQ=255)
                   ctGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAgg                                                                                                                                >  2:1603403/1‑150 (MQ=255)
                     gCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGgtg                                                                                                                              >  2:1199043/1‑150 (MQ=255)
                       ccGTCGCAAACAAGACGATGGCAACGGCCAGTATTGAAAGCACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGAGTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGtt                                                                                                                            >  2:1238194/1‑150 (MQ=255)
                            gCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACt                                                                                                                       >  1:772989/1‑150 (MQ=255)
                             cAAACAATAAGATGGCATCGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTg                                                                                                                      <  2:1732040/150‑1 (MQ=255)
                             cAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTg                                                                                                                      <  2:1116547/150‑1 (MQ=255)
                             cAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTg                                                                                                                      >  1:1665840/1‑150 (MQ=255)
                             cAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATAATGATCAGGTGTTTCACTg                                                                                                                      >  1:810625/1‑150 (MQ=255)
                              aaaCAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACACCTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGa                                                                                                                     >  1:1319784/1‑150 (MQ=255)
                                aCAAGACGATGGCAACGGCCAGTAATGAAAGAAACCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTTAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGaat                                                                                                                   <  1:1843754/150‑1 (MQ=255)
                                aCAAGACCATGGCAACGGCCAGTAATGAAAGAAACCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGaat                                                                                                                   <  2:263491/150‑1 (MQ=255)
                                    gACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAataata                                                                                                               <  1:66819/150‑1 (MQ=255)
                                    gACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAataata                                                                                                               <  1:1673231/150‑1 (MQ=255)
                                     aCGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAAtaataa                                                                                                              >  1:147245/1‑150 (MQ=255)
                                        aTGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTc                                                                                                                          <  1:1318476/135‑1 (MQ=255)
                                        aTGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTc                                                                                                                          >  2:1318476/1‑135 (MQ=255)
                                          ggCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCg                                                                                                         <  1:13964/150‑1 (MQ=255)
                                           gCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGc                                                                                                        <  2:1259529/150‑1 (MQ=255)
                                             aaCGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCa                                                                                                      <  2:561067/150‑1 (MQ=255)
                                             aaCGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAACCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCa                                                                                                      <  1:1382936/150‑1 (MQ=255)
                                                  ccAGTAATGAAAGAACCCAAATCAATTCACCTTTCACAACTTATCCTTGTTAATTTAGGGGTATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTgg                                                                                                 <  1:938764/150‑1 (MQ=255)
                                                   cAGTAATGAAAGAACCCAAATCAATTTACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGc                                                                                                >  2:180432/1‑150 (MQ=255)
                                                    aGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGGTGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCt                                                                                               >  1:458353/1‑150 (MQ=255)
                                                     gTAATGAAAGAACCCAAATCAATTCACAGTTCACAACTTATCCTTTTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTg                                                                                              <  1:1825892/150‑1 (MQ=255)
                                                      tAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGt                                                                                             <  2:253653/150‑1 (MQ=255)
                                                         tGAAAGAACCCAAATCAATTCGCCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGATGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTg                                                                                          >  1:875268/1‑150 (MQ=255)
                                                          taaaGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTgg                                                                                         <  2:1815082/149‑1 (MQ=255)
                                                          gAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTgg                                                                                         >  2:1647598/1‑150 (MQ=255)
                                                            aaGAACCAAAATCAATTCCCCGTTCACAACTTATCCTTGTTAATTGAGTGGGATGACTTGAATCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTc                                                                                       <  1:337417/150‑1 (MQ=255)
                                                               aaCCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAAATGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTcaca                                                                                    >  1:946857/1‑150 (MQ=255)
                                                                aCCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAg                                                                                   <  2:772989/150‑1 (MQ=255)
                                                                aCCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAg                                                                                   <  1:787690/150‑1 (MQ=255)
                                                                aCCCAAATCAATTCACCGTTCACAACTAATCCTTGTTATTTGCGGGGGATGACTTGATTCTGCCATAACAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGGCACAg                                                                                   <  1:1012668/150‑1 (MQ=255)
                                                                 cccAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGt                                                                                  <  1:686719/150‑1 (MQ=255)
                                                                 cccAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGt                                                                                  >  1:590008/1‑150 (MQ=255)
                                                                 cccAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGt                                                                                  >  2:330368/1‑150 (MQ=255)
                                                                  ccAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTa                                                                                 >  2:725137/1‑150 (MQ=255)
                                                                    aaaTCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAAt                                                                               >  1:1105626/1‑150 (MQ=255)
                                                                     aaTCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAGTCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAAtt                                                                              <  2:203998/150‑1 (MQ=255)
                                                                        cAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGc                                                                           <  1:547886/150‑1 (MQ=255)
                                                                        cAATTCACCGTTCACAACTTAT‑CATGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCt                                                                          <  2:654440/150‑1 (MQ=255)
                                                                          aTTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTc                                                                         >  2:425887/1‑150 (MQ=255)
                                                                          aTTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTc                                                                         <  1:1868278/150‑1 (MQ=255)
                                                                          aTTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTc                                                                         >  1:1600145/1‑150 (MQ=255)
                                                                           ttCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTcc                                                                        >  1:19485/1‑150 (MQ=255)
                                                                             cACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAg                                                                      >  2:1646189/1‑150 (MQ=255)
                                                                             cACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGACATTTGGCTGTTTGGTCACAGTAATTTGCTCCAg                                                                      >  2:1903042/1‑150 (MQ=255)
                                                                             cACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACATGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAg                                                                      <  2:520422/150‑1 (MQ=255)
                                                                                    cacaACTTATCCTTGTTAATTGCGGGGGATGACTTGATTCTGCCAAAAAAAAGCCCCGACGAGACGGGGCTAAATCTTGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTgg                                                               <  2:1877299/150‑1 (MQ=255)
                                                                                    cacaACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTgg                                                               >  2:93626/1‑150 (MQ=255)
                                                                                     acaACTTATCCTTGTTAATTGCGGTGGATGACTTGATTCTGCCATAAAAACGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACAGAAAAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTgga                                                              <  2:1848535/150‑1 (MQ=255)
                                                                                     acaACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTgga                                                              <  1:670555/150‑1 (MQ=255)
                                                                                       aaCTTATCCTTGTTAATTGAGGGGGAGGACTTGATTCTGCCAAAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTggagg                                                            <  2:585122/150‑1 (MQ=255)
                                                                                        aCTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTggagga                                                           >  2:983858/1‑150 (MQ=255)
                                                                                        aCTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTggagga                                                           <  1:1529560/150‑1 (MQ=255)
                                                                                         attATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGAc                                                          <  1:1428100/149‑1 (MQ=255)
                                                                                           tATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCa                                                        >  2:1455870/1‑150 (MQ=255)
                                                                                              ccTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCTCAGTAATTTGCTCCAGACTGTGGAGGACCaact                                                     >  2:1364793/1‑148 (MQ=255)
                                                                                                     aaTTGAGGGGGATGACTTGATTCTGCCATAAAAAACCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTc                                              <  2:417580/150‑1 (MQ=255)
                                                                                                         gctggggATTACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCTCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTc                                          <  1:941208/147‑1 (MQ=255)
                                                                                                         gAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTc                                          >  2:456581/1‑150 (MQ=255)
                                                                                                         gAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTc                                          >  1:764086/1‑150 (MQ=255)
                                                                                                                ttGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGcgcg                                   <  2:1483327/149‑1 (MQ=255)
                                                                                                                 tGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCgg                                  <  2:1369704/150‑1 (MQ=255)
                                                                                                                  gACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCggg                                 <  1:963441/150‑1 (MQ=255)
                                                                                                                   aCTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCCGGCGCgggg                                >  1:1229418/1‑150 (MQ=255)
                                                                                                                   aCTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCgggg                                <  2:137023/150‑1 (MQ=255)
                                                                                                                   aCTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCgggg                                <  1:1511151/150‑1 (MQ=255)
                                                                                                                    cTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCACATCGACCTACTTCAGGCGCGGGGt                               >  2:1483816/1‑150 (MQ=255)
                                                                                                                    cTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGt                               >  1:113205/1‑150 (MQ=255)
                                                                                                                      tGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGGAt                             >  2:144032/1‑150 (MQ=255)
                                                                                                                       taTTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATc                            <  2:1834282/149‑1 (MQ=255)
                                                                                                                         ttCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTg                          <  1:1199043/150‑1 (MQ=255)
                                                                                                                         ttCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGAGCAGGTGTGTCACTGAATAATAACATCGCCATTTGGCTGTTTGTTGACTGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGTTATCTg                          <  1:288519/150‑1 (MQ=255)
                                                                                                                           cTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGcc                        <  1:1691663/150‑1 (MQ=255)
                                                                                                                            tGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCg                       <  1:1579424/150‑1 (MQ=255)
                                                                                                                             gCCATCAAAACGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCgg                      <  2:158293/150‑1 (MQ=255)
                                                                                                                             gCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCgg                      >  2:1560134/1‑150 (MQ=255)
                                                                                                                              ccATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGa                     >  1:712900/1‑150 (MQ=255)
                                                                                                                                 tgatttaGCCCCGTCTCGTCGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCg                  >  2:1045494/7‑150 (MQ=255)
                                                                                                                                  gatttaGCCCCGTCTCGTCGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGCCCTCATTCAGGCGCGGGGTATCTGCCGGAGCGt                 >  2:788937/6‑150 (MQ=255)
                                                                                                                                    aaaaGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTa               <  1:1680578/150‑1 (MQ=255)
                                                                                                                                    aaaaGCCACGACGAGACGGGGCTAAATCATGCTCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTAGCTGCCGGAGCGTTa               <  1:322295/150‑1 (MQ=255)
                                                                                                                                      taGCCCCGTCTCGTCGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAAc             >  1:1270490/2‑150 (MQ=255)
                                                                                                                                        gCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCTCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAACCg           <  1:1692394/150‑1 (MQ=255)
                                                                                                                                             gacgaGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAACCGCAatg      >  1:1373176/1‑150 (MQ=255)
                                                                                                                                             gacgaGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGGATCTGCCGGAGCGTTAACCGCAatg      <  1:60274/150‑1 (MQ=255)
                                                                                                                                             gacgaGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGGTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAACCGCAatg      <  1:1152953/150‑1 (MQ=255)
                                                                                                                                               cgaGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAACCGCAatgac    <  2:533075/150‑1 (MQ=255)
                                                                                                                                                gagaCGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAACCGCAatgaca   <  1:1081996/150‑1 (MQ=255)
                                                                                                                                                 agaCGTGGCTAAATCATGATCTGGGGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCTCAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGTCGCGGGGTATCTGCCGGAGCGTTAACCGCAatgacat  <  2:318473/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GACCGCATCCATACGCACTCTGCCCGTCGCAAACAAGACGATGGCAACGGCCAGTAATGAAAGAACCCAAATCAATTCACCGTTCACAACTTATCCTTGTTAATTGAGGGGGATGACTTGATTCTGCCATAAAAAAGCCCCGACGAGACGGGGCTAAATCATGATCAGGTGTTTCACTGAATAATAACATCGCCATTTGGCTGTTTGGTCACAGTAATTTGCTCCAGACTGTGGAGGACCAAATCGACCTCATTCAGGCGCGGGGTATCTGCCGGAGCGTTAACCGCAATGACAT  >  NC_000913/2411266‑2411560

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑