Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,464,228 C→A 7.6% intergenic (+158/+24) yfdF → / ← mlaA uncharacterized protein/ABC transporter maintaining OM lipid asymmetry, OM lipoprotein component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,464,2280CA 7.6% 330.3 / 8.9 117intergenic (+158/+24)yfdF/mlaAuncharacterized protein/ABC transporter maintaining OM lipid asymmetry, OM lipoprotein component
Reads supporting (aligned to +/- strand):  ref base C (44/62);  new base A (6/5);  total (50/67)
Fisher's exact test for biased strand distribution p-value = 5.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 117 total aligned reads displayed.

CAGTTTTTTGATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCACCATAAT  >  NC_000913/2464082‑2464377
                                                                                                                                                  |                                                                                                                                                     
cAGTTTTTTGATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGATTTTTGCGAc                                                                                                                                                    >  1:1363886/1‑150 (MQ=255)
   ttttttGATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCa                                                                                                                                                 <  1:467351/150‑1 (MQ=255)
   ttttttGATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCa                                                                                                                                                 <  1:1346492/150‑1 (MQ=255)
     ttttGATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCAcc                                                                                                                                               <  2:879072/150‑1 (MQ=255)
        tGATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGAGACTCACCttt                                                                                                                                            >  1:1877974/1‑150 (MQ=255)
         gATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCtttt                                                                                                                                           >  2:227989/1‑150 (MQ=255)
         gATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGGTTATCTCCTTTTACTTGTGGTAATTTAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCtttt                                                                                                                                           >  1:944533/1‑150 (MQ=255)
          aTTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCttttt                                                                                                                                          <  2:1229640/150‑1 (MQ=255)
           tttttCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCtttttt                                                                                                                                         >  2:1912205/1‑150 (MQ=255)
           tttttCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCtttttt                                                                                                                                         >  2:632611/1‑150 (MQ=255)
           tttttCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTCGCAAAACTCACCtttttt                                                                                                                                         <  2:1474515/150‑1 (MQ=255)
           tttttCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTATTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTAATTGAGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCattttt                                                                                                                                         >  2:1527082/1‑150 (MQ=255)
            ttttCTACAGCTGAATTTCGGAAATATTCAGTGACGAACTTCTGTATTTTTAGATAATTACGTCTAGGTCATTGTCGGTTTATCTGCTTTTTCTTGTGGTAATTTAGGGATAACAACAAATAAGGAGAGTCGCTAAACTCACCttttttg                                                                                                                                        <  2:739470/150‑2 (MQ=255)
            ttttCAACAGCTGAATATCGGAAATAGTCCGTGACGAACACCAGTATTTTAACATAATTACGTCTAGGGCATTGGCGGTTTAGCTCCTTTTACTTGTGGTACTTGAGGGATAACAACAAAAAAGGTGAGTCGCAAAACTCACCttttttg                                                                                                                                        <  1:161858/150‑2 (MQ=255)
             tttCAACAGCTGAATATCGTAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTCGCAAAACTCACCttttttgt                                                                                                                                       <  1:426747/150‑3 (MQ=255)
             tttCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAATAAAAAAGGTGAGTCGCAAAACTCACCttttttgt                                                                                                                                       <  1:920713/150‑3 (MQ=38)
                cAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTg                                                                                                                                    <  1:1418705/150‑1 (MQ=255)
                cAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTg                                                                                                                                    <  2:752432/150‑1 (MQ=255)
                  aCAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGtt                                                                                                                                  <  2:568862/150‑1 (MQ=255)
                   aaGCTGAATATCGGTAATATTCAGTGACGAACATCATTATTTTTACATATTTACGTCTAGGTCATTGTCGTTGTATCTCCTTTTACTTGTGGTAATTGAGGGATAACTACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGttt                                                                                                                                 <  2:127716/149‑1 (MQ=255)
                    atcTGAATATCGGAAATAGTCATTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTc                                                                                                                                <  2:23496/148‑1 (MQ=255)
                    aGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTTGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTc                                                                                                                                <  1:1285339/150‑1 (MQ=255)
                     gCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCt                                                                                                                               <  1:1018999/150‑1 (MQ=255)
                       tGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTTCATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTa                                                                                                                             <  2:1269685/150‑1 (MQ=255)
                        taaTATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAAATACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTAt                                                                                                                            <  1:1836577/149‑1 (MQ=255)
                          atatCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTc                                                                                                                          <  1:244750/150‑1 (MQ=255)
                          atatCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTc                                                                                                                          <  2:1286180/150‑1 (MQ=255)
                           aatCGGAAAAAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCAAGGTCATTGTCGGTTTATCTCCATTTACATGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCa                                                                                                                         <  1:61044/149‑1 (MQ=255)
                            atCTGAAATAGTCAGTGAAGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAg                                                                                                                        <  2:1035459/150‑1 (MQ=255)
                            atCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAg                                                                                                                        <  1:414266/150‑1 (MQ=255)
                            atCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAg                                                                                                                        >  1:1212062/1‑150 (MQ=255)
                               ggAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAAt                                                                                                                     >  2:748748/1‑150 (MQ=255)
                               ggAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTATTGAGACTCACCTTTTTTATTTGTTTCTTATTCATAAt                                                                                                                     >  1:218402/1‑150 (MQ=255)
                                gAAATAGTCAGTTACGAACATCAGTATTTTTACAAAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTATTTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATc                                                                                                                    <  1:662841/150‑1 (MQ=255)
                                   aTAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCATTTTACTTGGGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAat                                                                                                                 <  2:1730895/150‑1 (MQ=255)
                                     aGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAatat                                                                                                               >  1:1517283/1‑150 (MQ=255)
                                        cAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCtt                                                                                                            >  1:1415013/1‑150 (MQ=255)
                                        cAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGAGACTCACCATTTTTATTTGTTTATTATTAATAATAAATATCtt                                                                                                            >  1:1663392/1‑150 (MQ=255)
                                         actGACGAAAATCAGTATTTTTACATTATTACGTCTAGGTCATTGTCGGTTTATATCCTTTTACTTGTGGTAATTGAGCTATAACCACAAAAAAGTGGAGTTTAGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAAGCAATATCttt                                                                                                           <  1:605459/148‑1 (MQ=255)
                                         aGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCttt                                                                                                           <  1:1315045/150‑1 (MQ=255)
                                            gaggAACATCAGTATTTTTACATTATTACGTCTATGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGTTAACAACAATATAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTaa                                                                                                        <  1:852545/147‑1 (MQ=255)
                                             aCGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTaaa                                                                                                       <  2:575054/150‑1 (MQ=255)
                                                   aTCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTAATTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATATTTTTAATAATcc                                                                                                 >  2:412946/1‑150 (MQ=255)
                                                    tCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCt                                                                                                <  1:789089/150‑1 (MQ=255)
                                                    tCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCAACTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAatcatcat                                                                                                >  1:1777308/1‑148 (MQ=255)
                                                     cAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTg                                                                                               >  2:683331/1‑150 (MQ=255)
                                                       gTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGaa                                                                                             >  2:506455/1‑150 (MQ=255)
                                                            tttACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCt                                                                                        <  2:821882/150‑1 (MQ=255)
                                                              tACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTtg                                                                                      <  1:1561828/150‑1 (MQ=255)
                                                               aCATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTtgt                                                                                     >  2:1055943/1‑150 (MQ=255)
                                                               aCATAATTACGTCTAGGGCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTtgt                                                                                     <  2:1411141/150‑1 (MQ=255)
                                                                cATAATTACGTCTAGGTCATTGTCGATTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTtgtg                                                                                    <  2:1528283/150‑1 (MQ=255)
                                                                 aTAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGTATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGc                                                                                   <  1:1165995/150‑1 (MQ=255)
                                                                 aTAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGc                                                                                   <  1:1586051/150‑1 (MQ=255)
                                                                      tACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCg                                                                              <  2:1176144/150‑1 (MQ=255)
                                                                       aCGTCTAGGTCATTGTCGGATTATCTACTTTAACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCATTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCgg                                                                             >  2:903155/1‑150 (MQ=255)
                                                                        cGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGAGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGa                                                                            >  1:52318/1‑150 (MQ=255)
                                                                               gTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCt                                                                     <  1:104666/150‑1 (MQ=255)
                                                                                    tGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGt                                                                <  2:677284/150‑1 (MQ=255)
                                                                                     gtgggTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAATGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGtt                                                               <  2:1812168/147‑1 (MQ=255)
                                                                                     gTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGtt                                                               <  2:1865756/150‑1 (MQ=255)
                                                                                       cGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTg                                                             >  2:45487/1‑150 (MQ=255)
                                                                                        ggTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGa                                                            <  1:1233390/150‑1 (MQ=255)
                                                                                         gTTTATCTCCTTTTACTTGTTGTAATTGAGGGATAAAAACTAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTTTTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAg                                                           <  1:1912205/150‑1 (MQ=255)
                                                                                         gTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAg                                                           <  1:632611/150‑1 (MQ=255)
                                                                                             aTCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTcg                                                       <  2:392709/150‑1 (MQ=255)
                                                                                             aTCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTcg                                                       >  1:1299657/1‑150 (MQ=255)
                                                                                             aTCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTcg                                                       >  2:446219/1‑150 (MQ=255)
                                                                                              tctcGTTTTACTTGTGGTAATTGAGTGAAAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTcgc                                                      <  2:102551/150‑1 (MQ=255)
                                                                                                 ccTTTTACTTGTGGTGATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTctccgc                                                   >  2:461776/1‑150 (MQ=255)
                                                                                                 ccTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTcgccgc                                                   >  2:1763474/1‑150 (MQ=255)
                                                                                                   ttttACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTCAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCa                                                 <  2:609211/150‑1 (MQ=255)
                                                                                                   ttttACTTGTGGTAATTGAGGGATAACAAAAAAAAATGTGAGTTTTTAGACTCACCTTTTTTATTTGTTTATTATTAAGAATCAATATATTTTAAATAATAAATAAAATCTTTTTCGTTAAGAATTTAATGCGGTTTGAGTTAGGCGCCa                                                 >  2:1611361/1‑150 (MQ=255)
                                                                                                      tACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTa                                              >  1:1586032/1‑150 (MQ=255)
                                                                                                      tACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTa                                              >  1:85406/1‑150 (MQ=255)
                                                                                                      tACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTa                                              <  1:909549/150‑1 (MQ=255)
                                                                                                             ggTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGa                                       >  1:630832/1‑150 (MQ=255)
                                                                                                             ggTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCAGCCATTAGCGATGa                                       >  1:420036/1‑150 (MQ=255)
                                                                                                                aaTTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAAt                                    >  2:1035243/1‑150 (MQ=255)
                                                                                                                   tGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCAt                                 <  2:881732/150‑1 (MQ=255)
                                                                                                                   tGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGGTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCAt                                 <  2:1761357/150‑1 (MQ=255)
                                                                                                                    taGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATg                                <  2:1610658/149‑1 (MQ=255)
                                                                                                                        gATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTGGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGc                            >  2:695550/1‑150 (MQ=255)
                                                                                                                        gATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGc                            >  2:1532462/1‑150 (MQ=255)
                                                                                                                        gATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGc                            >  2:1344122/1‑150 (MQ=255)
                                                                                                                             caacaaAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGaa                       >  1:1820183/1‑150 (MQ=255)
                                                                                                                              aataaaaaaGGTGAGTCGCAAAACTCACCTTTTTTATTTGTTTCTAATTCAGAAGAAAATTGTTTTAATTAATCCTGATTGGCTTGTGCGTAGGGATTTACATGCGTTTTAATTTCGCCGCCATATGGGATGAAATCTTGACGGGGGAAg                      >  2:172855/4‑150 (MQ=255)
                                                                                                                                caaAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTa                    >  1:1854875/1‑150 (MQ=255)
                                                                                                                                   aaaaGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGc                 <  1:449849/150‑1 (MQ=255)
                                                                                                                                         tGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTGAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTcgcg           <  2:581656/150‑1 (MQ=255)
                                                                                                                                          gAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCgcgc          <  2:218402/150‑1 (MQ=255)
                                                                                                                                           agatttGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCa         <  1:1166420/147‑1 (MQ=255)
                                                                                                                                           aGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTATCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCa         <  1:1898697/150‑1 (MQ=255)
                                                                                                                                            gTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCAc        >  2:448151/1‑150 (MQ=255)
                                                                                                                                             ttttGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCAcc       <  1:442338/150‑1 (MQ=255)
                                                                                                                                              tttGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCACCa      >  2:1237095/1‑150 (MQ=255)
                                                                                                                                              tttGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCACCa      >  2:903435/1‑150 (MQ=255)
                                                                                                                                                 gCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCACCataa   <  1:227989/150‑1 (MQ=255)
                                                                                                                                                 gCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCACCataa   <  1:1297876/150‑1 (MQ=255)
                                                                                                                                                 gCGACTCACCTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCACCataat  >  2:1001377/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
CAGTTTTTTGATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCACCATAAT  >  NC_000913/2464082‑2464377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: