Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,470,482 C→A 7.7% intergenic (+20/‑333) gtrS → / → tfaS serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage/pseudogene, CPS‑53 (KpLE1) prophage; tail fiber assembly protein fragment;Phage or Prophage Related

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,470,4820CA 7.7% 373.7 / 9.2 139intergenic (+20/‑333)gtrS/tfaSserotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage/pseudogene, CPS‑53 (KpLE1) prophage; tail fiber assembly protein fragment;Phage or Prophage Related
Reads supporting (aligned to +/- strand):  ref base C (52/73);  new base A (10/4);  total (62/77)
Fisher's exact test for biased strand distribution p-value = 4.63e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 139 total aligned reads displayed.

TAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCGTTGTTTATTGTAATATCTCCTCTATCTGCAGACGATAACTTAAATG  >  NC_000913/2470336‑2470630
                                                                                                                                                  |                                                                                                                                                    
taGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCCAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATg                                                                                                                                                   <  2:44711/150‑1 (MQ=255)
taGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCCCGCCAATCATg                                                                                                                                                   >  2:368115/1‑150 (MQ=255)
 agagATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATgg                                                                                                                                                  <  1:752258/150‑1 (MQ=255)
 agagATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATgg                                                                                                                                                  >  2:31360/1‑150 (MQ=255)
  tagaTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGgc                                                                                                                                                 <  2:798579/149‑1 (MQ=255)
    taTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGgcgc                                                                                                                                               <  1:1011881/149‑1 (MQ=255)
       ttAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGca                                                                                                                                            <  2:1093028/150‑1 (MQ=255)
       ttAAGCAAGCATTTATNTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATAAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATAATGGCGCGca                                                                                                                                            >  1:598551/1‑150 (MQ=255)
        tAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCATGATTGGCGCGcaa                                                                                                                                           <  2:1577318/150‑2 (MQ=255)
        tAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATAAATACAATATCGATGTAAAGCACTAAGTTTAAATTTCGCGCCAATAATGgcgcgaaa                                                                                                                                           >  2:1438040/1‑147 (MQ=255)
               gcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCtat                                                                                                                                    <  1:280700/150‑1 (MQ=255)
                caTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCtata                                                                                                                                   >  2:772737/1‑150 (MQ=255)
                caTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCtaaa                                                                                                                                   >  2:365784/1‑148 (MQ=255)
                  tttatttGGAATGTATTTTTCGTTCGACCTTTGGTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCAATAAGTTTAAATTGCGCGCGAATAATGGCTCGCACAAGCTataaa                                                                                                                                 >  1:820820/1‑149 (MQ=255)
                  tttatttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTataat                                                                                                                                 <  1:978278/150‑1 (MQ=255)
                    tatttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATAc                                                                                                                               >  2:1851636/1‑150 (MQ=255)
                        tGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAAc                                                                                                                           <  2:93484/150‑1 (MQ=255)
                         ggAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAAcc                                                                                                                          >  2:1051620/1‑150 (MQ=255)
                         ggAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAAcc                                                                                                                          >  2:799099/1‑150 (MQ=255)
                              ttATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCTATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAAtt                                                                                                                     <  1:238019/149‑1 (MQ=255)
                              ttATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAAtt                                                                                                                     <  2:482904/149‑1 (MQ=255)
                               tATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAAttt                                                                                                                    <  2:31444/150‑1 (MQ=255)
                                aTTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTtc                                                                                                                   <  1:1687351/150‑1 (MQ=255)
                                aTTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAAATCAAAGAAATAAAGACAATAGCGATGTAAAGCACTAAATTTAAATTTCGCGCCAATAATGGCGCGCACAAGCTATAATACCAACATAAttta                                                                                                                   >  1:855577/1‑149 (MQ=255)
                                  ttttCGTTCGACCTTTGATTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAATAAATCAAGACAATAGCTATGTAAAGAACTAATTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTtctc                                                                                                                 <  1:1438040/150‑1 (MQ=255)
                                   tttCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTctcc                                                                                                                <  1:360133/150‑1 (MQ=255)
                                    ttcgttcgACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTctcct                                                                                                               <  2:1483680/150‑1 (MQ=255)
                                      cgttcgACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTctcctcc                                                                                                             <  2:675303/150‑1 (MQ=255)
                                      cgttcgACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTctcctcc                                                                                                             <  2:1532545/150‑1 (MQ=255)
                                          cgACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCtt                                                                                                         >  2:1351230/1‑150 (MQ=255)
                                           gACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTTCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTa                                                                                                        >  2:398580/1‑150 (MQ=255)
                                             ccTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTTCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTATACTCCTCTTaga                                                                                                      >  1:957382/1‑150 (MQ=255)
                                              cTTTGCTTATACATAACAGTCGTTTTTTTTATTTTTAAAATAAATAAAATAAAAAAAAACAAAATCCTATTAAAGAAATAAGTTTAAAATTAGCGCCAAACATTGCGCCCACCAGCTATAAAAACAACAAAATTTATCCTACAATTagat                                                                                                     >  2:1562869/1‑149 (MQ=255)
                                               tttGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCTATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGt                                                                                                    <  2:1594871/150‑1 (MQ=255)
                                               tttGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGt                                                                                                    <  1:406590/150‑1 (MQ=255)
                                                  gCTTATACTTAACAGTCGTTTTTTTTATTTATAAAATAATTAAAAGAAATAAAGACAATAGCGATGTAAAGCAATAAGTTTAAATTTCGCGCCAAAAAATGCCCGCCCAAGCTATAATACCCACCTAATTTCTCATCCTCTTATAGTGaa                                                                                                 >  1:749640/1‑149 (MQ=255)
                                                    ttATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACta                                                                                               <  1:834124/150‑1 (MQ=255)
                                                    ttATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACta                                                                                               <  1:595302/150‑1 (MQ=255)
                                                     tataCTAAACAGTCTTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACtat                                                                                              <  2:623102/150‑1 (MQ=255)
                                                      ataCATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACtata                                                                                             <  2:1172551/150‑1 (MQ=255)
                                                        aCATAACAGTCGTGTTTTTAATTTATAAAATAATTCAAAGAAATAAAGAAAAAATCCAAGTAAAGCAATAAGTTTAAATTGCGCGCCAAACAATGCGCCCACAAGCTAAAATACAAACCTAATTTCTCCTCCTCATAGAGTGAatatata                                                                                           >  2:1866500/1‑149 (MQ=255)
                                                         aaTAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATAtct                                                                                          <  2:690340/149‑1 (MQ=255)
                                                          aTAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATAtctc                                                                                         >  1:527708/1‑150 (MQ=255)
                                                           tAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTcc                                                                                        >  2:253433/1‑150 (MQ=255)
                                                           tAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTcc                                                                                        <  2:746643/150‑1 (MQ=255)
                                                            aaCAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCt                                                                                       <  2:1831049/150‑1 (MQ=255)
                                                            aaCAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCt                                                                                       <  1:1330123/150‑1 (MQ=255)
                                                              cAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTTCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGa                                                                                     >  1:1623050/1‑150 (MQ=255)
                                                              cAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCAAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGa                                                                                     >  1:1039496/1‑150 (MQ=255)
                                                                gTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATa                                                                                   <  1:1751540/150‑1 (MQ=255)
                                                                       ttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATAACAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGc                                                                            >  2:634614/1‑150 (MQ=255)
                                                                        tttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCg                                                                           >  2:1005427/1‑150 (MQ=255)
                                                                         ttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCgg                                                                          <  1:647287/150‑1 (MQ=255)
                                                                           aaTTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTa                                                                        <  2:917864/150‑1 (MQ=255)
                                                                              ttattaAATAATTCAATGAAATCAAGACAATAGCGATGTAAAGCACTATTTTTAAATTGCGCGCAAATCTTGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTg                                                                     <  1:956965/150‑1 (MQ=255)
                                                                                ataAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGAc                                                                   <  1:204768/150‑1 (MQ=255)
                                                                                        atTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAAt                                                           >  2:459132/1‑150 (MQ=255)
                                                                                          tCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATAGCGCGCACAAGCTATAATACCAACCTAATTTCTACTCCTCTTAGAGTGACTATATCTCCTCATAGAATTGCGGTATTGACTATCAAATga                                                         >  1:1172706/1‑149 (MQ=255)
                                                                                           cAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGCCTATCAAATGcc                                                        >  2:128256/1‑150 (MQ=255)
                                                                                           cAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGcc                                                        >  1:555744/1‑150 (MQ=255)
                                                                                           cAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCACCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGcc                                                        >  1:161456/1‑150 (MQ=255)
                                                                                            agaGAAATCAAGACAATTGGGCTGTAAGGCACTAAGTTTTAATTGCGCGCCAATCATGGCGCGCACACGCTATATTACCAACCTACTTTCTCCTCCTCATAGAGTGACGATATGTCCTGATAGAATTGCGGTATTGACGATCAAATGccc                                                       >  2:1807192/3‑150 (MQ=255)
                                                                                             aaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCt                                                      >  1:1303606/1‑150 (MQ=255)
                                                                                                  aTCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTc                                                 >  2:430569/1‑150 (MQ=255)
                                                                                                  aTCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTc                                                 >  2:615750/1‑150 (MQ=255)
                                                                                                     aaGACAATATCGATGTAAAGCAATAAGTTTAAATTGCGCTCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCgtt                                              <  1:1844286/150‑1 (MQ=255)
                                                                                                     aaGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCgtt                                              <  1:1758153/150‑1 (MQ=255)
                                                                                                      aGAAAATAGCTATGTAAAGCAATAATTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCgttg                                             <  1:875824/150‑1 (MQ=255)
                                                                                                       gACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCTCAAATCATGGAGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGTATTGCGGTATTGTCTATCAAATGCCCTGATTCgttgt                                            <  1:1164858/150‑1 (MQ=255)
                                                                                                        aCAATAGCGATGTACAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCgttgtt                                           <  2:346711/150‑1 (MQ=255)
                                                                                                         cAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCGTTGttt                                          >  2:1439360/1‑150 (MQ=255)
                                                                                                         cAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCGTTGttt                                          <  1:1544292/150‑1 (MQ=255)
                                                                                                         cAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCGTTGttt                                          <  2:764607/150‑1 (MQ=255)
                                                                                                           aTAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCGTTGTTTAt                                        >  1:1216335/1‑150 (MQ=255)
                                                                                                             aGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCGTTGTTTATTg                                      <  2:129172/150‑1 (MQ=255)
                                                                                                             aGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCGTTGTTTATTg                                      <  2:493900/150‑1 (MQ=255)
                                                                                                                   gTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCGTTGTTTATTGTAatat                                >  1:1266835/1‑150 (MQ=255)
                                                                                                                    tAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCGTTGTTTATTGTAATAtc                               >  1:1510647/1‑150 (MQ=255)
                                                                                                                    tAAAGAACTAAGTTTAAATTGCGCGCCAATCATCGCGCGCACAAGCTATAATAACAACCTAATTTCTCCTCCTCTTAGTGTGACTATATCTCCTTATAGAATTGCGGTATTGACTATCAAATGGCCTGATTCGTTGTTTATTGTAATAtc                               <  1:1911047/150‑1 (MQ=255)
                                                                                                                     aaaGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCGTTGTTTATTGTAATAtct                              >  2:824503/1‑150 (MQ=255)
                                                                                                                       aGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCGTTGTTTATTGTAATATctcc                            <  1:1854821/150‑1 (MQ=255)
                                                                                                                        gCACTAATTTTAAATTGCGCGCCATTCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTCTTGACTATCAAATTCCCTGATTCGTTGTTTATTGTAATATctcct                           <  1:1866500/150‑1 (MQ=255)
                                                                                                                             aaGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCGTTGTTTATTGTAATATCTCCTCTATc                      <  1:1396283/150‑1 (MQ=255)
                                                                                                                               gTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCGTTGTTTATTGTAATATCTCCTCTATCTg                    >  1:398640/1‑150 (MQ=255)
                                                                                                                               gTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCATAGAGTGACTATATCTCCTGTAAGAATTGCGGTATTGAATATCAAATGCCCTGATTCGTTGTTTATTGTAATATCTCCTCTATCTg                    <  2:1126477/150‑1 (MQ=255)
                                                                                                                                tttAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCGTTGTTTATTGTAATATCTCCTCTATCTGc                   <  1:473695/150‑1 (MQ=255)
                                                                                                                                 ttAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCGTTGTTTATTGTAATATCTCCTCTATCTGCa                  <  2:119882/150‑1 (MQ=255)
                                                                                                                                   aaaTTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCGTTGTTTATTGTAATATCTCCTCTATCTGCAGa                <  1:104571/150‑1 (MQ=255)
                                                                                                                                    aaTTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCGTTGTTTATTGTAATATCTCCTCTATCTGCAGAc               >  2:343319/1‑150 (MQ=255)
                                                                                                                                    aaTTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCGTTGTTTATTGTAATATCTCCTCTATCTGCAGAc               <  1:772737/150‑1 (MQ=255)
                                                                                                                                     aTTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAAATGCGGTATTGACTATCAAATGCCCTGATTCGTTGTTTATTGTAATATCTCCTATATCTGCAGACg              >  1:937098/1‑150 (MQ=255)
                                                                                                                                      ttGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCGTTGTTTATTGTAATATCTCCTCTATCTGCAGACGa             >  1:1526174/1‑150 (MQ=255)
                                                                                                                                      gtGCGCGCCATGATTGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCGTTGTTTTTTGTAATATCTCCTCTATCTGCAGACGa             >  2:845605/2‑150 (MQ=255)
                                                                                                                                        gcgcgcCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCGTTGTTTATTGTAATATCTCCTCTATCTGCAGACGATa           >  2:1837185/1‑150 (MQ=255)
                                                                                                                                          gcgcCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCATCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCGTTGTTTATTGTAATATCTCCTCTATCTGCAGACGATAAc         <  1:1248230/150‑1 (MQ=255)
                                                                                                                                           cgcCAATCTTGGCGCGCACAAGCTATAATACCAACCTAATTTCTGCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCGTTGTTTTTTGTAATATCTCCTCTATCTGCAGACGATAACt        <  2:1530791/150‑1 (MQ=255)
                                                                                                                                           cgcCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCGTTGTTTATTGTAATATCTCCTCTATCTGCAGACGATAACt        >  1:1252460/1‑150 (MQ=255)
                                                                                                                                               aaTCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATATAATTGCGGTATTGACTATCAAATGCCCTGAGTCGTTGTTGATTGTAATATCTCCGCTATCTGCAGACGAGAACTTaaa    >  2:188738/1‑150 (MQ=255)
                                                                                                                                                aTCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCGTTGTTTATTGTAATATCTCCTCTATCTGCAGACGATAACTTAAAt   <  2:855577/150‑1 (MQ=255)
                                                                                                                                                 tCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCGTTGTTTATTGTAATATCTCCTCTATCTGCAGACGATAACTTAAATg  <  2:1623050/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
TAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCGTTGTTTATTGTAATATCTCCTCTATCTGCAGACGATAACTTAAATG  >  NC_000913/2470336‑2470630

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: