Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,525,907 G→A 5.7% intergenic (+16/+23) yfeN → / ← yfeR putative outer membrane protein/transcriptional regulator of yefH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,525,9070GA 5.7% 446.2 / 10.0 143intergenic (+16/+23)yfeN/yfeRputative outer membrane protein/transcriptional regulator of yefH
Reads supporting (aligned to +/- strand):  ref base G (75/58);  new base A (3/5);  total (79/64)
Fisher's exact test for biased strand distribution p-value = 4.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.44e-01

Only 100 of 145 total aligned reads displayed.

TGGCGCAGTTGCACTGTGGTGGAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATC  >  NC_000913/2525759‑2526053
                                                                                                                                                    |                                                                                                                                                  
tGGCGAAGTTGCACTGTGGTGGAATGCAAAATCACAAATTAATACGGGGATTCATTATCTATTTTCGGATGACAAACTGGGCTAAGATTTTTACAAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGc                                                                                                                                                   <  1:1214829/150‑1 (MQ=255)
       gTTGCACTGTGGTGGAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGc                                                                                                                                            <  2:396155/150‑1 (MQ=255)
       gTTGCACTGTGGTGGAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGc                                                                                                                                            <  1:1890018/150‑1 (MQ=255)
          gCACTGTGGTGGAATGCAACATCACACATTACTACGGGGCTTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATAACTGCCGGGGCTCCCGGCAGt                                                                                                                                         <  1:10254/150‑1 (MQ=255)
          gCACTGTGGTGGAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGt                                                                                                                                         <  1:35133/150‑1 (MQ=255)
          gCACTGTGGTGGAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGAGCCCCGGCAGt                                                                                                                                         <  2:414826/150‑1 (MQ=255)
             ctctggtggAATGCAACATCACACATTACTACGGGGAATCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACAGCCGGCCCTCCCGGCAGTATg                                                                                                                                      >  2:1259476/4‑150 (MQ=255)
             cTGTGGTGGAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCAGGGGCTCCCGGCAGTATg                                                                                                                                      >  1:809296/1‑150 (MQ=255)
              tgtgGTGGAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGAGCCCCGGCAGTaatc                                                                                                                                     <  1:1499059/150‑4 (MQ=255)
              tgtgGTGGAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGAGCCCCGGCAGTaatc                                                                                                                                     <  1:849823/150‑4 (MQ=255)
                 ggtggAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGAAGACAAACTGGGCGAAGATTTTTACCAGCATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGAGCCCCGGCAGTaatcaat                                                                                                                                  <  1:1729946/150‑7 (MQ=255)
                  gtggAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTc                                                                                                                                 >  2:1322821/1‑150 (MQ=255)
                   tggAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCa                                                                                                                                >  2:1588130/1‑150 (MQ=255)
                   tggAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCa                                                                                                                                >  2:1233016/1‑150 (MQ=255)
                    ggAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTTGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCAc                                                                                                                               <  2:48879/150‑1 (MQ=255)
                    ggAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCAc                                                                                                                               <  2:461188/150‑1 (MQ=255)
                       aTGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCActct                                                                                                                            <  1:190454/150‑1 (MQ=255)
                        tGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCta                                                                                                                           <  2:1164487/150‑1 (MQ=255)
                        tGCAACAACACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCta                                                                                                                           <  1:784926/150‑1 (MQ=255)
                         gCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCtat                                                                                                                          >  1:43280/1‑150 (MQ=255)
                          cAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCtata                                                                                                                         <  2:1886692/150‑1 (MQ=255)
                          cAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCtata                                                                                                                         >  2:1598192/1‑150 (MQ=255)
                             cATCACACATTACTACTGGGATTCAGTATCTATATGCTGATGACAAACTTGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCTTCAAATTTAACTTGTGATTGATTACTGCAGGGGCTCCCGGAAGTATGAATTCACTCTATATCt                                                                                                                      <  2:1080954/150‑1 (MQ=255)
                              aTCACACATTACTACGGTGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTg                                                                                                                     <  2:593457/150‑1 (MQ=255)
                              aTCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTg                                                                                                                     <  2:315928/150‑1 (MQ=255)
                               tCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCAGGCAGTATGAATTCACTCTATATCTGa                                                                                                                    >  1:496332/1‑150 (MQ=255)
                                cacacaTTACTACGGGCATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGCTAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGAt                                                                                                                   <  1:124588/150‑1 (MQ=255)
                                aacacaTTACTACGGGGATTCATTATCTATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCAATATCTGAt                                                                                                                   <  1:1698350/149‑1 (MQ=255)
                                 acacaTTACTACGGGGATTCAGTATCGATATGCGGATGACAACCTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATa                                                                                                                  >  1:363506/1‑150 (MQ=255)
                                  cacaTTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATAc                                                                                                                 >  1:464938/1‑150 (MQ=255)
                                   acaTTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACa                                                                                                                <  1:207032/150‑1 (MQ=255)
                                      ttACTACGGGGATTCTGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGCGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACg                                                                                                             <  2:30004/150‑1 (MQ=255)
                                      ttACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACg                                                                                                             >  1:1124324/1‑150 (MQ=255)
                                      ttACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACg                                                                                                             <  2:815142/150‑1 (MQ=255)
                                         ctacGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGAt                                                                                                          >  2:1608159/1‑150 (MQ=255)
                                            cGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATccc                                                                                                       >  1:918495/1‑150 (MQ=255)
                                             ggggGTTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTACCTTATGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCCCTCAATATCAGAAACAACGGATcccc                                                                                                      >  1:414202/1‑150 (MQ=255)
                                                 aTTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTcc                                                                                                  <  2:1424486/150‑1 (MQ=255)
                                                 aTTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTcc                                                                                                  <  1:1217308/150‑1 (MQ=255)
                                                    cAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATTTCTGATACAACGTATCCCCTTCCAGc                                                                                               >  1:1204350/1‑150 (MQ=255)
                                                    cAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATAAAACGGATCCCCTTCCCGc                                                                                               >  1:181967/1‑150 (MQ=255)
                                                    cAGTAGCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACACCGGATCCCCTTCCCGc                                                                                               >  1:1163578/1‑150 (MQ=255)
                                                     aGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACAAGGATGCGATAATCTACTCAATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGcc                                                                                              >  1:546107/1‑150 (MQ=255)
                                                     aGTATAGATTTGGGGATGACAAACTGGGCTAATATTTTTACGAGGATGATATAATCTACTCCATCAAATTTAACTTCTTATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGcc                                                                                              <  2:1494010/150‑1 (MQ=255)
                                                        aTCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAg                                                                                           >  2:1035453/1‑150 (MQ=255)
                                                          cGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCCCTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGcc                                                                                         >  2:507857/1‑150 (MQ=255)
                                                            ttatGCGGATGACAAACTGGGCGAAGATTTTTAGCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCAt                                                                                       <  2:1583984/149‑1 (MQ=255)
                                                             tatGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCAtt                                                                                      >  2:876888/1‑150 (MQ=255)
                                                               tGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCAtttg                                                                                    >  1:180542/1‑148 (MQ=255)
                                                                   gATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCa                                                                                >  2:1058168/1‑150 (MQ=255)
                                                                     tGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCAtt                                                                              >  2:1733610/1‑150 (MQ=255)
                                                                     tGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCAtt                                                                              >  2:1725580/1‑150 (MQ=255)
                                                                        cAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGcc                                                                           >  1:1468125/1‑150 (MQ=255)
                                                                                 cGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGGTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCATGCTCTCg                                                                  >  2:126634/1‑150 (MQ=255)
                                                                                  gAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGt                                                                 >  1:1250066/1‑150 (MQ=255)
                                                                                     gATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTAcc                                                              <  1:611160/150‑1 (MQ=255)
                                                                                      aTTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCa                                                             >  2:1031104/1‑150 (MQ=255)
                                                                                           tACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTcc                                                        <  1:1243308/150‑1 (MQ=255)
                                                                                             ccAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCACGCCATTAGAGCATTGCCCTGGTCTCGTACCACGTCCCa                                                      >  2:215310/1‑150 (MQ=255)
                                                                                               aGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTAGTACCACGTCCCATa                                                    <  2:1374188/150‑1 (MQ=255)
                                                                                                  aTGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAg                                                 >  1:378855/1‑150 (MQ=255)
                                                                                                    gCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCt                                               >  2:1689523/1‑150 (MQ=255)
                                                                                                    gCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCt                                               >  2:1468497/1‑150 (MQ=255)
                                                                                                     cGATAATCTACTCCATCAAATTTGACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCCGGCATTAGCGCATTGCCCTGGTCTCGTACACCGTGCCATAAAGCtt                                              >  2:1803163/1‑150 (MQ=255)
                                                                                                         aaTCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAgc                                          <  1:453841/150‑1 (MQ=255)
                                                                                                            cTACTCAATAAAATTTAACTTCTGATTGATTACTGCCGTGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAgcggc                                       <  1:474199/150‑1 (MQ=255)
                                                                                                              aCTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGcggcgg                                     <  1:1457568/150‑1 (MQ=255)
                                                                                                              aCTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGcggcgg                                     <  1:1733610/150‑1 (MQ=255)
                                                                                                              aCTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGcggcgg                                     <  1:1725580/150‑1 (MQ=255)
                                                                                                              aCTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGcggcgg                                     <  1:698006/150‑1 (MQ=255)
                                                                                                                    tCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGata                               >  1:841096/1‑148 (MQ=255)
                                                                                                                     cAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAg                              <  1:1600175/150‑1 (MQ=255)
                                                                                                                       aaTTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGGCTCGTGCCACGTCCCATAAAGGTTCAGCGGCGGTGGAAAGTg                            >  2:510720/1‑150 (MQ=255)
                                                                                                                         tttAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGaa                          <  2:682503/150‑1 (MQ=255)
                                                                                                                            aaCTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACgg                       >  2:626275/1‑150 (MQ=255)
                                                                                                                              cTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGtt                     >  1:483050/1‑150 (MQ=255)
                                                                                                                                tCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGttct                   >  2:36557/1‑150 (MQ=255)
                                                                                                                                 cTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTTGCGCTTTGCCCTGGTATCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGttctt                  >  1:187785/1‑150 (MQ=255)
                                                                                                                                 cTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGttctt                  <  1:1231037/150‑1 (MQ=255)
                                                                                                                                  tgattgatTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGttcttc                 >  2:463350/1‑150 (MQ=255)
                                                                                                                                   gattgattACTGCCGGGGCTCCCGGCAGTATGACTTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATACAGCTTCAGCGGCGGTGGAAAGTGACCGGTTCTTcc                >  1:86452/1‑150 (MQ=255)
                                                                                                                                   gattgattACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTcc                <  2:1124324/150‑1 (MQ=255)
                                                                                                                                   gaattcatACTGCCGGGAGCCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCGGGTCTCGGCCCACGGCCCATAAAGCTTCAGCGGCGGGGGAAAGTGAACGGTTCTTcc                >  2:1805114/8‑150 (MQ=255)
                                                                                                                                    attgattACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCt               >  2:822751/1‑150 (MQ=255)
                                                                                                                                     ttgattACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTg              <  1:416871/150‑1 (MQ=255)
                                                                                                                                      tgattACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCCGCGGCGGTGGAAAGTGAACGGTTGTTCCTgc             >  1:703761/1‑150 (MQ=255)
                                                                                                                                      tgattACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTgc             <  1:1351939/150‑1 (MQ=255)
                                                                                                                                       gattACTGCCGGGGCTCCCGGCAGTATGAATTCCCTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTCCCACGTCCCATAAAGCTTCAGCGGCGGGGGAAAGTGAACGGTTCTTCCTgcg            >  1:1711111/1‑150 (MQ=255)
                                                                                                                                       gattACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCTGTGGAAAGTGAACGGTTCTTCCTgcg            >  2:1569164/1‑150 (MQ=255)
                                                                                                                                       gattACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTgcg            >  2:875379/1‑150 (MQ=255)
                                                                                                                                       gattACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTgcg            >  2:3715/1‑150 (MQ=255)
                                                                                                                                        catACTGCCGGGAGCCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCATTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCATCAGCGGCGGTGGAAAGTGAACGGGTCTTCCTgcgc           >  1:79073/3‑150 (MQ=255)
                                                                                                                                           aCTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCAcc        <  2:688986/150‑1 (MQ=255)
                                                                                                                                           aCTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCAGTAGCGCATTGCCCTGGTCTCGTACCACGGCCCATTAAGCTTCAGCGGCGGTGGCAAGTGCCCGGTTCTGCCTGCGCAcc        >  1:89002/1‑150 (MQ=255)
                                                                                                                                            cTGCCGGGGCTCCCGGAAGTATGAATTCGCGCTTTATCTGATACAACGGATCCCCTGCCCGACCAGCACTTAGCGCATTGACCTGGTCAAGTACCAAGTCCGATAAAGCTTCTGCGGCGGTGGAGGGTGACGGGTTCTTCCGgcgggacc       >  2:333739/1‑145 (MQ=255)
                                                                                                                                              gCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGc     >  1:1653161/1‑150 (MQ=255)
                                                                                                                                               ccGGGGCTCCCGGCAGTATGAATTAACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCGCGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCa    <  1:1566591/150‑1 (MQ=255)
                                                                                                                                                cGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCAt   <  1:628357/150‑1 (MQ=255)
                                                                                                                                                 ggggCTCCCGGCAGTATGAATTCACTCTATTTCTGATACAACGGATCCCCTTCCCGCCCCGCCATTAGCGCATTGCCCTGGTCTCGTACCCCGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCGCCCGCATc  >  1:931067/1‑150 (MQ=255)
                                                                                                                                                 ggggCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATc  >  1:1418032/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
TGGCGCAGTTGCACTGTGGTGGAATGCAACATCACACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATC  >  NC_000913/2525759‑2526053

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: