Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,555,202 G→C 6.4% intergenic (‑20/+26) eutR ← / ← eutK eut operon transcriptional activator, AraC family/putative ethanol utilization carboxysome structural protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,555,2020GC 6.4% 382.7 / 14.6 125intergenic (‑20/+26)eutR/eutKeut operon transcriptional activator, AraC family/putative ethanol utilization carboxysome structural protein
Reads supporting (aligned to +/- strand):  ref base G (54/61);  new base C (5/3);  total (60/65)
Fisher's exact test for biased strand distribution p-value = 4.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.88e-01

Only 100 of 133 total aligned reads displayed.

GTTCATAGACATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACTTCTCCTGCC  >  NC_000913/2555056‑2555351
                                                                                                                                                  |                                                                                                                                                     
 ttCATAGACATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAgg                                                                                                                                                   <  2:1042305/150‑1 (MQ=255)
   cATAGACATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGac                                                                                                                                                 <  1:819158/150‑1 (MQ=255)
     tAGACATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGacac                                                                                                                                               >  1:1192729/1‑150 (MQ=255)
       gACATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACAccc                                                                                                                                             >  2:316505/1‑150 (MQ=255)
       gACATCCGTTGTCTGTCGTTAATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACAccc                                                                                                                                             >  2:1899083/1‑150 (MQ=255)
        aCATCCGTTGGCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCg                                                                                                                                            <  1:1814776/150‑1 (MQ=255)
         cATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTGCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTCCTACAACACCCgg                                                                                                                                           <  1:1793961/150‑1 (MQ=255)
          ctCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGCCCTTCGGCGGGAGCGTCAGGGGTTCGGGTACGGGTTCAGGATAACGATGGGGCAAATTGGCTGTCCGGGGCTTTTTCATGATGTTAATGCCGGGTGTCCTACAACACCCGGc                                                                                                                                          <  1:918909/149‑1 (MQ=255)
          aTCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGGTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCGTTTTCATGATGTTAATGCCGGGTGTTGTACGACACCCGGc                                                                                                                                          >  2:101863/1‑150 (MQ=255)
          aTCCGTTGTCTGTCGTTGATGAACATTATCAACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGGCTTTTTCATGATGTTAATGCCGGGTGTCCTACAACACCCGGc                                                                                                                                          <  1:924962/150‑1 (MQ=255)
           tCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCa                                                                                                                                         >  1:177408/1‑150 (MQ=255)
           tCCGTTGTCTGGCGTTTATTAAAATTATCCACTTCGACCTTCGGCGTTAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCa                                                                                                                                         <  2:1677660/150‑1 (MQ=255)
             cgctgtctgtcGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTGCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCAcc                                                                                                                                       <  2:321126/147‑1 (MQ=255)
                 gtctgtcGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGTTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCg                                                                                                                                   >  2:1087897/1‑150 (MQ=255)
                 gtctgtcGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCg                                                                                                                                   <  1:1004996/150‑1 (MQ=255)
                   ctgtcGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTTTAGGACACCCGGCACCTCCGAc                                                                                                                                 >  2:89508/1‑150 (MQ=255)
                   ctgtcGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGAc                                                                                                                                 >  1:702826/1‑150 (MQ=255)
                       cGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACCCCCGGCACCTCCGACAGGt                                                                                                                             >  2:929657/1‑150 (MQ=255)
                       cGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGt                                                                                                                             >  2:149031/1‑150 (MQ=255)
                             ttaaCATTATCCACTTCTACCTTCGGCGTGAGCTTCTGGTTTTCGTTAAAGGTTTCATTATAAAGATGGTGAAAATTGGCTGTACGGGTCTTTTTCATGATTTTAATGCCGGGTGTTGTAGTACACCCGGCACCTCCGACAGGTTAATgg                                                                                                                       <  1:1380311/148‑1 (MQ=255)
                             tgaACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATgg                                                                                                                       <  1:494163/150‑1 (MQ=255)
                             tgaACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATgg                                                                                                                       >  1:452920/1‑150 (MQ=255)
                                   ttATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGCTGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCTCCTCCGACAGGTTAATGGGGCTTg                                                                                                                 >  1:76492/1‑150 (MQ=255)
                                   ttATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTg                                                                                                                 >  1:1788591/1‑150 (MQ=255)
                                   ttATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGGCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTg                                                                                                                 <  1:834119/150‑1 (MQ=255)
                                    tATCCACTTCGCCCTTGGGCGTGAGCTTCCGGTTTGCGGGTACGGGTTCATGATAACGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGGGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTga                                                                                                                <  1:1603692/150‑1 (MQ=255)
                                        cACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATTATAAAGATGGTGCAAATTGGCTGAACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACg                                                                                                            <  1:668852/150‑1 (MQ=255)
                                         aCTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGa                                                                                                           >  1:781033/1‑150 (MQ=255)
                                          cTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGAt                                                                                                          >  2:1680931/1‑150 (MQ=255)
                                           ttCGCCCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATa                                                                                                         <  1:734958/150‑1 (MQ=255)
                                           ttCGACCTTCGGCGTGAGCTTCATGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCTTGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATa                                                                                                         <  2:1306307/150‑1 (MQ=255)
                                            acGACCTTCGGCGTGACCTTCAGGTTATCGCGTAAGGGCTCATGATCACGATGGCGCAACTGGGCTGTCCGGGTCTTGTTCATGATGTTAATGCCGGGTGGTGAAGGACACCCGGCACCTCCGACAGGGTAATGGGGCTTGAGACGATaa                                                                                                        <  2:541662/149‑1 (MQ=255)
                                                cgttCGGCGTGAGCTTCAGGTTTTCGGGTAATGGCGCTTGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGac                                                                                                    <  1:44415/148‑1 (MQ=255)
                                                     ggCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTg                                                                                               <  2:32130/150‑1 (MQ=255)
                                                         tGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGtt                                                                                           <  2:1539744/150‑1 (MQ=255)
                                                             gttCTGGTTTTCGGTTATGGGTGCATGATAACTTTGGGGAAAATTGGCTGTACGGGTCTTTTCCATGTTGTTAATGCCGGGTGTGTTAGTACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACg                                                                                       <  1:1584610/149‑1 (MQ=255)
                                                              ttCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGt                                                                                      <  2:1482797/150‑1 (MQ=255)
                                                               tCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGTCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTa                                                                                     >  1:382425/1‑150 (MQ=255)
                                                                     tttCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTcc                                                                               >  2:1911629/1‑150 (MQ=255)
                                                                         gggTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGc                                                                           <  2:379328/150‑1 (MQ=255)
                                                                            tAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGa                                                                        <  2:678557/150‑1 (MQ=255)
                                                                             atgggTTCATGATAACGATTGGGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGTTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGaa                                                                       <  1:611068/148‑1 (MQ=255)
                                                                                gtttCATGATAATGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAag                                                                    <  1:63132/148‑1 (MQ=255)
                                                                                  tataTGAAAAAGATGGGTCAAATTGGCTGTACGGGTCTTTTTCATGATGTTTATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAagag                                                                  <  2:1762295/147‑1 (MQ=255)
                                                                                   tCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGc                                                                 <  1:167661/150‑1 (MQ=255)
                                                                                    cATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCt                                                                >  1:1748196/1‑150 (MQ=255)
                                                                                    cATGATAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTg                                                               >  2:577748/1‑150 (MQ=255)
                                                                                      tGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACTATAACGACTAATGCTTTTACGAAACGTCCCGGCAGAAAagagattt                                                              >  1:1084664/1‑146 (MQ=255)
                                                                                      tGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGt                                                              >  1:947420/1‑150 (MQ=255)
                                                                                      tGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGt                                                              >  1:197953/1‑150 (MQ=255)
                                                                                           aaGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAg                                                         <  1:150567/150‑1 (MQ=255)
                                                                                             gATGGTGCAAATTGGCTGTACGGGTCTTTTTGATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAgcg                                                       <  2:382425/150‑1 (MQ=255)
                                                                                             gATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGGGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAgcg                                                       <  1:172790/150‑1 (MQ=255)
                                                                                                ggTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCAt                                                    >  2:586273/1‑150 (MQ=255)
                                                                                                     aaaTTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTg                                               <  2:1073828/150‑1 (MQ=255)
                                                                                                      aaTTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTgg                                              <  1:667720/150‑1 (MQ=255)
                                                                                                      aaTTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTgg                                              >  2:224209/1‑150 (MQ=255)
                                                                                                        ttGGCTGTACGGGTCTTTTTCATTATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCt                                            <  2:452920/150‑1 (MQ=255)
                                                                                                          ggCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCttt                                          <  2:1115679/150‑1 (MQ=255)
                                                                                                                aCGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAg                                    <  1:1069459/150‑1 (MQ=255)
                                                                                                                   ggTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCgg                                 >  2:1246730/1‑150 (MQ=255)
                                                                                                                   ggTCTTTTTCATGATGGTAATGCCGGGTGCTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCgg                                 <  2:925407/150‑1 (MQ=255)
                                                                                                                     tCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGgcc                               >  2:458342/1‑150 (MQ=255)
                                                                                                                       tttttCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGgccag                             >  1:1742042/1‑150 (MQ=255)
                                                                                                                        ttttCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGgccagc                            >  2:1621606/1‑150 (MQ=255)
                                                                                                                         tttCATGATGTTAATGCCGGGGGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGgccagcc                           <  2:1364102/150‑1 (MQ=255)
                                                                                                                          ttCATGATCATACTGCCGGGTGTGGCCGGACACCCGGCACCTCCGACAGGGTAATGGGGCTTGAGACGAAAACGACAACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGgccagcca                          <  2:398084/150‑1 (MQ=255)
                                                                                                                           tCATGATGTTAATGCCGGGTGTTGTAGGACACCCGTCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCaa                         >  2:1416050/1‑150 (MQ=255)
                                                                                                                           tCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTTACGTCCCCGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCaa                         >  1:1044709/1‑150 (MQ=255)
                                                                                                                           tCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCaa                         <  1:244040/150‑1 (MQ=255)
                                                                                                                            cATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCaaa                        >  1:1517005/1‑150 (MQ=255)
                                                                                                                            cATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGAGCATTTCAGGCTTTTTCCAGCGGCCAGCCaaa                        >  1:1289979/1‑150 (MQ=255)
                                                                                                                             atgatgTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAg                       >  1:668307/1‑150 (MQ=255)
                                                                                                                              tgatgTTAATGCCGGGTGTTGTAGGCCAGCCGGCACCTCCGCCAGGTTAATGGGGCTTGAGACGATAAGGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGATTTTTCCAGCGGCCAGCCAAAGt                      >  1:330940/1‑150 (MQ=255)
                                                                                                                               gatgTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTAGCTTTTTCCAGCGGCCAGCCAAAGTg                     <  2:781033/150‑1 (MQ=255)
                                                                                                                                 tgTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTggg                   >  1:1795200/1‑150 (MQ=255)
                                                                                                                                 tgTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTggg                   >  1:1802517/1‑150 (MQ=255)
                                                                                                                                 tgTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCCTTTTCCAGCGGCCAGCCAAAGTggg                   >  1:462678/1‑150 (MQ=255)
                                                                                                                                   ttAATGGCGGGGGGTGTCGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCAGGCTTTTTCCAGCGGCCAGCCAACGTGGGCa                 <  1:1483561/150‑1 (MQ=255)
                                                                                                                                   ttAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCa                 <  1:929657/150‑1 (MQ=255)
                                                                                                                                   ttAATGCCGGGGGGTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCATGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGGGGGCa                 <  1:316505/150‑1 (MQ=255)
                                                                                                                                     ggtGCCGGGTGTCCTACAACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAGCGTCCCGTCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGGAGc               >  2:264649/3‑150 (MQ=255)
                                                                                                                                     aaTGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCCGc               >  2:75346/1‑150 (MQ=255)
                                                                                                                                      gtGCCGGGTGTCCTACAACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGcc              >  2:23651/2‑150 (MQ=255)
                                                                                                                                      gtGCCGGGTGTCCTACAACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGcc              >  2:1605215/2‑150 (MQ=255)
                                                                                                                                      aTGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGcc              <  1:1265000/150‑1 (MQ=255)
                                                                                                                                        gCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCAc            <  1:574571/150‑1 (MQ=255)
                                                                                                                                         ccGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAATAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACt           <  2:76492/150‑1 (MQ=255)
                                                                                                                                         ccGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACt           <  2:1788591/150‑1 (MQ=255)
                                                                                                                                         ccGGGTGTCCTACAACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGCGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACt           >  2:233013/1‑150 (MQ=255)
                                                                                                                                          cGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACtt          >  1:1736492/1‑150 (MQ=255)
                                                                                                                                           gggTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACTtc         >  2:1593740/1‑150 (MQ=255)
                                                                                                                                           gggTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACTtc         <  2:1231490/150‑1 (MQ=255)
                                                                                                                                            ggTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACTtct        <  2:1256574/150‑1 (MQ=255)
                                                                                                                                               gttgtAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACTTCTCCt     >  1:616825/1‑150 (MQ=255)
                                                                                                                                                 tgtAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTATACGTAACGTCCCGGCAGAAAAGAGCTGGTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACTGCTCCTGc   >  1:1748232/1‑150 (MQ=255)
                                                                                                                                                 tgtAGCCCACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACTTCTCCTGc   <  1:489306/150‑1 (MQ=255)
                                                                                                                                                  gtAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTTTTCGAGCGCATTTATGGCTTTTTCCAGCTGCCAGCCAAAGTGGGCAGCCACTTCTACTGcc  >  2:1660690/1‑150 (MQ=255)
                                                                                                                                                  gtAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACTTCTCCTGcc  >  2:1699099/1‑150 (MQ=255)
                                                                                                                                                  gtAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGAAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACTTCTCCTGcc  >  1:28101/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
GTTCATAGACATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACTTCTCCTGCC  >  NC_000913/2555056‑2555351

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: