Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,555,205 G→C 6.6% intergenic (‑23/+23) eutR ← / ← eutK eut operon transcriptional activator, AraC family/putative ethanol utilization carboxysome structural protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,555,2050GC 6.6% 398.0 / 16.4 133intergenic (‑23/+23)eutR/eutKeut operon transcriptional activator, AraC family/putative ethanol utilization carboxysome structural protein
Reads supporting (aligned to +/- strand):  ref base G (63/61);  new base C (6/3);  total (69/64)
Fisher's exact test for biased strand distribution p-value = 4.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.30e-01

Only 100 of 139 total aligned reads displayed.

GTTCATAGACATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACTTCTCCTGCCGTC  >  NC_000913/2555056‑2555354
                                                                                                                                                     |                                                                                                                                                     
   cATAGACATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGac                                                                                                                                                    <  1:819158/150‑1 (MQ=255)
     tAGACATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGacac                                                                                                                                                  >  1:1192729/1‑150 (MQ=255)
       gACATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACAccc                                                                                                                                                >  2:316505/1‑150 (MQ=255)
       gACATCCGTTGTCTGTCGTTAATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACAccc                                                                                                                                                >  2:1899083/1‑150 (MQ=255)
        aCATCCGTTGGCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCg                                                                                                                                               <  1:1814776/150‑1 (MQ=255)
         cATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTGCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTCCTACAACACCCgg                                                                                                                                              <  1:1793961/150‑1 (MQ=255)
          ctCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGCCCTTCGGCGGGAGCGTCAGGGGTTCGGGTACGGGTTCAGGATAACGATGGGGCAAATTGGCTGTCCGGGGCTTTTTCATGATGTTAATGCCGGGTGTCCTACAACACCCGGc                                                                                                                                             <  1:918909/149‑1 (MQ=255)
          aTCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGGTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCGTTTTCATGATGTTAATGCCGGGTGTTGTACGACACCCGGc                                                                                                                                             >  2:101863/1‑150 (MQ=255)
          aTCCGTTGTCTGTCGTTGATGAACATTATCAACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGGCTTTTTCATGATGTTAATGCCGGGTGTCCTACAACACCCGGc                                                                                                                                             <  1:924962/150‑1 (MQ=255)
           tCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCa                                                                                                                                            >  1:177408/1‑150 (MQ=255)
           tCCGTTGTCTGGCGTTTATTAAAATTATCCACTTCGACCTTCGGCGTTAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCa                                                                                                                                            <  2:1677660/150‑1 (MQ=255)
            ccGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCAc                                                                                                                                           >  2:748233/1‑150 (MQ=255)
             cGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTGCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCAcc                                                                                                                                          >  2:1141791/1‑150 (MQ=255)
                 gtctgtcGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCg                                                                                                                                      <  1:1004996/150‑1 (MQ=255)
                      tcGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTCCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAgg                                                                                                                                 <  2:550507/150‑1 (MQ=255)
                       cGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACCCCCGGCACCTCCGACAGGt                                                                                                                                >  2:929657/1‑150 (MQ=255)
                       cGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGt                                                                                                                                >  2:149031/1‑150 (MQ=255)
                             ttaaCATTATCCACTTCTACCTTCGGCGTGAGCTTCTGGTTTTCGTTAAAGGTTTCATTATAAAGATGGTGAAAATTGGCTGTACGGGTCTTTTTCATGATTTTAATGCCGGGTGTTGTAGTACACCCGGCACCTCCGACAGGTTAATgg                                                                                                                          <  1:1380311/148‑1 (MQ=255)
                             tgaACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCTTGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATgg                                                                                                                          >  1:296637/1‑150 (MQ=255)
                             tgaACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATgg                                                                                                                          >  2:1050466/1‑150 (MQ=255)
                              gaACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATggg                                                                                                                         >  2:434841/1‑150 (MQ=255)
                                   ttATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGCTGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCTCCTCCGACAGGTTAATGGGGCTTg                                                                                                                    >  1:76492/1‑150 (MQ=255)
                                   ttATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTg                                                                                                                    >  1:1788591/1‑150 (MQ=255)
                                   ttATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGGCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTg                                                                                                                    <  1:834119/150‑1 (MQ=255)
                                    tATCCACTTCGCCCTTGGGCGTGAGCTTCCGGTTTGCGGGTACGGGTTCATGATAACGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGGGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTga                                                                                                                   <  1:1603692/150‑1 (MQ=255)
                                        cACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATTATAAAGATGGTGCAAATTGGCTGAACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACg                                                                                                               <  1:668852/150‑1 (MQ=255)
                                        cACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACg                                                                                                               >  1:1326429/1‑150 (MQ=255)
                                         aCTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGa                                                                                                              >  1:781033/1‑150 (MQ=255)
                                          cTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGAt                                                                                                             >  1:577524/1‑150 (MQ=255)
                                           ttCGCCCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATa                                                                                                            <  1:734958/150‑1 (MQ=255)
                                           ttCGACCTTCGGCGTGAGCTTCATGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCTTGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATa                                                                                                            <  2:1306307/150‑1 (MQ=255)
                                                cgttCGGCGTGAGCTTCAGGTTTTCGGGTAATGGCGCTTGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGac                                                                                                       <  1:44415/148‑1 (MQ=255)
                                                     ggCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTg                                                                                                  <  2:32130/150‑1 (MQ=255)
                                                              ttCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGt                                                                                         <  2:1482797/150‑1 (MQ=255)
                                                                     tttCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTcc                                                                                  >  1:1740309/1‑150 (MQ=255)
                                                                            tAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACGCCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACTACTACTGCGTTTACGTAACGTCCCGGCAGa                                                                           >  2:365899/1‑150 (MQ=255)
                                                                                gtttCATGATAATGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAag                                                                       <  1:63132/148‑1 (MQ=255)
                                                                                  tataTGAAAAAGATGGGTCAAATTGGCTGTACGGGTCTTTTTCATGATGTTTATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAagag                                                                     <  2:1762295/147‑1 (MQ=255)
                                                                                    cATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCt                                                                   >  1:1748196/1‑150 (MQ=255)
                                                                                    cATGATAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTg                                                                  >  2:577748/1‑150 (MQ=255)
                                                                                      tGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGt                                                                 >  1:947420/1‑150 (MQ=255)
                                                                                      tGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGt                                                                 >  1:197953/1‑150 (MQ=255)
                                                                                           aaGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAg                                                            <  1:150567/150‑1 (MQ=255)
                                                                                            aGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAgc                                                           >  1:1619802/1‑150 (MQ=255)
                                                                                             gATGGTGCAAATTGGCTGTACGGGTCTTTTTGATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAgcg                                                          <  2:382425/150‑1 (MQ=255)
                                                                                             gATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGGGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAgcg                                                          <  1:172790/150‑1 (MQ=255)
                                                                                                ggTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCAt                                                       >  2:586273/1‑150 (MQ=255)
                                                                                                ggTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCAt                                                       <  2:229864/150‑1 (MQ=255)
                                                                                                  tGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCAttt                                                     >  2:1574296/1‑150 (MQ=255)
                                                                                                     aaaTTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTg                                                  <  2:1073828/150‑1 (MQ=255)
                                                                                                      aaTTGGCTTTTCGGGTGTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGTCAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGATAAGAGCTGTTCGAGCGCATTTCTgg                                                 <  2:1881979/150‑1 (MQ=255)
                                                                                                      aaTTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTgg                                                 >  2:224209/1‑150 (MQ=255)
                                                                                                      aaTTGGCTGGACGGGGCTTTTTCATGATGTTAATGCCGGGGGTTGTTGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTgg                                                 <  1:674390/150‑1 (MQ=255)
                                                                                                        ttGGCTGTACGGGTCTTTTTCATTATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCt                                               <  2:452920/150‑1 (MQ=255)
                                                                                                          ggCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCttt                                             <  2:1115679/150‑1 (MQ=255)
                                                                                                             tggaCGGGTCTTTTTCATGAGGTTAATGCCGGGTGGTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATCACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTc                                          <  1:15156/147‑1 (MQ=255)
                                                                                                               tACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCa                                        <  1:434841/150‑1 (MQ=255)
                                                                                                                aCGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAg                                       <  1:1069459/150‑1 (MQ=255)
                                                                                                                  gggTCTTTTTCTGGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCg                                     <  2:1681700/150‑1 (MQ=255)
                                                                                                                   ggTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCgg                                    >  2:1246730/1‑150 (MQ=255)
                                                                                                                   ggTCTTTTTCATGATGGTAATGCCGGGTGCTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCgg                                    <  2:925407/150‑1 (MQ=255)
                                                                                                                     tCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGgcc                                  >  2:458342/1‑150 (MQ=255)
                                                                                                                        ttttCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGgccagc                               >  2:1621606/1‑150 (MQ=255)
                                                                                                                         tttCATGATGTTAATGCCGGGGGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGgccagcc                              <  2:1364102/150‑1 (MQ=255)
                                                                                                                          ttCATGATGTTAAGGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGgccagcca                             <  1:470271/150‑1 (MQ=255)
                                                                                                                          ttCATGATCATACTGCCGGGTGTGGCCGGACACCCGGCACCTCCGACAGGGTAATGGGGCTTGAGACGAAAACGACAACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGgccagcca                             <  2:398084/150‑1 (MQ=255)
                                                                                                                           tCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTTACGTCCCCGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCaa                            >  1:1044709/1‑150 (MQ=255)
                                                                                                                           tCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCaa                            <  1:244040/150‑1 (MQ=255)
                                                                                                                            cATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCaaa                           >  1:1517005/1‑150 (MQ=255)
                                                                                                                            atttatgTTAATGCCGGGGGTTGTAGGCCACATGGAACCTACGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCTTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCaaa                           <  1:943105/146‑1 (MQ=255)
                                                                                                                             atgatgTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAg                          >  1:668307/1‑150 (MQ=255)
                                                                                                                              tgatgTTAATGCCGGGTGTTGTAGGCCAGCCGGCACCTCCGCCAGGTTAATGGGGCTTGAGACGATAAGGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGATTTTTCCAGCGGCCAGCCAAAGt                         >  1:330940/1‑150 (MQ=255)
                                                                                                                               gatgTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTAGCTTTTTCCAGCGGCCAGCCAAAGTg                        <  2:781033/150‑1 (MQ=255)
                                                                                                                                 tgTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTggg                      >  1:1795200/1‑150 (MQ=255)
                                                                                                                                 tgTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTggg                      >  1:1802517/1‑150 (MQ=255)
                                                                                                                                 tgTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCCTTTTCCAGCGGCCAGCCAAAGTggg                      >  1:462678/1‑150 (MQ=255)
                                                                                                                                   ttAATGCCGGGGGGTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCATGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGGGGGCa                    <  1:316505/150‑1 (MQ=255)
                                                                                                                                     ggtGCCGGGTGTCCTACAACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAGCGTCCCGTCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGGAGc                  >  2:264649/3‑150 (MQ=255)
                                                                                                                                     aaTGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCCGc                  >  2:75346/1‑150 (MQ=255)
                                                                                                                                      gtGCCGGGTGTCCTACAACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGcc                 >  2:1605215/2‑150 (MQ=255)
                                                                                                                                      aTGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGcc                 <  1:1265000/150‑1 (MQ=255)
                                                                                                                                        gCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCAc               <  1:574571/150‑1 (MQ=255)
                                                                                                                                        gCCGGGTGTCCTACAACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCAc               >  1:662078/1‑150 (MQ=255)
                                                                                                                                         ccGGGTGTCCTACAACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGCGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACt              >  2:233013/1‑150 (MQ=255)
                                                                                                                                         ccGGGGGGTGTCGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACt              <  1:113680/150‑1 (MQ=255)
                                                                                                                                          cGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACtt             >  1:1736492/1‑150 (MQ=255)
                                                                                                                                           gggTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCTGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACTtc            >  2:790012/1‑150 (MQ=255)
                                                                                                                                            ggTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACTtct           <  2:1256574/150‑1 (MQ=255)
                                                                                                                                               gttgtAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACTTCTCCt        >  1:616825/1‑150 (MQ=255)
                                                                                                                                                 tgtAGGACACCCGGCACCTCCGACAGGTTAATGGTGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGAGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTCTTCAGCCACTTCTACTGc      >  2:292715/1‑150 (MQ=255)
                                                                                                                                                 tgtAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTGCAGCGGCCAGCCAAAGTGGGCAGCCACTTCTCCTGc      >  2:1431012/1‑150 (MQ=255)
                                                                                                                                                 tgtAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTATACGTAACGTCCCGGCAGAAAAGAGCTGGTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACTGCTCCTGc      >  1:1748232/1‑150 (MQ=255)
                                                                                                                                                 tgtAGCCCACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACTTCTCCTGc      <  1:489306/150‑1 (MQ=255)
                                                                                                                                                 ggtcggACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACTTCTCCTGc      <  2:1405301/146‑1 (MQ=255)
                                                                                                                                                  gtAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTTTTCGAGCGCATTTATGGCTTTTTCCAGCTGCCAGCCAAAGTGGGCAGCCACTTCTACTGcc     >  2:1660690/1‑150 (MQ=255)
                                                                                                                                                  gtAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACTTCTCCTGcc     >  2:1699099/1‑150 (MQ=255)
                                                                                                                                                    aGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACTTCTCCTGCCGt   >  2:1123452/1‑150 (MQ=255)
                                                                                                                                                     ggACACCCGGCACCTCCGCCAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACTTCTCCTGCCGTc  >  2:814724/1‑150 (MQ=255)
                                                                                                                                                     ggACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGCGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACTTCTCCTGCCGTc  >  2:1600673/1‑150 (MQ=255)
                                                                                                                                                     ggACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACTTCTCCTGCCGTc  <  2:1587227/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GTTCATAGACATCCGTTGTCTGTCGTTGATGAACATTATCCACTTCGACCTTCGGCGTGAGCTTCAGGTTTTCGGGTAAGGGTTCATGATAAAGATGGTGCAAATTGGCTGTACGGGTCTTTTTCATGATGTTAATGCCGGGTGTTGTAGGACACCCGGCACCTCCGACAGGTTAATGGGGCTTGAGACGATAACGACTACTGCGTTTACGTAACGTCCCGGCAGAAAAGAGCTGTTCGAGCGCATTTCTGGCTTTTTCCAGCGGCCAGCCAAAGTGGGCAGCCACTTCTCCTGCCGTC  >  NC_000913/2555056‑2555354

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: