Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,742,795 A→T 5.1% E138V (GAA→GTA) ‡ yfiN → putative membrane‑anchored diguanylate cyclase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,742,7950AT 5.1% 392.7 / 5.9 129E138V (GAA→GTA) ‡yfiNputative membrane‑anchored diguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base A (63/59);  new base T (4/3);  total (67/62)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 131 total aligned reads displayed.

TTTCAACTGCAGAAGTACGTGATAAGCAGCAAAATATTCTGGCATCCTGGCATTACACCCGTAAGGATCCAGGCGATACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAG  >  NC_000913/2742648‑2742944
                                                                                                                                                    |                                                                                                                                                     
tttCAACTGCAGAAGTACGTGATAAGCAGCAAAATATTCTGGCATCCTGGCATTACACCCGTAAGGATCCAGGCGATACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGaaa                                                                                                                                                     <  2:846423/150‑1 (MQ=255)
 ttCAACTGCAGAAGTACGTGATAAGCAGCAAAATATTCTGGCATCCTGGCATTACACCCGTAAGGATCCAGGCGATACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAAc                                                                                                                                                    <  2:1125442/150‑1 (MQ=255)
    aaCTGCAGAAGTACGTGATAAGCAGCAATATATTCTGGCATCCTGGCATTACACCCGTAAGGATCCAGGCGATACTATCAGTTATTTCATAGGCCAGTGGCTCTTCCCTGCCCCAATCAGTCAGCAGATTCGTCAAATTGGTGTAAACAt                                                                                                                                                 >  2:1145617/1‑150 (MQ=255)
    aaCTGCAGAAGTACGTGATAAGCAGCAAAATATTCTGGCATCCTGGCATTACACCCGTAAGGATCCAGGCGATACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCAt                                                                                                                                                 >  2:444215/1‑150 (MQ=255)
      cTGCAGAAGTACGTGATAAGCAGCAAAATATTCTGGCATCCTGGCATTACACCCGTAAGGATCCAGGCGATACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTg                                                                                                                                               <  2:1266151/150‑1 (MQ=255)
        gCAGAAGTACGTGATAAGCAGCAAAATATTCTGGCATCCTGGCATTACACCCGTAAGGATCCAGGCGATACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGc                                                                                                                                             >  1:1894780/1‑150 (MQ=255)
        gCAGAAGTACGTGATAAGCAGCAAAATATTCTGGCATCCTGGCATTACACCCGTAAGGATCCAGGCGATACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGc                                                                                                                                             <  2:1248380/150‑1 (MQ=255)
         cAGAAGTACGTGATAAGCAGCAAAATATTCTGGCATCCTGGCATTACACCCGTAAGGATCCAGGCGATACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCTTCACAATGGTGAAACCATTGGCg                                                                                                                                            >  1:201161/1‑150 (MQ=255)
          aGAAGTACGTGATAAGCAGCAAAATATTCTGGCATCCTGGCATTACACCCGTAAGGATCCAGGCGATACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTTTCACCATTgtgac                                                                                                                                           <  1:758442/150‑5 (MQ=255)
          aGAAGTACGTGATAAGCAGCAAAATATTCTGGCATCCTGGCATTACACCCGTAAGGATCCAGGCGATACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTTTCACCATTgtgac                                                                                                                                           <  1:1508374/150‑5 (MQ=255)
          aGAAGTACGTGATAAGCAGCAAAATATTCTGGCATCCTGGCATTACACCCGTAAGGATCCAGGCGATACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTTTCACCATTgtgac                                                                                                                                           <  1:257593/150‑5 (MQ=255)
          aGAAGTACGTGATAAGCAGCAAAATATTCTGGCATCCTGGCATTACACCCGTAAGGATCCAGGCGATACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGa                                                                                                                                           <  1:859620/150‑1 (MQ=255)
          aGAAGTACGTGATAAGCAGCAAAATATTCTGGCATCCTGGCATTACACCCGTAAGGATCCAGGCGATACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGa                                                                                                                                           <  2:1432828/150‑1 (MQ=255)
             aGTACGTGATAAGCAGCAAAATATTCTGGCATCCTGGCATTACACCCGTAAGGATCCAGGCGATACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCCCAATGGTGACACCATTGGCGAAGt                                                                                                                                        >  1:1207279/1‑150 (MQ=255)
                   tGATAAGCAGCAAAATATTCTGGCATCCTGGCATTACACCCGTAAGGATCCAGGCGATACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCtt                                                                                                                                  >  1:1065523/1‑150 (MQ=255)
                   tGATAAGCAGCAAAATATTCTGGCATCCTGGCATTACACCCGTAAGGATCCAGGCGATACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCtt                                                                                                                                  >  1:1303823/1‑150 (MQ=255)
                   tGATAAGCAGCAAAATATTCTGGCATCCTGGCATTACACCCGTAAGGATCCAGGCGATACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCtt                                                                                                                                  >  2:1854641/1‑150 (MQ=255)
                      tAAGCAGCAAAATATTCTGGCATCCTGGCATTACACCCGTAAGGATCCAGGCGATACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAAc                                                                                                                               <  1:199437/150‑1 (MQ=255)
                                  tatTCTGGCATCCTGGCATTACACCCGTAAGGATCCAGGCGATACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAg                                                                                                                   <  1:1882902/150‑1 (MQ=255)
                                      cTGGCATCCTGGCATTACACCCGTAAGGATCCAGGCGATACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCa                                                                                                               <  1:989064/150‑1 (MQ=255)
                                       tGGCATCCTGGCATTACACCCGTAAGGATCCAGGCGATACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCaa                                                                                                              <  1:1837726/150‑1 (MQ=255)
                                          cATCCTGGCATTACACCCGTAAGGATCCAGGCGATACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCa                                                                                                           >  1:230726/1‑150 (MQ=255)
                                           aTCCTGGCATTACACCCGTAAGGATCCAGGCGATACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAg                                                                                                          <  2:1172223/150‑1 (MQ=255)
                                              cTGGCATTACACCCGTAAGGATCCAGGCGATACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCa                                                                                                       >  1:95589/1‑150 (MQ=255)
                                              cTGGCATTACACCCGTAAGGATCCAGGCGATACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCa                                                                                                       >  1:1300758/1‑150 (MQ=255)
                                              cTGGCATTACACCCGTAAGGATCCAGGCGATACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCACATCATTCAGCCGATTCTTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACACTTCAAGCAGCCa                                                                                                       >  1:559234/1‑150 (MQ=255)
                                                 gCATTACACCCGTAAGGATCCAGGCGATACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCAttt                                                                                                    <  1:1257127/150‑1 (MQ=255)
                                                 gCATTACACCCGTAAGGATCCAGGCGATACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGGGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCAttt                                                                                                    <  2:1188565/150‑1 (MQ=255)
                                                      acacCCGTAAGGATCCAGGCGATACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATAAGCCATTTTATCt                                                                                               >  2:122232/1‑150 (MQ=255)
                                                       cacCCGTAAGGATCCAGGCGATACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTg                                                                                              <  2:977310/150‑1 (MQ=255)
                                                           cGTAAGGATCCAGGCGATACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGttt                                                                                          >  1:1829272/1‑150 (MQ=255)
                                                            gTAAGGATCCAGGCGATACTTTCAGTAATTTCAAAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGtttt                                                                                         <  2:87827/150‑1 (MQ=255)
                                                             tAAGGCTACAGGCGATACGTTCAGTAATTTCATAAGCCAATGGCTCTTACCTGCCCCCATCATACAGCCGAATGGTCACAATGGTTAAACCATTGGCGAAGTACGCGTAACCGCGCGCGACATTAGAATCAGCCCTTTTATATGGgtttg                                                                                        >  2:1747189/1‑149 (MQ=255)
                                                             tAAGGATCCAGGCGATACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTtc                                                                                        <  1:1684587/150‑1 (MQ=255)
                                                             tAAGGATCCAGGCGATACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTtc                                                                                        <  1:377621/150‑1 (MQ=255)
                                                             tAAGGATCCAGGCGATACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTtc                                                                                        <  2:1643792/150‑1 (MQ=255)
                                                             tAAGGATCCAGGCGATACTTTCAGTAATTTCATAAGCAACTGTCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTtc                                                                                        <  1:1460216/150‑1 (MQ=255)
                                                               aGGATCCAGGCGATACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTtcgc                                                                                      <  2:512702/150‑1 (MQ=255)
                                                                 gATCCAGGCGATACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTtcgctc                                                                                    <  2:1147998/150‑1 (MQ=255)
                                                                 gATCCAGGCGATACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTtcgctc                                                                                    >  2:596274/1‑150 (MQ=255)
                                                                  aTCCAGGCGATACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTtcgctcg                                                                                   >  2:512395/1‑150 (MQ=255)
                                                                      aGGCGATACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGt                                                                               >  2:1062046/1‑150 (MQ=255)
                                                                        gCGATACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTAc                                                                             <  1:436518/150‑1 (MQ=255)
                                                                          gATACTTTCAGTAATTTCATTAGCCACTTGCTCTTCCCTGCCCCCATAATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTg                                                                           <  1:272990/150‑1 (MQ=255)
                                                                              cTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCg                                                                       >  1:678373/1‑150 (MQ=255)
                                                                              cTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATAGGCGAAGTACGCTTAACCGCGCGCGACCTGGCAATAAGCCTAGTTATCTGGTTTTCGCTCGCCGTAGTGACCg                                                                       >  1:317642/1‑150 (MQ=255)
                                                                                ttCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAAAGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCAGATTATCTTGTTTTCGCTCTCCGTACTGACCgtt                                                                     >  2:582704/1‑148 (MQ=255)
                                                                                  cAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTg                                                                   <  1:1619660/150‑1 (MQ=255)
                                                                                      aaTTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTAtt                                                               <  2:988102/150‑1 (MQ=255)
                                                                                       aTTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTc                                                              >  1:554151/1‑150 (MQ=255)
                                                                                           cATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGCTTCGTCACACTGGTGAAACCATTGTCGAAGTACGCTTAACCGCTCGCGACATTTCAATCAGCCATTTAATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTctgct                                                          <  1:255855/150‑1 (MQ=255)
                                                                                           cATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTctgct                                                          <  2:1140738/150‑1 (MQ=255)
                                                                                           cATAAGCCACAGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTctgct                                                          >  2:739593/1‑150 (MQ=255)
                                                                                            aTAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGATTGTATTctgctg                                                         <  1:1615242/150‑1 (MQ=255)
                                                                                             tAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTgg                                                        <  1:611059/150‑1 (MQ=255)
                                                                                               aGCCACTGGCTCTTCCCTGCCCCCCATCATTCAGCCGATTCGTCACAATGGTGACACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGc                                                       >  2:486974/1‑150 (MQ=255)
                                                                                                 ccACTGGCTCTTCCCTGCCCCCATCAATCAGCCGATTCGTCACAATGGAGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCACGCAGTACTGACCGGATGTATTCTGCTGGCATc                                                    >  2:1170508/1‑150 (MQ=255)
                                                                                                  cACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCa                                                   >  1:1224107/1‑150 (MQ=255)
                                                                                                   aCTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAg                                                  >  1:536780/1‑150 (MQ=255)
                                                                                                    cTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAgg                                                 <  2:487802/150‑1 (MQ=255)
                                                                                                    cTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAgg                                                 >  1:1515539/1‑150 (MQ=255)
                                                                                                         tctTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCAGCAGGCATCg                                            >  2:1627421/1‑150 (MQ=255)
                                                                                                          ctTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTGCGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGc                                           >  1:571008/1‑150 (MQ=255)
                                                                                                            tCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCaa                                         >  1:1011091/1‑150 (MQ=255)
                                                                                                            tCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCaa                                         >  1:723347/1‑150 (MQ=255)
                                                                                                            tCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCaa                                         >  2:1077426/1‑150 (MQ=255)
                                                                                                            accatGCCCACATCATTCATCCGATTCTTCACAATGGTGAAACCATTGGCGAAGTACGCTTAAACGCTCGCGACAGATCAATCAGCCATTATATCTGGTTTTCGCTCTCCGTACTGACCCCTTGTATTCTGCATGCATCACGCAAAGCaa                                         >  2:860280/5‑150 (MQ=255)
                                                                                                             cccTGCCCCCATCATTCAGCCGATTCGTCACAATGGGGAATCCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCTATCAGCCTTTTTATCTGGGTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAAt                                        <  1:28182/150‑1 (MQ=255)
                                                                                                              ccTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAAtt                                       >  1:837843/1‑150 (MQ=255)
                                                                                                              ccTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAAtt                                       >  1:1621780/1‑150 (MQ=255)
                                                                                                                   cacccATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTAccc                                   >  2:133673/3‑150 (MQ=255)
                                                                                                                    ccccATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCt                                  <  2:1288022/150‑1 (MQ=255)
                                                                                                                    ccccATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCt                                  <  2:1682067/150‑1 (MQ=255)
                                                                                                                          catTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCg                            <  2:69485/150‑1 (MQ=255)
                                                                                                                                gCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCAttt                      <  2:422049/150‑1 (MQ=255)
                                                                                                                                gCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCAttt                      <  2:1894780/150‑1 (MQ=255)
                                                                                                                                 ccGATTCGTCACTATGGTGATACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGTCCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTg                     <  2:215848/150‑1 (MQ=255)
                                                                                                                                 ccGATTCGTCACAATTGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTg                     <  2:1065523/150‑1 (MQ=255)
                                                                                                                                 ccGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTg                     <  2:1300758/150‑1 (MQ=255)
                                                                                                                                      cttcgccaATGGTTTCACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACaa                >  1:44330/7‑150 (MQ=255)
                                                                                                                                        gTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCTATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATg              >  1:1046792/1‑150 (MQ=255)
                                                                                                                                        gTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGGTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATg              >  1:1159305/1‑150 (MQ=255)
                                                                                                                                         tCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATgg             >  2:1759140/1‑150 (MQ=255)
                                                                                                                                         tCACAATGGTGAAACCATTGGCGAAGTACGCGTAACCGCTCGCGACAGTTCAATCAGCCATTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGc            <  2:1708425/150‑1 (MQ=255)
                                                                                                                                          gccaATGGTTTCACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTCCTGACCGGTTGTATTCTGCTGGCCTCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGc            >  1:1729310/3‑150 (MQ=255)
                                                                                                                                          gccaATGGTTTCACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGc            >  1:632513/3‑150 (MQ=255)
                                                                                                                                          cacaATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTAAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATACGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGc            >  2:953703/1‑150 (MQ=255)
                                                                                                                                           acaTTGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTTTTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGcc           <  1:1747189/150‑1 (MQ=255)
                                                                                                                                            caATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCt          <  2:201161/150‑1 (MQ=255)
                                                                                                                                            caATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCt          >  1:872885/1‑150 (MQ=255)
                                                                                                                                            caATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCt          >  1:870777/1‑150 (MQ=255)
                                                                                                                                              aTGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTgg        <  2:1043987/150‑1 (MQ=255)
                                                                                                                                               tGGGGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGt       <  2:1612985/150‑1 (MQ=255)
                                                                                                                                                ggTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTa      >  1:406682/1‑150 (MQ=255)
                                                                                                                                                ggTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTa      <  1:787117/150‑1 (MQ=255)
                                                                                                                                                 gTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAg     >  2:576546/1‑150 (MQ=255)
                                                                                                                                                 gTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGACTGGTAg     >  2:77570/1‑150 (MQ=255)
                                                                                                                                                  tGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATATGGTTTTCCCTCGCCGTACTGACCGGTGGTATTCTGCTGGCATCAGGCATCGCAAGTACCCTCACCCGCCATTTGGACAATGGCCTGGtggc    >  2:335584/1‑147 (MQ=255)
                                                                                                                                                   gAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGaa   >  1:1304606/1‑150 (MQ=255)
                                                                                                                                                    aaaCCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAg  >  2:1239832/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TTTCAACTGCAGAAGTACGTGATAAGCAGCAAAATATTCTGGCATCCTGGCATTACACCCGTAAGGATCCAGGCGATACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAG  >  NC_000913/2742648‑2742944

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: